##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936755.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	453643
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.08159720308701	33.0	31.0	34.0	30.0	34.0
2	32.15636083880937	33.0	31.0	34.0	30.0	34.0
3	32.122003425601186	33.0	31.0	34.0	30.0	34.0
4	35.794468337437145	37.0	35.0	37.0	35.0	37.0
5	30.925741166511994	37.0	35.0	37.0	0.0	37.0
6	33.26322901488616	37.0	35.0	37.0	17.0	37.0
7	35.18143562228448	37.0	35.0	37.0	32.0	37.0
8	35.549961092753556	37.0	35.0	37.0	33.0	37.0
9	37.61571103268429	39.0	37.0	39.0	35.0	39.0
10	37.40478305627994	39.0	37.0	39.0	35.0	39.0
11	37.20366235123214	39.0	37.0	39.0	34.0	39.0
12	37.06562429046629	39.0	37.0	39.0	33.0	39.0
13	36.844232138487754	39.0	35.0	39.0	33.0	39.0
14	38.09415774077854	40.0	37.0	41.0	33.0	41.0
15	38.09088644594979	40.0	37.0	41.0	33.0	41.0
16	38.19353764964961	40.0	37.0	41.0	34.0	41.0
17	38.11218954111493	40.0	37.0	41.0	33.0	41.0
18	38.03997196033004	40.0	37.0	41.0	33.0	41.0
19	37.97531979993078	40.0	37.0	41.0	34.0	41.0
20	37.99521429846818	40.0	36.0	41.0	33.0	41.0
21	38.00777263178314	40.0	36.0	41.0	34.0	41.0
22	38.03118972407818	40.0	36.0	41.0	34.0	41.0
23	38.08652178034269	40.0	36.0	41.0	34.0	41.0
24	38.000410014041876	40.0	36.0	41.0	34.0	41.0
25	37.89160859971387	40.0	36.0	41.0	34.0	41.0
26	37.80567979666831	40.0	36.0	41.0	33.0	41.0
27	37.81090416913741	40.0	36.0	41.0	33.0	41.0
28	37.734972213833345	40.0	36.0	41.0	33.0	41.0
29	37.693499073059655	40.0	36.0	41.0	33.0	41.0
30	37.66779163350917	40.0	36.0	41.0	33.0	41.0
31	37.552778286009044	40.0	36.0	41.0	33.0	41.0
32	37.47801024153354	40.0	36.0	41.0	33.0	41.0
33	37.404287071551856	40.0	36.0	41.0	33.0	41.0
34	37.225108730874275	39.0	35.0	41.0	32.0	41.0
35	37.16541862213238	39.0	35.0	41.0	32.0	41.0
36	37.05964602121051	39.0	35.0	41.0	32.0	41.0
37	37.050577216004655	39.0	35.0	41.0	32.0	41.0
38	36.92879643243696	39.0	35.0	41.0	31.0	41.0
39	36.79907107571372	39.0	35.0	41.0	31.0	41.0
40	36.555416924762426	39.0	35.0	41.0	30.0	41.0
41	36.45409275575728	39.0	35.0	41.0	30.0	41.0
42	36.40497704141803	39.0	35.0	41.0	30.0	41.0
43	36.401725585978404	39.0	35.0	40.0	30.0	41.0
44	36.31928190228881	39.0	35.0	40.0	30.0	41.0
45	36.26562076346378	39.0	35.0	40.0	30.0	41.0
46	35.93471518352537	38.0	35.0	40.0	29.0	41.0
47	35.75174972390184	38.0	35.0	40.0	28.0	41.0
48	35.61847091214898	38.0	35.0	40.0	28.0	41.0
49	35.51991984886794	38.0	35.0	40.0	28.0	41.0
50	35.351595858417305	38.0	34.0	40.0	27.0	41.0
51	33.41157694486634	36.0	32.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	15.0
9	32.0
10	31.0
11	32.0
12	15.0
13	8.0
14	16.0
15	19.0
16	28.0
17	65.0
18	160.0
19	224.0
20	357.0
21	614.0
22	823.0
23	1283.0
24	1787.0
25	2460.0
26	3253.0
27	4019.0
28	4863.0
29	6013.0
30	7815.0
31	10509.0
32	14004.0
33	20137.0
34	31810.0
35	40639.0
36	37939.0
37	57132.0
38	87529.0
39	119978.0
40	33.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.57948430814539	20.168282107295827	22.00122122461936	10.251012359939423
2	29.567523360880692	30.596305905745268	23.611518308449597	16.224652424924447
