##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936757.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	594038
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.0279965254748	33.0	31.0	34.0	30.0	34.0
2	32.11173359280046	33.0	31.0	34.0	30.0	34.0
3	32.06204148556153	33.0	31.0	34.0	30.0	34.0
4	35.74516445075904	37.0	35.0	37.0	35.0	37.0
5	30.96999013531121	37.0	35.0	37.0	0.0	37.0
6	33.29637666277242	37.0	35.0	37.0	17.0	37.0
7	35.23917325154283	37.0	35.0	37.0	32.0	37.0
8	35.63178954881674	37.0	35.0	37.0	33.0	37.0
9	37.62398870105953	39.0	37.0	39.0	35.0	39.0
10	37.36046010524579	39.0	37.0	39.0	34.0	39.0
11	37.1632740666422	39.0	37.0	39.0	34.0	39.0
12	37.054796157821556	39.0	37.0	39.0	33.0	39.0
13	36.91406442012127	39.0	37.0	39.0	33.0	39.0
14	38.10918325090314	40.0	37.0	41.0	33.0	41.0
15	38.22072998697053	40.0	37.0	41.0	33.0	41.0
16	38.1903464088156	40.0	37.0	41.0	34.0	41.0
17	38.16455849625782	40.0	37.0	41.0	34.0	41.0
18	38.09125678828627	40.0	37.0	41.0	33.0	41.0
19	38.06847036721557	40.0	37.0	41.0	34.0	41.0
20	38.07773913453348	40.0	37.0	41.0	34.0	41.0
21	37.99023968163653	40.0	36.0	41.0	34.0	41.0
22	38.00145781919675	40.0	36.0	41.0	34.0	41.0
23	37.98050966436491	40.0	36.0	41.0	34.0	41.0
24	37.94529137866601	40.0	36.0	41.0	34.0	41.0
25	37.80167430366407	40.0	36.0	41.0	33.0	41.0
26	37.71799447173413	40.0	36.0	41.0	33.0	41.0
27	37.664321137704995	40.0	36.0	41.0	33.0	41.0
28	37.63414966719301	40.0	36.0	41.0	33.0	41.0
29	37.57661462734707	40.0	36.0	41.0	33.0	41.0
30	37.48349263851807	40.0	36.0	41.0	33.0	41.0
31	37.394156602776256	40.0	35.0	41.0	33.0	41.0
32	37.30546025675125	39.0	35.0	41.0	32.0	41.0
33	37.22024348610695	39.0	35.0	41.0	32.0	41.0
34	37.09497708900778	39.0	35.0	41.0	31.0	41.0
35	36.99256444873897	39.0	35.0	41.0	31.0	41.0
36	36.910537709708805	39.0	35.0	41.0	31.0	41.0
37	36.86156777849229	39.0	35.0	41.0	31.0	41.0
38	36.766893700403	39.0	35.0	41.0	31.0	41.0
39	36.629915931304055	39.0	35.0	41.0	30.0	41.0
40	36.50687329766782	39.0	35.0	41.0	30.0	41.0
41	36.424659701904595	39.0	35.0	40.0	30.0	41.0
42	36.44601355468842	39.0	35.0	40.0	30.0	41.0
43	36.371688343169964	39.0	35.0	40.0	30.0	41.0
44	36.20961621983779	39.0	35.0	40.0	30.0	41.0
45	36.127942993545865	38.0	35.0	40.0	30.0	41.0
46	35.9346944134887	38.0	35.0	40.0	29.0	41.0
47	35.896616041398026	38.0	35.0	40.0	29.0	41.0
48	35.78042145451975	38.0	35.0	40.0	28.0	41.0
49	35.61760863783125	38.0	35.0	40.0	28.0	41.0
50	35.42893383924934	38.0	34.0	40.0	28.0	41.0
51	33.600808365794784	36.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	5.0
8	16.0
9	27.0
10	37.0
11	23.0
12	21.0
13	17.0
14	15.0
15	33.0
16	38.0
17	87.0
18	152.0
19	264.0
20	490.0
21	793.0
22	1225.0
23	1871.0
24	2597.0
25	3516.0
26	4588.0
27	5600.0
28	6958.0
29	8379.0
30	10412.0
31	13331.0
32	17619.0
33	25949.0
34	41435.0
35	51703.0
36	49675.0
37	76278.0
38	115929.0
39	154908.0
40	47.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.23942576064157	21.53060915294981	24.196600217494506	10.033364868914111
2	31.587709877145908	25.89059959127194	24.596911308704154	17.924779222877998
