##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936752.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	651496
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.00809674963469	33.0	31.0	34.0	30.0	34.0
2	32.090098788020185	33.0	31.0	34.0	30.0	34.0
3	32.04559813107064	33.0	31.0	34.0	30.0	34.0
4	35.728382062207594	37.0	35.0	37.0	33.0	37.0
5	31.025010130530347	37.0	35.0	37.0	0.0	37.0
6	33.3197487014502	37.0	35.0	37.0	17.0	37.0
7	35.246739811142355	37.0	35.0	37.0	32.0	37.0
8	35.65057651927257	37.0	35.0	37.0	35.0	37.0
9	37.623567911391625	39.0	37.0	39.0	35.0	39.0
10	37.31964432628903	39.0	37.0	39.0	34.0	39.0
11	37.15350669842946	39.0	37.0	39.0	34.0	39.0
12	37.067228348293774	39.0	37.0	39.0	34.0	39.0
13	36.91944079472476	39.0	37.0	39.0	33.0	39.0
14	38.11270675491484	40.0	37.0	41.0	33.0	41.0
15	38.19373257855766	40.0	37.0	41.0	33.0	41.0
16	38.18080080307477	40.0	37.0	41.0	34.0	41.0
17	38.15103853285362	40.0	37.0	41.0	34.0	41.0
18	38.072784790697106	40.0	37.0	41.0	33.0	41.0
19	38.04147684713337	40.0	37.0	41.0	34.0	41.0
20	38.04668025590334	40.0	37.0	41.0	34.0	41.0
21	37.98535217407321	40.0	36.0	41.0	33.0	41.0
22	37.98258162751575	40.0	36.0	41.0	34.0	41.0
23	38.00155488291563	40.0	36.0	41.0	34.0	41.0
24	37.95480862507214	40.0	36.0	41.0	34.0	41.0
25	37.80549535223547	40.0	36.0	41.0	33.0	41.0
26	37.75726788806071	40.0	36.0	41.0	33.0	41.0
27	37.71842804867566	40.0	36.0	41.0	33.0	41.0
28	37.61111042892052	40.0	36.0	41.0	33.0	41.0
29	37.56983312253644	40.0	36.0	41.0	33.0	41.0
30	37.475453417979544	40.0	36.0	41.0	33.0	41.0
31	37.36003751366086	40.0	35.0	41.0	32.0	41.0
32	37.29024736913197	39.0	35.0	41.0	32.0	41.0
33	37.23390627110527	39.0	35.0	41.0	32.0	41.0
34	37.087882350774215	39.0	35.0	41.0	31.0	41.0
35	37.0799314193794	39.0	35.0	41.0	31.0	41.0
36	36.922028684750174	39.0	35.0	41.0	31.0	41.0
37	36.88500773604136	39.0	35.0	41.0	31.0	41.0
38	36.79106241634638	39.0	35.0	41.0	31.0	41.0
39	36.77026720041259	39.0	35.0	41.0	31.0	41.0
40	36.728893193511546	39.0	35.0	41.0	31.0	41.0
41	36.65620510333141	39.0	35.0	41.0	31.0	41.0
42	36.59269435268981	39.0	35.0	41.0	31.0	41.0
43	36.508508110563994	39.0	35.0	41.0	30.0	41.0
44	36.36166914301853	39.0	35.0	40.0	30.0	41.0
45	36.33211101833319	39.0	35.0	40.0	30.0	41.0
46	36.17371403661726	38.0	35.0	40.0	30.0	41.0
47	36.08587926863711	38.0	35.0	40.0	29.0	41.0
48	35.9865248597075	38.0	35.0	40.0	29.0	41.0
49	35.855395888846594	38.0	35.0	40.0	29.0	41.0
50	35.675939683436276	38.0	35.0	40.0	28.0	41.0
51	33.91769558063288	36.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	19.0
9	41.0
10	46.0
11	27.0
12	24.0
13	15.0
14	16.0
15	31.0
16	69.0
17	97.0
18	169.0
19	307.0
20	566.0
21	924.0
22	1443.0
23	2001.0
24	2910.0
25	3719.0
26	4671.0
27	5664.0
28	6833.0
29	8710.0
30	10951.0
31	14556.0
32	19296.0
33	28127.0
34	45001.0
35	54743.0
36	54686.0
37	84069.0
38	129758.0
39	171970.0
40	33.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.31155371636971	22.008116703709614	22.590468705870794	11.08986087404988
2	31.043168338715816	26.17544850620725	24.53353512531159	18.24784802976534
