##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936746.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	462004
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.073871654790867	31.0	31.0	33.0	28.0	34.0
2	31.139293166292934	31.0	31.0	34.0	28.0	34.0
3	30.8974208015515	31.0	31.0	34.0	27.0	34.0
4	34.87350975316231	35.0	35.0	37.0	32.0	37.0
5	35.130317053532	35.0	35.0	37.0	33.0	37.0
6	35.102074873810615	36.0	35.0	37.0	32.0	37.0
7	31.018839663725856	35.0	35.0	37.0	0.0	37.0
8	33.07763569146587	35.0	35.0	37.0	17.0	37.0
9	36.35610514194682	39.0	35.0	39.0	32.0	39.0
10	36.075302378334385	37.0	35.0	39.0	32.0	39.0
11	36.322795906528945	37.0	35.0	39.0	32.0	39.0
12	36.35042553744123	38.0	35.0	39.0	32.0	39.0
13	36.25973584644289	38.0	35.0	39.0	32.0	39.0
14	37.19851559726756	39.0	36.0	40.0	32.0	41.0
15	37.448351096527304	39.0	36.0	41.0	32.0	41.0
16	37.37708980874624	39.0	36.0	40.0	32.0	41.0
17	37.323780746487046	39.0	36.0	40.0	32.0	41.0
18	37.263224993723	39.0	36.0	40.0	32.0	41.0
19	37.02957333702739	39.0	35.0	40.0	32.0	41.0
20	37.039454203859705	39.0	35.0	40.0	32.0	41.0
21	36.989634288880616	39.0	35.0	40.0	31.0	41.0
22	37.07018337503571	39.0	35.0	40.0	32.0	41.0
23	37.12999021653492	39.0	35.0	40.0	32.0	41.0
24	37.09865066103324	39.0	35.0	40.0	32.0	41.0
25	36.77424437883655	39.0	35.0	40.0	31.0	41.0
26	36.785770253071405	39.0	35.0	40.0	31.0	41.0
27	36.78784382819197	39.0	35.0	40.0	31.0	41.0
28	36.787114397277946	39.0	35.0	40.0	31.0	41.0
29	36.771668643561526	39.0	35.0	40.0	31.0	41.0
30	36.534800564497274	38.0	35.0	40.0	31.0	41.0
31	36.48443952866209	38.0	35.0	40.0	30.0	41.0
32	36.33381096267565	38.0	35.0	40.0	30.0	41.0
33	36.17853525077705	38.0	35.0	40.0	30.0	41.0
34	36.10804019012822	38.0	35.0	40.0	30.0	41.0
35	36.03673994164553	38.0	35.0	40.0	30.0	41.0
36	35.90867827984173	38.0	35.0	40.0	29.0	41.0
37	35.80680253850616	38.0	35.0	40.0	29.0	41.0
38	35.660829776365574	38.0	35.0	40.0	28.0	41.0
39	35.60776097176648	38.0	35.0	40.0	28.0	41.0
40	35.504958831525265	38.0	34.0	40.0	27.0	41.0
41	35.35516575614064	38.0	34.0	40.0	27.0	41.0
42	35.33678712738418	38.0	34.0	40.0	27.0	41.0
43	35.27749110397313	38.0	34.0	40.0	27.0	41.0
44	35.07353183089324	38.0	34.0	40.0	26.0	41.0
45	34.982365953541525	38.0	34.0	40.0	26.0	41.0
46	34.89383858148414	37.0	34.0	40.0	26.0	41.0
47	34.86586912667423	37.0	34.0	40.0	26.0	41.0
48	34.77128552999541	37.0	34.0	40.0	26.0	41.0
49	34.66176916217176	37.0	34.0	40.0	26.0	41.0
50	34.49290698781829	37.0	33.0	40.0	24.0	41.0
51	32.80130907957507	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	13.0
9	30.0
10	42.0
11	27.0
12	20.0
13	16.0
14	31.0
15	42.0
16	68.0
17	132.0
18	213.0
19	467.0
20	753.0
21	1192.0
22	1728.0
23	2538.0
24	3466.0
25	4736.0
26	5980.0
27	7040.0
28	7888.0
29	9111.0
30	10856.0
31	13947.0
32	17979.0
33	24897.0
34	37762.0
35	41686.0
36	48185.0
37	69110.0
38	87301.0
