##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936756.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	761961
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.09772022452593	33.0	31.0	34.0	30.0	34.0
2	32.18188857434961	33.0	31.0	34.0	30.0	34.0
3	32.116848500120085	33.0	31.0	34.0	30.0	34.0
4	35.79486876624919	37.0	35.0	37.0	35.0	37.0
5	31.04249036368003	37.0	35.0	37.0	0.0	37.0
6	33.34276819942228	37.0	35.0	37.0	17.0	37.0
7	35.2461884532148	37.0	35.0	37.0	32.0	37.0
8	35.64766569417595	37.0	35.0	37.0	34.0	37.0
9	37.67079679931125	39.0	37.0	39.0	35.0	39.0
10	37.42435242748645	39.0	37.0	39.0	35.0	39.0
11	37.220208120888074	39.0	37.0	39.0	34.0	39.0
12	37.12587389643302	39.0	37.0	39.0	34.0	39.0
13	36.997060216992736	39.0	37.0	39.0	33.0	39.0
14	38.20502361669429	40.0	37.0	41.0	33.0	41.0
15	38.23465111731441	40.0	37.0	41.0	33.0	41.0
16	38.25654331389664	40.0	37.0	41.0	34.0	41.0
17	38.21169849900454	40.0	37.0	41.0	34.0	41.0
18	38.134673559407894	40.0	37.0	41.0	33.0	41.0
19	38.06764913164847	40.0	37.0	41.0	34.0	41.0
20	38.08753203904137	40.0	37.0	41.0	34.0	41.0
21	38.04641444903348	40.0	37.0	41.0	34.0	41.0
22	38.06442061995299	40.0	37.0	41.0	34.0	41.0
23	38.06815440685284	40.0	37.0	41.0	34.0	41.0
24	38.01697068485132	40.0	36.0	41.0	34.0	41.0
25	37.866767459226914	40.0	36.0	41.0	33.0	41.0
26	37.77439658985171	40.0	36.0	41.0	33.0	41.0
27	37.74972209863759	40.0	36.0	41.0	33.0	41.0
28	37.715848973897614	40.0	36.0	41.0	33.0	41.0
29	37.65339564623386	40.0	36.0	41.0	33.0	41.0
30	37.57745081441176	40.0	36.0	41.0	33.0	41.0
31	37.49563297859077	40.0	36.0	41.0	33.0	41.0
32	37.41435716526174	40.0	36.0	41.0	33.0	41.0
33	37.326321163419124	40.0	35.0	41.0	32.0	41.0
34	37.209332236164315	40.0	35.0	41.0	32.0	41.0
35	37.13051061668511	40.0	35.0	41.0	32.0	41.0
36	36.97086465055298	39.0	35.0	41.0	31.0	41.0
37	36.93445990017862	39.0	35.0	41.0	31.0	41.0
38	36.86479885453455	39.0	35.0	41.0	31.0	41.0
39	36.73341942697854	39.0	35.0	41.0	31.0	41.0
40	36.62020365871744	39.0	35.0	41.0	30.0	41.0
41	36.557347423293315	39.0	35.0	41.0	31.0	41.0
42	36.49531012742122	39.0	35.0	41.0	30.0	41.0
43	36.42791953918901	39.0	35.0	41.0	30.0	41.0
44	36.277297656966695	39.0	35.0	40.0	30.0	41.0
45	36.20952384702104	38.0	35.0	40.0	30.0	41.0
46	36.03595065889199	38.0	35.0	40.0	29.0	41.0
47	35.88556894644214	38.0	35.0	40.0	29.0	41.0
48	35.78472257766474	38.0	35.0	40.0	29.0	41.0
49	35.663372534814776	38.0	35.0	40.0	28.0	41.0
50	35.49218004596036	38.0	34.0	40.0	28.0	41.0
51	33.69729028126111	36.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	29.0
9	55.0
10	64.0
11	36.0
12	31.0
13	23.0
14	23.0
15	43.0
16	63.0
17	121.0
18	244.0
19	440.0
20	650.0
21	1086.0
22	1666.0
23	2287.0
24	3149.0
25	4359.0
26	5515.0
27	6577.0
28	8000.0
29	10039.0
30	12655.0
31	16808.0
32	22471.0
33	32674.0
34	51776.0
35	64042.0
36	64963.0
37	99098.0
38	150190.0
39	202695.0
40	86.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.88607553404964	20.770616868842367	22.75339551499355	10.589912082114438