3	33.224810534006245	25.386423090778703	24.841676795087185	16.54708958012787
4	30.40478891922739	25.564694514492338	26.015170746652572	18.0153458196277
5	23.25373124278245	39.752507415350536	20.00612755412953	16.987633787737487
6	26.846430699719548	35.718253714408846	23.255414636774077	14.179900949097531
7	79.52841400718472	6.598062750194432	8.944040616930229	4.929482625690612
8	78.69378726613448	7.216036684521867	8.61813554015063	5.472040509193015
9	71.95835956622304	8.867446190311057	11.093229726044463	8.080964517421444
10	41.91078685201957	26.020220928627463	18.13150000505018	13.937492214302788
11	33.442641716523184	27.025038802231506	21.660903847901984	17.87141563334332
12	32.563405034694746	22.189152882475533	26.44376285692161	18.803679225908105
13	25.86534868139749	24.307872560341256	28.18658065645632	21.640198101804938
14	19.205673711109387	30.42650470171942	27.10887182301469	23.258949764156505
15	19.408017668903334	27.631733996815015	34.27255495439685	18.687693379884788
16	21.98411549429498	25.215221921829915	32.57081196825792	20.22985061561718
17	21.406711355165832	25.32329581609257	27.938111703291707	25.331881125449883
18	22.270124133472944	27.80041007477636	27.36626276433494	22.563203027415753
19	25.217241994619872	29.739174934936823	23.79174396250745	21.251839107935854
20	28.92845238856773	26.03351974116134	26.001703594719526	19.036324275551394
21	25.557792599126657	28.126315151555957	25.780505624219323	20.535386625098056
22	21.983947154895812	27.725835720947146	25.154956416929558	25.135260707227484
23	23.593271810894255	27.946865352048185	26.844747305727918	21.615115531329646
24	24.473855207915992	24.25316225561328	28.0492157067393	23.22376682973143
25	22.714035129065817	26.868988179207392	25.837404341136427	24.579572350590368
26	22.327864547385857	28.78368050528754	26.28636551870419	22.60208942862241
27	23.148014100108075	28.978112511320823	25.313868809739443	22.56000457883166
28	20.362670401556805	29.31108784286527	26.706540659015083	23.619701096562846
29	23.80857790242375	27.551267764015098	26.088566724687645	22.551587608873504
30	23.924227069648744	25.28945959686081	26.093448567263373	24.69286476622708
31	23.284200674030956	26.992717637592207	24.793868405724886	24.92921328265195
32	25.96753069668944	27.573993582902105	25.3204340463068	21.138041674101657
33	24.86002578959595	26.757379157562312	25.341981489399668	23.04061356344207
34	23.43688450907181	25.145192731778103	28.839232507011335	22.578690252138752
35	22.658651466741183	24.339520367383905	28.163854837569314	24.837973328305594
36	22.571788336773068	27.094226295287505	27.38478009824287	22.949205269696552
37	22.249586726775057	27.16577053993179	28.70843279386167	21.876209939431483
38	22.134779256545876	25.857268390237664	27.615236735697042	24.392715617519418
39	24.87399795972648	22.989438386096513	29.557031705042437	22.57953194913457
40	24.414936418208935	22.667910133695152	31.710934317333233	21.20621913076268
41	19.654129870479668	25.769563563273728	30.76974873661281	23.806557829633796
