##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936743.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	443080
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.110088471607835	31.0	31.0	33.0	28.0	34.0
2	31.154270109235352	31.0	31.0	34.0	28.0	34.0
3	30.9064390177846	31.0	31.0	34.0	27.0	34.0
4	34.878015256838495	35.0	35.0	37.0	32.0	37.0
5	35.14231064367608	35.0	35.0	37.0	33.0	37.0
6	35.13271643946917	36.0	35.0	37.0	32.0	37.0
7	31.09357226685926	35.0	35.0	37.0	0.0	37.0
8	33.10744335108784	36.0	35.0	37.0	17.0	37.0
9	36.335104270109234	39.0	35.0	39.0	32.0	39.0
10	36.1230635551142	37.0	35.0	39.0	32.0	39.0
11	36.36543513586711	38.0	35.0	39.0	32.0	39.0
12	36.36758824591496	38.0	35.0	39.0	32.0	39.0
13	36.2414281845265	38.0	35.0	39.0	32.0	39.0
14	37.28480409858265	39.0	36.0	40.0	32.0	41.0
15	37.50986277873071	39.0	36.0	41.0	32.0	41.0
16	37.437733592127834	39.0	36.0	41.0	32.0	41.0
17	37.33377719599169	39.0	36.0	40.0	32.0	41.0
18	37.25696939604586	39.0	36.0	40.0	32.0	41.0
19	37.05837094881286	39.0	35.0	40.0	32.0	41.0
20	37.052712828383136	39.0	35.0	40.0	32.0	41.0
21	37.03750338539316	39.0	35.0	40.0	32.0	41.0
22	37.13639749029521	39.0	35.0	40.0	32.0	41.0
23	37.20241039992778	39.0	35.0	40.0	32.0	41.0
24	37.11608513135325	39.0	35.0	40.0	32.0	41.0
25	36.78403899972917	38.0	35.0	40.0	31.0	41.0
26	36.77637221269297	39.0	35.0	40.0	31.0	41.0
27	36.80701002076375	39.0	35.0	40.0	31.0	41.0
28	36.810580482079985	39.0	35.0	40.0	31.0	41.0
29	36.812045228852575	39.0	35.0	40.0	31.0	41.0
30	36.55538503204839	38.0	35.0	40.0	31.0	41.0
31	36.526586620926246	38.0	35.0	40.0	31.0	41.0
32	36.32009795070868	38.0	35.0	40.0	30.0	41.0
33	36.15698519454726	38.0	35.0	40.0	30.0	41.0
34	36.07671977972375	38.0	35.0	40.0	30.0	41.0
35	35.95223435948361	38.0	35.0	40.0	29.0	41.0
36	35.88196939604586	38.0	35.0	40.0	29.0	41.0
37	35.84143946917035	38.0	35.0	40.0	29.0	41.0
38	35.67541978875147	38.0	35.0	40.0	28.0	41.0
39	35.66378532093527	38.0	35.0	40.0	28.0	41.0
40	35.480816105443715	38.0	34.0	40.0	27.0	41.0
41	35.30912250609371	38.0	34.0	40.0	26.0	41.0
42	35.312871264782885	38.0	34.0	40.0	27.0	41.0
43	35.26351223255394	38.0	34.0	40.0	27.0	41.0
44	35.0695292046583	38.0	34.0	40.0	26.0	41.0
45	34.95067933556017	38.0	34.0	40.0	25.0	41.0
46	34.80726505371491	37.0	34.0	40.0	24.0	41.0
47	34.72990882007764	37.0	34.0	40.0	24.0	41.0
48	34.655989888959105	37.0	34.0	40.0	24.0	41.0
49	34.556890403538866	37.0	34.0	40.0	24.0	41.0
50	34.412530468538414	37.0	33.0	40.0	24.0	41.0
51	32.75373973097409	35.0	31.0	39.0	19.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	23.0
9	36.0
10	36.0
11	18.0
12	24.0
13	18.0
14	18.0
15	32.0
16	58.0
17	113.0
18	234.0
19	383.0
20	659.0
21	939.0
22	1487.0
23	2184.0
24	3244.0
25	4559.0
26	6017.0
27	6983.0
28	7828.0
29	8873.0
30	10882.0
31	13397.0
32	17299.0
33	24167.0
34	37421.0
35	40295.0
36	43754.0
37	64028.0
38	84157.0
39	63910.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.138394872257834	20.880653606572178	25.03204838855286	12.948903132617135
2	34.3964972465469	23.878983479281395	24.458562787758417	17.265956486413288
3	32.32373386295929	23.14841563600253	28.12291234088652	16.404938160151666
4	28.81646655231561	25.235171977972374	27.231425476211975	18.716935993500044
5	25.320709578405705	29.247991333393518	26.416222804008306	19.01507628419247
6	25.95264963437754	34.73887334115736	25.738467093978514	13.570009930486593
7	67.75932111582559	18.058589870903674	10.558589870903674	3.623499142367067
8	78.05520447774668	6.6741897625710935	10.691748668412025	4.5788570912702
9	71.44556287803557	8.573846709397852	13.186557732237972	6.794032680328608
10	41.21513045048298	25.608467996750022	20.367879389726458	12.808522163040534
11	32.979597363907196	24.263112756161416	25.662859980139025	17.094429899792363
12	29.971562697481268	22.33953236435858	29.89121603322199	17.797688904938163
