##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936751.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	911233
Sequences flagged as poor quality	0
Sequence length	51
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.93312248349215	33.0	31.0	34.0	30.0	34.0
2	32.01227786965573	33.0	31.0	34.0	30.0	34.0
3	31.97220469407934	33.0	31.0	34.0	30.0	34.0
4	35.68303715954097	37.0	35.0	37.0	33.0	37.0
5	31.01755313953731	37.0	35.0	37.0	0.0	37.0
6	33.24541363185925	37.0	35.0	37.0	17.0	37.0
7	35.1087230159575	37.0	35.0	37.0	32.0	37.0
8	35.46824467507213	37.0	35.0	37.0	33.0	37.0
9	37.502775909125326	39.0	37.0	39.0	35.0	39.0
10	37.29942835696249	39.0	37.0	39.0	34.0	39.0
11	37.029213165019264	39.0	37.0	39.0	33.0	39.0
12	36.79471660925361	39.0	35.0	39.0	33.0	39.0
13	36.51160570348089	39.0	35.0	39.0	32.0	39.0
14	37.41024194690052	40.0	36.0	41.0	32.0	41.0
15	37.49536287645421	40.0	36.0	41.0	32.0	41.0
16	37.673590618425806	40.0	36.0	41.0	33.0	41.0
17	37.65098608149617	40.0	36.0	41.0	33.0	41.0
18	37.570692676845546	39.0	36.0	41.0	33.0	41.0
19	37.52020284603389	39.0	36.0	41.0	33.0	41.0
20	37.481598010607605	39.0	35.0	41.0	32.0	41.0
21	37.47711507375172	39.0	35.0	41.0	33.0	41.0
22	37.56675954448533	39.0	35.0	41.0	33.0	41.0
23	37.62028153062938	39.0	35.0	41.0	33.0	41.0
24	37.52659528353341	39.0	35.0	41.0	33.0	41.0
25	37.412361053649285	39.0	35.0	41.0	33.0	41.0
26	37.32101778579134	39.0	35.0	41.0	33.0	41.0
27	37.30482105015951	39.0	35.0	41.0	33.0	41.0
28	37.26498052638568	39.0	35.0	41.0	32.0	41.0
29	37.161491078571565	39.0	35.0	41.0	32.0	41.0
30	37.09086260045455	39.0	35.0	41.0	32.0	41.0
31	36.978533481557406	39.0	35.0	41.0	31.0	41.0
32	36.782991836336045	39.0	35.0	41.0	31.0	41.0
33	36.74893688002959	39.0	35.0	41.0	31.0	41.0
34	36.486372859630855	39.0	35.0	41.0	30.0	41.0
35	36.42421312660977	39.0	35.0	41.0	30.0	41.0
36	36.28868357489248	39.0	35.0	40.0	30.0	41.0
37	36.16684536227287	38.0	35.0	40.0	30.0	41.0
38	36.01577423117907	38.0	35.0	40.0	29.0	41.0
39	35.59694721328135	38.0	34.0	40.0	27.0	41.0
40	35.427973964946396	38.0	34.0	40.0	26.0	41.0
41	35.193561910071296	38.0	34.0	40.0	25.0	41.0
42	35.28881855683453	38.0	34.0	40.0	26.0	41.0
43	35.25599489921897	38.0	34.0	40.0	26.0	41.0
44	35.1211885434351	38.0	34.0	40.0	26.0	41.0
45	35.101317665185526	38.0	34.0	40.0	26.0	41.0
46	34.835537123875014	37.0	34.0	40.0	24.0	41.0
47	34.62337404374073	37.0	34.0	40.0	23.0	41.0
48	34.476466501981385	37.0	33.0	40.0	23.0	41.0
49	34.372235202193075	37.0	33.0	40.0	23.0	41.0
50	34.17988703218606	37.0	33.0	40.0	23.0	41.0
51	32.128729973563296	35.0	30.0	39.0	17.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	7.0
8	29.0
9	47.0
10	80.0
11	39.0
12	33.0
13	18.0
14	24.0
15	67.0
16	120.0
17	233.0
18	416.0
19	744.0
20	1249.0
21	1774.0
22	2730.0
23	3910.0
24	5512.0
25	7636.0
26	10201.0
27	12483.0
28	14953.0
29	17550.0
30	21803.0
31	26843.0
32	35314.0
33	48919.0
34	72465.0
35	76968.0
36	77590.0
37	112930.0
38	161103.0
39	197417.0
40	26.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	47.228645143448496	19.61375411118781	22.45276455088874	10.704836194474959
2	29.846263249904247	30.146076799237953	23.316868462841008	16.69079148801679
3	31.069111851743735	29.33508773277526	24.414831332930216	15.180969082550785
