##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936748.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	357047
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.94344722123418	33.0	31.0	34.0	30.0	34.0
2	32.046360843250326	33.0	31.0	34.0	30.0	34.0
3	32.00396026293457	33.0	31.0	34.0	30.0	34.0
4	35.69087263021395	37.0	35.0	37.0	33.0	37.0
5	30.77688091483754	37.0	35.0	37.0	0.0	37.0
6	33.190451117079824	37.0	35.0	37.0	17.0	37.0
7	35.215103333734774	37.0	35.0	37.0	32.0	37.0
8	35.68435248020569	37.0	35.0	37.0	35.0	37.0
9	37.66121266948049	39.0	37.0	39.0	35.0	39.0
10	37.31798614748198	39.0	37.0	39.0	34.0	39.0
11	37.14847625102578	39.0	37.0	39.0	34.0	39.0
12	36.97773122306027	39.0	35.0	39.0	33.0	39.0
13	36.75500144238713	39.0	35.0	39.0	33.0	39.0
14	37.896806302811676	40.0	37.0	41.0	33.0	41.0
15	38.05745462082023	40.0	37.0	41.0	33.0	41.0
16	38.044246275700395	40.0	36.0	41.0	33.0	41.0
17	38.049116782944544	40.0	36.0	41.0	34.0	41.0
18	38.01358644660227	40.0	36.0	41.0	34.0	41.0
19	37.99438449279787	40.0	36.0	41.0	34.0	41.0
20	37.99067349676653	40.0	36.0	41.0	34.0	41.0
21	37.87822891664123	40.0	36.0	41.0	33.0	41.0
22	37.85989239511885	40.0	36.0	41.0	33.0	41.0
23	37.87432746949281	40.0	36.0	41.0	34.0	41.0
24	37.82839514125591	40.0	36.0	41.0	33.0	41.0
25	37.65535349687856	39.0	35.0	41.0	33.0	41.0
26	37.63588267090887	39.0	35.0	41.0	33.0	41.0
27	37.55774729937515	39.0	35.0	41.0	33.0	41.0
28	37.53382327816786	39.0	36.0	41.0	33.0	41.0
29	37.5567502317622	40.0	36.0	41.0	33.0	41.0
30	37.486515780835575	39.0	36.0	41.0	33.0	41.0
31	37.415844412640354	39.0	35.0	41.0	33.0	41.0
32	37.35396180334802	39.0	35.0	41.0	33.0	41.0
33	37.264418970051565	39.0	35.0	41.0	32.0	41.0
34	37.20909852204332	39.0	35.0	41.0	32.0	41.0
35	37.17154044145449	39.0	35.0	41.0	32.0	41.0
36	37.02832400216218	39.0	35.0	41.0	32.0	41.0
37	36.950771186986586	39.0	35.0	41.0	31.0	41.0
38	36.915439704016556	39.0	35.0	41.0	31.0	41.0
39	36.83013720882685	39.0	35.0	41.0	31.0	41.0
40	36.740838040930186	39.0	35.0	41.0	31.0	41.0
41	36.706414001518006	39.0	35.0	41.0	31.0	41.0
42	36.654376034527665	39.0	35.0	41.0	31.0	41.0
43	36.55818981814719	39.0	35.0	41.0	31.0	41.0
44	36.42734149845818	39.0	35.0	40.0	30.0	41.0
45	36.35401221687901	39.0	35.0	40.0	30.0	41.0
46	36.14894677731503	38.0	35.0	40.0	30.0	41.0
47	36.084817965141845	38.0	35.0	40.0	30.0	41.0
48	36.00104468039222	38.0	35.0	40.0	29.0	41.0
49	35.90708226087882	38.0	35.0	40.0	29.0	41.0
50	35.69985464098564	38.0	35.0	40.0	28.0	41.0
51	33.81222640156618	36.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	17.0
9	27.0
10	23.0
11	19.0
12	11.0
13	6.0
14	2.0
15	11.0
16	18.0
17	23.0
18	72.0
19	139.0
20	254.0
21	402.0
22	639.0
23	975.0
24	1358.0
25	1804.0
26	2397.0
27	3033.0
28	3775.0
29	4978.0
30	6127.0
31	8250.0
32	10820.0
33	16518.0
34	25802.0
35	34071.0
36	29779.0
37	44539.0
38	69032.0
39	92102.0
40	23.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.18126465143245	22.70681450901422	26.180866944687953	10.931053894865382
