##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936764.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	607548
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.10689361169817	33.0	31.0	34.0	30.0	34.0
2	32.21196843706176	33.0	31.0	34.0	30.0	34.0
3	32.15864096334775	33.0	31.0	34.0	30.0	34.0
4	35.838001277265334	37.0	35.0	37.0	35.0	37.0
5	30.903661932884315	37.0	35.0	37.0	0.0	37.0
6	33.29387636861614	37.0	35.0	37.0	17.0	37.0
7	35.25239322654342	37.0	35.0	37.0	32.0	37.0
8	35.66779579555854	37.0	35.0	37.0	35.0	37.0
9	37.67375581847031	39.0	37.0	39.0	35.0	39.0
10	37.436166360518015	39.0	37.0	39.0	35.0	39.0
11	37.22381112274257	39.0	37.0	39.0	34.0	39.0
12	37.05564334011469	39.0	37.0	39.0	34.0	39.0
13	36.88427910222731	39.0	35.0	39.0	33.0	39.0
14	38.08992705103136	40.0	37.0	41.0	33.0	41.0
15	38.16963268745844	40.0	37.0	41.0	33.0	41.0
16	38.17381013516628	40.0	37.0	41.0	34.0	41.0
17	38.13770270003358	40.0	37.0	41.0	34.0	41.0
18	38.08710587476216	40.0	36.0	41.0	34.0	41.0
19	38.04033096973408	40.0	36.0	41.0	34.0	41.0
20	38.02383679972611	40.0	36.0	41.0	34.0	41.0
21	37.974189693653834	40.0	36.0	41.0	33.0	41.0
22	37.980676423920414	40.0	36.0	41.0	34.0	41.0
23	37.973583980195805	40.0	36.0	41.0	34.0	41.0
24	37.95819754159342	40.0	36.0	41.0	34.0	41.0
25	37.79390599590485	40.0	36.0	41.0	33.0	41.0
26	37.71349259646974	40.0	36.0	41.0	33.0	41.0
27	37.66246123763061	40.0	36.0	41.0	33.0	41.0
28	37.630875255946854	40.0	36.0	41.0	33.0	41.0
29	37.59729766207773	40.0	36.0	41.0	33.0	41.0
30	37.51505230862418	40.0	36.0	41.0	33.0	41.0
31	37.43919821972914	40.0	35.0	41.0	33.0	41.0
32	37.34251779283283	40.0	35.0	41.0	33.0	41.0
33	37.24187553905206	39.0	35.0	41.0	32.0	41.0
34	37.121157505250615	39.0	35.0	41.0	32.0	41.0
35	37.06951384911151	39.0	35.0	41.0	32.0	41.0
36	36.961206357357774	39.0	35.0	41.0	31.0	41.0
37	36.947584388394006	39.0	35.0	41.0	31.0	41.0
38	36.89719989202499	39.0	35.0	41.0	31.0	41.0
39	36.80623588588885	39.0	35.0	41.0	31.0	41.0
40	36.702133823171174	39.0	35.0	41.0	31.0	41.0
41	36.61085543858263	39.0	35.0	41.0	31.0	41.0
42	36.590177237024896	39.0	35.0	41.0	31.0	41.0
43	36.51289774635091	39.0	35.0	41.0	31.0	41.0
44	36.36091633912053	39.0	35.0	40.0	30.0	41.0
45	36.28988985232443	38.0	35.0	40.0	30.0	41.0
46	36.14800312074108	38.0	35.0	40.0	30.0	41.0
47	36.06942496724539	38.0	35.0	40.0	30.0	41.0
48	35.96822967074206	38.0	35.0	40.0	30.0	41.0
49	35.86919881227492	38.0	35.0	40.0	30.0	41.0
50	35.688791667489646	38.0	35.0	40.0	29.0	41.0
51	33.98977364751427	36.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	13.0
9	31.0
10	59.0
11	28.0
12	20.0
13	10.0
14	19.0
15	29.0
16	58.0
17	117.0
18	181.0
19	329.0
20	547.0
21	828.0
22	1207.0
23	1797.0
24	2307.0
25	3261.0
26	4157.0
27	5258.0
28	6301.0
29	7654.0
30	9984.0
31	13243.0
32	17761.0
33	26973.0
34	44310.0
35	54709.0
36	49301.0
37	76504.0
38	117192.0
39	163307.0
40	50.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.620750953011125	21.51879357680381	24.679531493807897	10.180923976377175
