##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936749.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	747274
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.05295380275508	33.0	31.0	34.0	30.0	34.0
2	32.15022869790733	33.0	31.0	34.0	30.0	34.0
3	32.06646959482064	33.0	31.0	34.0	30.0	34.0
4	35.77456461752985	37.0	35.0	37.0	35.0	37.0
5	30.9445852525312	37.0	35.0	37.0	0.0	37.0
6	33.301252284971774	37.0	35.0	37.0	17.0	37.0
7	35.31846417779824	37.0	35.0	37.0	32.0	37.0
8	35.770298444747176	37.0	35.0	37.0	35.0	37.0
9	37.783360587950334	39.0	37.0	39.0	35.0	39.0
10	37.45657817614423	39.0	37.0	39.0	35.0	39.0
11	37.251836675703956	39.0	37.0	39.0	34.0	39.0
12	37.12382606647628	39.0	37.0	39.0	34.0	39.0
13	36.974465858573964	39.0	37.0	39.0	33.0	39.0
14	37.99734100209562	40.0	37.0	41.0	33.0	41.0
15	38.13339417670092	40.0	37.0	41.0	33.0	41.0
16	38.1824444581238	40.0	37.0	41.0	33.0	41.0
17	38.20501583087328	40.0	37.0	41.0	33.0	41.0
18	38.15022334511839	40.0	37.0	41.0	33.0	41.0
19	38.09974922183831	40.0	37.0	41.0	34.0	41.0
20	38.06766059035909	40.0	37.0	41.0	33.0	41.0
21	38.00382055310368	40.0	37.0	41.0	33.0	41.0
22	38.05451414073018	40.0	36.0	41.0	34.0	41.0
23	38.05539065991859	40.0	36.0	41.0	34.0	41.0
24	38.01831456734745	40.0	36.0	41.0	34.0	41.0
25	37.86604244226348	40.0	36.0	41.0	33.0	41.0
26	37.793142809732444	40.0	36.0	41.0	33.0	41.0
27	37.76500721288309	40.0	36.0	41.0	33.0	41.0
28	37.72417881526722	40.0	36.0	41.0	33.0	41.0
29	37.68960782791854	40.0	36.0	41.0	33.0	41.0
30	37.598314674403234	40.0	36.0	41.0	33.0	41.0
31	37.474057708417526	40.0	36.0	41.0	33.0	41.0
32	37.40520210792828	40.0	35.0	41.0	33.0	41.0
33	37.298035258820725	40.0	35.0	41.0	32.0	41.0
34	37.16806017605323	40.0	35.0	41.0	32.0	41.0
35	37.06552750396776	39.0	35.0	41.0	31.0	41.0
36	36.91105939722244	39.0	35.0	41.0	31.0	41.0
37	36.88835420474953	39.0	35.0	41.0	31.0	41.0
38	36.799139271538955	39.0	35.0	41.0	31.0	41.0
39	36.641923845871794	39.0	35.0	41.0	30.0	41.0
40	36.54055406718285	39.0	35.0	41.0	30.0	41.0
41	36.46391818797389	39.0	35.0	41.0	30.0	41.0
42	36.43115242869416	39.0	35.0	41.0	30.0	41.0
43	36.365564705850865	39.0	35.0	41.0	30.0	41.0
44	36.230195350032254	39.0	35.0	41.0	30.0	41.0
45	36.182903459775126	39.0	35.0	40.0	30.0	41.0
46	36.05740598495331	38.0	35.0	40.0	29.0	41.0
47	35.948652033925974	38.0	35.0	40.0	29.0	41.0
48	35.827800779901345	38.0	35.0	40.0	28.0	41.0
49	35.73117089581599	38.0	35.0	40.0	28.0	41.0
50	35.55042862457412	38.0	34.0	40.0	28.0	41.0
51	33.74936368721513	36.0	32.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	32.0
9	39.0
10	45.0
11	30.0
12	25.0
13	27.0
14	19.0
15	35.0
16	51.0
17	117.0
18	196.0
19	374.0
20	647.0
21	1108.0
22	1569.0
23	2287.0
24	3339.0
25	4808.0
26	6145.0
27	7672.0
28	8652.0
29	10254.0
30	12757.0
31	16454.0
32	21967.0
33	32112.0
34	50029.0
35	58258.0
36	61452.0
37	94251.0
38	145322.0
39	207139.0
40	60.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.34410403680578	22.48211499396473	27.12860343060243	10.045177538627064
2	30.55438835019016	25.705296852292463	27.406145536978403	16.33416926053897
