##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936761.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	756448
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.07698612462456	33.0	31.0	34.0	30.0	34.0
2	32.17320423875798	33.0	31.0	34.0	30.0	34.0
3	32.10568472651127	33.0	31.0	34.0	30.0	34.0
4	35.79051699521976	37.0	35.0	37.0	35.0	37.0
5	31.003913025085662	37.0	35.0	37.0	0.0	37.0
6	33.34507461187022	37.0	35.0	37.0	17.0	37.0
7	35.29166446338677	37.0	35.0	37.0	32.0	37.0
8	35.72039849401413	37.0	35.0	37.0	35.0	37.0
9	37.710075246414824	39.0	37.0	39.0	35.0	39.0
10	37.436856201615974	39.0	37.0	39.0	35.0	39.0
11	37.256593976056514	39.0	37.0	39.0	34.0	39.0
12	37.20660772452303	39.0	37.0	39.0	34.0	39.0
13	37.107317356910194	39.0	37.0	39.0	33.0	39.0
14	38.317426974491305	40.0	38.0	41.0	34.0	41.0
15	38.405503088117094	40.0	38.0	41.0	34.0	41.0
16	38.38644824231143	40.0	38.0	41.0	34.0	41.0
17	38.37061899826558	40.0	37.0	41.0	34.0	41.0
18	38.3148055120775	40.0	37.0	41.0	34.0	41.0
19	38.28643475823851	40.0	37.0	41.0	34.0	41.0
20	38.272009444138924	40.0	37.0	41.0	34.0	41.0
21	38.21634930623123	40.0	37.0	41.0	34.0	41.0
22	38.20875195651254	40.0	37.0	41.0	34.0	41.0
23	38.20782790092643	40.0	37.0	41.0	34.0	41.0
24	38.182250465332714	40.0	37.0	41.0	34.0	41.0
25	38.04357867295571	40.0	37.0	41.0	34.0	41.0
26	38.01215285122044	40.0	37.0	41.0	34.0	41.0
27	37.963619442446806	40.0	37.0	41.0	34.0	41.0
28	37.90688851051229	40.0	37.0	41.0	33.0	41.0
29	37.9012886543424	40.0	37.0	41.0	34.0	41.0
30	37.83158525106815	40.0	36.0	41.0	33.0	41.0
31	37.75063718854435	40.0	36.0	41.0	33.0	41.0
32	37.67961578323956	40.0	36.0	41.0	33.0	41.0
33	37.594896146199076	40.0	36.0	41.0	33.0	41.0
34	37.49880494098735	40.0	36.0	41.0	33.0	41.0
35	37.41116243284403	40.0	36.0	41.0	33.0	41.0
36	37.28809514996404	40.0	35.0	41.0	32.0	41.0
37	37.25586821566056	40.0	35.0	41.0	32.0	41.0
38	37.19202906214307	39.0	35.0	41.0	32.0	41.0
39	37.12748133381277	39.0	35.0	41.0	32.0	41.0
40	36.99984400778375	39.0	35.0	41.0	31.0	41.0
41	36.91946042556792	39.0	35.0	41.0	31.0	41.0
42	36.83800869326114	39.0	35.0	41.0	31.0	41.0
43	36.79659408181395	39.0	35.0	41.0	31.0	41.0
44	36.666089407335335	39.0	35.0	41.0	31.0	41.0
45	36.616928063792884	39.0	35.0	41.0	31.0	41.0
46	36.43364778543932	39.0	35.0	40.0	30.0	41.0
47	36.33354969541859	39.0	35.0	40.0	30.0	41.0
48	36.20522098015991	39.0	35.0	40.0	30.0	41.0
49	36.07894924700707	38.0	35.0	40.0	29.0	41.0
50	35.92389562798765	38.0	35.0	40.0	29.0	41.0
51	34.22678359913702	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	29.0
9	38.0
10	54.0
11	46.0
12	23.0
13	20.0
14	18.0
15	19.0
16	52.0
17	88.0
18	150.0
19	319.0
20	498.0
21	888.0
22	1303.0
23	1817.0
24	2698.0
25	3556.0
26	4766.0
27	5876.0
28	7009.0
29	8838.0
30	11372.0
31	15251.0
32	20302.0
33	30295.0
34	47831.0
35	60504.0
36	63003.0
37	97289.0
38	153705.0
39	218730.0
40	60.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.57384512881256	22.281901730191635	24.840835060704766	10.303418080291046