3	33.58224762700001	25.399848963002075	25.329702714982133	15.68820069501578
4	30.1260483563982	25.648660928079376	25.945209180102413	18.280081535420017
5	22.89039380134337	39.413749278583445	20.41654898878888	17.279307931284304
6	26.88274371600133	35.9133440573695	23.547036359394376	13.656875867234794
7	79.40309687242899	6.694592138708449	9.150938762478972	4.751372226383585
8	78.84008497366062	6.863741296953474	8.641495880250991	5.654677849134914
9	72.09100286110736	8.859762761398382	10.998379115144221	8.050855262350037
10	40.9537433844567	24.572829303633483	19.198736446578337	15.274690865331483
11	31.59098444196127	27.266322433291997	22.858006802804624	18.284686321942115
12	29.71975269226519	22.80290285742353	28.11820793987991	19.359136510431377
13	25.14643221140268	25.245895600279972	30.05789751587116	19.549774672446183
14	20.21194297432371	29.403096872428996	28.457580706558446	21.927379446688853
15	19.964512445203038	26.545826835467906	34.57887692326584	18.910783796063217
16	21.94180777779142	25.97375885653941	31.267574935226	20.816858430443162
17	21.428220587693556	25.572988936232914	28.628418286528234	24.370372189545293
18	23.61871753625502	26.860794233579334	28.924045581246855	20.596442648918796
19	24.55026584967521	28.554127730638406	26.021034664832936	20.874571754853445
20	25.85664378599408	26.944601348281495	27.234856392057665	19.96389847366676
21	24.071061065608998	28.486437368763585	27.7169775409212	19.725524024706214
22	21.964064245981557	26.812904453749525	26.603079681225978	24.61995161904294
23	22.19061974286872	27.53048368677628	27.65911072362685	22.619785846728146
24	23.57236268526591	24.820259832754154	28.7647199675823	22.842657514397633
25	21.7395041565873	27.26785736213269	26.37928705625207	24.613351425027936
26	21.31816618981544	28.443612854108085	27.337543131500425	22.90067782457605
27	23.376045286540517	27.781137566462416	26.70438498471211	22.138432162284957
28	19.74946891462112	27.848367449685036	29.58114861795007	22.82101501774378
29	23.899916499871065	25.999545661063152	27.947063374141973	22.153474464923807
30	25.039140685437822	25.85971364367548	27.198785564301236	21.90236010658546
31	24.274592629885678	26.940150054643468	26.091948377273226	22.693308938197625
32	25.976521728452667	27.953049596620698	26.32142023895772	19.74900843596891
33	25.719267654751526	26.986351412748505	25.142441396416864	22.151939536083106
34	22.840355121136586	25.81504721441114	28.792041700946747	22.552555963505533
35	23.91802866019131	25.93646008571043	26.332778712378897	23.812732541719367
36	23.710659773812885	28.89917973402753	26.879366872551792	20.510793619607796
37	23.32370421307268	27.49886415265788	28.827345064282824	20.350086569986615
38	22.57020764517357	28.567021132900276	26.056338028169012	22.806433193757137
39	23.665225880128197	25.635460540049365	27.8770706190061	22.822242960816336
40	24.42977393568034	24.709284477571618	28.225959944496974	22.634981642251066
41	21.270122917101563	27.95351007527291	26.95365742844162	23.822709579183908