39	64743.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.75569908485641	21.123843083609668	24.39978874641778	12.720669085116146
2	34.931082847767556	22.29309702946295	25.01861455744972	17.75720556531978
3	33.08088241660245	22.097644176240898	28.160578696288347	16.660894710868305
4	29.161219383381965	25.75735275019264	26.02812962658332	19.05329823984208
5	25.37878459926754	29.91748989186241	26.22812789499658	18.475597613873475
6	26.001073583778496	33.89754201262327	26.166873014086455	13.934511389511778
7	69.63229755586532	17.660020259564853	9.088233002311668	3.6194491822581623
8	80.61099038103566	5.145842893135125	9.879568142267168	4.363598583562047
9	74.46515614583423	7.5995013030190215	12.111150552809066	5.824191998337676
10	42.393788798365385	24.16169556973533	18.784902295218224	14.659613336681069
11	30.867914563510272	26.250422074267753	24.73788105730686	18.14378230491511
12	28.635466359598617	23.307806858815074	29.64000311685613	18.416723664730174
13	24.634418749621215	25.477917940104415	31.140206578298024	18.747456731976346
14	20.398091791413062	27.74543077549112	31.08674383771569	20.769733595380128
15	20.02580064241868	25.381381979376798	34.62654002995645	19.966277348248067
16	22.88681483277201	25.029869871256526	32.29495848520792	19.788356810763545
17	23.330966831456003	24.25476835698392	28.979186327391105	23.435078484168965
18	25.894797447641142	23.557371797646773	30.870511943619533	19.677318811092544
19	24.95714322819716	25.843066293798323	27.936987558549276	21.262802919455243
20	26.65712850970987	25.648695682288462	28.326378126596307	19.367797681405357
21	25.119046588341227	26.088735162466126	29.43762391667605	19.3545943325166
22	24.208015515017188	23.27663829750392	29.51208214647492	23.003264041003973
23	21.614531475917957	25.814711560938868	29.889351607345393	22.681405355797786
24	22.667336213539276	24.840477571622756	31.336092328204952	21.156093886633016
25	22.51798685725665	26.01774010614627	29.321607605128957	22.142665431468124
26	22.230759906840632	26.719249183989753	28.422264742296605	22.627726166873014
27	21.79569873853906	26.325746097436387	29.200829430048223	22.677725733976327
28	20.33640401381806	27.38829101046744	30.738045558047116	21.53725941766738
29	21.222327079419227	25.961679985454673	30.145193548107805	22.670799387018295
30	22.583787153357978	25.613198154128536	30.18978190665016	21.613232785863325
31	24.372949151955396	26.324447407381758	27.53136336481935	21.771240075843497
32	24.59978701483104	26.272499805196492	27.185695361944916	21.942017818027548
33	22.96841585787136	26.832018770400257	27.46642886208777	22.73313650964061
34	21.44656756218561	26.234837793612176	29.9774460827179	22.341148561484317
35	22.9904935888001	25.93765421944399	28.285252941532974	22.78659925022294
36	24.869914546194405	26.979203643258497	27.747162362230632	20.403719448316465
37	22.309330655145843	27.882875472939627	28.59823724469918	21.20955662721535
