##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936745.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	258314
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.131096262688047	31.0	31.0	33.0	28.0	34.0
2	31.18902575934715	31.0	31.0	34.0	28.0	34.0
3	30.85800614755685	31.0	31.0	34.0	27.0	34.0
4	34.84397671051511	35.0	35.0	37.0	32.0	37.0
5	35.15634460385422	35.0	35.0	37.0	33.0	37.0
6	35.16124561580092	36.0	35.0	37.0	32.0	37.0
7	31.519716314253195	35.0	35.0	37.0	0.0	37.0
8	33.37254271932609	37.0	35.0	37.0	17.0	37.0
9	36.563504107404164	39.0	35.0	39.0	32.0	39.0
10	36.19182854974953	37.0	35.0	39.0	32.0	39.0
11	36.44472618595972	38.0	35.0	39.0	32.0	39.0
12	36.44735864103378	38.0	35.0	39.0	32.0	39.0
13	36.28128169592047	38.0	35.0	39.0	32.0	39.0
14	37.324229426202216	39.0	36.0	40.0	32.0	41.0
15	37.57172278699567	39.0	36.0	41.0	33.0	41.0
16	37.56455321817633	39.0	36.0	41.0	33.0	41.0
17	37.45258096734982	39.0	36.0	40.0	32.0	41.0
18	37.33339656387188	39.0	36.0	40.0	32.0	41.0
19	37.087335568339306	39.0	36.0	40.0	32.0	41.0
20	37.005404275416744	39.0	35.0	40.0	32.0	41.0
21	36.98167733843307	39.0	35.0	40.0	32.0	41.0
22	37.050264406884644	39.0	35.0	40.0	32.0	41.0
23	37.10969208018148	39.0	35.0	40.0	32.0	41.0
24	37.025503844158656	39.0	35.0	40.0	32.0	41.0
25	36.72331735794421	38.0	35.0	40.0	31.0	41.0
26	36.70265258561286	38.0	35.0	40.0	31.0	41.0
27	36.70032208862082	38.0	35.0	40.0	31.0	41.0
28	36.70814589995122	38.0	35.0	40.0	31.0	41.0
29	36.65219461585512	38.0	35.0	40.0	31.0	41.0
30	36.35620601283709	38.0	35.0	40.0	30.0	41.0
31	36.28472324380405	38.0	35.0	40.0	30.0	41.0
32	35.990085709640205	38.0	35.0	40.0	30.0	41.0
33	35.68324210069915	38.0	35.0	40.0	28.0	41.0
34	35.55398468530548	38.0	35.0	40.0	27.0	41.0
35	35.33063248604412	38.0	35.0	40.0	25.0	41.0
36	35.176889367204254	38.0	35.0	40.0	25.0	41.0
37	35.02311140704724	38.0	34.0	40.0	24.0	41.0
38	34.901600377834725	38.0	34.0	40.0	23.0	41.0
39	34.867664934924164	38.0	34.0	40.0	23.0	41.0
40	34.75119428292698	38.0	34.0	40.0	23.0	41.0
41	34.64686389433015	38.0	34.0	40.0	23.0	41.0
42	34.64127379855525	38.0	34.0	40.0	23.0	41.0
43	34.55861083797239	38.0	34.0	40.0	23.0	41.0
44	34.32174407891171	38.0	34.0	40.0	21.0	41.0
45	34.24128386382465	38.0	33.0	40.0	22.0	41.0
46	34.09646399343435	37.0	33.0	40.0	20.0	41.0
47	34.03087327825824	37.0	33.0	40.0	20.0	41.0
48	33.98235093723143	37.0	33.0	40.0	20.0	41.0
49	33.89488374613842	37.0	33.0	40.0	20.0	41.0
50	33.73862430994836	37.0	33.0	40.0	20.0	41.0
51	32.02032023041724	35.0	30.0	39.0	14.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	10.0
9	16.0
10	17.0
11	14.0
12	5.0
13	4.0
14	10.0
15	13.0
16	27.0
17	70.0
18	142.0
19	243.0
20	438.0
21	799.0
22	1159.0
23	1786.0
24	2613.0
25	3789.0
26	4836.0
27	5190.0
28	5374.0
29	5489.0
30	6142.0
31	7440.0
32	9894.0
33	13232.0
34	21540.0
35	23470.0
36	23750.0
37	35773.0
38	48523.0
39	36500.0
40	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.478696470187444	19.624178325603722	24.481832188731545	16.415293015477285
2	34.3860572791254	23.748616025457387	25.811609126876593	16.053717568540613
3	29.896172874873216	24.094319316800483	31.06219562238206	14.94731218594424
4	27.185518400086718	23.812491773577893	31.236402208165252	17.765587618170134
5	24.69436422338704	29.37897287797022	29.68828634917194	16.238376549470797
6	25.594431583266875	34.051967760167855	28.397221985645377	11.95637867091989
7	66.98475498811524	16.403292117345554	13.265250818770955	3.346702075768251
8	76.86304265351471	6.584234691112368	12.702369983818143	3.8503526715547745
9	72.18656363960142	6.756892773910822	14.48856817671516	6.567975409772603
10	40.82782969564174	22.834225012968712	22.797835192827336	13.540110098562213
11	30.91353933584707	24.548030691329156	28.610528271793246	15.927901701030528
