##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936759.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	286976
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.947988681980373	33.0	31.0	34.0	30.0	34.0
2	32.04619201605709	33.0	31.0	34.0	30.0	34.0
3	32.004728618421055	33.0	31.0	34.0	30.0	34.0
4	35.68440566458519	37.0	35.0	37.0	33.0	37.0
5	30.9136269235058	37.0	35.0	37.0	0.0	37.0
6	33.23370943911686	37.0	35.0	37.0	17.0	37.0
7	35.13624135816236	37.0	35.0	37.0	32.0	37.0
8	35.53950853033006	37.0	35.0	37.0	33.0	37.0
9	37.578884645405886	39.0	37.0	39.0	35.0	39.0
10	37.357928886039254	39.0	37.0	39.0	34.0	39.0
11	37.121888241525426	39.0	37.0	39.0	34.0	39.0
12	36.911156333630686	39.0	35.0	39.0	33.0	39.0
13	36.6350809823818	39.0	35.0	39.0	33.0	39.0
14	37.80903281110616	40.0	36.0	41.0	33.0	41.0
15	37.89825281556646	40.0	36.0	41.0	33.0	41.0
16	37.953794045495094	40.0	36.0	41.0	33.0	41.0
17	37.90762990633363	40.0	36.0	41.0	33.0	41.0
18	37.870926488626225	40.0	36.0	41.0	33.0	41.0
19	37.83408368644068	40.0	36.0	41.0	33.0	41.0
20	37.82086306868867	40.0	36.0	41.0	33.0	41.0
21	37.81259059991079	39.0	36.0	41.0	33.0	41.0
22	37.819737539027656	40.0	36.0	41.0	33.0	41.0
23	37.85528406556646	39.0	36.0	41.0	34.0	41.0
24	37.813412968331846	40.0	35.0	41.0	34.0	41.0
25	37.661870679081176	39.0	35.0	41.0	33.0	41.0
26	37.563134199375554	39.0	35.0	41.0	33.0	41.0
27	37.555001115075825	39.0	35.0	41.0	33.0	41.0
28	37.56374400646744	39.0	36.0	41.0	33.0	41.0
29	37.54840126003568	39.0	36.0	41.0	33.0	41.0
30	37.49436886708296	39.0	36.0	41.0	33.0	41.0
31	37.435583463425516	39.0	35.0	41.0	33.0	41.0
32	37.33733831400535	39.0	35.0	41.0	33.0	41.0
33	37.20925791703836	39.0	35.0	41.0	32.0	41.0
34	37.16558527542373	39.0	35.0	41.0	32.0	41.0
35	37.147977531222125	39.0	35.0	41.0	32.0	41.0
36	36.943650340098124	39.0	35.0	41.0	32.0	41.0
37	36.935576494201605	39.0	35.0	41.0	32.0	41.0
38	36.8961237176628	39.0	35.0	41.0	31.0	41.0
39	36.771907755352366	39.0	35.0	41.0	31.0	41.0
40	36.60466728925067	39.0	35.0	41.0	31.0	41.0
41	36.585261485281	39.0	35.0	40.0	31.0	41.0
42	36.535832264719	39.0	35.0	40.0	31.0	41.0
43	36.39277849018733	39.0	35.0	40.0	30.0	41.0
44	36.27365354594112	38.0	35.0	40.0	30.0	41.0
45	36.23369201605709	38.0	35.0	40.0	30.0	41.0
46	36.02222485504014	38.0	35.0	40.0	30.0	41.0
47	35.84995260927743	38.0	35.0	40.0	29.0	41.0
48	35.74810088648528	38.0	35.0	40.0	29.0	41.0
49	35.61784260704728	38.0	35.0	40.0	28.0	41.0
50	35.40381077163247	38.0	34.0	40.0	27.0	41.0
51	33.45732395740411	36.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	16.0
10	17.0
11	11.0
12	11.0
13	5.0
14	5.0
15	7.0
16	13.0
17	46.0
18	73.0
19	123.0
20	214.0
21	353.0
22	523.0
23	790.0
24	1041.0
25	1497.0
26	2043.0
27	2349.0
28	3149.0
29	4000.0
30	5167.0
31	6883.0
32	9192.0
33	13756.0
34	22232.0
35	29117.0
36	23583.0
37	34711.0
38	53662.0
39	72370.0
40	16.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.33584690008921	20.858887154326496	23.227726360392506	10.577539585191793
2	29.129962087421944	28.55116804192685	25.405260370205173	16.913609500446032
