##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936754.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	455156
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.900018894620747	31.0	31.0	34.0	30.0	34.0
2	31.962696306321348	33.0	31.0	34.0	30.0	34.0
3	31.926572427914824	33.0	31.0	34.0	30.0	34.0
4	35.63937419258452	37.0	35.0	37.0	33.0	37.0
5	30.830625104359825	37.0	35.0	37.0	0.0	37.0
6	33.131286855495695	37.0	35.0	37.0	17.0	37.0
7	35.00685039854468	37.0	35.0	37.0	32.0	37.0
8	35.3186467936268	37.0	35.0	37.0	33.0	37.0
9	37.41669449595304	39.0	37.0	39.0	35.0	39.0
10	37.28100914851172	39.0	37.0	39.0	34.0	39.0
11	37.00469948764819	39.0	37.0	39.0	33.0	39.0
12	36.79443092038773	39.0	35.0	39.0	33.0	39.0
13	36.517141375704156	39.0	35.0	39.0	32.0	39.0
14	37.79385968766753	40.0	36.0	41.0	33.0	41.0
15	37.68793995904701	40.0	36.0	41.0	33.0	41.0
16	37.8128795402016	40.0	36.0	41.0	33.0	41.0
17	37.673478983029995	40.0	36.0	41.0	33.0	41.0
18	37.55944774978249	39.0	36.0	41.0	32.0	41.0
19	37.49188410127517	39.0	36.0	41.0	32.0	41.0
20	37.561649192804225	39.0	35.0	41.0	32.0	41.0
21	37.64440543462022	39.0	36.0	41.0	33.0	41.0
22	37.653714330910724	39.0	36.0	41.0	33.0	41.0
23	37.72406164040461	39.0	36.0	41.0	33.0	41.0
24	37.59047227763668	39.0	35.0	41.0	33.0	41.0
25	37.54885577692044	39.0	35.0	41.0	33.0	41.0
26	37.36900535201118	39.0	35.0	41.0	33.0	41.0
27	37.41110740053959	39.0	35.0	41.0	33.0	41.0
28	37.34757313975867	39.0	35.0	41.0	33.0	41.0
29	37.32856427246922	39.0	35.0	41.0	32.0	41.0
30	37.313565019465855	39.0	35.0	41.0	32.0	41.0
31	37.24920027419171	39.0	35.0	41.0	32.0	41.0
32	37.119870989287186	39.0	35.0	41.0	32.0	41.0
33	37.056290590478866	39.0	35.0	41.0	32.0	41.0
34	36.941754035978875	39.0	35.0	41.0	31.0	41.0
35	36.9348399230154	39.0	35.0	41.0	31.0	41.0
36	36.83336482436791	39.0	35.0	41.0	31.0	41.0
37	36.69523416147431	39.0	35.0	40.0	31.0	41.0
38	36.66102610972941	39.0	35.0	40.0	31.0	41.0
39	36.55079796816916	39.0	35.0	40.0	31.0	41.0
40	36.437518125653625	39.0	35.0	40.0	30.0	41.0
41	36.330106600813785	38.0	35.0	40.0	30.0	41.0
42	36.318917030644435	38.0	35.0	40.0	30.0	41.0
43	36.298877747409676	38.0	35.0	40.0	30.0	41.0
44	36.18793995904701	38.0	35.0	40.0	30.0	41.0
45	36.19004253486717	38.0	35.0	40.0	30.0	41.0
46	35.985985024914534	38.0	35.0	40.0	30.0	41.0
47	35.75542011969522	38.0	35.0	40.0	29.0	41.0
48	35.71222174375379	38.0	35.0	40.0	29.0	41.0
49	35.60081598397033	38.0	35.0	40.0	28.0	41.0
50	35.40342651750169	38.0	34.0	40.0	28.0	41.0
51	33.47127797941805	36.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	10.0
9	27.0
10	32.0
11	23.0
12	5.0
13	10.0
14	10.0
15	22.0
16	35.0
17	66.0
18	164.0
19	307.0
20	471.0
21	712.0
22	980.0
23	1380.0
24	1881.0
25	2636.0
26	3282.0
27	4262.0
28	5165.0
29	6448.0
30	8762.0
31	11573.0
32	16016.0
33	23334.0
34	35640.0
35	44596.0
36	39506.0
37	57481.0
38	84267.0
39	106029.0
40	24.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	52.08609795322923	17.937586234170265	20.87064654755732	9.105669265043193
2	28.452442678993577	34.16059548814033	21.383437766392184	16.00352406647391
3	29.143853975340324	34.51344154531633	22.565889497227325	13.776814982116022