2	30.943825322716616	24.77124860312508	26.59453797399222	17.690388100166086
3	30.9687520130403	24.65949860942677	28.176962696787815	16.194786680745114
4	28.67353597705624	25.340641428159316	27.866359330844396	18.11946326394004
5	21.02160219802995	41.102992043064354	21.67977885264405	16.19562690626164
6	25.21180684895826	34.280640924024006	25.264460981327385	15.243091245690344
7	80.04884511002753	5.757225239254216	9.144174296381149	5.049755354337105
8	81.15206121322963	4.833257246244893	8.306189381229922	5.708492159295555
9	75.6222570137825	5.957198912188031	11.194044481538846	7.226499592490625
10	41.523945026845205	23.61677874341473	18.850459463319954	16.008816766420107
11	30.95838923166978	25.576464723131686	24.25871103804261	19.20643500715592
12	28.663453270857897	22.313869042450992	29.49779720877083	19.524880477920274
13	22.77123179861475	27.441205219480906	31.33705086445202	18.450512117452327
14	18.104059129470347	31.11607155360499	28.3349250938952	22.444944223029463
15	17.19381481989766	25.983694023475902	37.85019899340983	18.972292163216608
16	19.364957554607656	25.095855727677307	34.132201082770614	21.40698563494442
17	18.908435023960433	24.83174484031514	28.87350964999006	27.386310485734374
18	20.422521404745034	26.434615050679604	30.798466308357163	22.3443972362182
19	22.17579198256812	27.130881928709666	28.123468338902164	22.569857749820052
20	24.72671665074906	25.52885194386173	28.540780345444716	21.20365105994449
21	22.267936714214095	28.417547269687184	28.789487098337197	20.525028917761524
22	20.052822177472436	26.43433497550743	28.06157172585122	25.451271121168922
23	19.685923701921595	26.87629359720149	28.69370138945293	24.744081311423987
24	21.732993135357532	23.77502121569429	31.207095984562255	23.284889664385922
25	18.860822244690475	26.127652661974476	30.452293395547365	24.559231697787688
26	17.92425086893322	28.115066083736874	29.07152279671864	24.889160250611265
27	20.0214537581887	28.45087621517615	29.26533481586458	22.26233521077057
28	17.28175842396099	27.31993266992861	32.02827638938291	23.37003251672749
29	18.31691626032427	24.751643341072743	31.868353466070293	25.06308693253269
30	19.86069060935955	27.242911997580148	30.06971071035466	22.82668668270564
31	20.926656714662215	28.41334614210454	25.813968469137116	24.846028674096125
32	22.36512279895924	27.878962713592326	27.244872523785386	22.511041963663047
33	21.133632266900438	27.17513380591351	27.658823628261825	24.03241029892423
34	18.768397437872324	25.721263587146794	29.076564149817813	26.433774825163077
35	19.45486168487622	24.157883976059175	29.61430848039614	26.772945858668468
36	21.057451820068508	25.790442154674313	28.257624346374566	24.894481678882613
37	20.24439359524096	26.302699644584603	31.08022193156643	22.372684828607998
38	19.783109786666742	28.701263419101686	27.41711875467375	24.098508039557817
39	20.40207591717617	25.76859629124457	29.33031225580946	24.4990155357698
40	22.005506277884983	23.82179376944772	29.119135575988597	25.0535643766787