2	31.34848661110876	25.23226976606455	25.695883920639623	17.723359702187064
3	32.65816024366513	25.01771437032023	26.142312492068193	16.181812893946447
4	28.942372773805996	25.652391175599643	26.559525572147724	18.845710478446634
5	22.467638224967214	39.79255679174246	20.94644972291552	16.793355260374803
6	26.66409323575447	34.9832374465925	24.023197893311902	14.329471424341131
7	80.1875343711663	6.423706586573036	8.772843605905495	4.615915436355176
8	80.73165637294302	5.9160708997842555	8.197919751258514	5.154352976014214
9	73.61550298235966	8.460198189432717	10.758968230466602	7.165330597741021
10	39.49815453276365	28.506916536232495	17.714370320233513	14.280558610770337
11	29.360511231439567	26.006678582004316	25.792387579846864	18.84042260670925
12	28.28257857777402	22.499365455391516	29.526286010406533	19.691769956427937
13	24.405643216718133	24.77632302550869	30.317748212699353	20.50028554507382
14	19.396442319895087	28.680755319598966	29.291107914886418	22.631694445619527
15	18.622562291129068	27.35984495960066	33.99070392148568	20.026888827784592
16	21.616158678455093	26.61544481577055	30.731656372943018	21.036740132831337
17	20.519850670502134	26.41424129616312	28.59191907441093	24.47398895892381
18	22.41436291721308	27.554306442742927	29.135512289013914	20.895818351030076
19	23.984067642455265	28.30425885189729	26.745658657303608	20.96601484834384
20	24.668450442066078	28.037485722746307	27.945608951309275	19.348454883878336
21	23.93370066415669	28.049383434155423	28.238557045560302	19.778358856127586
22	21.537501586361522	26.47478742755616	27.067822242903677	24.919888743178646
23	21.450516096281568	27.768200854520074	28.195328694107197	22.58595435509116
24	22.21514234950717	25.48886374212107	29.219986040018615	23.076007868353145
25	21.234109945429164	28.465803333474344	26.865561148948768	23.43452557214772
26	20.13568678878125	28.849041837641188	28.011046364059393	23.00422500951817
27	22.107534159651422	28.47532150260163	27.959886205000213	21.45725813274673
28	19.46663881720885	28.7970884978214	29.09902597402597	22.637246710943778
29	21.86495304369897	26.561508524049245	29.185086086551888	22.388452345699903
30	23.942557849316806	26.338360336731668	28.273589195820463	21.445492618131055
31	23.43360019459368	29.43573120690385	25.717564194762893	21.413104403739585
32	25.592638224967217	28.64056749439486	25.26796290029189	20.49883138034604
33	25.072047252421847	28.36176445704133	24.440675366978297	22.125512923558528
34	22.974084140615087	27.27008333685858	27.798209526629726	21.95762299589661
35	23.759200896823046	26.57803312322856	27.36116692753501	22.301599052413383
36	23.86323977325606	30.320788738948345	25.661777147933503	20.15419433986209
37	24.361357290917553	29.32257075172385	27.00066627183891	19.315405685519693
38	23.105752146875925	29.965047167815897	25.02657155548035	21.902629129827826
39	25.44788273615635	26.315886881847796	26.202197639494056	22.034032742501797