4	28.37342370173161	23.004654133465316	30.88441704810954	17.737505116693537
5	25.246781009906353	36.4613660830984	20.736079575695786	17.55577333129946
6	25.55065499164319	36.08813552625947	24.930067282462335	13.431142199635
7	76.12641333226519	5.618431290350547	13.506863776882533	4.748291600501737
8	74.91519732055359	9.454332755727679	10.200135420907715	5.430334502811027
9	69.12776424909984	6.4272255284872255	11.504302412226073	12.940707810186858
10	45.44765169830329	19.384175068286595	19.47130975282941	15.696863480580708
11	34.115204344004226	22.786268715026782	23.870404166662095	19.2281227743069
12	31.900293338805774	18.84501549000091	28.388348534348513	20.866342636844802
13	25.974695824229364	20.984534142200733	34.43433238260686	18.606437650963038
14	21.875085735481484	25.48634652169094	30.907243262700103	21.731324480127476
15	18.737249419193557	22.89546142424605	39.81177152276092	18.55551763379948
16	24.133893307200243	24.614999676262823	27.791574712504925	23.45953230403201
17	24.718156607585545	22.727666798722172	28.915546298257418	23.63863029543487
18	24.821533021740873	24.666907366173085	29.482580196283497	21.028979415802546
19	24.683588061450802	26.65531208812675	25.90972890577931	22.75137094464314
20	24.06047629969503	28.677297683468446	29.069294022494795	18.192931994341734
21	23.048989665650826	27.861699477521118	30.941482584585938	18.147828272242116
22	21.484406293450743	22.013030695771555	30.855006348540932	25.64755666223677
23	20.909909979116208	26.996717634238443	31.040798566338136	21.05257382030721
24	23.831336222459022	24.174936596896732	26.944041754414073	25.049685426230173
25	22.814472259016082	29.470837864739313	24.92106848632567	22.793621389918933
26	20.33738901027509	23.995509381244972	26.9900234078441	28.67707820063584
27	22.320855368495216	25.778917137548795	26.663871918598208	25.236355575357784
28	18.43348517887302	24.429646424130823	29.274181246728332	27.862687150267824
29	22.964269292266632	23.657286336206	25.94561434890966	27.432830022617704
30	22.981388953209553	23.769112839416483	27.956625802621282	25.292872404752682
31	23.936578240691457	22.381652113125842	26.10155690147306	27.58021274470964
32	26.009703336029315	23.893120639836354	27.02294583273433	23.074230191400005
33	23.665297459595955	22.10246995005668	27.061574811272198	27.170657779075164
34	24.722656005653878	21.539057518768526	29.326418160887503	24.411868314690096
35	20.34024228709891	23.538985089433766	29.311712811103195	26.80905981236413
36	23.47994420746395	25.73589850235889	24.763260329685163	26.02089696049199
37	20.906727478043486	24.77884361079987	30.30179986896875	24.012629042187893
38	22.051549932893124	24.12357761406797	27.655166132043068	26.16970632099584
39	25.066256380091588	22.190263083097296	29.19560639265698	23.54787414415413
40	24.20862721170107	20.43110817979595	33.682054973865085	21.678209634637902
41	22.417537556256192	25.28025214187809	26.404113986214284	25.89809631565143
42	19.894033688419977	22.221319903910416	34.11553356825313	23.769112839416483
43	22.184227305200753	24.09372794883416	28.234161844445932	25.48788290151915
44	22.735787663528427	21.533350965120885	29.035713149106762	26.695148222243926
45	20.57377202098695	20.323232367572288	28.13583353544044	30.96716207600032
46	25.00556937687726	23.38413995103338	26.867222763003536	24.743067909085823