3	31.505578944269434	24.875882206526654	27.768529348003547	15.850009501200363
4	27.578639160468583	26.417083961170867	27.47840818762596	18.52586869073459
5	22.347090893032544	39.5042514526131	22.334779478477774	15.813878175876587
6	26.56321509914703	34.515452163463465	24.479508185752483	14.441824551637017
7	82.59661114932408	4.919480672417347	8.167017720407776	4.316890457850802
8	84.26280052564388	4.5342136886871485	7.007335997237961	4.195649788431018
9	78.08782320808699	7.0386498125185675	9.019181719155222	5.854345260239216
10	40.98978420231401	31.279022152517015	15.453769300149611	12.277424345019364
11	29.390290576147436	24.485530073306442	26.877691449187314	19.246487901358805
12	28.37861346708169	22.086543891531086	29.798306912859275	19.736535728527958
13	26.11237645094035	22.97871998758153	32.344896249568436	18.564007311909688
14	21.31708048185806	27.126596134751107	30.99666253609787	20.55966084729296
15	19.537278160353498	25.840722412394918	35.18776245393256	19.434236973319024
16	24.499848783712533	26.352315215034917	29.039549081059956	20.108286920192594
17	25.19651426384432	25.079957284744285	27.30016031602866	22.423368135382738
18	25.439530881577575	25.78732834274978	29.176179018673203	19.59696175699944
19	25.226757521337557	27.00722894145922	26.912618397000298	20.853395140202924
20	23.898730586103625	29.093746069045622	27.804794493050743	19.202728851800007
21	24.02478876556658	26.726207522274294	30.38216236614682	18.866841346012304
22	23.48295270543335	23.78123686893964	29.907503807171132	22.828306618455883
23	21.807797407644316	25.947376731961768	30.28126229468709	21.963563565706824
24	22.497905721328454	26.21849549161352	29.10405018774907	22.179548599308955
25	22.90123836772054	27.41604819651159	26.72339730808244	22.95931612768543
26	20.67942949975511	27.98103506879672	27.080160690723886	24.259374740724287
27	21.877383663823444	26.401694692977408	28.949220767750518	22.77170087544863
28	18.97242510779179	27.146401453817475	29.127334819624394	24.753838618766345
29	21.687493476288484	27.33067121296874	27.51882174409922	23.46301356664356
30	25.1907600157372	25.81342318881695	27.4999531630968	21.495863632349042
31	23.819375490114737	26.158811894967577	26.743871725765917	23.277940889151772
32	25.835637262904903	26.721390012231122	25.6054673386201	21.83750538624387
33	24.686928757055647	25.529859194887017	26.84932166782198	22.93389038023536
34	21.727773213038322	25.343314500437593	28.44699534575002	24.481916940774067
35	22.252747988020456	26.85413917786515	28.386241191316707	22.506871642797687
36	24.18952084509832	27.605938384046546	26.949017361770917	21.25552340908422
37	22.71228491824953	26.995854264968404	27.59255641170441	22.699304405077655
38	22.504730527222947	26.58783792825657	27.582386112724382	23.325045431796102
39	22.536981080567504	25.383728056910854	27.78338333730332	24.295907525218325
40	23.900737881954946	24.14950874779532	29.433246707365708	22.516506662884026
41	21.075937340252704	25.033923299887324	28.251217090384518	25.638922269475454
