##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936763.fastq
File type	Conventional base calls
Encoding	Illumina 1.5
Total Sequences	513174
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.013695159926264	33.0	31.0	34.0	30.0	34.0
2	32.100642277278276	33.0	31.0	34.0	30.0	34.0
3	32.04562585010152	33.0	31.0	34.0	30.0	34.0
4	35.74027717694194	37.0	35.0	37.0	35.0	37.0
5	30.8704513478859	37.0	35.0	37.0	0.0	37.0
6	33.22444434051608	37.0	35.0	37.0	17.0	37.0
7	35.17605724374189	37.0	35.0	37.0	32.0	37.0
8	35.606273895403895	37.0	35.0	37.0	33.0	37.0
9	37.63228456624849	39.0	37.0	39.0	35.0	39.0
10	37.36915354246318	39.0	37.0	39.0	34.0	39.0
11	37.16608596694299	39.0	37.0	39.0	34.0	39.0
12	37.006574768012406	39.0	35.0	39.0	33.0	39.0
13	36.75212695888724	39.0	35.0	39.0	33.0	39.0
14	37.9635932451761	40.0	37.0	41.0	33.0	41.0
15	38.02768846434153	40.0	36.0	41.0	33.0	41.0
16	38.05013504191561	40.0	36.0	41.0	33.0	41.0
17	38.01351393484471	40.0	36.0	41.0	33.0	41.0
18	37.96723723337504	40.0	36.0	41.0	33.0	41.0
19	37.91448319673249	40.0	36.0	41.0	33.0	41.0
20	37.92132103341167	40.0	36.0	41.0	33.0	41.0
21	37.9013706851867	40.0	36.0	41.0	33.0	41.0
22	37.912142859926654	40.0	36.0	41.0	33.0	41.0
23	37.958413715425955	40.0	36.0	41.0	34.0	41.0
24	37.918427278077225	40.0	36.0	41.0	34.0	41.0
25	37.75451016614248	40.0	36.0	41.0	33.0	41.0
26	37.71702580411323	40.0	36.0	41.0	33.0	41.0
27	37.666577028454284	40.0	36.0	41.0	33.0	41.0
28	37.60815045189351	40.0	36.0	41.0	33.0	41.0
29	37.55426424565547	40.0	36.0	41.0	33.0	41.0
30	37.4736931333232	40.0	36.0	41.0	33.0	41.0
31	37.38970602563653	39.0	35.0	41.0	33.0	41.0
32	37.32543542735992	39.0	35.0	41.0	33.0	41.0
33	37.22425726946416	39.0	35.0	41.0	32.0	41.0
34	37.124986846566664	39.0	35.0	41.0	32.0	41.0
35	37.08079325920643	39.0	35.0	41.0	32.0	41.0
36	36.92785682828826	39.0	35.0	41.0	31.0	41.0
37	36.88008355840319	39.0	35.0	41.0	31.0	41.0
38	36.77139137992182	39.0	35.0	41.0	31.0	41.0
39	36.58839886666121	39.0	35.0	41.0	30.0	41.0
40	36.37200053003465	39.0	35.0	41.0	30.0	41.0
41	36.35488158012682	39.0	35.0	41.0	30.0	41.0
42	36.30366893100586	39.0	35.0	41.0	30.0	41.0
43	36.19950932822006	39.0	35.0	40.0	30.0	41.0
44	36.039655165694285	38.0	35.0	40.0	30.0	41.0
45	36.01707997677201	38.0	35.0	40.0	30.0	41.0
46	35.73600572125634	38.0	35.0	40.0	29.0	41.0
47	35.59703726221515	38.0	35.0	40.0	28.0	41.0
48	35.51757493559689	38.0	35.0	40.0	27.0	41.0
49	35.38698570075647	38.0	34.0	40.0	27.0	41.0
50	35.21646459095745	38.0	34.0	40.0	26.0	41.0
51	33.372312315121185	36.0	32.0	39.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	15.0
9	22.0
10	34.0
11	25.0
12	18.0
13	8.0
14	17.0
15	17.0
16	24.0
17	54.0
18	108.0
19	239.0
20	429.0
21	659.0
22	1011.0
23	1513.0
24	2084.0
25	2875.0
26	4032.0
27	4922.0
28	6120.0
29	7595.0
30	9856.0
31	12558.0
32	16388.0
33	24018.0
34	38294.0
35	46924.0
36	41795.0
37	62869.0
38	97115.0
39	131479.0
40	56.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.68070089287454	21.477510551976522	25.07628991336272	9.765498641786216
2	29.523904172853655	27.586159860008497	25.55390569280595	17.336030274331904
3	31.42852132025395	27.438451675260247	25.793395612404368	15.339631392081438
4	28.600045988300266	25.046085733104174	28.1662749866517	18.187593291943863