3	30.291383251561104	28.591589540588757	26.084062778768956	15.032964429081177
4	27.800234165923282	24.087380129348794	30.711975914362178	17.400409790365746
5	23.276162466547724	38.189604705619985	20.826480263157894	17.707752564674397
6	23.74937276984835	36.22672279214987	24.949821587867977	15.074082850133811
7	74.8198455619982	6.521799732381801	13.686858831400537	4.971495874219447
8	73.78247658340767	8.59375	10.835400869759143	6.788372546833185
9	68.92248829170383	7.018356935771633	12.371069357716324	11.688085414808207
10	42.2739183764496	21.619577943800177	20.740410347903655	15.366093331846564
11	32.1608775646744	24.106893956289028	23.735085860838538	19.997142618198037
12	29.097555196253346	20.367208407671722	29.43556255575379	21.09967384032114
13	21.799732381801963	27.398109946476364	31.606824264049955	19.195333407671722
14	17.96143231489741	30.947187221231044	28.342788247100803	22.748592216770742
15	16.25257861284567	24.431659790365746	40.22217885816236	19.09358273862623
16	18.553816347011598	25.180154438001782	31.965739295272076	24.30028991971454
17	18.03704839429081	25.082236842105267	29.80702219000892	27.073692573595004
18	19.266419491525426	26.786212087421944	32.03125	21.916118421052634
19	21.99487065120428	27.511011373773414	27.475468331846564	23.01864964317574
20	23.14130798394291	27.273709299732378	29.862079058876002	19.722903657448708
21	21.28888826940232	29.071072145405886	29.723739964317573	19.91629962087422
22	19.215892618198037	25.057496097234612	27.503345227475467	28.223266057091884
23	18.044017618198037	28.41561663693131	29.97254125780553	23.56782448706512
24	22.768106043710972	22.46599018733274	29.215683541480818	25.550220227475467
25	17.65025646743979	29.830020628902766	28.591589540588757	23.92813336306869
26	17.454769736842106	28.665114852809992	28.223962979482604	25.656152430865298
27	21.254390611061552	29.551251672613738	26.577135370205173	22.617222346119537
28	16.30589317573595	25.934224464763606	33.197201159678855	24.562681199821586
29	19.318688670829616	26.465976248884925	30.494187667261375	23.721147413024084
30	23.019346565566458	28.333031333630686	27.830550289919714	20.81707181088314
31	24.1971454058876	26.424857827832295	23.761917372881356	25.61607939339875
32	25.62026092774309	28.17343610615522	26.05235280999108	20.153950156110614
33	23.517297613737735	28.067503902765388	23.775158898305087	24.640039585191793
34	23.479663804638715	26.61128456735058	26.498383140053523	23.41066848795718
35	23.42181924620874	25.282950490633365	26.192782671721677	25.102447591436217
36	23.73334355486173	30.449933095450493	24.115257024977698	21.70146632471008
37	23.43157615967886	25.766614629794827	30.44644848349688	20.355360727029435
38	19.928147301516503	28.967230709188225	25.42825880909902	25.676363180196255
39	23.727071253345226	25.920286016949152	29.933513603925064	20.419129125780554
40	25.33068967439786	24.762001003568244	28.64107103033006	21.266238291703836
41	22.511638603925064	26.772970561998218	24.63341882247993	26.08197201159679
42	23.786309656556647	23.639607493309544	28.97977531222123	23.594307537912577