41	18.960529005985208	23.865765571479386	27.54875408559657	29.62495133693883
42	20.972589042899116	23.087436668001693	29.16814873111943	26.77182555797976
43	21.470002548684068	23.9562298520923	28.93344573683577	25.640321862387864
44	19.710570317073103	25.815648920170176	28.202169462283678	26.271611300473047
45	19.13641621411187	26.071917702711406	27.40143454503189	27.39023153814484
46	22.562015644999118	25.961288009701804	27.361663870582863	24.115032474716212
47	19.06387674451823	24.299041862835985	30.70100014843984	25.936081244205944
48	19.83576391903587	22.830607735116104	30.64358473814372	26.690043607704304
49	20.323094718622478	21.777805162905725	31.409030183701304	26.490069934770492
50	18.761115483395745	22.993331410150482	31.217458765932776	27.028094340520997
51	18.75831473167398	23.205628390660053	27.687111220651623	30.348945657014344
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	87.0
1	206.5
2	326.0
3	455.0
4	584.0
5	441.0
6	298.0
7	309.0
8	320.0
9	304.0
10	288.0
11	297.5
12	307.0
13	334.5
14	362.0
15	340.5
16	319.0
17	380.0
18	441.0
19	495.5
20	550.0
21	686.5
22	823.0
23	1108.0
24	1393.0
25	1813.5
26	2804.5
27	3375.0
28	3979.0
29	4583.0
30	5220.0
31	5857.0
32	6379.5
33	6902.0
34	8315.0
35	9728.0
36	10407.5
37	11087.0
38	12007.5
39	12928.0
40	15781.5
41	18635.0
42	21919.0
43	25203.0
44	28266.5
45	31330.0
46	35092.5
47	38855.0
48	40384.5
49	41914.0
50	40216.0
51	38518.0
52	32480.0
53	26442.0
54	22380.0
55	18318.0
56	15575.0
57	12832.0
58	12177.5
59	11523.0
60	10591.5
61	9660.0
62	8996.5
63	8333.0
64	6676.0
65	5019.0
66	4078.0
67	3137.0
68	2470.5
69	1804.0
70	1426.0
71	1048.0
72	861.0
73	674.0
74	603.5
75	372.5
76	212.0
77	171.0
78	130.0
79	101.5
80	73.0
81	51.5
82	30.0
83	22.5
84	15.0
85	11.0
86	7.0
87	5.5
88	4.0
89	3.5
90	3.0
91	2.0
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	357047.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	29.17341979361335
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.15218079412224	19.590587603635477
2	13.137084772513754	7.665073778656585
3	5.398121873972304	4.724450265794424
4	2.91910985959852	3.406416694309734
5	1.7635887950178286	2.5724958130183917
6	1.2304089550439725	2.153714217799137
7	0.8408493871357259	1.7171316507879126
8	0.6236615909973741	1.4555473122655347
9	0.5080946969018902	1.3340573898864823
>10	5.802241726807333	42.06000690012638
>50	0.5848069866202266	10.28183234030931
>100	0.03387297707851398	1.6569630871806698
>500	0.004981320158604997	1.0140579216231111
>1k	9.962640317209995E-4	0.36766502460683165
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGC	1265	0.3542950928029083	TruSeq Adapter, Index 13 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGC	793	0.2220996115357362	TruSeq Adapter, Index 13 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGCT	736	0.2061353267216921	Illumina Single End Adapter 2 (95% over 22bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGC	728	0.2038947253442824	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTC	656	0.18372931294759512	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCG	576	0.16132329917349816	No Hit