5	23.89053225611586	39.02925713305818	20.101369126261268	16.97884148456469
6	25.859260211935915	35.89464781925818	23.96243769169911	14.283654277106791
7	78.05188883302739	6.628161208478995	11.171064785043669	4.1488851734499415
8	78.96035262893287	7.623535097257459	8.16740520759041	5.2487070662192545
9	72.03813911071099	8.46165238301239	10.193033941703982	9.307174564572641
10	41.077295420266815	27.675603206709614	17.493286877355438	13.753814495668134
11	31.652616851204463	25.57241793231925	24.729623870266227	18.04534134621006
12	30.361436861571317	20.796844734924218	29.245830848795922	19.59588755470854
13	24.881229368596227	26.334537603230096	30.182745033848168	18.60148799432551
14	19.47039405737625	30.200088079286946	27.831300884300454	22.49821697903635
15	18.494701602185614	26.30238476618067	36.51393094739796	18.688982684235754
16	20.53124281432808	26.58571946357376	31.701528136655405	21.181509585442754
17	20.92370229201012	25.550787841940547	28.769773994785396	24.755735871263937
18	21.755194144676075	27.29444593841465	28.97438295782717	21.975976959082104
19	23.847466941037542	28.89780074594582	25.62834438221734	21.6263879307993
20	24.901495399221314	27.352126179424523	28.284363588178667	19.46201483317549
21	23.582839348836846	29.12150654553816	27.607789950387197	19.687864155237794
22	20.513899768889303	26.026260098913816	28.136070806393153	25.323769325803724
23	20.481746931839883	27.997911039920183	28.501054223323862	23.019287804916072
24	23.281771874646807	24.429140993113446	28.653634050049305	23.63545308219045
25	20.267784416201913	29.002833346973933	27.051448436592658	23.6779338002315
26	19.20167428591472	29.134562546037017	27.631173831877685	24.032589336170577
27	20.63724974375163	29.158531024564766	26.81858395008321	23.385635281600393
28	17.589550522824617	28.266046214344453	30.62255687154844	23.521846391282487
29	20.20464793617759	25.33000502753452	30.844314014349912	23.62103302193798
30	20.68849941735162	27.67151102744878	28.693191782904044	22.946797772295557
31	23.110679808408065	29.957480308823126	24.565352102795543	22.366487779973266
32	25.306036549006773	29.010238242779252	24.313975376772788	21.369749831441187
33	22.734589047769372	28.707806708835598	24.057727008772854	24.499877234622176
34	20.70194514920865	29.59230202621333	27.515423618499767	22.19032920607825
35	19.887991207660562	26.484389310448307	26.23866368911909	27.38895579277204
36	20.179120532217144	29.50091002272134	26.881525564428436	23.43844388063308
37	20.836012736420788	29.306239209313024	27.094124020312798	22.763624033953395
38	19.94859443385674	30.01262729600486	24.525404638582625	25.51337363155577
39	22.966479205883385	27.61383078643891	25.850296390697892	23.569393616979816
40	21.541621360396277	26.85755708590069	28.360945800060016	23.239875753643013
41	17.842096442921893	27.691387326715695	26.92907279012577	27.537443440236647
42	20.093574499097773	25.16281027487753	28.01642327943349	26.727191946591216
43	22.35635476466072	24.984508178512552	25.568130887379333	27.0910061694474
44	21.01450969846485	26.366105843242256	26.35597282792971	26.263411630363194
45	20.541765560998805	26.272180585922122	26.033859860398227	27.152193992680846