43	23.538205285459412	25.184335972346116	26.4436747323818	24.833784009812668
44	20.655385816235505	24.355695249776986	29.488528657448704	25.500390276538802
45	20.308666926851025	24.9027793264942	25.859305307760927	28.929248438893847
46	25.121264495985727	27.0458156779661	26.509533898305083	21.323385927743086
47	19.766112845673504	23.701633586083855	33.36655330062444	23.1657002676182
48	22.01055140499554	23.727071253345226	29.091979817127566	25.17039752453167
49	20.61879739072257	21.19410682426405	33.78470673505799	24.402389049955396
50	21.203863737734167	22.206386596788583	30.750306645851914	25.839443019625335
51	20.319120762711865	22.660779995539695	26.65449375557538	30.36560548617306
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	76.0
1	157.5
2	239.0
3	388.5
4	538.0
5	402.0
6	266.0
7	272.0
8	278.0
9	297.0
10	316.0
11	311.0
12	306.0
13	299.0
14	292.0
15	308.5
16	325.0
17	369.5
18	414.0
19	439.0
20	464.0
21	588.0
22	712.0
23	891.0
24	1070.0
25	1365.5
26	2067.5
27	2474.0
28	2628.5
29	2783.0
30	3537.0
31	4291.0
32	5158.5
33	6026.0
34	6809.0
35	7592.0
36	8359.0
37	9126.0
38	9300.0
39	9474.0
40	11086.5
41	12699.0
42	15614.5
43	18530.0
44	21698.0
45	24866.0
46	32644.5
47	40423.0
48	36547.5
49	32672.0
50	32062.5
51	31453.0
52	25664.5
53	19876.0
54	17069.0
55	14262.0
56	12145.5
57	10029.0
58	9310.5
59	8592.0
60	7770.5
61	6949.0
62	6589.0
63	6229.0
64	5170.0
65	4111.0
66	3485.0
67	2859.0
68	2522.5
69	2186.0
70	1683.0
71	1180.0
72	910.5
73	641.0
74	497.5
75	272.5
76	191.0
77	121.5
78	52.0
79	53.0
80	54.0
81	31.0
82	8.0
83	7.0
84	6.0
85	11.0
86	16.0
87	11.0
88	6.0
89	5.5
90	5.0
91	4.0
92	3.0
93	1.5
94	0.0
95	0.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	286976.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.283103813559322
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.66026176552492	22.41755408117752
2	11.495405179615705	7.192239072256913
3	4.716235032024506	4.426154103479036
4	2.409356725146199	3.014886262265834
5	1.392369813422445	2.1778824710080285
6	1.0036201615148983	1.8837812221231043
7	0.7641325536062379	1.6733106601248886
8	0.5368978000556948	1.3436663693131132
9	0.4232804232804233	1.1917372881355932
>10	5.127262600946811	38.641558876003565
>50	0.4154831523252576	7.721900089206065
>100	0.04232804232804233	2.298101583407672
>500	0.008911166805903648	1.8140889830508475
>1k	0.004455583402951824	4.203138938447815
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGC	3950	1.376421721677074	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCG	3277	1.1419073371989295	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCTTC	3237	1.1279688893844781	No Hit
GCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCTTCTGC	1598	0.5568409901873328	No Hit
CCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCTTCTGC	975	0.3397496654772525	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCT	813	0.28329895182872433	No Hit
CTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCTTCTGCT	721	0.2512405218554862	Illumina Single End Adapter 1 (95% over 21bp)