3	31.090968007882854	28.89915638508695	24.779176577176326	15.230699029853872
4	28.30221121013661	24.098465092594836	30.284827496511575	17.31449620075698
5	25.477963949625586	37.41179738252326	19.690814142398317	17.419424525452833
6	24.794827650817933	37.51143520345293	23.54604832434315	14.147688821385978
7	75.62025645717007	6.811523598953362	12.831896447206285	4.7363234966702885
8	74.26302180348866	10.126024208463482	9.654287622646002	5.956666365401868
9	68.60284408664964	7.905776127924382	11.245847505637693	12.245532279788291
10	41.3770299552732	24.09163152523019	19.143467440255886	15.387871079240725
11	33.51115304325207	25.186104491858135	22.809566112559875	18.493176352329915
12	30.48674839025401	21.080673569304498	28.14217347120974	20.29040456923175
13	25.1307746399702	24.546614849121447	31.2197035995265	19.102906911381858
14	20.495411590171127	30.26983773584074	26.198354212453406	23.036396461534732
15	17.932382953115113	25.99775594465251	37.991548420233535	18.07831268199884
16	20.028745070462897	26.06587118064205	30.005312547531872	23.900071201363186
17	19.76444031981095	25.677019153828013	29.623073650425557	24.935466875935482
18	21.26870689066072	26.726963713757296	29.308288676338	22.69604071924399
19	23.64326133104666	28.169728178325247	25.71228917893586	22.47472131169223
20	25.237466465921443	27.188339729699347	29.513075259620454	18.061118544758763
21	23.35625150173154	29.71631877930443	27.12948287530062	19.797946843663407
22	20.231327277176106	25.483254453391762	27.712099602550904	26.573318666881224
23	21.090813701523004	29.596180256280817	27.668012071166093	21.644993971030082
24	23.814761828133577	24.02638197878067	26.592055867719772	25.56680032536598
25	20.138743461268003	31.038283407878005	25.577381332898337	23.24559179795566
26	19.72145497671076	27.528034159019317	28.030191141492317	24.720319722777603
27	22.585381015468112	29.59287369142696	26.185348390694884	21.636396902410045
28	18.395963345626406	28.085961868694103	30.259477165965308	23.25859761971418
29	20.63979825545638	25.662470268471022	29.718743593530593	23.978987882542
30	21.66329029655478	26.43554513130369	30.203265563449673	21.697899008691856
31	24.147843127745826	28.72523107377387	23.972374752834277	23.154551045646027
32	24.514210513553607	29.08520576753086	24.875287395595215	21.52529632332032
33	25.17265779478577	25.57649958227064	24.353731899312898	24.897110723630696
34	25.48567926761793	25.560407633315187	27.577853069484153	21.37606002958273
35	21.096985955916878	25.934490337115307	27.296133743935208	25.672389963032604
36	22.646883121749923	27.94818833311657	26.899345961471905	22.505582583661603
37	21.41617968314291	27.003392535540062	29.679064815284267	21.90136296603276
38	21.970359952649993	29.586480999376164	23.42811417788878	25.015044870085067
39	25.940662591509184	22.955054966129754	26.238694303670506	24.865588138690555
40	25.89326849527051	24.472768234051888	28.986008821915032	20.647954448762572
41	20.931216837909986	26.272200827522962	27.012210041817024	25.784372292750025
42	20.66294420943341	26.684419245970954	29.279852218594797	23.372784326000843