46	22.97057138514422	27.743416463032034	25.078043704474506	24.20796844734924
47	19.09800574464022	25.63321602419452	30.041077685151624	25.227700546013633
48	20.3422231056133	24.429530724471622	28.548016851983927	26.680229317931147
49	19.99848004770312	23.08417807605218	31.314914629345992	25.602427246898714
50	19.358151426221905	23.894429569697607	30.305900143031412	26.44151886104908
51	18.87118209418248	23.825252253621578	27.477229945398634	29.826335706797302
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	99.0
1	317.5
2	536.0
3	838.0
4	1140.0
5	812.0
6	484.0
7	459.0
8	434.0
9	407.0
10	380.0
11	412.5
12	445.0
13	452.5
14	460.0
15	579.0
16	698.0
17	680.0
18	662.0
19	880.0
20	1098.0
21	1192.0
22	1286.0
23	1602.5
24	1919.0
25	2506.5
26	3931.5
27	4769.0
28	5645.0
29	6521.0
30	8102.0
31	9683.0
32	10248.0
33	10813.0
34	12623.5
35	14434.0
36	15320.5
37	16207.0
38	17151.0
39	18095.0
40	21997.0
41	25899.0
42	29197.5
43	32496.0
44	38553.0
45	44610.0
46	54019.5
47	63429.0
48	58331.5
49	53234.0
50	50244.0
51	47254.0
52	41120.0
53	34986.0
54	29621.5
55	24257.0
56	22372.0
57	20487.0
58	20680.5
59	20874.0
60	19193.0
61	17512.0
62	15487.5
63	13463.0
64	11662.5
65	9862.0
66	7484.0
67	5106.0
68	3873.0
69	2640.0
70	2214.0
71	1788.0
72	1307.5
73	827.0
74	660.5
75	476.0
76	458.0
77	294.0
78	130.0
79	88.5
80	47.0
81	38.5
82	30.0
83	27.5
84	25.0
85	15.0
86	5.0
87	3.0
88	1.0
89	1.0
90	1.0
91	1.0
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	513174.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.804897109889733
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.29436520382477	22.27020481728615
2	11.016098756552914	6.786995774959935
3	4.384926557573666	4.052316343214392
4	2.367871811235941	2.917681900581257
5	1.4713026126528943	2.266166280014218
6	0.9860784697362543	1.8225627481501705
7	0.7182332323591902	1.5487570576609866
8	0.5775182451386134	1.423231209646325
9	0.44697616950786717	1.2392149421036236
>10	5.076992800896272	37.89784514456609
>50	0.6004788010148709	11.1952159992784
>100	0.049615833134703316	2.681380913554787
>500	0.006361004248038887	1.2060727508718
>1k	0.0031805021240194436	2.6923541181118655
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGC	4006	0.7806319104241446	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTC	3540	0.6898245039694139	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCG	3349	0.6526051592637195	No Hit
GCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGC	1702	0.3316613858067634	RNA PCR Primer, Index 40 (96% over 27bp)
GAATGCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTT	1143	0.22273147119690398	No Hit
CTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGCT	724	0.14108275165928125	Illumina PCR Primer Index 8 (95% over 24bp)
CCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCTGC	698	0.13601624400300874	Illumina PCR Primer Index 8 (95% over 23bp)
GAATGACTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCT	685	0.13348299017487245	No Hit
GAATATGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTC	652	0.1270524227649881	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGC	638	0.12432430325776442	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTC	593	0.1155553476988312	RNA PCR Primer, Index 40 (95% over 21bp)
GAATAATACCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCG	573	0.1116580341170831	No Hit
GAACTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCT	540	0.10522746670719872	RNA PCR Primer, Index 40 (96% over 25bp)
GCTTGTGGGATTGTGATGATCTAGTGGGCATGTCTGTCACCTGGACAATTG	540	0.10522746670719872	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.897313581748101E-4	0.0	0.0	0.21980848601059289	0.0
2	3.897313581748101E-4	0.0	0.0	1.1497075066156899	0.0
3	3.897313581748101E-4	0.0	0.0	1.5256034015752942	0.0
4	3.897313581748101E-4	0.0	0.0	2.12442563341089	0.0
5	3.897313581748101E-4	0.0	0.0	4.390908346876498	0.0
6	3.897313581748101E-4	0.0	0.0	5.577640332518794	0.0
7	3.897313581748101E-4	0.0	0.0	6.565999056850114	0.0
8	3.897313581748101E-4	0.0	0.0	7.949545378370689	0.0
9	3.897313581748101E-4	0.0	0.0	8.364609274826861	0.0
10	3.897313581748101E-4	0.0	0.0	10.222263793567095	0.0
11	3.897313581748101E-4	0.0	0.0	11.688628028699817	0.0
12	3.897313581748101E-4	0.0	0.0	13.91945032289243	0.0
13	3.897313581748101E-4	0.0	0.0	14.643766052060315	0.0
14	3.897313581748101E-4	0.0	0.0	14.983222065030574	0.0
15	3.897313581748101E-4	0.0	0.0	15.485585785717905	0.0
16	3.897313581748101E-4	0.0	0.0	16.318441698137473	0.0
17	3.897313581748101E-4	0.0	0.0	17.27386812270302	0.0
18	3.897313581748101E-4	0.0	0.0	18.3201019537233	0.0
19	3.897313581748101E-4	0.0	0.0	18.939385081863072	0.0
20	3.897313581748101E-4	0.0	0.0	19.588092927545045	0.0
21	3.897313581748101E-4	0.0	0.0	20.376324599453596	0.0
22	3.897313581748101E-4	0.0	0.0	21.19242206347165	0.0
23	3.897313581748101E-4	0.0	0.0	21.99526866131176	0.0
24	3.897313581748101E-4	0.0	0.0	22.587270594379294	0.0
25	3.897313581748101E-4	0.0	0.0	23.158616765463567	0.0
26	3.897313581748101E-4	0.0	0.0	23.658836963680933	0.0
27	3.897313581748101E-4	0.0	0.0	24.155744445353818	0.0
28	3.897313581748101E-4	0.0	0.0	24.703901600626686	0.0
29	3.897313581748101E-4	0.0	0.0	25.244653860094235	0.0
30	3.897313581748101E-4	0.0	0.0	25.85049125637698	0.0
31	3.897313581748101E-4	0.0	0.0	26.399038143008024	0.0
32	3.897313581748101E-4	0.0	0.0	26.920498700245922	0.0
33	3.897313581748101E-4	0.0	0.0	27.43923113797659	0.0
34	3.897313581748101E-4	0.0	0.0	27.96497874015441	0.0
35	3.897313581748101E-4	0.0	0.0	28.53963762778317	0.0
36	3.897313581748101E-4	0.0	0.0	29.080389887250718	0.0
37	3.897313581748101E-4	0.0	0.0	29.603993966958576	0.0
38	3.897313581748101E-4	0.0	0.0	30.136756733583542	0.0
39	3.897313581748101E-4	0.0	0.0	30.73246111455374	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTGC	20	7.0296315E-4	45.000004	20
ACTGCGC	20	7.0296315E-4	45.000004	25
GATCGGT	20	7.0296315E-4	45.000004	10
TAGCGTT	20	7.0296315E-4	45.000004	1
GACGTAG	20	7.0296315E-4	45.000004	1
GGCCGAA	20	7.0296315E-4	45.000004	7
GTCTCGC	20	7.0296315E-4	45.000004	31
ACCGAAT	40	6.8030204E-9	45.000004	28
TATTGCG	30	2.163144E-6	45.000004	1
ACGTATG	20	7.0296315E-4	45.000004	1
TAGTGCG	30	2.163144E-6	45.000004	1
GCGTCAG	20	7.0296315E-4	45.000004	1
ATCGCGG	20	7.0296315E-4	45.000004	2
GCGTAAG	60	0.0	45.000004	1
CGGGTAC	25	3.8877777E-5	45.0	6
TAGCGAG	45	3.8380676E-10	45.0	1
CGTCTTA	25	3.8877777E-5	45.0	26
ATTCGTC	50	2.1827873E-11	45.0	37
TTCGTCA	45	3.8380676E-10	45.0	38
TACGGAC	25	3.8877777E-5	45.0	24
>>END_MODULE