38	22.087904000831163	28.194561086051202	27.075739603986115	22.641795309131524
39	22.08920269088579	25.933758149280095	28.427243054172692	23.54979610566142
40	24.295893542047256	24.918182526558212	29.41359815066536	21.37232578072917
41	21.42643786633882	24.58896459770911	28.339797923827497	25.64479961212457
42	23.045904364464377	24.99826841326049	28.521830979818358	23.433996242456775
43	23.129669872988114	25.175972502402576	28.46252413399018	23.231833490619128
44	21.82297122968632	26.445009134120053	28.21317564350092	23.5188439926927
45	22.13119366931888	25.872070371685098	27.79910996441589	24.197625994580132
46	22.96755006450161	26.572064311131506	27.548246335529562	22.912139288837327
47	20.854797793958493	26.011896000900425	30.293893559363124	22.83941264577796
48	21.527086345572766	25.91730807525476	29.441087090155065	23.11451848901741
49	22.59893853732868	24.417321062155306	29.909914199877058	23.073826200638955
50	20.86778469450481	25.675102380065972	29.930260344066284	23.52685258136293
51	20.835100994796584	25.659085202725517	27.823785075453895	25.682028727024008
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	216.0
1	510.0
2	804.0
3	2610.5
4	4417.0
5	3004.0
6	1591.0
7	1564.0
8	1537.0
9	1529.5
10	1522.0
11	1545.0
12	1568.0
13	1519.0
14	1470.0
15	1419.0
16	1368.0
17	1361.5
18	1355.0
19	1470.5
20	1586.0
21	1834.5
22	2083.0
23	2308.5
24	2534.0
25	2992.5
26	3844.5
27	4238.0
28	5036.5
29	5835.0
30	6921.0
31	8007.0
32	9377.5
33	10748.0
34	11761.0
35	12774.0
36	14273.5
37	15773.0
38	16837.5
39	17902.0
40	19978.0
41	22054.0
42	24176.0
43	26298.0
44	28600.0
45	30902.0
46	32798.0
47	34694.0
48	37460.5
49	40227.0
50	38834.0
51	37441.0
52	34027.0
53	30613.0
54	28424.0
55	26235.0
56	24826.0
57	23417.0
58	22364.5
59	21312.0
60	19743.5
61	18175.0
62	16585.5
63	14996.0
64	12740.0
65	10484.0
66	9514.5
67	8545.0
68	7103.5
69	5662.0
70	4832.5
71	4003.0
72	3192.5
73	2382.0
74	2047.0
75	1377.5
76	1043.0
77	783.5
78	524.0
79	364.0
80	204.0
81	162.0
82	120.0
83	97.0
84	74.0
85	59.5
86	45.0
87	37.5
88	30.0
89	28.5
90	27.0
91	14.5
92	2.0
93	2.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	462004.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.55642882812875
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.91817581698888	25.13986434736024
2	9.1356405567029	6.131189442807348
3	3.598001085743903	3.622082020718858
4	2.0498453485638315	2.75141958191012
5	1.4072481430820896	2.3611111078425244
6	1.029372147047181	2.0725231914028095
7	0.7366931408858945	1.730455366521571
8	0.543715394580667	1.4596117552803274
9	0.42660858073959723	1.2883914429351584
>10	5.902259313857783	46.4835170399691
>50	0.22281735521765222	4.50583765633268
>100	0.027691174203349846	1.5427161780518441
>500	6.439807954267406E-4	0.12187868390828409
>1k	0.0012879615908534812	0.7894021849591522