43	22.992529367806842	24.395174178814617	26.744157850997368	25.86813860238117
44	22.619989727605187	24.856770632413593	27.48857581842991	25.034663821551305
45	21.348946197781075	23.74973271934098	26.53606470286106	28.365256380016884
46	25.17067385587345	27.375270862770947	24.98638797468494	22.467667306670663
47	20.34066435501044	24.284514475038744	32.77709564569496	22.597725524255857
48	22.10835392588445	24.885207090156797	27.394008063609494	25.61243092034926
49	20.412967906481526	23.245150922641812	31.85478448912471	24.48709668175195
50	20.524068485571252	23.553763642335493	29.42445932153698	26.497708550556275
51	20.59879685126851	23.44486743981501	25.790324109486974	30.16601159942951
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	156.0
1	343.5
2	531.0
3	853.0
4	1175.0
5	864.0
6	553.0
7	514.5
8	476.0
9	484.0
10	492.0
11	498.0
12	504.0
13	478.5
14	453.0
15	548.0
16	643.0
17	630.0
18	617.0
19	625.5
20	634.0
21	1179.5
22	1725.0
23	1645.5
24	1566.0
25	1996.0
26	3476.5
27	4527.0
28	5394.5
29	6262.0
30	7074.5
31	7887.0
32	8967.5
33	10048.0
34	11056.5
35	12065.0
36	12335.0
37	12605.0
38	14564.0
39	16523.0
40	18876.0
41	21229.0
42	23250.5
43	25272.0
44	30505.0
45	35738.0
46	48875.5
47	62013.0
48	51918.0
49	41823.0
50	40413.5
51	39004.0
52	33624.5
53	28245.0
54	25127.5
55	22010.0
56	20622.5
57	19235.0
58	19072.0
59	18909.0
60	17966.0
61	17023.0
62	15874.5
63	14726.0
64	12224.0
65	9722.0
66	7596.0
67	5470.0
68	4674.5
69	3879.0
70	3253.5
71	2628.0
72	2225.5
73	1823.0
74	1642.0
75	1158.5
76	856.0
77	599.5
78	343.0
79	253.5
80	164.0
81	136.0
82	108.0
83	76.0
84	44.0
85	33.0
86	22.0
87	16.0
88	10.0
89	8.5
90	7.0
91	7.0
92	7.0
93	4.0
94	1.0
95	0.5
96	0.0
97	1.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	453643.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.833905469977495
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.32250985411022	20.853404124849302
2	10.558535300832693	6.088876175312605
3	4.062261984664003	3.5139263418025513
4	2.2181338408803075	2.558298459508036
5	1.4869492222291114	2.143727665620538
6	1.0218533691962772	1.7678414070950081
7	0.8044366259861716	1.623653474119104
8	0.5844513088260752	1.348161103239654
9	0.4526755563367383	1.1747163779984686
>10	5.9734883252312425	41.745247647293084
>50	0.4564939710973912	7.956468657328891
>100	0.04672169838412897	2.4538352694251895
>500	0.006127435853656257	1.3301637861351738
>1k	0.0030637179268281287	1.25065872835522
>5k	0.002297788445121097	4.191020781917183
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGC	7258	1.5999365139548059	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCG	6046	1.3327660737628488	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTC	5673	1.2505428277301756	No Hit
GCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTCTGC	1958	0.4316169322573037	TruSeq Adapter, Index 21 (95% over 23bp)
GAATGACTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCT	1311	0.2889937682274388	No Hit
GAACTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTCT	1286	0.28348282680433734	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGC	1108	0.24424492387185517	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTC	986	0.21735152972712024	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCG	978	0.21558802847172778	No Hit
GAATATGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTC	894	0.197071265290107	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTT	807	0.17789318913771401	No Hit
CCTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTCTGC	653	0.14394578997140925	TruSeq Adapter, Index 21 (95% over 23bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTTACGGACTCGTA	644	0.14196185105909273	No Hit
CTGTCTCTTATACACATCTGACGCTTACGGACTCGTATGCCGTCTTCTGCT	541	0.11925677239591485	Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	520	0.11462758160050966	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	454	0.10007869624352188	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.16643043097766305	0.0
2	0.0	0.0	0.0	1.1498028185158815	0.0
3	0.0	0.0	0.0	1.4359308972033074	0.0
4	0.0	0.0	0.0	2.1829941165189366	0.0
5	0.0	0.0	0.0	4.9164210623772435	0.0
6	0.0	0.0	0.0	5.7219002607777485	0.0
7	0.0	0.0	0.0	6.823206794770337	0.0
8	0.0	0.0	0.0	8.208437030881111	0.0
9	0.0	0.0	0.0	8.625945953095275	0.0
10	0.0	0.0	0.0	11.20065778596826	0.0
11	0.0	0.0	0.0	12.489115890689375	0.0
12	0.0	0.0	0.0	15.464142508536447	0.0
13	0.0	0.0	0.0	16.003994330343463	0.0
14	0.0	0.0	0.0	16.261024638316915	0.0
15	0.0	0.0	0.0	16.789854577277726	0.0
16	0.0	0.0	0.0	17.542869613330307	0.0
17	0.0	0.0	0.0	18.33534298997229	0.0
18	0.0	0.0	0.0	19.250379703864052	0.0
19	0.0	0.0	0.0	19.788908899729524	0.0
20	0.0	0.0	0.0	20.330083347478084	0.0
21	0.0	0.0	0.0	20.91292051238529	0.0
22	0.0	0.0	0.0	21.583050989434422	0.0
23	0.0	0.0	0.0	22.194324612084834	0.0
24	0.0	0.0	0.0	22.66297507070538	0.0
25	0.0	0.0	0.0	23.115533580370467	0.0
26	0.0	0.0	0.0	23.513203113461465	0.0
27	0.0	0.0	0.0	23.967304686725022	0.0
28	0.0	0.0	0.0	24.35086620977288	0.0
29	0.0	0.0	0.0	24.784026205628656	0.0
30	0.0	0.0	0.0	25.258848918643075	0.0
31	0.0	0.0	0.0	25.732128568059025	0.0
32	0.0	0.0	0.0	26.173444757220988	0.0
33	0.0	0.0	0.0	26.581915735501266	0.0
34	0.0	0.0	0.0	26.97870351796457	0.0
35	0.0	0.0	0.0	27.4380955949943	0.0
36	0.0	0.0	0.0	27.876105219302403	0.0
37	0.0	0.0	0.0	28.349384868718353	0.0
38	0.0	0.0	0.0	28.782324426917203	0.0
39	0.0	0.0	0.0	29.254722325705455	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTAACG	20	7.028767E-4	45.0	1
CGGGTCA	20	7.028767E-4	45.0	6
TACCCTT	25	3.8870596E-5	45.0	34
ATGATCG	20	7.028767E-4	45.0	27
GATCGAG	20	7.028767E-4	45.0	1
TTTACGA	20	7.028767E-4	45.0	38
GAATGCG	25	3.8870596E-5	45.0	1
GACAAGC	25	3.8870596E-5	45.0	20
GTTAGCG	25	3.8870596E-5	45.0	1
CGTTCCA	25	3.8870596E-5	45.0	23
TGTTGCG	20	7.028767E-4	45.0	26
ACGATAA	20	7.028767E-4	45.0	35
TGCGCGA	25	3.8870596E-5	45.0	27
ATTTTCG	25	3.8870596E-5	45.0	23
GTATGCG	20	7.028767E-4	45.0	1
TTGACGA	20	7.028767E-4	45.0	15
TGCTCGA	20	7.028767E-4	45.0	1
CTCGACT	20	7.028767E-4	45.0	20
TTACGAG	20	7.028767E-4	45.0	1
CACCGTT	20	7.028767E-4	45.0	36
>>END_MODULE