42	23.17926740916291	27.364711371980793	27.335547724007515	22.120473494848778
43	23.776201235310733	25.998010732222454	28.02826110981495	22.197526922651868
44	22.74810589781058	25.484116556356444	27.407842872404437	24.35993467342854
45	22.8948450949814	24.357171801515282	27.390498176504536	25.357484926998787
46	23.973593084224614	26.152578066480835	26.44114468853224	23.432684160762307
47	20.814095558529907	26.493639254884144	29.29734027530484	23.394924911281112
48	22.259077569163892	24.604755823520023	28.994038336382726	24.142128270933362
49	21.97081793288063	23.99984036740057	30.067567567567565	23.961774132151234
50	20.8804044844481	23.739209450249888	30.059125458943726	25.321260606358287
51	20.606880165035548	24.00751501160406	27.112215577685816	28.27338924567457
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	163.0
1	422.0
2	681.0
3	1085.0
4	1489.0
5	1072.0
6	655.0
7	643.5
8	632.0
9	656.0
10	680.0
11	746.5
12	813.0
13	822.5
14	832.0
15	884.5
16	937.0
17	988.0
18	1039.0
19	1165.5
20	1292.0
21	1875.5
22	2459.0
23	2674.0
24	2889.0
25	3318.0
26	4594.5
27	5442.0
28	6628.0
29	7814.0
30	9234.5
31	10655.0
32	12221.5
33	13788.0
34	15718.5
35	17649.0
36	19566.5
37	21484.0
38	22623.0
39	23762.0
40	27019.5
41	30277.0
42	34454.5
43	38632.0
44	42849.5
45	47067.0
46	55771.0
47	64475.0
48	66688.5
49	68902.0
50	66221.0
51	63540.0
52	55495.5
53	47451.0
54	41232.0
55	35013.0
56	31953.5
57	28894.0
58	27463.0
59	26032.0
60	26187.5
61	26343.0
62	23558.0
63	20773.0
64	17097.0
65	13421.0
66	10465.5
67	7510.0
68	6288.0
69	5066.0
70	4078.5
71	3091.0
72	2837.0
73	2583.0
74	1966.5
75	1112.0
76	874.0
77	698.5
78	523.0
79	421.5
80	320.0
81	281.5
82	243.0
83	189.0
84	135.0
85	78.0
86	21.0
87	23.0
88	25.0
89	18.0
90	11.0
91	10.0
92	9.0
93	8.0
94	7.0
95	5.0
96	3.0
97	2.5
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	651496.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.06834672373868
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.50910657779261	22.216697170636433
2	11.182731704083558	6.9485797180202615
3	4.406290208916674	4.106884559281146
4	2.347267314520772	2.917028591233213
5	1.4493447543626519	2.251437267538537
6	1.0583797433198154	1.9729265298504945
7	0.79572929828643	1.730539561618006
8	0.614718369388627	1.5278626750093707
9	0.48784213852329106	1.3640803835482584
>10	5.619741111855849	41.377191331224275
>50	0.47365583238017	8.970499632193123
>100	0.05117891409173487	2.7994131965316105
>500	0.0010035081194457818	0.18675225578822083
>1k	0.003010524358337345	1.630107127527066
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTTGCAATCGTATGC	2143	0.32893525056178397	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTTGCAATCGTATGCCGTCTTC	2121	0.32555840711224626	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTTGCAATCGTATGCCG	2039	0.3129719906185149	No Hit
GCTGTCTCTTATACACATCTGACGCGGTTGCAATCGTATGCCGTCTTCTGC	1986	0.3048368677628105	No Hit
CCTGTCTCTTATACACATCTGACGCGGTTGCAATCGTATGCCGTCTTCTGC	1151	0.1766703095644486	No Hit
