##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936740.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	419767
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.219426491363066	31.0	31.0	33.0	28.0	34.0
2	31.258224205332958	31.0	31.0	34.0	28.0	34.0
3	31.021257030686073	31.0	31.0	34.0	28.0	34.0
4	34.98539666052834	35.0	35.0	37.0	32.0	37.0
5	35.238915874759094	35.0	35.0	37.0	33.0	37.0
6	35.224152922931054	36.0	35.0	37.0	33.0	37.0
7	30.89419368363878	35.0	35.0	37.0	0.0	37.0
8	33.054851858292814	36.0	35.0	37.0	17.0	37.0
9	36.39542174587331	39.0	35.0	39.0	32.0	39.0
10	36.216636848537405	37.0	35.0	39.0	32.0	39.0
11	36.491646556303856	38.0	35.0	39.0	32.0	39.0
12	36.557211500665844	38.0	35.0	39.0	32.0	39.0
13	36.49070794035739	39.0	35.0	39.0	32.0	39.0
14	37.549881243642304	39.0	36.0	41.0	33.0	41.0
15	37.771999704598024	39.0	37.0	41.0	33.0	41.0
16	37.717281253647855	39.0	36.0	41.0	33.0	41.0
17	37.62322669480926	39.0	36.0	41.0	33.0	41.0
18	37.51536209373295	39.0	36.0	40.0	33.0	41.0
19	37.30739195791951	39.0	36.0	40.0	32.0	41.0
20	37.300814499472324	39.0	35.0	40.0	32.0	41.0
21	37.30037854333475	39.0	35.0	40.0	32.0	41.0
22	37.34866961909822	39.0	35.0	40.0	32.0	41.0
23	37.40630397339476	39.0	35.0	40.0	33.0	41.0
24	37.33488101732628	39.0	35.0	41.0	32.0	41.0
25	37.005405379651094	39.0	35.0	40.0	32.0	41.0
26	36.99598825062475	39.0	35.0	40.0	32.0	41.0
27	37.046904592309545	39.0	35.0	40.0	32.0	41.0
28	37.06812827115996	39.0	35.0	40.0	32.0	41.0
29	37.070946501273326	39.0	35.0	40.0	32.0	41.0
30	36.85392610662582	39.0	35.0	40.0	31.0	41.0
31	36.80691192971339	39.0	35.0	40.0	31.0	41.0
32	36.65239763964294	39.0	35.0	40.0	31.0	41.0
33	36.495174704062016	39.0	35.0	40.0	30.0	41.0
34	36.36358741873468	39.0	35.0	40.0	30.0	41.0
35	36.29542341346509	39.0	35.0	40.0	30.0	41.0
36	36.23880390788223	39.0	35.0	40.0	30.0	41.0
37	36.08576662767678	39.0	35.0	40.0	30.0	41.0
38	36.00607718091227	39.0	35.0	40.0	29.0	41.0
39	35.96520688858343	39.0	35.0	40.0	29.0	41.0
40	35.87408252673507	38.0	35.0	40.0	29.0	41.0
41	35.752653257640546	38.0	35.0	40.0	29.0	41.0
42	35.73362603539583	38.0	35.0	40.0	28.0	41.0
43	35.6679562709789	38.0	35.0	40.0	28.0	41.0
44	35.45543122732373	38.0	35.0	40.0	27.0	41.0
45	35.408860153370796	38.0	34.0	40.0	27.0	41.0
46	35.35700281346556	38.0	34.0	40.0	27.0	41.0
47	35.319951306319936	38.0	34.0	40.0	27.0	41.0
48	35.23657409944088	38.0	34.0	40.0	27.0	41.0
49	35.11507812667504	38.0	34.0	40.0	26.0	41.0
50	34.95158981053775	37.0	34.0	40.0	26.0	41.0
51	33.31579900278011	36.0	32.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	23.0
9	44.0
10	43.0
11	44.0
12	13.0
13	15.0
14	17.0
15	28.0
16	48.0
17	99.0
18	160.0
19	333.0
20	541.0
21	944.0
22	1403.0
23	1836.0
24	2736.0
25	3955.0
26	5185.0
27	6137.0
28	6390.0
29	7198.0
30	8672.0
31	11090.0
32	14632.0
33	20616.0
34	32154.0
35	36638.0
36	41986.0
37	63327.0
38	85035.0
39	68421.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.05134515100043	21.6408150235726	23.96329392258086	13.344545902846102
2	35.149023148556225	23.430617461591787	25.137754992650684	16.282604397201304
3	32.35866564070067	22.563231507002694	28.94153184981192	16.13657100248471
4	29.561875993110466	24.831871014157855	26.78390630992908	18.8223466828026
5	25.992991349963194	29.05373695407214	25.73070298522752	19.222568710737146
6	26.14355106523381	35.5764030998149	24.935738159502773	13.344307675448523
7	67.07149442428776	19.28974883685473	10.328110594687052	3.3106461441704567
8	78.9835789854848	6.025485566993118	10.863169329651926	4.127766117870152
9	72.36657479030033	8.65837476504823	12.56625699495196	6.408793449699476
10	40.76904568486803	25.20827030233439	19.987278656969224	14.035405355828354
11	30.921439751100017	26.681706756367223	25.060331088437156	17.336522404095607
12	29.495410549185618	22.95964189657596	29.608330335638584	17.936617218599842
13	24.5693444220246	24.666779427634854	31.427434743560116	19.336441406780427
14	20.068275972146452	28.69210776454557	30.07668539928103	21.16293086402695
15	19.34716163967153	26.286725731179438	35.37915081461859	18.986961814530442
16	21.929308402042086	24.59912284672211	34.61158213961555	18.859986611620254
17	22.13323105437064	24.51741084935214	30.612458816438643	22.73689927983858
18	22.38551386840795	25.745949538672647	31.101539663670557	20.766996929248847
19	24.174125169439236	26.8339340634209	28.95630194846188	20.03563881867798
20	25.733799941396065	26.1747588543168	28.769055213963934	19.322385990323205
21	25.198264751636028	25.538929930175552	29.714817982356877	19.547987335831547