42	21.453175140577617	26.10086795472611	29.410229714937223	23.035727189759044
43	22.785484306961035	24.891003835273274	28.5982116332162	23.725300224549496
44	22.249402494934923	24.283328471216716	28.56087593038163	24.906393103466733
45	22.412796377232446	23.719679796165796	27.565524827573284	26.30199899902847
46	23.242612482168525	24.732293643295495	27.796899129368878	24.2281947451671
47	20.530488147587096	24.778327628152457	30.853876891207243	23.837307333053204
48	21.124781539301516	25.7011484408664	29.07113053578741	24.102939484044676
49	23.115349925194774	23.380312977569137	30.214887711870077	23.28944938536601
50	21.652031249581814	23.580641103530965	29.097894480471687	25.669433166415534
51	20.483249785219343	24.0518203496977	27.928310097768687	27.536619767314264
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	150.0
1	380.5
2	611.0
3	1882.5
4	3154.0
5	2151.5
6	1149.0
7	1083.0
8	1017.0
9	1012.5
10	1008.0
11	954.0
12	900.0
13	907.0
14	914.0
15	962.5
16	1011.0
17	1119.0
18	1227.0
19	1310.0
20	1393.0
21	1534.5
22	1676.0
23	2223.0
24	2770.0
25	3306.5
26	4924.0
27	6005.0
28	7278.5
29	8552.0
30	9908.5
31	11265.0
32	13402.0
33	15539.0
34	18920.0
35	22301.0
36	24054.0
37	25807.0
38	28518.5
39	31230.0
40	33552.5
41	35875.0
42	40945.0
43	46015.0
44	49961.5
45	53908.0
46	61989.5
47	70071.0
48	73120.5
49	76170.0
50	73467.0
51	70764.0
52	62071.0
53	53378.0
54	47906.0
55	42434.0
56	39850.5
57	37267.0
58	34368.0
59	31469.0
60	28021.5
61	24574.0
62	21776.0
63	18978.0
64	17286.0
65	15594.0
66	13174.0
67	10754.0
68	8557.5
69	6361.0
70	5577.5
71	4794.0
72	3936.5
73	3079.0
74	2489.5
75	1597.5
76	1295.0
77	929.0
78	563.0
79	404.5
80	246.0
81	182.0
82	118.0
83	85.5
84	53.0
85	51.0
86	49.0
87	35.5
88	22.0
89	16.0
90	10.0
91	6.0
92	2.0
93	2.0
94	2.0
95	3.0
96	4.0
97	3.5
98	3.0
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	747274.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.35508663585438
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.24207360713928	25.16237784162893
2	10.077031585740613	6.923945863207194
3	3.890998798900381	4.0102680250868366
4	2.162144408082123	2.971226338355576
5	1.4279797627965096	2.452918423256043
6	1.0280037418522425	2.1190294567989763
7	0.7857426911835894	1.889598076037111
8	0.6548488004704939	1.7997909818839304
9	0.5310455124816635	1.6419703130000314
>10	6.0070137783871065	44.26491812761172
>50	0.15553340559083678	3.2793692277431106
>100	0.033985448158385584	2.018252979027333
>500	0.001599315207453439	0.387771602790047
>1k	0.001999144009316799	1.0785627435733085
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTC	1752	0.23445215543428516	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGC	1650	0.2208025436453028	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1649	0.22066872392188142	No Hit
GCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCTGC	1450	0.19403859896102366	TruSeq Adapter, Index 16 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCG	1351	0.1807904463423055	No Hit
CTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCTGCT	867	0.11602170020635	TruSeq Adapter, Index 13 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCAGCGTCTATCGTATGCCGTCTTCTGC	781	0.10451320399210999	TruSeq Adapter, Index 16 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.3381972342139562E-4	0.0	0.0	0.1469340563166924	0.0
2	1.3381972342139562E-4	0.0	0.0	0.6913126911949299	0.0
3	1.3381972342139562E-4	0.0	0.0	0.9817014910193583	0.0
4	1.3381972342139562E-4	0.0	0.0	1.3889149093906652	0.0
5	1.3381972342139562E-4	0.0	0.0	2.646820309551784	0.0
6	1.3381972342139562E-4	0.0	0.0	3.479178989232865	0.0
7	1.3381972342139562E-4	0.0	0.0	4.317559556467908	0.0
8	1.3381972342139562E-4	0.0	0.0	5.628725206550743	0.0
9	1.3381972342139562E-4	0.0	0.0	6.149283930659972	0.0
10	1.3381972342139562E-4	0.0	0.0	7.359817148729917	0.0
11	1.3381972342139562E-4	0.0	0.0	8.714474208924704	0.0
12	1.3381972342139562E-4	0.0	0.0	10.081175044227418	0.0
13	1.3381972342139562E-4	0.0	0.0	10.558911456841802	0.0
14	1.3381972342139562E-4	0.0	0.0	10.78894756140318	0.0
15	1.3381972342139562E-4	0.0	0.0	11.1418301720654	0.0
16	1.3381972342139562E-4	0.0	0.0	11.768775576294638	0.0
17	1.3381972342139562E-4	0.0	0.0	12.569820440695112	0.0
18	2.6763944684279125E-4	0.0	0.0	13.428943065060473	0.0
19	4.014591702641869E-4	0.0	0.0	13.969039468789227	0.0
20	4.014591702641869E-4	0.0	0.0	14.51890471232774	0.0
21	4.014591702641869E-4	0.0	0.0	15.182382901051021	0.0
22	4.014591702641869E-4	0.0	0.0	15.924680906869503	0.0
23	4.014591702641869E-4	0.0	0.0	16.62549479842735	0.0
24	4.014591702641869E-4	0.0	0.0	17.164788283815575	0.0
25	4.014591702641869E-4	0.0	0.0	17.663802032453958	0.0
26	4.014591702641869E-4	0.0	0.0	18.12989612913068	0.0
27	4.014591702641869E-4	0.0	0.0	18.67066163147654	0.0
28	4.014591702641869E-4	0.0	0.0	19.167534264540183	0.0
29	4.014591702641869E-4	0.0	0.0	19.69183994090521	0.0
30	4.014591702641869E-4	0.0	0.0	20.291753761003328	0.0
31	4.014591702641869E-4	0.0	0.0	20.833456001413136	0.0
32	4.014591702641869E-4	0.0	0.0	21.308649839282513	0.0
33	4.014591702641869E-4	0.0	0.0	21.8177000671775	0.0
34	4.014591702641869E-4	0.0	0.0	22.350837845288343	0.0
35	4.014591702641869E-4	0.0	0.0	22.921311326233752	0.0
36	4.014591702641869E-4	0.0	0.0	23.446152281492466	0.0
37	4.014591702641869E-4	0.0	0.0	23.979423879326728	0.0
38	4.014591702641869E-4	0.0	0.0	24.575323107722202	0.0
39	4.014591702641869E-4	0.0	0.0	25.499348297946938	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGTACG	35	1.2111559E-7	45.000004	1
CGTGCTT	20	7.0317E-4	45.0	19
AACGATC	25	3.889492E-5	45.0	42
TTACGCG	25	3.889492E-5	45.0	1
ACTCACG	25	3.889492E-5	45.0	1
TAACGCG	30	2.1644792E-6	44.999996	1
CGTTTTT	730	0.0	42.22603	1
TCTACGG	60	3.6379788E-12	41.249996	2
CTACGAA	60	3.6379788E-12	41.249996	11
TACTACG	55	6.002665E-11	40.909092	1
TATAGCG	45	1.9266736E-8	40.0	1
TGCTACG	80	0.0	39.375	1
TCGACTG	40	3.4560617E-7	39.375	1
AAGTGCG	40	3.4560617E-7	39.375	1
TACGGGT	75	0.0	39.0	4
CGACAGG	75	0.0	39.0	2
GCGATCA	75	0.0	39.0	9
TAACGCC	35	6.2453546E-6	38.57143	12
GGAACGC	70	0.0	38.57143	8
CCACGTC	35	6.2453546E-6	38.57143	33
>>END_MODULE