2	31.89361169817035	25.22977608353579	25.28952444909703	17.587087769196838
3	32.037797836549544	26.242700165254433	25.301704556677002	16.417797441519024
4	29.8779684897325	25.067155187738255	26.420628493551128	18.634247828978122
5	23.03373560607557	39.83372507192847	20.24761829517997	16.884921026815988
6	27.358825969306128	35.5920849052256	22.93530716914548	14.113781956322793
7	79.85146852594363	6.502366891175676	8.848025176611559	4.798139406269134
8	80.06807692560916	7.076642503966765	7.86703272827826	4.988247842145806
9	72.75984778157446	8.791404135969504	10.319678445159889	8.129069637296148
10	44.21576566789784	24.707677418080547	17.177079012687063	13.899477901334546
11	34.0631522118417	25.346968469980975	22.670636723353546	17.919242594823785
12	33.32395135857578	21.727995154292334	26.172582248645377	18.775471238486507
13	26.753770895468342	24.496171495914727	27.69213296727172	21.05792464134521
14	20.535332187744835	27.627940508404276	27.890306609518916	23.94642069433197
15	19.9771869876948	25.22878850724552	34.951806277034905	19.842218228024784
16	24.030529275053166	24.82009651912277	30.300815738015764	20.848558467808303
17	22.950779197693024	24.953583914357385	26.86569620836543	25.22994067958416
18	25.076866354592557	26.727106335631095	26.95326130610256	21.242766003673783
19	26.36483043315096	27.927011528307226	23.931277857881188	21.776880180660623
20	27.741511781785142	26.515600413465272	26.50243272959503	19.240455075154557
21	25.797138662294994	28.119753500957952	25.77393061947369	20.309177217273366
22	23.560936749030528	25.93095524962637	25.848492629388954	24.65961537195415
23	24.02674356594047	26.422274454034905	27.608353578647282	21.94262840137734
24	25.597648251660775	23.323095459124218	27.47585375970294	23.60340252951207
25	22.490733242476317	26.989637032794118	25.717967963025146	24.801661761704423
26	21.913165708717667	28.424420786505756	25.746607675442927	23.91580582933365
27	23.158499410746145	28.1426323516825	26.48284579983804	22.216022437733315
28	20.485953373231418	28.428864879811965	28.20452046587266	22.880661281083963
29	23.124098836635127	27.849815981617915	26.51082712806231	22.515258053684647
30	25.676818950930624	26.64201017861963	25.81475043947145	21.86642043097829
31	24.824376016380597	27.963222658950404	23.919426942397966	23.29297438227103
32	26.285495137832733	27.968654328546883	24.562174511314332	21.183676022306056
33	26.553786696688984	26.568764937091387	24.39264058148492	22.484807784734702
34	22.54060584513487	27.323273222856464	27.049056206258598	23.087064725750064
35	22.730549684963165	26.574690394833	26.833435382883327	23.86132453732051
36	23.75878119918097	30.046350247223263	25.065509227254474	21.129359326341294
37	23.377741347185736	28.982236794459038	26.536833303706043	21.10318855464918
38	23.378728923476004	27.786611099040737	24.996049694838927	23.838610282644336
39	24.016867803037783	25.284751163694065	26.851705544253296	23.84667548901486
40	25.114558849671138	23.724216029021576	27.51469842712016	23.646526694187127