2	31.932867955184058	26.59204342479471	24.36358291303623	17.111505706985003
3	32.33039486272919	26.95886009913893	24.50125400118904	16.209491036942836
4	28.596607962874742	24.8091437750751	27.481459024805734	19.112789237244428
5	23.255652192172565	39.0156451576918	20.66654330077261	17.062159349363025
6	26.351742412013213	35.20495143452224	24.22512438300648	14.218181770458068
7	78.3856391600095	6.08784439098589	10.814857978295477	4.71165847070913
8	78.62370908747297	7.251683485112754	9.011747320400913	5.1128601070133515
9	71.71495653977041	8.018258152320131	10.626659369705274	9.640125938204186
10	41.97380180875399	23.320353666395	18.719330779396845	15.986513745454165
11	30.52951529015265	25.464820377945852	24.41292927065821	19.592735061243292
12	27.614274221384033	22.7090362892589	28.757508586397467	20.9191809029596
13	24.364501595226002	24.017239727492615	31.740469656583475	19.877789020697907
14	21.53758525698822	27.643015849892578	28.704487500016405	22.114911393102794
15	19.798782352377614	25.60341014828843	34.416328394760356	20.181479104573594
16	23.719192977068378	25.507735960239437	28.73651013634556	22.036560926346624
17	23.478498243348415	25.043670214092323	27.924395080588116	23.55343646197115
18	24.276176864695177	25.07661153261125	28.9555502184495	21.691661384244078
19	23.72549251208395	27.970460430389483	26.068263336312487	22.235783721214077
20	25.769166663385658	27.142859017718756	27.546816700592284	19.5411576183033
21	25.04905106691812	27.934631824988422	27.921770274331625	19.094546833761832
22	23.796099800383484	23.82431646764073	27.373841968289714	25.005741763686068
23	22.443668376727942	27.518468793022215	28.448175169070332	21.58968766117951
24	24.256622058084336	24.520808807799874	27.948149577209332	23.274419556906455
25	22.160976742904165	28.915259442412406	25.620865109894076	23.302898704789353
26	21.33376905117191	27.65732104399044	26.90085188087054	24.108058023967104
27	22.36321806496658	28.503821061707885	26.925656299994355	22.20730457333118
28	20.16979871673222	26.83615040664811	29.39271170046761	23.601339176152063
29	22.33920108771971	27.94893701908628	26.98261459576015	22.729247297433858
30	22.606012643691738	28.598576567567108	27.377910417987273	21.417500370753885
31	24.104514535520845	26.707534900080187	26.75596257551239	22.43198798888657
32	26.362766598290467	27.887647792997278	25.02135936091217	20.728226247800087
33	24.99904850773202	26.4167063668613	25.180685100681004	23.403560024725675
34	22.87216799809964	25.762473407431614	27.774912364281114	23.590446230187634
35	23.89505499625309	27.739477479818518	25.449727741971046	22.915739781957345
36	23.627718479029767	30.13120094073056	25.733600538610247	20.507480041629428
37	23.41944010257743	28.39305423768408	27.399696309916123	20.787809349822368
38	24.475399659562626	27.67018259464723	25.250636187416415	22.603781558373722
39	24.932247188504398	25.772316430893444	25.69711573164506	23.5983206489571
40	26.38586489334756	24.413979193160802	27.31268398251354	21.887471930978094