40	24.91367549388722	25.252760269046913	29.28000338423791	20.553560852827953
41	21.482111129912433	28.55411079148864	27.28211324506113	22.6816648335378
42	21.736589957273996	28.885792546216	27.82134396548077	21.556273531029234
43	23.69257371293202	27.056056728288	26.88340771606244	22.36796184271754
44	23.045866999450062	26.536523330090105	26.52568319302847	23.89192647743136
45	23.467178180126062	25.036618511781377	26.3484072930327	25.14779601505986
46	24.227574135961756	26.961800414569144	26.441738229197515	22.368887220271585
47	22.23854118194509	26.73217458437328	29.31463894411777	21.714645289563855
48	22.0623228562968	26.008397140318962	28.57631985278565	23.352960150598587
49	22.963376200346886	25.644062777613264	29.044825288717796	22.347735733322054
50	21.820667117898388	24.440146579804562	29.02102986589957	24.71815643639748
51	21.40702335124159	24.334653538643767	27.10893544566183	27.149387664452814
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	203.0
1	519.0
2	835.0
3	1585.0
4	2335.0
5	1621.5
6	908.0
7	805.5
8	703.0
9	721.5
10	740.0
11	745.5
12	751.0
13	849.5
14	948.0
15	1009.5
16	1071.0
17	1205.5
18	1340.0
19	1496.5
20	1653.0
21	1890.5
22	2128.0
23	2538.0
24	2948.0
25	4046.0
26	5899.0
27	6654.0
28	9023.0
29	11392.0
30	13135.0
31	14878.0
32	16531.5
33	18185.0
34	20981.0
35	23777.0
36	25081.5
37	26386.0
38	28675.5
39	30965.0
40	35295.5
41	39626.0
42	43597.5
43	47569.0
44	51921.5
45	56274.0
46	64274.0
47	72274.0
48	75692.0
49	79110.0
50	75467.0
51	71824.0
52	60916.0
53	50008.0
54	43561.5
55	37115.0
56	34661.5
57	32208.0
58	30907.5
59	29607.0
60	27810.5
61	26014.0
62	24384.5
63	22755.0
64	18512.0
65	14269.0
66	11033.0
67	7797.0
68	6613.5
69	5430.0
70	4728.0
71	4026.0
72	3315.0
73	2604.0
74	2299.0
75	1420.0
76	846.0
77	676.0
78	506.0
79	390.0
80	274.0
81	248.0
82	222.0
83	145.0
84	68.0
85	53.5
86	39.0
87	32.5
88	26.0
89	20.0
90	14.0
91	8.5
92	3.0
93	2.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	756448.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.21257155820819
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.7547477487383	23.43617518055618
2	10.891192574173884	7.016666402996037
3	4.132308303812468	3.9933683081141105
4	2.1559179735250558	2.7779064798321222
5	1.430218021232584	2.3035500176396764
6	0.982623895776707	1.8991705534507488
7	0.7483113639846526	1.6873523352123196
8	0.5840866121915442	1.505194543312921
9	0.4846034226579071	1.4049290186748398
>10	5.378360322899887	41.48104859694753
>50	0.39802952815248077	7.676956195375535
>100	0.05459881471287056	3.002391895916828
>500	0.002083924225682082	0.4203590317093229
>1k	0.002917493915954915	1.394931440261854
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTCTGC	1841	0.2433742967130589	No Hit
CCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTCTGC	1687	0.22301599052413382	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTC	1624	0.2146875925377554	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGC	1559	0.2060948009645078	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCG	1404	0.18560429798214814	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1147	0.1516297220694615	No Hit