4	26.696341474131945	22.139002891316384	34.18058863334769	16.984067001203982
5	27.20715534893531	36.16474351650862	18.653824183356914	17.97427695119915
6	24.42964609935934	38.40595312376416	23.371767042508505	13.792633734367996
7	71.0224626281978	5.6334531457346495	17.66800833120952	5.676075894858027
8	67.88002355236446	12.960611306892583	12.267442371406727	6.891922769336227
9	62.998400548383415	6.924439093409732	11.947552048089007	18.129608310117852
10	43.813549640123384	19.49661215055937	20.669836275914193	16.02000193340305
11	35.27559781701219	21.63565898285423	23.150743920765628	19.93799927936795
12	30.65322658604962	19.701596815157878	28.670829342027787	20.974347256764712
13	23.60926803118052	22.84842998883899	35.04446827021944	18.49783370976105
14	21.19976447635536	29.8231815026057	25.581339145260085	23.395714875778854
15	17.272759229802528	23.84017787308088	41.72855021135611	17.158512685760485
16	19.45289087697405	25.143906704514496	30.2393025687896	25.163899849721854
17	19.266361423336175	22.783397340691984	32.239495909094906	25.71074532687694
18	19.99446343671181	26.934940987265904	29.649834342511138	23.420761233511147
19	23.031883573983425	27.65008041199061	25.8342194763993	23.48381653762666
20	26.031734174656602	26.541449524998022	30.340366819288334	17.086449481057045
21	23.585539902802555	30.625104359823887	26.376011741029448	19.41334399634411
22	19.609101055462304	23.908725799506104	28.17737215372312	28.304800991308475
23	20.384000210916696	30.437696086616455	28.748165464148556	20.43013823831829
24	25.59144557030996	21.451326578140243	25.603089929606554	27.354137921943245
25	20.33698336394555	31.10384132033852	24.823137561627224	23.736037754088706
26	19.351167511798153	25.3491110740054	27.138387717617697	28.161333696578755
27	23.069672815474256	28.627327773334855	25.37371802195291	22.92928138923798
28	18.241657805236006	25.565300688115723	31.88774837638085	24.305293130267426
29	23.456353426078092	23.654746943904946	25.314617405900396	27.574282224116565
30	20.987309845415638	25.190703846593255	33.04779020819236	20.77419609979875
31	28.667314063749572	24.389879513837016	22.48921248978372	24.45359393262969
32	25.80719577463551	31.033975164558964	23.228958862455947	19.92987019834958
33	23.87005773844572	23.19885929219872	27.741917057009026	25.189165912346535
34	23.6479360922409	28.13914350244751	24.829289298614103	23.383631106697482
35	19.506498870716854	23.520507254655545	27.517378656987933	29.455615217639668
36	27.351501463234584	25.361414547979155	24.099649350991747	23.187434637794517
37	21.451326578140243	29.57447556442187	29.884918577366882	19.089279280071008
38	25.148520507254656	25.218386663034213	24.63507017374263	24.9980226559685
39	25.126110608231023	29.216136884936155	24.76755222385292	20.8902002829799
40	21.434629006318712	21.112102224292332	32.964082644192324	24.489186125196635
41	21.388710683809506	29.632477656012448	24.55927198586858	24.41953967430947
42	21.428477269331832	22.24270360052378	29.660819587130565	26.667999543013828
43	28.557681322447685	22.31608503458155	24.115468103243725	25.010765539727036