41	21.351748974028855	27.32226452534972	26.162887601858888	25.163098898762538
42	23.07454056047488	25.711945886994215	28.13070485234809	23.082808700182817
43	23.738091582115096	25.878489843968396	26.496237996432885	23.887180577483626
44	23.048423738222823	25.285808591253357	27.065558473465178	24.600209197058643
45	23.040811800079005	24.21738120454984	26.210659075726973	26.531147919644184
46	24.817805635721513	25.815494493812675	25.957758992914336	23.408940877551476
47	21.044384161394085	25.783865578421995	30.428197768652197	22.74355249153172
48	22.284736357897582	25.819694183823056	27.648790423656855	24.246779034622506
49	22.531336905694648	24.35190252519486	29.617132635397347	23.499627933713143
50	22.078820307076082	23.706068945785937	29.909667292682958	24.305443454455016
51	21.55333409452715	23.75187181496166	26.81922040629376	27.875573684217436
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	195.0
1	543.0
2	891.0
3	1582.0
4	2273.0
5	1594.5
6	916.0
7	895.5
8	875.0
9	857.5
10	840.0
11	904.0
12	968.0
13	963.0
14	958.0
15	1119.5
16	1281.0
17	1274.5
18	1268.0
19	1415.0
20	1562.0
21	1849.5
22	2137.0
23	2755.5
24	3374.0
25	3922.0
26	5920.5
27	7371.0
28	7921.5
29	8472.0
30	9889.5
31	11307.0
32	13528.5
33	15750.0
34	18120.5
35	20491.0
36	21787.5
37	23084.0
38	25987.5
39	28891.0
40	31917.0
41	34943.0
42	37648.5
43	40354.0
44	45965.5
45	51577.0
46	66737.5
47	81898.0
48	76239.0
49	70580.0
50	69437.5
51	68295.0
52	61437.5
53	54580.0
54	48810.5
55	43041.0
56	40102.0
57	37163.0
58	35494.0
59	33825.0
60	31910.0
61	29995.0
62	26675.0
63	23355.0
64	20699.5
65	18044.0
66	15322.5
67	12601.0
68	10552.0
69	8503.0
70	7414.0
71	6325.0
72	5427.5
73	4530.0
74	3588.0
75	1982.0
76	1318.0
77	939.5
78	561.0
79	388.5
80	216.0
81	159.5
82	103.0
83	77.5
84	52.0
85	37.5
86	23.0
87	18.5
88	14.0
89	12.0
90	10.0
91	9.0
92	8.0
93	7.5
94	7.0
95	12.5
96	18.0
97	10.0
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	761961.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.40220258037093
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.27428542378127	21.250231813815667
2	10.250353043021557	6.027659133824828
3	3.890608675456514	3.4317739331016304
4	2.1766500653497363	2.559932246719623
5	1.3844572802309947	2.035304670861054
6	1.049657630058286	1.8517347767403463
7	0.8029991685829189	1.6526960958581087
8	0.6095915455221419	1.433866729017874
9	0.5349778008721032	1.4156573109518626
>10	6.6144927238581825	45.87900915381462
>50	0.36309231015800575	6.331509181356252
>100	0.041599617092228494	2.1699750720659496
>500	0.004069527759022353	0.9403402576107377
>1k	0.0018086790040099346	0.7365311787308761
>5k	0.0013565092530074508	2.2837784455305035
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGC	6035	0.7920352878953122	No Hit
GAATCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTC	5835	0.7657872253304303	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCG	5308	0.6966235804719665	No Hit
GCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGC	2198	0.2884662075880524	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCT	1182	0.1551260497584522	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1101	0.14449558441967503	No Hit
CCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGC	1059	0.1389834912810498	No Hit
GAACTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCT	918	0.12047860717280805	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTC	903	0.11851000248044191	No Hit
CTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTCTGCT	898	0.11785380091631986	Illumina Single End Adapter 1 (95% over 21bp)
GAATAATACCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCG	853	0.11194798683922143	No Hit
GAATATGTCTCTTATACACATCTGACGCAATGGCGATCGTATGCCGTCTTC	836	0.10971690152120647	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCAATGGCGATCGTATGC	808	0.106042172762123	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.14764535192746087	0.0
2	0.0	0.0	0.0	0.8739292430977439	0.0
3	0.0	0.0	0.0	1.193105683886708	0.0
4	0.0	0.0	0.0	1.6549403447158058	0.0
5	0.0	0.0	0.0	3.5063474377297528	0.0
6	0.0	0.0	0.0	4.279746601203999	0.0
7	0.0	0.0	0.0	5.112203905449229	0.0
8	0.0	0.0	0.0	6.228665246646482	0.0
9	0.0	0.0	0.0	6.599156649749791	0.0
10	0.0	0.0	0.0	8.247928699762848	0.0
11	0.0	0.0	0.0	9.397200119166204	0.0
12	0.0	0.0	0.0	11.206216591137867	0.0
13	0.0	0.0	0.0	11.712147997075967	0.0
14	0.0	0.0	0.0	11.926463427918227	0.0
15	0.0	0.0	0.0	12.406146771291445	0.0
16	0.0	0.0	0.0	13.075472366695934	0.0
17	0.0	0.0	0.0	13.801756257866217	0.0
18	0.0	0.0	0.0	14.5613751884939	0.0
19	0.0	0.0	0.0	15.056282408154749	0.0
20	0.0	0.0	0.0	15.519560712424914	0.0
21	0.0	0.0	0.0	16.09714932916514	0.0
22	0.0	0.0	0.0	16.67828143435163	0.0
23	0.0	0.0	0.0	17.23014694977827	0.0
24	0.0	0.0	0.0	17.6905379671663	0.0
25	0.0	0.0	0.0	18.114312937276317	0.0
26	0.0	0.0	0.0	18.50882131762649	0.0
27	0.0	0.0	0.0	18.91435388425392	0.0
28	0.0	0.0	0.0	19.303743892403944	0.0
29	0.0	0.0	0.0	19.736180723160373	0.0
30	0.0	0.0	0.0	20.242768330662592	0.0
31	0.0	0.0	0.0	20.686623068634745	0.0
32	0.0	0.0	0.0	21.115778891570567	0.0
33	0.0	0.0	0.0	21.537847737613866	0.0
34	0.0	0.0	0.0	21.97133449087289	0.0
35	0.0	0.0	0.0	22.43736884171237	0.0
36	0.0	0.0	0.0	22.856287920247887	0.0
37	0.0	0.0	0.0	23.289643433194087	0.0
38	0.0	0.0	0.0	23.72877351990456	0.0
39	0.0	0.0	0.0	24.264627717166626	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGACATA	25	3.889564E-5	45.0	20
CGTTATA	20	7.0317875E-4	45.0	36
TGGCACG	20	7.0317875E-4	45.0	1
CGCTAAT	20	7.0317875E-4	45.0	41
TCGCTCG	25	3.889564E-5	45.0	41
CGGTGTA	20	7.0317875E-4	45.0	21
GCGTGTA	20	7.0317875E-4	45.0	35
CGGTCTA	55	1.8189894E-12	45.0	31
CGTTTAA	20	7.0317875E-4	45.0	24
CAAAGCG	25	3.889564E-5	45.0	45
TATCCGG	20	7.0317875E-4	45.0	2
TAATACG	145	0.0	41.89655	4
ACGGGTA	100	0.0	40.5	5
TGTACGG	125	0.0	39.600002	2
ACAACGA	215	0.0	38.720932	13
AACGTAC	35	6.2455183E-6	38.571426	24
CGAGTAT	35	6.2455183E-6	38.571426	12
TTAACGG	70	0.0	38.571426	2
TATAGCG	35	6.2455183E-6	38.571426	1
TACGGCT	900	0.0	38.5	7
>>END_MODULE