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2498	0.5406879594116067	No Hit
GCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCGTCTTCTGC	1155	0.24999783551657562	No Hit
CCTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCGTCTTCTGC	564	0.1220768651353668	No Hit
CTGTCTCTTATACACATCTGACGCGCACATGTTCGTATGCCGTCTTCTGCT	488	0.10562679111003367	Illumina Single End Adapter 2 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.12077817508073523	0.0
2	0.0	0.0	0.0	0.7017255261859204	0.0
3	0.0	0.0	0.0	0.9274811473493736	0.0
4	0.0	0.0	0.0	1.2151409944502645	0.0
5	0.0	0.0	0.0	1.9705457095609562	0.0
6	0.0	0.0	0.0	2.453009064856581	0.0
7	0.0	0.0	0.0	2.873568194214769	0.0
8	0.0	0.0	0.0	3.696937688851179	0.0
9	0.0	0.0	0.0	3.972692877117947	0.0
10	0.0	0.0	0.0	4.65710253590878	0.0
11	0.0	0.0	0.0	5.608609449268838	0.0
12	0.0	0.0	0.0	6.312932355564021	0.0
13	0.0	0.0	0.0	6.66552670539649	0.0
14	0.0	0.0	0.0	6.82721361719812	0.0
15	0.0	0.0	0.0	7.082189764590783	0.0
16	0.0	0.0	0.0	7.621146137262881	0.0
17	0.0	0.0	0.0	8.248196985307487	0.0
18	0.0	0.0	0.0	8.941481026138302	0.0
19	0.0	0.0	0.0	9.362040155496489	0.0
20	0.0	0.0	0.0	9.772426212760063	0.0
21	0.0	0.0	0.0	10.302724651734618	0.0
22	0.0	0.0	0.0	10.825880295408698	0.0
23	2.164483424385936E-4	0.0	0.0	11.341027350412551	0.0
24	2.164483424385936E-4	0.0	0.0	11.751196959333685	0.0
25	2.164483424385936E-4	0.0	0.0	12.121756521588559	0.0
26	2.164483424385936E-4	0.0	0.0	12.4814936667215	0.0
27	2.164483424385936E-4	0.0	0.0	12.859628920961724	0.0
28	2.164483424385936E-4	0.0	0.0	13.211141029081999	0.0
29	2.164483424385936E-4	0.0	0.0	13.583215729733942	0.0
30	2.164483424385936E-4	0.0	0.0	14.0451164924979	0.0
31	2.164483424385936E-4	0.0	0.0	14.466324966883404	0.0
32	2.164483424385936E-4	0.0	0.0	14.828443043783171	0.0
33	6.493450273157808E-4	0.0	0.0	15.240127791101376	0.0
34	6.493450273157808E-4	0.0	0.0	15.653977021843966	0.0
35	6.493450273157808E-4	0.0	0.0	16.128431788469364	0.0
36	6.493450273157808E-4	0.0	0.0	16.521934875022726	0.0
37	6.493450273157808E-4	0.0	0.0	16.945740729517492	0.0
38	6.493450273157808E-4	0.0	0.0	17.39422169505026	0.0
39	6.493450273157808E-4	0.0	0.0	17.95871897213011	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACCTA	20	7.0289004E-4	45.000004	17
ATTCGAT	20	7.0289004E-4	45.000004	16
ACGGGTC	20	7.0289004E-4	45.000004	5
TTTGCGC	45	3.8380676E-10	45.000004	13
CGTCCCA	20	7.0289004E-4	45.000004	23
CCTGCGA	20	7.0289004E-4	45.000004	42
CCGTTAC	35	1.2098099E-7	45.000004	45
CTACTAT	25	3.8871724E-5	45.0	23
CATAGCG	25	3.8871724E-5	45.0	21
TGTTACG	25	3.8871724E-5	45.0	1
TATAGCG	25	3.8871724E-5	45.0	1
CGGCTAC	25	3.8871724E-5	45.0	25
ATACCGG	25	3.8871724E-5	45.0	2
AAGTCGT	25	3.8871724E-5	45.0	18
CGTTTTT	1335	0.0	42.303375	1
TAGCATA	105	0.0	40.714287	30
TCGATCA	50	1.0786607E-9	40.5	17
CCCACGA	50	1.0786607E-9	40.5	39
CCCCACG	50	1.0786607E-9	40.5	38
CTCGATC	50	1.0786607E-9	40.5	16
>>END_MODULE