12	28.389866596467865	21.802147773639835	31.753989330814434	18.053996299077866
13	22.565946870862593	26.47978816479169	33.140286627902476	17.813978336443242
14	19.185564855176256	29.436267488405583	32.37184202172549	19.00632563469266
15	18.77288880974318	25.042003143461063	39.44308090153844	16.742027145257325
16	20.674063349257104	23.475305248650866	36.81604558792787	19.034585814164156
17	21.32366035135533	23.29142051921305	32.672638726511146	22.712280402920477
18	22.142044178790155	24.49034895514761	34.45225578172302	18.915351084339214
19	22.63717800816061	25.449646554193734	32.20305519638889	19.710120241256764
20	23.956115425412484	25.07607020912533	32.433395015368895	18.534419350093298
21	22.442840883575805	26.64973636736685	32.957950401449395	17.94947234760795
22	22.148625316475297	23.240707046462834	31.688565079709193	22.92210255735268
23	19.53668790696594	25.97729894624372	33.02414890404701	21.461864242743328
24	21.379793584552136	23.8020393784309	35.187407573728095	19.630759463288864
25	18.345501985955078	26.50572558978607	33.57115758340624	21.577614840852604
26	19.49526545212416	27.25713666313092	33.25913423198123	19.98846365276369
27	20.021369341189406	28.642272582980404	33.002082736514474	18.334275339315717
28	18.50461066763706	26.446882476365975	35.35542014757234	19.69308670842463
29	19.875422934877708	23.732356744117624	35.49478541619889	20.897434904805777
30	19.741864552443925	26.840976485982175	34.61833272683633	18.798826234737568
31	23.512469320284616	25.367963021748725	30.86011598287356	20.259451675093104
32	23.626671415409152	26.29358068087676	29.783519282733415	20.296228620980667
33	21.706140588585985	26.587409122231083	29.85591179726999	21.850538491912943
34	21.597745379654217	26.56805283492184	30.54151149376341	21.292690291660538
35	19.372933716329737	26.685351936015856	30.78965909706791	23.152055250586496
36	23.585636086313556	26.35552080026634	30.72268634297793	19.336156770442177
37	20.766973528341477	26.371392955859925	32.722965073515184	20.13866844228342
38	20.728648079469174	29.319742638803937	27.85485881524037	22.096750466486526
39	20.68490287015028	26.623411816626273	31.422222566333996	21.269462746889445
40	21.307401070015565	26.86304265351471	31.179881849222262	20.649674427247458
41	19.00555138320029	27.09067259227142	30.22522975913036	23.678546265397927
42	22.116106753795766	25.142655837469125	29.60040880478797	23.140828603947135
43	22.509039386173416	25.567719906780123	29.403361799979873	22.51987890706659
44	20.907887299952773	25.889808527605933	30.080831855803403	23.121472316637888
45	19.900198982633537	27.90363665925966	29.348777069767802	22.847387288339
46	22.361931602623166	27.14216031651401	30.343690237462933	20.152217843399896
47	19.89323071920221	26.09343667009918	31.679274061800754	22.334058548897854
48	20.87498161152706	25.2200809867061	30.941025263826194	22.963912137940646
49	21.227266040555293	23.415687883738396	32.85226507274093	22.504781002965384
50	20.14718520869949	25.0946522449422	32.01955759269726	22.73860495366105
51	19.329188507010848	26.31758247714022	28.957780066121074	25.39544894972785
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	109.0
1	192.0
2	275.0
3	2693.5
4	5112.0
5	3549.0
6	1986.0
7	1888.0
8	1790.0
9	1798.5
10	1807.0
11	1797.5
12	1788.0
13	1683.0
14	1578.0
15	1496.5
16	1415.0
17	1351.5
18	1288.0
19	1178.5
20	1069.0
21	1024.0
22	979.0
23	1101.0
24	1223.0
25	1497.0
26	2044.5
27	2318.0
28	2758.0
29	3198.0
30	3852.5
31	4507.0
32	5061.0
33	5615.0
34	6350.5
35	7086.0
36	7365.0
37	7644.0
38	9226.5
39	10809.0
40	11819.5
41	12830.0
42	15463.0
43	18096.0
44	19652.0
45	21208.0
46	23964.0
47	26720.0
48	25990.0
49	25260.0
50	23773.5
51	22287.0
52	19577.0
53	16867.0
54	15179.5
55	13492.0
56	11945.0
57	10398.0
58	9209.0
59	8020.0
60	7150.5