CTGTCTCTTATACACATCTGACGCTGAGGGAATCGTATGCCGTCTTCTGCT	1128	0.1491179829942045	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	802	0.10602182833453191	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.18996679216548923	0.0
2	0.0	0.0	0.0	0.9693990862557638	0.0
3	0.0	0.0	0.0	1.3226289183129574	0.0
4	0.0	0.0	0.0	1.8269596852658743	0.0
5	0.0	0.0	0.0	3.3773636786666104	0.0
6	0.0	0.0	0.0	4.283969288040949	0.0
7	0.0	0.0	0.0	5.219129404797157	0.0
8	0.0	0.0	0.0	6.662982782689623	0.0
9	0.0	0.0	0.0	7.163612039426372	0.0
10	0.0	0.0	0.0	8.493247387791362	0.0
11	0.0	0.0	0.0	10.099041837641186	0.0
12	0.0	0.0	0.0	11.567616015905918	0.0
13	1.3219679343457846E-4	0.0	0.0	12.158535682558483	0.0
14	1.3219679343457846E-4	0.0	0.0	12.385253183298786	0.0
15	1.3219679343457846E-4	0.0	0.0	12.79942573712932	0.0
16	1.3219679343457846E-4	0.0	0.0	13.643502263209104	0.0
17	1.3219679343457846E-4	0.0	0.0	14.688253521722578	0.0
18	1.3219679343457846E-4	0.0	0.0	15.797913405812428	0.0
19	1.3219679343457846E-4	0.0	0.0	16.45281632048733	0.0
20	1.3219679343457846E-4	0.0	0.0	17.076917382291974	0.0
21	1.3219679343457846E-4	0.0	0.0	17.840353864376667	0.0
22	1.3219679343457846E-4	0.0	0.0	18.68509137442362	0.0
23	1.3219679343457846E-4	0.0	0.0	19.495986505351325	0.0
24	1.3219679343457846E-4	0.0	0.0	20.152475781547444	0.0
25	1.3219679343457846E-4	0.0	0.0	20.728589407335335	0.0
26	1.3219679343457846E-4	0.0	0.0	21.246007656838277	0.0
27	1.3219679343457846E-4	0.0	0.0	21.799119040568552	0.0
28	2.643935868691569E-4	0.0	0.0	22.358179280003384	0.0
29	2.643935868691569E-4	0.0	0.0	22.930855789161978	0.0
30	2.643935868691569E-4	0.0	0.0	23.57068826938534	0.0
31	2.643935868691569E-4	0.0	0.0	24.159096196962647	0.0
32	2.643935868691569E-4	0.0	0.0	24.711678793519184	0.0
33	2.643935868691569E-4	0.0	0.0	25.260295486272685	0.0
34	2.643935868691569E-4	0.0	0.0	25.815786412284783	0.0
35	3.9659038030373533E-4	0.0	0.0	26.424552646051016	0.0
36	3.9659038030373533E-4	0.0	0.0	26.985860230974236	0.0
37	3.9659038030373533E-4	0.0	0.0	27.55073712932019	0.0
38	5.287871737383138E-4	0.0	0.0	28.090100046533273	0.0
39	5.287871737383138E-4	0.0	0.0	28.668064427429247	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATACG	30	2.1645137E-6	45.000004	4
AATACGG	30	2.1645137E-6	45.000004	5
GCGAACG	20	7.031756E-4	45.0	1
TAAACGG	50	2.1827873E-11	45.0	2
TACGGGT	45	3.8380676E-10	45.0	4
TCCACCG	20	7.031756E-4	45.0	42
CATCGTT	45	3.8380676E-10	45.0	36
ACGTAAG	25	3.8895363E-5	45.0	1
CGCACGG	35	1.2111923E-7	45.0	2
GGTTACG	25	3.8895363E-5	45.0	1
AATACCG	20	7.031756E-4	45.0	1
ATACCGG	25	3.8895363E-5	45.0	2
CTTACGT	20	7.031756E-4	45.0	22
ACGCGTC	20	7.031756E-4	45.0	27
TCACGAC	85	0.0	42.35294	25
CGGTCTA	85	0.0	42.35294	31
AACGGGC	125	0.0	41.4	4
TAACGCC	60	3.6379788E-12	41.250004	12
TACGGGA	365	0.0	40.684933	4
ACGGGTA	90	0.0	40.0	5
>>END_MODULE