##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936747.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	326237
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.925361010553676	31.0	30.0	33.0	28.0	34.0
2	31.005989510693148	31.0	31.0	34.0	28.0	34.0
3	30.6252540331109	31.0	30.0	34.0	27.0	34.0
4	34.60751232999323	35.0	35.0	37.0	32.0	37.0
5	34.96610746175327	35.0	35.0	37.0	32.0	37.0
6	34.99330548037163	35.0	35.0	37.0	32.0	37.0
7	31.121629367606985	35.0	35.0	37.0	0.0	37.0
8	33.093361574560824	35.0	35.0	37.0	17.0	37.0
9	36.21362996839721	39.0	35.0	39.0	32.0	39.0
10	35.98392886153318	37.0	35.0	39.0	32.0	39.0
11	36.27432510720734	37.0	35.0	39.0	32.0	39.0
12	36.333460030591255	38.0	35.0	39.0	32.0	39.0
13	36.22273684468653	38.0	35.0	39.0	32.0	39.0
14	37.27355572789107	39.0	36.0	40.0	32.0	41.0
15	37.459092622847805	39.0	36.0	41.0	32.0	41.0
16	37.44799639525866	39.0	36.0	40.0	32.0	41.0
17	37.312904422245175	39.0	36.0	40.0	32.0	41.0
18	37.17093401422892	39.0	36.0	40.0	32.0	41.0
19	36.84644292339618	38.0	35.0	40.0	31.0	41.0
20	36.71452962110368	38.0	35.0	40.0	31.0	41.0
21	36.76621290656792	38.0	35.0	40.0	31.0	41.0
22	36.84199217133556	38.0	35.0	40.0	32.0	41.0
23	36.89085848631516	38.0	35.0	40.0	32.0	41.0
24	36.810193203100816	38.0	35.0	40.0	32.0	41.0
25	36.54529682408801	38.0	35.0	40.0	31.0	41.0
26	36.50661635559425	38.0	35.0	40.0	31.0	41.0
27	36.50245680287644	38.0	35.0	40.0	31.0	41.0
28	36.52664167461079	38.0	35.0	40.0	31.0	41.0
29	36.45003479065833	38.0	35.0	40.0	31.0	41.0
30	36.149725506303696	38.0	35.0	40.0	30.0	41.0
31	36.06142160453903	38.0	35.0	40.0	30.0	41.0
32	35.73952678574165	38.0	35.0	40.0	29.0	41.0
33	35.43440504909008	38.0	34.0	40.0	27.0	41.0
34	35.20122794164978	38.0	34.0	40.0	25.0	41.0
35	34.94078537995384	38.0	34.0	40.0	24.0	41.0
36	34.83586165885537	38.0	34.0	40.0	23.0	41.0
37	34.587174354840194	38.0	34.0	40.0	23.0	41.0
38	34.54803409791042	38.0	34.0	40.0	22.0	41.0
39	34.53174839150679	38.0	34.0	40.0	22.0	41.0
40	34.41282871041605	38.0	34.0	40.0	22.0	41.0
41	34.240555179210205	38.0	33.0	40.0	21.0	41.0
42	34.22185098563315	38.0	33.0	40.0	21.0	41.0
43	34.10962275891453	38.0	33.0	40.0	20.0	41.0
44	33.88849210849781	37.0	33.0	40.0	18.0	41.0
45	33.83229063533566	37.0	33.0	40.0	20.0	41.0
46	33.68556294963477	37.0	33.0	40.0	19.0	41.0
47	33.5945677528913	37.0	32.0	40.0	19.0	41.0
48	33.504495198276096	37.0	32.0	40.0	18.0	41.0
49	33.38755567271646	37.0	32.0	40.0	18.0	41.0
50	33.18795844738641	36.0	32.0	40.0	18.0	41.0
51	31.368744195170994	35.0	29.0	39.0	12.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	6.0
9	17.0
10	25.0
11	14.0
12	11.0
13	9.0
14	8.0
15	18.0
16	53.0
17	99.0
18	212.0
19	411.0
20	720.0
21	1054.0
22	1748.0
23	2527.0
24	3868.0
25	5445.0
26	6857.0
27	7388.0
28	7368.0
29	7719.0
30	8416.0
31	10547.0
32	13118.0
33	17758.0
34	26753.0
35	29954.0
36	31419.0
37	44687.0
38	57007.0
39	40999.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.16642502229974	20.531392821782937	25.14399041187848	16.158191744038845
2	36.161440915653344	24.70535224392083	24.09322057277378	15.039986267652045
3	27.815667750745625	24.31821038079678	33.17833354279251	14.687788325665082