61	6281.0
62	5603.0
63	4925.0
64	4127.5
65	3330.0
66	2757.5
67	2185.0
68	1773.5
69	1362.0
70	1014.5
71	667.0
72	545.0
73	423.0
74	332.5
75	195.0
76	148.0
77	110.0
78	72.0
79	72.0
80	72.0
81	55.0
82	38.0
83	26.5
84	15.0
85	11.5
86	8.0
87	4.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	258314.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.31438481847673
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.55170901756155	27.033765107582248
2	8.691982197276973	6.1390400829997605
3	3.408169082019688	3.610721834666336
4	1.8646817653636183	2.634003577041895
5	1.3045098770033545	2.303398189800011
6	0.9120606871149504	1.9325317249548997
7	0.6917191028479972	1.7099344208985963
8	0.5327662186753196	1.505144901166797
9	0.43958694174650853	1.3971368179812167
>10	5.352875402863344	43.176521597745385
>50	0.21486045032996426	4.442267937471449
>100	0.029598123259739973	1.598055080251167
>500	0.0021924535747955536	0.5892053856933809
>1k	0.0032886803621933304	1.9282733417468663
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2837	1.0982757419264926	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCCG	1127	0.436290715950355	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGC	1017	0.3937068838700187	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCCGTCTTC	801	0.3100877226940855	No Hit
GCTGTCTCTTATACACATCTGACGCGTGCATACTCGTATGCCGTCTTCTGC	721	0.2791176629992954	TruSeq Adapter, Index 20 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1262029932562695	0.0
2	0.0	0.0	0.0	0.7963176599022894	0.0
3	0.0	0.0	0.0	1.0088496945577863	0.0
4	0.0	0.0	0.0	1.3746835247024938	0.0
5	0.0	0.0	0.0	2.455151482304482	0.0
6	0.0	0.0	0.0	2.860085012813862	0.0
7	0.0	0.0	0.0	3.390447285087142	0.0
8	0.0	0.0	0.0	4.312967938245701	0.0
9	0.0	0.0	0.0	4.579697577367081	0.0
10	0.0	0.0	0.0	5.756946971515288	0.0
11	0.0	0.0	0.0	6.689145768328468	0.0
12	0.0	0.0	0.0	7.861749653522457	0.0
13	0.0	0.0	0.0	8.1652562385314	0.0
14	0.0	0.0	0.0	8.30423438141177	0.0
15	0.0	0.0	0.0	8.608902343659267	0.0
16	0.0	0.0	0.0	9.14352299914058	0.0
17	0.0	0.0	0.0	9.752858923635575	0.0
18	0.0	0.0	0.0	10.460137661915343	0.0
19	0.0	0.0	0.0	10.901073886819917	0.0
20	0.0	0.0	0.0	11.304846040090743	0.0
21	0.0	0.0	0.0	11.820884659755182	0.0
22	0.0	0.0	0.0	12.325309507034074	0.0
23	0.0	0.0	0.0	12.873092437885674	0.0
24	0.0	0.0	0.0	13.312867285551693	0.0
25	0.0	0.0	0.0	13.700380157482753	0.0
26	0.0	0.0	0.0	14.06931099359694	0.0
27	0.0	0.0	0.0	14.419659793894253	0.0
28	0.0	0.0	0.0	14.781235240830927	0.0
29	0.0	0.0	0.0	15.211331944842323	0.0
30	3.8712574618487576E-4	0.0	0.0	15.667753199594292	0.0
31	3.8712574618487576E-4	0.0	0.0	16.148176250609723	0.0
32	3.8712574618487576E-4	0.0	0.0	16.5685948109665	0.0
33	3.8712574618487576E-4	0.0	0.0	16.972366964237324	0.0
34	3.8712574618487576E-4	0.0	0.0	17.373429237284856	0.0
35	3.8712574618487576E-4	0.0	0.0	17.79384779764163	0.0
36	3.8712574618487576E-4	0.0	0.0	18.24872054940886	0.0
37	3.8712574618487576E-4	0.0	0.0	18.64165318178651	0.0
38	3.8712574618487576E-4	0.0	0.0	19.064007370874208	0.0
39	3.8712574618487576E-4	0.0	0.0	19.44842323683579	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTTTT	25	3.8823822E-5	45.000004	1
GGCGAAT	25	3.8823822E-5	45.000004	8
CTTAACG	20	7.023122E-4	45.0	44
GCGAATA	20	7.023122E-4	45.0	9
GGCACCT	20	7.023122E-4	45.0	8
TAGCGTC	20	7.023122E-4	45.0	35
TGGGTCG	20	7.023122E-4	45.0	1
AAGCGTG	20	7.023122E-4	45.0	34
TACGGGT	20	7.023122E-4	45.0	4
CACGAAG	20	7.023122E-4	45.0	1
CGACAAT	35	1.207045E-7	45.0	20
TGCGATA	20	7.023122E-4	45.0	1
CTCATAA	20	7.023122E-4	45.0	11
TTAACGA	20	7.023122E-4	45.0	45
GAGCCGC	20	7.023122E-4	45.0	40
CGTAAGG	20	7.023122E-4	45.0	2
AATAGCG	20	7.023122E-4	45.0	33
CGCGTCG	20	7.023122E-4	45.0	15
CGGAGAC	20	7.023122E-4	45.0	28
TCGCGTC	20	7.023122E-4	45.0	14
>>END_MODULE