41	20.369913159124874	25.683073600768992	27.143863530124367	26.80314970998176
42	22.073646855886285	25.35437529215799	27.226984534555292	25.344993317400437
43	23.405887271458386	24.59855023800589	27.942812748951525	24.052749741584204
44	23.04657409784906	25.517160784003895	27.247394444554175	24.18887067359287
45	23.296101707190214	24.923298241455818	25.63287180601368	26.147728245340286
46	23.5499088137892	26.653696498054476	25.349931198851777	24.44646348930455
47	20.517226622423248	25.35733802102879	29.50614601644644	24.61928934010152
48	21.808153429852457	23.868731359497524	29.21909050807508	25.10402470257494
49	22.08154746620843	23.69195520353947	29.709422136193353	24.51707519405874
50	20.126804795670466	24.19710047601177	29.05465905574539	26.621435672572375
51	19.66511287996998	23.929631897397407	27.36754955987017	29.03770566276245
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	171.0
1	450.0
2	729.0
3	1061.0
4	1393.0
5	1004.0
6	615.0
7	585.5
8	556.0
9	558.0
10	560.0
11	586.0
12	612.0
13	615.0
14	618.0
15	642.5
16	667.0
17	934.0
18	1201.0
19	1131.5
20	1062.0
21	1357.0
22	1652.0
23	2026.5
24	2401.0
25	2686.5
26	3759.5
27	4547.0
28	5950.0
29	7353.0
30	9085.0
31	10817.0
32	11605.0
33	12393.0
34	14429.5
35	16466.0
36	17343.0
37	18220.0
38	19295.5
39	20371.0
40	22882.0
41	25393.0
42	29548.5
43	33704.0
44	37288.5
45	40873.0
46	48021.5
47	55170.0
48	54419.5
49	53669.0
50	53298.0
51	52927.0
52	46458.5
53	39990.0
54	37129.5
55	34269.0
56	33093.5
57	31918.0
58	32041.5
59	32165.0
60	31307.5
61	30450.0
62	27831.5
63	25213.0
64	20673.0
65	16133.0
66	13451.0
67	10769.0
68	8953.5
69	7138.0
70	5694.5
71	4251.0
72	3796.5
73	3342.0
74	2640.0
75	1677.0
76	1416.0
77	1109.0
78	802.0
79	546.0
80	290.0
81	252.0
82	214.0
83	140.5
84	67.0
85	50.0
86	33.0
87	22.5
88	12.0
89	10.5
90	9.0
91	7.5
92	6.0
93	6.5
94	7.0
95	4.0
96	1.0
97	1.5
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	607548.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.576250657317637
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.38173929851067	20.398224745495412
2	10.650116937834985	6.086808222906334
3	4.159289264248172	3.565706777143381
4	2.2940980173727676	2.6222687990759854
5	1.5184545437851056	2.169586882747304
6	1.0642568480927583	1.8247482272919286
7	0.8291876553861717	1.6586551997588133
8	0.6401404672902614	1.463425155934316
9	0.5009967455888951	1.2884947722403792
>10	6.285852213816538	43.67330252952261
>50	0.6162155617741504	10.582690143241951
>100	0.0538609466588307	2.6339220822859897
>500	0.0034748997844406904	0.6484264353375122
>1k	0.002316599856293793	1.3837400270181062
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGATGCCTTCGTATGC	2460	0.40490627901005355	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGATGCCTTCGTATGCCGTCTTC	2227	0.3665553997379631	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGATGCCTTCGTATGCCG	2216	0.3647448432058043	No Hit
GCTGTCTCTTATACACATCTGACGCAGATGCCTTCGTATGCCGTCTTCTGC	1458	0.23998103853522684	No Hit
CTGTCTCTTATACACATCTGACGCAGATGCCTTCGTATGCCGTCTTCTGCT	886	0.1458320988629705	No Hit