4	24.93310078255992	22.954171353954333	35.05089857986678	17.061829283618966
5	25.22675233036106	26.205488647823515	31.079246069575184	17.488512952240242
6	22.79201929885329	31.802953067861704	31.700880035066533	13.704147598218471
7	63.34965071405144	16.387472910797978	16.703500829151814	3.5593755459987677
8	73.21180614093436	5.9318838758326	15.965080600912835	4.891229382320215
9	68.23137780202735	5.768199192611506	17.213865993127694	8.786557012233438
10	37.4197286022125	23.579177101309785	25.34997563121289	13.651118665264821
11	27.109126187403636	23.257325196099767	31.984109711651342	17.649438904845248
12	24.541361035075727	20.68312300566766	36.32451254762642	18.451003411630197
13	20.224560672149387	25.971609596704237	37.63215085965111	16.171678871495264
14	17.826917241146774	28.200663934501602	35.12875608836521	18.843662735986417
15	15.476785281865638	24.729567768217585	42.671738643991944	17.121908305924833
16	18.535604483856826	24.27897510092356	36.81372744354564	20.371692971673966
17	17.797797306865867	23.796503768732546	34.912348997814476	23.49334992658711
18	19.55756091430463	24.400052722407327	36.555939393753626	19.48644696953442
19	19.655036062739665	27.04506233198564	32.87058181628693	20.42931978898776
20	20.944282837323787	25.338327657500532	35.41566407243813	18.301725432737552
21	18.930102961957104	27.342392187274893	35.433136033006676	18.29436881776132
22	18.29590144588137	23.259470875467837	34.1132366960216	24.33139098262919
23	16.328619990988148	26.411167341533915	34.8393958993002	22.420816768177737
24	19.25747232839929	23.624542893663193	35.34638928141198	21.77159549652553
25	16.728022879072576	27.85153124875474	33.208679579569456	22.21176629260323
26	16.364483488997266	27.057936408194045	34.764603647041874	21.81297645576682
27	18.63491878603592	26.970576605351326	34.99081955756091	19.40368505105184
28	15.290417702467836	24.911643988879252	38.70253833869242	21.09539996996049
29	18.696530436461835	23.589905498150117	36.483599346487374	21.229964718900675
30	17.803008242474032	25.767770056737895	38.336546743625036	18.092674957163045
31	20.54794520547945	26.040271336482373	33.46616110373747	19.945622354300706
32	20.610476432777396	27.99191998455111	31.517577711908828	19.880025870762665
33	19.985470685421948	25.235641573457336	33.91276893791936	20.866118803201353
34	21.60699123643241	24.61983159482217	33.2102122076895	20.56296496105592
35	18.925505077596963	25.100157247645114	31.658272973329204	24.316064701428715
36	23.655808507312166	23.652436725448066	34.03660529001922	18.655149477220547
37	19.24367867531886	26.243497825200695	36.003273693664426	18.509549805816018
38	18.157351863829057	27.00061611650426	32.419069572120875	22.422962447545803
39	21.93803891036271	26.299285488770437	31.978285724795164	19.78438987607169
40	21.376177441553228	24.46043827033721	32.98644850216254	21.176935785947027
41	21.478863525596424	24.57293317434871	30.372091454985178	23.57611184506969
42	21.482848358708544	23.56293124323728	32.22779758273893	22.726422815315246
43	22.62434978252007	23.912063928984143	30.656547234066032	22.807039054429758