TCTGTCTCTTATACACATCTGACGCCTATACGCTCGTATGCCGTCTTCTGC	473	0.13247555643934833	TruSeq Adapter, Index 13 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.26355073701781556	0.0
2	2.800751721762121E-4	0.0	0.0	1.3183138354334303	0.0
3	2.800751721762121E-4	0.0	0.0	1.7832386212459423	0.0
4	2.800751721762121E-4	0.0	0.0	2.3868006172856795	0.0
5	2.800751721762121E-4	0.0	0.0	4.184603147484785	0.0
6	2.800751721762121E-4	0.0	0.0	5.249729027270919	0.0
7	2.800751721762121E-4	0.0	0.0	6.378151895968878	0.0
8	2.800751721762121E-4	0.0	0.0	8.161390517214821	0.0
9	2.800751721762121E-4	0.0	0.0	8.766352889115439	0.0
10	2.800751721762121E-4	0.0	0.0	10.292202427131443	0.0
11	2.800751721762121E-4	0.0	0.0	12.109330144210706	0.0
12	2.800751721762121E-4	0.0	0.0	13.77045599038782	0.0
13	2.800751721762121E-4	0.0	0.0	14.382980391937195	0.0
14	2.800751721762121E-4	0.0	0.0	14.644850677921955	0.0
15	2.800751721762121E-4	0.0	0.0	15.134702154058148	0.0
16	2.800751721762121E-4	0.0	0.0	16.12616826356194	0.0
17	2.800751721762121E-4	0.0	0.0	17.294081731536746	0.0
18	2.800751721762121E-4	0.0	0.0	18.555540307018404	0.0
19	2.800751721762121E-4	0.0	0.0	19.254047786425875	0.0
20	2.800751721762121E-4	0.0	0.0	20.004369172685948	0.0
21	2.800751721762121E-4	0.0	0.0	20.896968746411538	0.0
22	2.800751721762121E-4	0.0	0.0	21.7839668166936	0.0
23	2.800751721762121E-4	0.0	0.0	22.665643458704317	0.0
24	5.601503443524242E-4	0.0	0.0	23.361350186390027	0.0
25	5.601503443524242E-4	0.0	0.0	24.031289998235525	0.0
26	5.601503443524242E-4	0.0	0.0	24.586118914316604	0.0
27	5.601503443524242E-4	0.0	0.0	25.13198542488804	0.0
28	5.601503443524242E-4	0.0	0.0	25.67393088304901	0.0
29	5.601503443524242E-4	0.0	0.0	26.24024288118931	0.0
30	5.601503443524242E-4	0.0	0.0	26.907101866140874	0.0
31	5.601503443524242E-4	0.0	0.0	27.527188297339006	0.0
32	5.601503443524242E-4	0.0	0.0	28.12430856441869	0.0
33	5.601503443524242E-4	0.0	0.0	28.678577330155413	0.0
34	5.601503443524242E-4	0.0	0.0	29.257492711043643	0.0
35	5.601503443524242E-4	0.0	0.0	29.845930647785867	0.0
36	5.601503443524242E-4	0.0	0.0	30.405240766621763	0.0
37	8.402255165286363E-4	0.0	0.0	30.981075320616053	0.0
38	8.402255165286363E-4	0.0	0.0	31.490251983632408	0.0
39	8.402255165286363E-4	0.0	0.0	32.036958719720374	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTCGCT	40	6.7921064E-9	45.0	20
CGGGTAT	20	7.026747E-4	45.0	6
CGGGTAC	35	1.2087912E-7	45.0	6
GGGCTTA	40	6.7921064E-9	45.0	7
GTACAAG	40	6.7921064E-9	45.0	1
ACGTTGA	20	7.026747E-4	45.0	32
TAGACGG	20	7.026747E-4	45.0	2
GCGCGAC	35	1.2087912E-7	45.0	9
GTTTACG	25	3.8853846E-5	45.0	1
TAGCGTG	25	3.8853846E-5	45.0	1
CGTTGCA	20	7.026747E-4	45.0	16
CGACCTC	35	1.2087912E-7	45.0	12
TAAACGG	20	7.026747E-4	45.0	2
ACCGAAT	25	3.8853846E-5	45.0	34
TCGGGCA	35	1.2087912E-7	45.0	5
CCAGCGG	25	3.8853846E-5	45.0	2
TCCTCCG	20	7.026747E-4	45.0	34
CGCGACC	35	1.2087912E-7	45.0	10
CGTTATT	35	1.2087912E-7	45.0	1
GTTACCG	20	7.026747E-4	45.0	21
>>END_MODULE