13	24.762345400379164	25.562200956937797	30.456350997562513	19.21910264512052
14	19.888282025819265	29.16583912611718	30.611176311275617	20.334702536787937
15	19.51430892841022	25.784508440913605	36.06098221540128	18.640200415274894
16	22.4435767807168	24.40123679696669	34.01281935542114	19.142367066895368
17	21.771463392615328	25.1816827660919	29.359257921819985	23.68759591947278
18	23.479507086756342	25.744109415906834	30.97589600072222	19.800487496614608
19	25.117585988986185	26.205199963889143	28.244786494538232	20.43242755258644
20	26.753859348198972	25.306716620023472	28.958427372032137	18.98099665974542
21	24.262435677530018	26.525458156540584	29.95982666787036	19.252279498059043
22	22.5130901868737	24.88241401101381	29.81132075471698	22.793175047395504
23	21.99580211248533	26.509885348018415	30.193870181457072	21.30044235803918
24	23.47183352893383	24.343910806174957	31.272682134151847	20.911573530739368
25	21.459781529294936	25.79737293491017	30.479597363907196	22.263248171887696
26	20.236751828112304	27.281754987812583	29.26266137040715	23.21883181366796
27	21.11650266317595	26.576013361018326	29.954184345942043	22.35329962986368
28	18.752144082332762	28.19919653335741	31.52839216394331	21.520267220366527
29	21.60377358490566	26.14223165116909	30.155502392344495	22.098492371580754
30	21.243342060124583	26.779814029069243	30.321386657037102	21.655457253769068
31	24.276880021666518	26.722036652523247	27.57741265685655	21.423670668953687
32	25.081475128644943	26.754762119707504	28.084544551773945	20.079218199873612
33	22.916629051187147	27.904441635821975	27.893382684842464	21.285546628148417
34	21.665387740362917	26.80418885979958	29.86210165207186	21.66832174776564
35	20.879976527940777	26.36408774939063	29.799584725106076	22.956350997562517
36	22.148821883181366	28.76433149769793	28.303918028347024	20.782928590773675
37	22.47968764105805	28.046402455538505	28.963166922451926	20.51074298095152
38	21.028256748217025	29.81177214047125	27.145436490024377	22.014534621287353
39	24.357000993048658	26.161189852848242	27.78482441094159	21.696984743161508
40	23.36733772682134	24.713370046041348	30.0406247178839	21.878667509253408
41	19.620158887785504	26.42863591225061	29.61022840119166	24.340976798772232
42	21.546898979868196	25.875011284643858	28.864087749390627	23.71400198609732
43	22.358264873160604	25.563555114200597	28.91577141825404	23.16240859438476
44	21.591134783786224	26.827435226144264	27.990656314886703	23.59077367518281
45	21.608287442448315	26.78590773675183	26.990385483434142	24.61541933736571
46	22.47675363365532	27.07547169811321	27.86155998916674	22.586214679064728
47	20.911573530739368	26.63266227317866	30.04920104721495	22.406563148867022
48	21.406518010291595	24.689220908188137	30.98424663717613	22.920014444344137
49	21.886341067075925	24.472781439017783	30.831452559357224	22.809424934549067
50	20.065450934368513	25.688588968132166	29.77114742258734	24.47481267491198
51	19.737519183894555	25.9124763022479	28.710842285817463	25.639162228040085
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	149.0
1	490.0
2	831.0
3	3390.5
4	5950.0
5	4066.5
6	2183.0
7	2018.0
8	1853.0
9	1797.0
10	1741.0
11	1658.5
12	1576.0
13	1599.5
14	1623.0
15	1603.0
16	1583.0
17	1445.0
18	1307.0
19	1367.5
20	1428.0
21	1661.5
22	1895.0
23	2061.5
24	2228.0
25	3046.0
26	4386.0
27	4908.0
28	5607.5
29	6307.0
30	7809.5
31	9312.0
32	10374.0
33	11436.0
34	12096.0
35	12756.0
36	13428.0
37	14100.0
38	15897.5
39	17695.0
40	19585.0
41	21475.0
42	24169.5
43	26864.0
44	28236.5
45	29609.0
46	33543.0
47	37477.0
48	37211.0
49	36945.0
50	35562.5
51	34180.0
52	30809.5
53	27439.0
54	25731.5
55	24024.0
56	22642.5
57	21261.0
58	20294.5
59	19328.0
60	18682.5
61	18037.0
62	16373.5
63	14710.0
64	11996.5
65	9283.0
66	7724.5
67	6166.0
68	5275.0
69	4384.0