47	18.801448147729506	22.372214351323976	34.746656453398856	24.079681047547663
48	20.944698008083552	24.988449715934344	27.690502868091915	26.37634940789019
49	22.01533526551387	20.67089317441313	32.74233922608158	24.571432333991417
50	21.896485311660136	21.42404851448532	29.64675335506945	27.0327128187851
51	20.609108757035795	22.017420352423585	26.408942608531515	30.964528282009102
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	242.0
1	582.5
2	923.0
3	1866.0
4	2809.0
5	1971.5
6	1134.0
7	1045.5
8	957.0
9	970.5
10	984.0
11	995.0
12	1006.0
13	1081.0
14	1156.0
15	1094.0
16	1032.0
17	1123.0
18	1214.0
19	1342.0
20	1470.0
21	1778.0
22	2086.0
23	2516.0
24	2946.0
25	3485.0
26	4984.5
27	5945.0
28	7252.0
29	8559.0
30	10090.5
31	11622.0
32	13341.0
33	15060.0
34	17387.0
35	19714.0
36	21681.5
37	23649.0
38	25981.5
39	28314.0
40	31918.0
41	35522.0
42	39509.5
43	43497.0
44	53881.0
45	64265.0
46	92205.5
47	120146.0
48	102426.5
49	84707.0
50	83847.0
51	82987.0
52	73119.0
53	63251.0
54	57410.5
55	51570.0
56	49492.0
57	47414.0
58	46172.5
59	44931.0
60	40860.5
61	36790.0
62	33091.0
63	29392.0
64	25761.5
65	22131.0
66	18844.0
67	15557.0
68	13598.5
69	11640.0
70	10158.0
71	8676.0
72	7137.0
73	5598.0
74	4721.0
75	2963.0
76	2082.0
77	1592.5
78	1103.0
79	832.0
80	561.0
81	494.0
82	427.0
83	287.0
84	147.0
85	110.5
86	74.0
87	53.0
88	32.0
89	27.0
90	22.0
91	12.5
92	3.0
93	4.0
94	5.0
95	8.0
96	11.0
97	6.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	911233.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.954052034968004
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.53794202512927	22.027593419651822
2	10.199659225872141	6.110422463814312
3	3.9389650274739214	3.5396389019061907
4	2.1904601201777347	2.6245262568130454
5	1.4226276996204397	2.130673207040874
6	0.9943641364697522	1.787114105131259
7	0.7515455574394403	1.575828431393302
8	0.5495069836578017	1.3167968625651283
9	0.45499110431226525	1.2265944492615433
>10	5.4907778105968585	40.455279842438216
>50	0.41711459503279885	7.397753150132577
>100	0.04493155184250297	2.458814333718485
>500	0.0018721479934376193	0.4550202869997665
>1k	0.0041187255855627625	2.368191609563734
>5k	0.0	0.0
>10k+	0.0011232887960625716	4.5257526795697745
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGC	14360	1.5758867380790642	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTC	13114	1.4391489333683043	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCG	12878	1.4132499591213223	No Hit
GCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGC	3259	0.35764727572421107	TruSeq Adapter, Index 14 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCT	2554	0.28027957723216784	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGC	2203	0.24176034011059738	No Hit
GAATATGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTC	2180	0.2392362875356797	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCG	2173	0.23846809762157428	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTC	1899	0.20839894955516314	No Hit
GAACTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCT	1696	0.18612144204610676	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	1387	0.15221134440916867	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1344	0.14749246350823556	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCCCTTGTATCGTA	1332	0.1461755665126263	No Hit
CCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTCTGC	1088	0.1193986609352383	TruSeq Adapter, Index 14 (95% over 21bp)
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCTGTCTCTTATACACATCTGAC	954	0.10469331115093505	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTT	948	0.10403486265313042	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.19482832601541E-4	0.0	0.0	0.13772547745746697	0.0
2	2.19482832601541E-4	0.0	0.0	1.0249848282491965	0.0
3	2.19482832601541E-4	0.0	0.0	1.3091053550518912	0.0
4	2.19482832601541E-4	0.0	0.0	1.8919420170252834	0.0
5	2.19482832601541E-4	0.0	0.0	4.796138858008874	0.0
6	2.19482832601541E-4	0.0	0.0	5.669680531763007	0.0
7	2.19482832601541E-4	0.0	0.0	6.876616628238881	0.0
8	2.19482832601541E-4	0.0	0.0	8.37908635881273	0.0
9	2.19482832601541E-4	0.0	0.0	8.875227301908513	0.0
10	2.19482832601541E-4	0.0	0.0	11.69624014933612	0.0
11	2.19482832601541E-4	0.0	0.0	13.006333177134717	0.0
12	2.19482832601541E-4	0.0	0.0	16.08732343977885	0.0
13	2.19482832601541E-4	0.0	0.0	16.6073880116282	0.0
14	2.19482832601541E-4	0.0	0.0	16.85672050946355	0.0
15	2.19482832601541E-4	0.0	0.0	17.380516289467128	0.0
16	2.19482832601541E-4	0.0	0.0	18.070680056582674	0.0
17	2.19482832601541E-4	0.0	0.0	18.777305036143336	0.0
18	2.19482832601541E-4	0.0	0.0	19.549555382651857	0.0
19	2.19482832601541E-4	0.0	0.0	20.052061327893085	0.0
20	2.19482832601541E-4	0.0	0.0	20.57256486540764	0.0
21	2.19482832601541E-4	0.0	0.0	21.16900946300233	0.0
22	2.19482832601541E-4	0.0	0.0	21.80869217861952	0.0
23	2.19482832601541E-4	0.0	0.0	22.39580875582864	0.0
24	2.19482832601541E-4	0.0	0.0	22.862429257939517	0.0
25	2.19482832601541E-4	0.0	0.0	23.283397330869274	0.0
26	2.19482832601541E-4	0.0	0.0	23.68735548427241	0.0
27	3.292242489023115E-4	0.0	0.0	24.25043869131166	0.0
28	3.292242489023115E-4	0.0	0.0	24.678100990635766	0.0
29	3.292242489023115E-4	0.0	0.0	25.171937363989233	0.0
30	3.292242489023115E-4	0.0	0.0	25.768162478751318	0.0
31	3.292242489023115E-4	0.0	0.0	26.227979013051545	0.0
32	4.38965665203082E-4	0.0	0.0	26.653336742633332	0.0
33	4.38965665203082E-4	0.0	0.0	27.088680941098488	0.0
34	4.38965665203082E-4	0.0	0.0	27.56858015458176	0.0
35	4.38965665203082E-4	0.0	0.0	28.083267397032373	0.0
36	5.487070815038524E-4	0.0	0.0	28.5433034141652	0.0
37	5.487070815038524E-4	0.0	0.0	29.046358066487933	0.0
38	5.487070815038524E-4	0.0	0.0	29.627987572882017	0.0
39	5.487070815038524E-4	0.0	0.0	30.74350906957935	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGAACTA	20	7.032515E-4	45.000004	34
ACCGCGT	20	7.032515E-4	45.000004	21
TACGGGT	70	0.0	45.000004	4
CGCGAAC	25	3.8901664E-5	45.000004	43
GCGATAT	50	2.1827873E-11	45.000004	9
TTGCACG	20	7.032515E-4	45.000004	1
CGCGTAA	25	3.8901664E-5	45.000004	31
TCGAATA	25	3.8901664E-5	45.000004	39
GCGTAAC	25	3.8901664E-5	45.000004	32
TAGTACG	30	2.1650048E-6	45.000004	1
ACGGGTC	75	0.0	45.0	5
TAATACG	405	0.0	43.88889	4
TACGGCT	1985	0.0	43.299747	7
TAGCCGT	85	0.0	42.35294	44
TTAACGG	60	3.6379788E-12	41.250004	2
ACGGCTG	2095	0.0	41.026253	8
TCGGGTA	55	6.002665E-11	40.909092	5
TCGGCAT	50	1.0804797E-9	40.500004	32
TAACGGG	235	0.0	40.212765	3
TCGTTTA	45	1.927583E-8	40.000004	38
>>END_MODULE