44	22.805807239715612	22.9787149900254	28.97094622503054	25.244531545228448
45	20.477594495074218	21.820430797352998	26.714796685092583	30.987178022480204
46	27.023701763790875	26.191020221638293	24.333854766277934	22.451423248292894
47	18.69095431017058	22.54260077863414	36.28975560027771	22.476689310917575
48	22.653112339505576	23.312007311778817	26.345253056095054	27.68962729262055
49	20.246464948281467	19.852753781121198	34.990201161799476	24.910580108797863
50	21.357512589090334	21.458357134696676	30.06046278638533	27.123667489827664
51	19.295362469131465	21.870084103032806	25.67141815113939	33.163135276696345
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	121.0
1	346.0
2	571.0
3	877.5
4	1184.0
5	883.0
6	582.0
7	538.5
8	495.0
9	492.5
10	490.0
11	493.5
12	497.0
13	484.0
14	471.0
15	481.5
16	492.0
17	571.5
18	651.0
19	683.5
20	716.0
21	910.0
22	1104.0
23	1234.0
24	1364.0
25	1610.0
26	2346.5
27	2837.0
28	3518.5
29	4200.0
30	4822.0
31	5444.0
32	6367.0
33	7290.0
34	8221.5
35	9153.0
36	9522.0
37	9891.0
38	11647.0
39	13403.0
40	15593.5
41	17784.0
42	20742.0
43	23700.0
44	30597.5
45	37495.0
46	60090.0
47	82685.0
48	62938.0
49	43191.0
50	41959.5
51	40728.0
52	34184.5
53	27641.0
54	24024.5
55	20408.0
56	19981.0
57	19554.0
58	19010.5
59	18467.0
60	19454.5
61	20442.0
62	17920.0
63	15398.0
64	12698.5
65	9999.0
66	7863.5
67	5728.0
68	4631.5
69	3535.0
70	2861.0
71	2187.0
72	1946.0
73	1705.0
74	1261.0
75	626.5
76	436.0
77	334.5
78	233.0
79	154.0
80	75.0
81	55.5
82	36.0
83	36.5
84	37.0
85	31.0
86	25.0
87	25.5
88	26.0
89	17.0
90	8.0
91	4.5
92	1.0
93	1.0
94	1.0
95	1.5
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	455156.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.644845765821984
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.02178758317022	19.910312095155337
2	10.91123646957353	6.0327889863154445
3	4.244642241735265	3.5202744031159283
4	2.379651931348357	2.63140442473863
5	1.4528924907613272	2.008249441070892
6	1.0010574142953848	1.6604446692557082
7	0.7250838948175594	1.4031382707659048
8	0.5632915484410721	1.2457686382275588
9	0.4724540737433587	1.1754828000062494
>10	5.694901716225738	37.503280216413835
>50	0.46926363907047497	7.811824546512625
>100	0.05098959745417725	2.4286959872108578
>500	0.003983562301107598	0.6909240329990443
>1k	0.006373699681772156	3.6709055970656905
>5k	0.0	0.0
>10k+	0.0023901373806645585	8.306505891146305
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGC	13522	2.970849554877888	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCCGTCTTC	12731	2.7970629849985498	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCCG	11461	2.5180377716650995	No Hit
GCTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCCGTCTTCTGC	3430	0.753587780892705	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCCGTCT	2768	0.608143142131489	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCCGTC	1930	0.4240304423098894	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGC	1871	0.41106785365896525	No Hit
GAATATGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCCGTCTTC	1808	0.39722644543848706	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCCG	1787	0.39261264269832763	No Hit
GAACTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCCGTCTTCT	1784	0.39195352802116196	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTGATACGTCGTA	1289	0.28319960628883284	No Hit
CTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCCGTCTTCTGCT	718	0.15774811273497438	No Hit
CCTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCCGTCTTCTGC	701	0.15401312956436913	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCCGTCTT	596	0.13094411586357205	No Hit
GCTGACTCTTATACACATCTGACGCCTGATACGTCGTATGCCGTCTTCTGC	566	0.12435296909191573	No Hit
GAGGAGCTGAGAACCCCAGTCTAATAATGTCCATCGACACCTCCTTATCCC	556	0.1221559201680303	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	495	0.10875392173232913	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTGATACGTCGTATGCCGTCTTT	481	0.10567805323888954	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.19465853465624972	0.0
2	0.0	0.0	0.0	1.5135470036646776	0.0
3	0.0	0.0	0.0	1.839369359076888	0.0
4	0.0	0.0	0.0	2.6494212973134488	0.0
5	0.0	0.0	0.0	6.807995500443804	0.0
6	0.0	0.0	0.0	7.638699698564888	0.0
7	0.0	0.0	0.0	9.087433758974944	0.0
8	0.0	0.0	0.0	10.597685189253795	0.0
9	0.0	0.0	0.0	11.009412157589924	0.0
10	0.0	0.0	0.0	14.877316788090237	0.0
11	0.0	0.0	0.0	16.123263232825668	0.0
12	0.0	0.0	0.0	20.68235945478034	0.0
13	0.0	0.0	0.0	21.261062141331763	0.0
14	0.0	0.0	0.0	21.555246992240022	0.0
15	0.0	0.0	0.0	22.28027313712222	0.0
16	0.0	0.0	0.0	23.09164330471311	0.0
17	0.0	0.0	0.0	23.873792721616326	0.0
18	0.0	0.0	0.0	24.697466363180975	0.0
19	0.0	0.0	0.0	25.31044301294501	0.0
20	0.0	0.0	0.0	25.83114360790586	0.0
21	0.0	0.0	0.0	26.415998031444165	0.0
22	0.0	0.0	0.0	27.093348214678045	0.0
23	0.0	0.0	0.0	27.703908110625807	0.0
24	0.0	0.0	0.0	28.209668772904234	0.0
25	0.0	0.0	0.0	28.63457803478368	0.0
26	0.0	0.0	0.0	29.021258645387515	0.0
27	0.0	0.0	0.0	29.42837181098349	0.0
28	0.0	0.0	0.0	29.849546089692325	0.0
29	0.0	0.0	0.0	30.274015941786992	0.0
30	0.0	0.0	0.0	30.74044942832787	0.0
31	0.0	0.0	0.0	31.215890815456678	0.0
32	0.0	0.0	0.0	31.655300600233765	0.0
33	0.0	0.0	0.0	32.07867192786649	0.0
34	0.0	0.0	0.0	32.52621079366195	0.0
35	0.0	0.0	0.0	32.992424575310444	0.0
36	0.0	0.0	0.0	33.46896448690119	0.0
37	0.0	0.0	0.0	33.94989849633971	0.0
38	0.0	0.0	0.0	34.38689152730053	0.0
39	0.0	0.0	0.0	34.85266589916424	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GACCGTA	30	2.162602E-6	45.000004	6
CGCATGG	60	0.0	45.000004	2
GCTATGT	30	2.162602E-6	45.000004	10
ATAGCTA	30	2.162602E-6	45.000004	12
CGTTCAT	30	2.162602E-6	45.000004	17
CCGTACA	30	2.162602E-6	45.000004	8
TCATGCG	30	2.162602E-6	45.000004	1
ACCGTAC	30	2.162602E-6	45.000004	7
CGATCCC	30	2.162602E-6	45.000004	43
GTCATCG	20	7.028792E-4	45.0	25
CGAAAAA	25	3.88708E-5	45.0	22
ATTACGG	20	7.028792E-4	45.0	2
ATTACGA	20	7.028792E-4	45.0	36
TAAACGG	20	7.028792E-4	45.0	2
GCGCATG	50	2.1827873E-11	45.0	1
TGCATTA	20	7.028792E-4	45.0	16
CATTACG	20	7.028792E-4	45.0	35
CCCGTTC	20	7.028792E-4	45.0	17
TTCCCGG	25	3.88708E-5	45.0	11
ACGATAA	20	7.028792E-4	45.0	44
>>END_MODULE