GGGTAAGGGGTATTAATAAATATTAGCCCACCAACAGCTACCATTACATTT	572	0.19931980374665478	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTC	572	0.19931980374665478	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGC	527	0.18363904995539695	No Hit
GAATATGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCTTC	516	0.17980597680642285	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCG	510	0.17771520963425513	No Hit
GAACTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCTTCT	440	0.1533229259589652	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGCTAGGATCGTA	422	0.14705062444246209	No Hit
TCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCTTCTGC	364	0.12683987511150757	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	344	0.11987065120428189	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCTTT	305	0.10628066458519178	No Hit
GCTGACTCTTATACACATCTGACGCAGCTAGGATCGTATGCCGTCTTCTGC	287	0.10000836306868867	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3205842997323818	0.0
2	0.0	0.0	0.0	1.8161797502230153	0.0
3	0.0	0.0	0.0	2.331553858162355	0.0
4	0.0	0.0	0.0	3.111758474576271	0.0
5	0.0	0.0	0.0	6.0579978813559325	0.0
6	0.0	0.0	0.0	7.049369982158787	0.0
7	0.0	0.0	0.0	8.449487065120428	0.0
8	0.0	0.0	0.0	10.390764384478144	0.0
9	0.0	0.0	0.0	11.006843777876895	0.0
10	0.0	0.0	0.0	13.688949598572703	0.0
11	0.0	0.0	0.0	15.445194023193578	0.0
12	0.0	0.0	0.0	18.50572870205174	0.0
13	0.0	0.0	0.0	19.169198818019627	0.0
14	0.0	0.0	0.0	19.467133140053523	0.0
15	0.0	0.0	0.0	20.1009840544157	0.0
16	0.0	0.0	0.0	21.133474576271187	0.0
17	0.0	0.0	0.0	22.193145071364853	0.0
18	0.0	0.0	0.0	23.346551628010705	0.0
19	0.0	0.0	0.0	24.06682091882248	0.0
20	0.0	0.0	0.0	24.735517952720784	0.0
21	0.0	0.0	0.0	25.5265248661909	0.0
22	0.0	0.0	0.0	26.28233719892953	0.0
23	0.0	0.0	0.0	27.095297167707404	0.0
24	0.0	0.0	0.0	27.736465767172167	0.0
25	0.0	0.0	0.0	28.267520628902766	0.0
26	0.0	0.0	0.0	28.766168599464763	0.0
27	0.0	0.0	0.0	29.228925066904548	0.0
28	0.0	0.0	0.0	29.740466101694917	0.0
29	0.0	0.0	0.0	30.26002174397859	0.0
30	0.0	0.0	0.0	30.838467328278323	0.0
31	0.0	0.0	0.0	31.40436830954505	0.0
32	0.0	0.0	0.0	31.9169547279215	0.0
33	0.0	0.0	0.0	32.42501115075825	0.0
34	0.0	0.0	0.0	32.91111451828724	0.0
35	0.0	0.0	0.0	33.44460860838537	0.0
36	0.0	0.0	0.0	33.9132889161463	0.0
37	0.0	0.0	0.0	34.429011485281	0.0
38	0.0	0.0	0.0	34.894904103479035	0.0
39	0.0	0.0	0.0	35.413065900981266	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTAGCG	30	2.1597898E-6	45.000004	1
GCAACGG	45	3.8198777E-10	45.000004	2
CGCATGG	30	2.1597898E-6	45.000004	2
GTCTCGC	30	2.1597898E-6	45.000004	31
TCGATCC	30	2.1597898E-6	45.000004	28
GTTCGCG	30	2.1597898E-6	45.000004	1
CTCGCGC	30	2.1597898E-6	45.000004	33
TACAAAC	30	2.1597898E-6	45.000004	30
TAATACG	55	1.8189894E-12	45.0	4
TCCGCGG	20	7.0244307E-4	45.0	14
GGTCGTT	40	6.7848305E-9	45.0	8
CTCCGCG	20	7.0244307E-4	45.0	13
GGTCGCT	20	7.0244307E-4	45.0	10
TCCGAAA	20	7.0244307E-4	45.0	25
ATAATTC	20	7.0244307E-4	45.0	40
GACGTTT	20	7.0244307E-4	45.0	28
TAGAACG	20	7.0244307E-4	45.0	1
GCTATAC	20	7.0244307E-4	45.0	30
ACTCTAA	35	1.2076634E-7	45.0	27
GATCCGC	20	7.0244307E-4	45.0	17
>>END_MODULE