44	19.454568304637426	25.611441988493027	31.85291674457526	23.081072962294282
45	19.43249845970874	25.291429237027067	30.35400644316862	24.922065860095575
46	22.720292302835055	26.16870557294237	30.765976881837435	20.345025242385137
47	19.02727158476813	24.016589166771396	35.30960620653084	21.64653304192964
48	19.880945447634694	24.130310173278936	32.54535812921281	23.44338624987356
49	20.236821697109768	22.210540190107192	35.05059205424278	22.502046058540266
50	19.168579897436526	22.82481754062231	34.29163460919516	23.71496795274601
51	18.790327277408757	23.172111072625114	30.888280605817243	27.149281044148886
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	111.0
1	233.5
2	356.0
3	4303.0
4	8250.0
5	5677.5
6	3105.0
7	2936.5
8	2768.0
9	2777.0
10	2786.0
11	2749.0
12	2712.0
13	2556.0
14	2400.0
15	2378.0
16	2356.0
17	2235.0
18	2114.0
19	1885.0
20	1656.0
21	1761.5
22	1867.0
23	1901.0
24	1935.0
25	2163.0
26	2868.0
27	3345.0
28	3648.5
29	3952.0
30	4690.5
31	5429.0
32	6550.0
33	7671.0
34	8759.0
35	9847.0
36	10440.0
37	11033.0
38	12237.5
39	13442.0
40	14944.5
41	16447.0
42	19041.5
43	21636.0
44	24294.5
45	26953.0
46	33782.5
47	40612.0
48	37249.5
49	33887.0
50	31195.0
51	28503.0
52	24224.5
53	19946.0
54	16376.5
55	12807.0
56	11053.5
57	9300.0
58	8108.5
59	6917.0
60	6461.0
61	6005.0
62	5083.0
63	4161.0
64	3675.5
65	3190.0
66	2729.0
67	2268.0
68	1881.5
69	1495.0
70	1246.5
71	998.0
72	801.5
73	605.0
74	515.5
75	308.5
76	191.0
77	224.0
78	257.0
79	161.5
80	66.0
81	46.5
82	27.0
83	17.0
84	7.0
85	4.5
86	2.0
87	2.5
88	3.0
89	2.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	326237.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.934409131528845
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.46454936203179	33.3229892440001
2	9.096237815989637	7.628907162667876
3	3.058342541935501	3.8474936225364957
4	1.649158897754618	2.766260157653732
5	1.0742385159486116	2.2523778716317726
6	0.7519457088170612	1.891943939893926
7	0.5766641889638231	1.6927450422057153
8	0.41283973308230365	1.3849752218259588
9	0.3002881075506238	1.1333163923424356
>10	3.4097064318435772	32.76038256014834
>50	0.17607416934703662	4.446990215343068
>100	0.024840339243980266	2.3348613455824676
>500	0.0014611964261164864	0.3627444998254353
>1k	0.0036529910652912155	4.174012724342678
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4880	1.4958450451665506	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGC	2589	0.7935948405606966	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCG	2556	0.7834794949683819	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTCTTC	2353	0.7212547932944455	No Hit
GCTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTCTTCTGC	1246	0.3819309275158857	No Hit
CCTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTCTTCTGC	594	0.18207622066166623	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTCT	590	0.18085011816562807	No Hit
TCTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTCTTCTGC	496	0.15203670950873138	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	457	0.14008221017235936	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTC	443	0.13579085143622582	No Hit
GAACTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTCTTCT	440	0.1348712745641972	No Hit
CTGTCTCTTATACACATCTGACGCGTTATGCGTCGTATGCCGTCTTCTGCT	425	0.13027339020405412	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTTATGCGTCGTA	408	0.12506245459589194	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	328	0.10054040467512883	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1606194269809985	0.0
2	0.0	0.0	0.0	1.1359839625793517	0.0
3	0.0	0.0	0.0	1.5310954919276476	0.0
4	0.0	0.0	0.0	2.1941104166602807	0.0
5	0.0	0.0	0.0	4.245073366908107	0.0
6	0.0	0.0	0.0	5.101505960390758	0.0
7	0.0	0.0	0.0	5.9683604250897355	0.0
8	0.0	0.0	0.0	7.398302461094235	0.0
9	0.0	0.0	0.0	7.848588602764248	0.0
10	0.0	0.0	0.0	9.811885224545346	0.0
11	0.0	0.0	0.0	11.531187449614851	0.0
12	3.0652562400953907E-4	0.0	0.0	13.669816728329405	0.0
13	3.0652562400953907E-4	0.0	0.0	14.222788954042613	0.0
14	3.0652562400953907E-4	0.0	0.0	14.471994286362369	0.0
15	3.0652562400953907E-4	0.0	0.0	14.978068091602118	0.0
16	3.0652562400953907E-4	0.0	0.0	15.86729892685379	0.0
17	3.0652562400953907E-4	0.0	0.0	16.989795761976723	0.0
18	3.0652562400953907E-4	0.0	0.0	18.16930636316543	0.0
19	3.0652562400953907E-4	0.0	0.0	18.948494499397675	0.0
20	3.0652562400953907E-4	0.0	0.0	19.62438350033871	0.0
21	3.0652562400953907E-4	0.0	0.0	20.431158942731816	0.0
22	3.0652562400953907E-4	0.0	0.0	21.259391178805593	0.0
23	3.0652562400953907E-4	0.0	0.0	22.150767693425333	0.0
24	3.0652562400953907E-4	0.0	0.0	22.787727940117154	0.0
25	3.0652562400953907E-4	0.0	0.0	23.357252549526876	0.0
26	3.0652562400953907E-4	0.0	0.0	23.924938005192544	0.0
27	3.0652562400953907E-4	0.0	0.0	24.45982521908919	0.0
28	3.0652562400953907E-4	0.0	0.0	25.0063604066982	0.0
29	3.0652562400953907E-4	0.0	0.0	25.593663502300476	0.0
30	3.0652562400953907E-4	0.0	0.0	26.20855390406361	0.0
31	3.0652562400953907E-4	0.0	0.0	26.84612720200345	0.0
32	3.0652562400953907E-4	0.0	0.0	27.38070789027609	0.0
33	3.0652562400953907E-4	0.0	0.0	27.91620815542075	0.0
34	3.0652562400953907E-4	0.0	0.0	28.445271382461218	0.0
35	3.0652562400953907E-4	0.0	0.0	29.005906748774663	0.0
36	3.0652562400953907E-4	0.0	0.0	29.538648283303242	0.0
37	3.0652562400953907E-4	0.0	0.0	30.07230939470385	0.0
38	3.0652562400953907E-4	0.0	0.0	30.609955339216583	0.0
39	3.0652562400953907E-4	0.0	0.0	31.145149078737237	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CATGCTA	25	3.8846425E-5	45.000004	40
ACTTGCG	30	2.1607048E-6	45.000004	1
GTCTACG	25	3.8846425E-5	45.000004	1
TCGATCA	25	3.8846425E-5	45.000004	17
ACGGCTC	25	3.8846425E-5	45.000004	29
AGTTCGG	25	3.8846425E-5	45.000004	2
CTCGATC	25	3.8846425E-5	45.000004	16
TGATCGG	35	1.2083547E-7	45.0	2
ACCGCGT	20	7.025851E-4	45.0	44
TAGCGTG	20	7.025851E-4	45.0	1
CCTACCT	20	7.025851E-4	45.0	19
GCGTTTG	20	7.025851E-4	45.0	1
TACGCGG	20	7.025851E-4	45.0	2
TGACCGG	40	6.7884685E-9	45.0	2
AGTTGCG	20	7.025851E-4	45.0	1
CTACGAA	45	3.8380676E-10	45.0	11
TACGAAT	45	3.8380676E-10	45.0	12
ACGGAGC	35	1.2083547E-7	45.0	21
AGCGCCC	20	7.025851E-4	45.0	32
TCGTCAT	20	7.025851E-4	45.0	2
>>END_MODULE