42	21.82520310148509	25.252593268444105	29.49188435756635	23.430319272504452
43	23.17898854955407	24.934095125227678	27.47837680417751	24.408539521040744
44	22.39435861005525	25.840602789720524	26.93649227827176	24.82854632195247
45	22.64215420562321	23.834670509294018	26.466993694006106	27.056181591076665
46	22.318774219831056	26.404876455714955	26.845252323925408	24.431097000528585
47	20.968860577942824	25.23138250414957	30.58289200354186	23.216864914365747
48	21.409236446153276	22.667573454896825	29.45316629575886	26.47002380319104
49	20.79883778478818	23.961430076863767	31.26416155195459	23.975570586393463
50	19.75530184937664	23.86547661934085	30.311697231490243	26.06752429979227
51	19.33933519404483	23.278308795060248	28.734357061332776	28.647998949562147
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	121.0
1	326.0
2	531.0
3	849.0
4	1167.0
5	854.0
6	541.0
7	482.5
8	424.0
9	444.0
10	464.0
11	483.0
12	502.0
13	532.5
14	563.0
15	627.5
16	692.0
17	700.5
18	709.0
19	767.5
20	826.0
21	1371.5
22	1917.0
23	2012.0
24	2107.0
25	3090.0
26	4659.0
27	5245.0
28	6624.5
29	8004.0
30	9530.5
31	11057.0
32	12031.5
33	13006.0
34	13859.5
35	14713.0
36	16355.0
37	17997.0
38	19384.0
39	20771.0
40	23869.0
41	26967.0
42	30224.0
43	33481.0
44	37217.0
45	40953.0
46	48509.0
47	56065.0
48	56263.0
49	56461.0
50	55509.5
51	54558.0
52	47966.0
53	41374.0
54	37240.0
55	33106.0
56	31116.0
57	29126.0
58	29379.5
59	29633.0
60	30464.5
61	31296.0
62	27668.0
63	24040.0
64	18818.0
65	13596.0
66	10598.5
67	7601.0
68	5722.5
69	3844.0
70	3328.0
71	2812.0
72	2330.0
73	1848.0
74	1352.5
75	691.0
76	525.0
77	375.5
78	226.0
79	147.5
80	69.0
81	65.5
82	62.0
83	62.5
84	63.0
85	35.5
86	8.0
87	9.0
88	10.0
89	9.5
90	9.0
91	5.0
92	1.0
93	2.5
94	4.0
95	6.5
96	9.0
97	6.0
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	594038.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.944119311541122
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.68660132597067	22.492228637812598
2	10.356182026498486	6.409258644800136
3	4.007360358017763	3.7201271112852554
4	2.1921988654591122	2.7134265298956746
5	1.392403205669355	2.154334546300243
6	1.0258797108483901	1.9046966503069136
7	0.7723963604468546	1.6730787593427314
8	0.5984468222246905	1.481472789482677
9	0.49726024359507853	1.3848552276022903
>10	5.972223030534368	43.34164606918709
>50	0.43673597368209677	8.12895178877057
>100	0.057900602571488664	2.993646595902262
>500	0.002205737240818616	0.5233421175120974
>1k	0.002205737240818616	1.0789345317994756
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGC	1696	0.285503620980476	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTC	1594	0.2683330022658483	No Hit
GCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGC	1558	0.2622727838959797	TruSeq Adapter, Index 20 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCG	1475	0.24830061376544935	No Hit
CCTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGC	950	0.15992242920486568	TruSeq Adapter, Index 20 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCGCCTGTTTTCGTATGCCGTCTTCTGCT	835	0.14056339830111877	TruSeq Adapter, Index 22 (95% over 23bp)