CCTGTCTCTTATACACATCTGACGCAGATGCCTTCGTATGCCGTCTTCTGC	852	0.1402358332181161	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.6459604837807055E-4	0.0	0.0	0.18138484531263374	0.0
2	1.6459604837807055E-4	0.0	0.0	0.8180423604390106	0.0
3	1.6459604837807055E-4	0.0	0.0	1.0797500773601427	0.0
4	1.6459604837807055E-4	0.0	0.0	1.498153232337198	0.0
5	1.6459604837807055E-4	0.0	0.0	2.844384312021437	0.0
6	1.6459604837807055E-4	0.0	0.0	3.5197218985166603	0.0
7	1.6459604837807055E-4	0.0	0.0	4.2413109746061215	0.0
8	1.6459604837807055E-4	0.0	0.0	5.293573511887127	0.0
9	1.6459604837807055E-4	0.0	0.0	5.663750024689407	0.0
10	1.6459604837807055E-4	0.0	0.0	6.913857012120853	0.0
11	1.6459604837807055E-4	0.0	0.0	8.168737284955263	0.0
12	1.6459604837807055E-4	0.0	0.0	9.610927860843917	0.0
13	1.6459604837807055E-4	0.0	0.0	10.07393654493143	0.0
14	1.6459604837807055E-4	0.0	0.0	10.266843113630529	0.0
15	1.6459604837807055E-4	0.0	0.0	10.666317723044106	0.0
16	1.6459604837807055E-4	0.0	0.0	11.369142849618466	0.0
17	1.6459604837807055E-4	0.0	0.0	12.142744276995398	0.0
18	1.6459604837807055E-4	0.0	0.0	12.978727606707618	0.0
19	1.6459604837807055E-4	0.0	0.0	13.499344907727455	0.0
20	1.6459604837807055E-4	0.0	0.0	14.045310000197516	0.0
21	1.6459604837807055E-4	0.0	0.0	14.656290531776913	0.0
22	1.6459604837807055E-4	0.0	0.0	15.302165425612461	0.0
23	1.6459604837807055E-4	0.0	0.0	15.925490660820214	0.0
24	1.6459604837807055E-4	0.0	0.0	16.413353348212816	0.0
25	1.6459604837807055E-4	0.0	0.0	16.850026664559838	0.0
26	1.6459604837807055E-4	0.0	0.0	17.274849065423638	0.0
27	1.6459604837807055E-4	0.0	0.0	17.68847893499773	0.0
28	1.6459604837807055E-4	0.0	0.0	18.12432927110286	0.0
29	1.6459604837807055E-4	0.0	0.0	18.539934293257488	0.0
30	1.6459604837807055E-4	0.0	0.0	19.061374574519213	0.0
31	1.6459604837807055E-4	0.0	0.0	19.530802504493472	0.0
32	1.6459604837807055E-4	0.0	0.0	19.969780165517786	0.0
33	3.291920967561411E-4	0.0	0.0	20.40628888581643	0.0
34	3.291920967561411E-4	0.0	0.0	20.84213922192156	0.0
35	3.291920967561411E-4	0.0	0.0	21.33592736705577	0.0
36	3.291920967561411E-4	0.0	0.0	21.813255907352175	0.0
37	3.291920967561411E-4	0.0	0.0	22.262109331279174	0.0
38	3.291920967561411E-4	0.0	0.0	22.78618314931495	0.0
39	3.291920967561411E-4	0.0	0.0	23.341859408639316	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATCACG	30	2.1638061E-6	45.000004	1
CGTCTAC	30	2.1638061E-6	45.000004	27
AATGCGT	30	2.1638061E-6	45.000004	23
TACGATG	35	1.2106648E-7	45.000004	1
GGTTACG	30	2.1638061E-6	45.000004	1
CTAGATC	25	3.8886268E-5	45.0	34
TCCGCGA	25	3.8886268E-5	45.0	32
CGAGTTA	20	7.0306583E-4	45.0	29
TCCGATC	25	3.8886268E-5	45.0	12
TACGGAA	20	7.0306583E-4	45.0	13
TATGCGT	20	7.0306583E-4	45.0	14
ACGGGTC	50	2.1827873E-11	45.0	5
TACGCTC	20	7.0306583E-4	45.0	10
CTAACGC	20	7.0306583E-4	45.0	17
CCGTAAT	25	3.8886268E-5	45.0	19
TCTACCG	25	3.8886268E-5	45.0	1
TCAGCGA	20	7.0306583E-4	45.0	18
TACGACG	25	3.8886268E-5	45.0	1
GTACTAA	20	7.0306583E-4	45.0	28
GTCCGCG	25	3.8886268E-5	45.0	31
>>END_MODULE