CTGTCTCTTATACACATCTGACGCGGTTGCAATCGTATGCCGTCTTCTGCT	1017	0.15610226309908273	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	655	0.10053783906578091	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.0698576813978904E-4	0.0	0.0	0.18081461743433574	0.0
2	3.0698576813978904E-4	0.0	0.0	0.9832754153517443	0.0
3	3.0698576813978904E-4	0.0	0.0	1.3522723086557706	0.0
4	3.0698576813978904E-4	0.0	0.0	1.8436030305635032	0.0
5	3.0698576813978904E-4	0.0	0.0	3.4654088436460087	0.0
6	3.0698576813978904E-4	0.0	0.0	4.3463045053231335	0.0
7	3.0698576813978904E-4	0.0	0.0	5.299955794049388	0.0
8	4.6047865220968356E-4	0.0	0.0	6.8353451134005425	0.0
9	4.6047865220968356E-4	0.0	0.0	7.373184179181453	0.0
10	4.6047865220968356E-4	0.0	0.0	8.909187470068888	0.0
11	4.6047865220968356E-4	0.0	0.0	10.468982157987156	0.0
12	4.6047865220968356E-4	0.0	0.0	12.022944116310768	0.0
13	4.6047865220968356E-4	0.0	0.0	12.615426648820561	0.0
14	4.6047865220968356E-4	0.0	0.0	12.886034603435785	0.0
15	4.6047865220968356E-4	0.0	0.0	13.359253165023269	0.0
16	4.6047865220968356E-4	0.0	0.0	14.258107494136572	0.0
17	4.6047865220968356E-4	0.0	0.0	15.227722042806095	0.0
18	4.6047865220968356E-4	0.0	0.0	16.368020678561344	0.0
19	4.6047865220968356E-4	0.0	0.0	16.99826246055233	0.0
20	4.6047865220968356E-4	0.0	0.0	17.6214435698761	0.0
21	4.6047865220968356E-4	0.0	0.0	18.390903397718482	0.0
22	4.6047865220968356E-4	0.0	0.0	19.169879784373197	0.0
23	4.6047865220968356E-4	0.0	0.0	19.956837800999544	0.0
24	4.6047865220968356E-4	0.0	0.0	20.57925144590297	0.0
25	4.6047865220968356E-4	0.0	0.0	21.12860247798912	0.0
26	4.6047865220968356E-4	0.0	0.0	21.647408426145365	0.0
27	4.6047865220968356E-4	0.0	0.0	22.14825570686543	0.0
28	4.6047865220968356E-4	0.0	0.0	22.665833711949116	0.0
29	4.6047865220968356E-4	0.0	0.0	23.222398909586552	0.0
30	4.6047865220968356E-4	0.0	0.0	23.818104792661813	0.0
31	4.6047865220968356E-4	0.0	0.0	24.37282807569041	0.0
32	4.6047865220968356E-4	0.0	0.0	24.94105873251716	0.0
33	4.6047865220968356E-4	0.0	0.0	25.456487837223865	0.0
34	4.6047865220968356E-4	0.0	0.0	26.0033829831649	0.0
35	4.6047865220968356E-4	0.0	0.0	26.52971008264057	0.0
36	4.6047865220968356E-4	0.0	0.0	27.09686628927883	0.0
37	4.6047865220968356E-4	0.0	0.0	27.639617127349975	0.0
38	4.6047865220968356E-4	0.0	0.0	28.178684136203447	0.0
39	4.6047865220968356E-4	0.0	0.0	28.767022360843352	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATACCGG	35	1.2108467E-7	45.000004	2
TCCGCCA	20	7.031034E-4	45.0	23
CGTGCCG	20	7.031034E-4	45.0	29
CGGTTAC	20	7.031034E-4	45.0	45
TAGCGCG	20	7.031034E-4	45.0	1
TAGCCGC	25	3.8889382E-5	45.0	43
AATTGCG	20	7.031034E-4	45.0	1
AACGCCG	20	7.031034E-4	45.0	13
GACGGTC	25	3.8889382E-5	45.0	29
CGTACAC	20	7.031034E-4	45.0	11
TGGATCG	20	7.031034E-4	45.0	2
CGGTCAT	25	3.8889382E-5	45.0	31
ATCGGTC	20	7.031034E-4	45.0	19
TATCGTC	20	7.031034E-4	45.0	43
TAACTCG	20	7.031034E-4	45.0	36
TGCGTCC	25	3.8889382E-5	45.0	18
ACGGTCA	25	3.8889382E-5	45.0	30
CGAAAGC	30	2.164048E-6	44.999996	23
TAACGGG	220	0.0	41.93182	3
TTGAGCG	50	1.0804797E-9	40.5	1
>>END_MODULE