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	648	0.10908393065763469	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	634	0.10672717906935246	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.683393991630165E-4	0.0	0.0	0.18736175126843738	0.0
2	1.683393991630165E-4	0.0	0.0	0.9810820183220602	0.0
3	1.683393991630165E-4	0.0	0.0	1.2972234099502051	0.0
4	1.683393991630165E-4	0.0	0.0	1.7591467212535226	0.0
5	1.683393991630165E-4	0.0	0.0	3.2592191071951624	0.0
6	1.683393991630165E-4	0.0	0.0	4.123641921897252	0.0
7	1.683393991630165E-4	0.0	0.0	5.0259411014110205	0.0
8	1.683393991630165E-4	0.0	0.0	6.313232486810608	0.0
9	1.683393991630165E-4	0.0	0.0	6.769937276739872	0.0
10	1.683393991630165E-4	0.0	0.0	8.048643352782145	0.0
11	1.683393991630165E-4	0.0	0.0	9.354957090287153	0.0
12	1.683393991630165E-4	0.0	0.0	10.735676842222215	0.0
13	1.683393991630165E-4	0.0	0.0	11.23261474855144	0.0
14	1.683393991630165E-4	0.0	0.0	11.463071386005609	0.0
15	1.683393991630165E-4	0.0	0.0	11.855638864853763	0.0
16	1.683393991630165E-4	0.0	0.0	12.64010046495342	0.0
17	1.683393991630165E-4	0.0	0.0	13.492402842915773	0.0
18	1.683393991630165E-4	0.0	0.0	14.430726653850428	0.0
19	1.683393991630165E-4	0.0	0.0	14.998535447227281	0.0
20	1.683393991630165E-4	0.0	0.0	15.54698520970039	0.0
21	1.683393991630165E-4	0.0	0.0	16.224214612533206	0.0
22	1.683393991630165E-4	0.0	0.0	16.953460889707394	0.0
23	1.683393991630165E-4	0.0	0.0	17.63742386850673	0.0
24	1.683393991630165E-4	0.0	0.0	18.20725273467354	0.0
25	1.683393991630165E-4	0.0	0.0	18.703012265208624	0.0
26	1.683393991630165E-4	0.0	0.0	19.161232109730353	0.0
27	1.683393991630165E-4	0.0	0.0	19.60918325090314	0.0
28	1.683393991630165E-4	0.0	0.0	20.04888576151694	0.0
29	1.683393991630165E-4	0.0	0.0	20.529326406728188	0.0
30	1.683393991630165E-4	0.0	0.0	21.04343493177204	0.0
31	1.683393991630165E-4	0.0	0.0	21.5912113366485	0.0
32	1.683393991630165E-4	0.0	0.0	22.08528747319195	0.0
33	1.683393991630165E-4	0.0	0.0	22.572966712567208	0.0
34	1.683393991630165E-4	0.0	0.0	23.04398035142533	0.0
35	1.683393991630165E-4	0.0	0.0	23.521390887451645	0.0
36	1.683393991630165E-4	0.0	0.0	24.06849393473145	0.0
37	1.683393991630165E-4	0.0	0.0	24.584454193166092	0.0
38	1.683393991630165E-4	0.0	0.0	25.07869866910871	0.0
39	1.683393991630165E-4	0.0	0.0	25.57361650264798	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGCGATG	70	0.0	45.000004	1
CGTAAGG	140	0.0	45.000004	2
ACGTAAG	35	1.2106102E-7	45.000004	1
CGAACTT	20	7.030531E-4	45.0	18
ACCGGGT	20	7.030531E-4	45.0	4
GACCCGT	20	7.030531E-4	45.0	18
TAGTTCG	20	7.030531E-4	45.0	1
ATATCGC	20	7.030531E-4	45.0	27
ACGGCAC	25	3.8885213E-5	45.0	38
GTTCGCA	20	7.030531E-4	45.0	43
TACGAGA	20	7.030531E-4	45.0	35
ATCCCGA	25	3.8885213E-5	45.0	10
AGTTCGC	20	7.030531E-4	45.0	42
TCCGTCA	20	7.030531E-4	45.0	28
ACATGCG	20	7.030531E-4	45.0	1
CGTGTAA	20	7.030531E-4	45.0	19
CCGGTTA	20	7.030531E-4	45.0	14
GGACCGT	25	3.8885213E-5	45.0	8
TACGTAG	50	2.1827873E-11	45.0	1
ACGAACT	20	7.030531E-4	45.0	17
>>END_MODULE