70	3530.0
71	2676.0
72	2322.5
73	1969.0
74	1622.5
75	926.5
76	577.0
77	457.5
78	338.0
79	247.0
80	156.0
81	112.0
82	68.0
83	48.0
84	28.0
85	25.5
86	23.0
87	33.5
88	44.0
89	28.0
90	12.0
91	7.5
92	3.0
93	3.0
94	3.0
95	1.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	443080.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.78558067040211
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.89842135433133	24.555882353981982
2	9.105648180975967	5.970679259873757
3	3.5992954381762523	3.540149728348135
4	1.9937787618736484	2.614687777453718
5	1.3493675509533176	2.211989934790146
6	0.9801459284224693	1.9280792043036625
7	0.6896671269450207	1.5827796058326304
8	0.5622727391993854	1.4747550599831536
9	0.4704649219167157	1.388201908508542
>10	6.043792559798879	46.052099170233376
>50	0.2720234255062676	5.341584672138601
>100	0.031678677400729896	1.7362746694034086
>500	0.0013773338000317346	0.39692729113279485
>1k	0.002066000700047602	1.2059093640160734
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3175	0.7165748848966327	No Hit
GCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTCTGC	1137	0.256612801299991	TruSeq Adapter, Index 14 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCG	1029	0.23223797056964882	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGC	993	0.22411302699286811	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCAGAGTTTCGTATGCCGTCTTC	765	0.17265505100659023	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1166832174776564	0.0
2	0.0	0.0	0.0	0.7294393788932021	0.0
3	0.0	0.0	0.0	0.9312088110499233	0.0
4	0.0	0.0	0.0	1.2478559176672384	0.0
5	0.0	0.0	0.0	2.2144985104270107	0.0
6	0.0	0.0	0.0	2.638349733682405	0.0
7	0.0	0.0	0.0	3.1025999819445698	0.0
8	0.0	0.0	0.0	3.941049020492913	0.0
9	0.0	0.0	0.0	4.218651259366254	0.0
10	0.0	0.0	0.0	5.11374921007493	0.0
11	0.0	0.0	0.0	6.049923264421775	0.0
12	0.0	0.0	0.0	7.01521169991875	0.0
13	0.0	0.0	0.0	7.355330865757876	0.0
14	0.0	0.0	0.0	7.502708314525593	0.0
15	0.0	0.0	0.0	7.811230477566128	0.0
16	0.0	0.0	0.0	8.37929944930938	0.0
17	0.0	0.0	0.0	9.030649092714635	0.0
18	0.0	0.0	0.0	9.775209894375733	0.0
19	0.0	0.0	0.0	10.203123589419517	0.0
20	0.0	0.0	0.0	10.617270018958202	0.0
21	0.0	0.0	0.0	11.137943486503566	0.0
22	0.0	0.0	0.0	11.704206915229756	0.0
23	0.0	0.0	0.0	12.246546898979869	0.0
24	0.0	0.0	0.0	12.652794077818903	0.0
25	0.0	0.0	0.0	13.02586440371942	0.0
26	0.0	0.0	0.0	13.389229935903224	0.0
27	0.0	0.0	0.0	13.773359212783244	0.0
28	2.2569287713279768E-4	0.0	0.0	14.175092534079624	0.0
29	2.2569287713279768E-4	0.0	0.0	14.548614245734404	0.0
30	2.2569287713279768E-4	0.0	0.0	15.004513857542657	0.0
31	2.2569287713279768E-4	0.0	0.0	15.411212422135957	0.0
32	2.2569287713279768E-4	0.0	0.0	15.798952785050103	0.0
33	2.2569287713279768E-4	0.0	0.0	16.209036742800397	0.0
34	2.2569287713279768E-4	0.0	0.0	16.591360476663358	0.0
35	2.2569287713279768E-4	0.0	0.0	17.016114471427283	0.0
36	2.2569287713279768E-4	0.0	0.0	17.426198429177575	0.0
37	2.2569287713279768E-4	0.0	0.0	17.831994222262345	0.0
38	2.2569287713279768E-4	0.0	0.0	18.279543197616682	0.0
39	2.2569287713279768E-4	0.0	0.0	18.70046041346935	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	25	3.8869126E-5	45.0	25
AAACTCG	20	7.0285884E-4	45.0	42
CGGGTAC	35	1.2096643E-7	45.0	6
CCGGGTA	35	1.2096643E-7	45.0	5
CGAACAC	25	3.8869126E-5	45.0	32
TCCGCAA	25	3.8869126E-5	45.0	33
CCTACGA	20	7.0285884E-4	45.0	27
TACGGGT	20	7.0285884E-4	45.0	4
CTGACGT	20	7.0285884E-4	45.0	38
ATCCGTA	25	3.8869126E-5	45.0	34
CTTGCGA	25	3.8869126E-5	45.0	42
ACAATCC	20	7.0285884E-4	45.0	24
ATCCATA	25	3.8869126E-5	45.0	29
CGAATGG	20	7.0285884E-4	45.0	2
TAGGGCG	70	0.0	45.0	5
GCGATCT	20	7.0285884E-4	45.0	9
ACGTCTT	20	7.0285884E-4	45.0	41
TATAGCG	35	1.2096643E-7	45.0	1
CGGTCTA	25	3.8869126E-5	45.0	31
CGCAATC	35	1.2096643E-7	45.0	20
>>END_MODULE