22	22.92843410749297	24.938120433478574	28.760240800253474	23.373204658774988
23	22.511059706932656	26.48898079172493	29.550202850629038	21.449756650713372
24	23.292445570995337	24.23773188459312	31.119168491091486	21.350654053320056
25	21.81233874983026	26.544726002758672	29.384396581913297	22.258538665497767
26	20.94638215962665	28.272827544804617	30.17602622407193	20.604764071496806
27	22.12822827902146	27.991480988262534	30.58434798352419	19.295942749191813
28	20.738886096334394	27.39281553814378	30.982425964880516	20.885872400641308
29	20.580941331738796	26.95185662522304	31.870775930456656	20.596426112581504
30	22.47008459454888	25.95273091977216	31.00767806902401	20.569506416654953
31	22.99728182539361	27.483818404019374	28.064140344524464	21.45475942606255
32	24.0704962514919	28.055564158211578	28.468650465615447	19.405289124681076
33	24.246784525701162	27.342073102459217	27.638189757651265	20.77295261418835
34	21.956704552763796	28.33667248735608	29.08732701713093	20.61929594274919
35	23.042068576138668	25.46388829993782	29.4406182477422	22.05342487618131
36	24.144108517344147	27.074305507579204	28.632789142548127	20.14879683252852
37	23.063509041920874	29.562352447905628	28.651609106956954	18.722529403216544
38	23.549731160381832	28.43005762720747	26.28744041337218	21.732770799038516
39	23.154035453001306	27.821148398992772	28.109641777462258	20.91517437054366
40	24.96480190200754	25.638747209761604	29.508513056052525	19.887937832178327
41	21.00022155147975	26.80915841407257	30.221527657009723	21.96909237743796
42	23.566407078212436	26.303401649010045	28.604916537031254	21.525274735746258
43	24.06906688710642	26.156891799498293	28.490090931397656	21.28395038199763
44	22.729276003116013	27.20866575981437	28.1594313035565	21.902626933513115
45	22.21232255036723	27.20556880364583	27.401391724456666	23.18071692153028
46	23.045641987102368	27.382333532650254	27.901431031977264	21.67059344827011
47	21.935740541776745	27.120998077504904	30.05095684034238	20.89230454037597
48	23.09543151319661	24.50573770687072	30.342070720185244	22.056760059747432
49	21.902626933513115	25.417672184807287	30.328253531125597	22.351447350554
50	21.127673209185097	25.779063146936277	30.165305991180823	22.927957652697806
51	21.25131322852916	25.29689089423418	28.741897290639805	24.70989858659685
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	154.0
1	514.0
2	874.0
3	3439.5
4	6005.0
5	4072.5
6	2140.0
7	1996.0
8	1852.0
9	1800.0
10	1748.0
11	1716.5
12	1685.0
13	1682.0
14	1679.0
15	1510.0
16	1341.0
17	1384.0
18	1427.0
19	1555.0
20	1683.0
21	1952.0
22	2221.0
23	2548.0
24	2875.0
25	3234.0
26	3908.5
27	4224.0
28	5398.0
29	6572.0
30	7806.0
31	9040.0
32	9826.5
33	10613.0
34	11213.0
35	11813.0
36	13017.5
37	14222.0
38	14739.0
39	15256.0
40	17698.0
41	20140.0
42	22484.0
43	24828.0
44	27059.5
45	29291.0
46	32356.0
47	35421.0
48	35326.5
49	35232.0
50	33581.5
51	31931.0
52	28414.5
53	24898.0
54	22429.5
55	19961.0
56	19532.5
57	19104.0
58	19001.5
59	18899.0
60	18306.0
61	17713.0
62	16130.5
63	14548.0
64	12154.5
65	9761.0
66	8116.0
67	6471.0
68	5092.5
69	3714.0
70	3238.0
71	2762.0
72	2308.5
73	1855.0
74	1519.5
75	800.0
76	416.0
77	352.5
78	289.0
79	220.0
80	151.0
81	110.5
82	70.0
83	58.5
84	47.0
85	32.0
86	17.0
87	17.0
88	17.0
89	18.5
90	20.0
91	12.0
92	4.0
93	5.0
94	6.0
95	3.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	419767.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.01176669467851
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.96127771984519	24.415924445278534
2	9.33595471847202	6.163927180765627
3	3.63461667743996	3.5995535334070663
4	2.137503563757915	2.8225107582328066
5	1.4079898164017413	2.324011566376876
6	0.9899926313618597	1.9608843465581143
7	0.7840110746473642	1.811711347761208
8	0.6433983894394019	1.6991774019124353
9	0.5298118908133158	1.5741023878436102
>10	6.373078320607128	46.57318295060228
>50	0.16189215777130864	3.3103929786101696
>100	0.036136642359667105	2.116027050615754
>500	0.0036136642359667105	0.8100024621536233
>1k	7.227328471933421E-4	0.8185915898819093
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3431	0.8173582010972754	No Hit
GCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTCTGC	820	0.1953464660156706	Illumina Single End Adapter 1 (95% over 23bp)
GAATGATACCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCG	758	0.18057636736570526	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGC	738	0.17581181941410354	No Hit
CCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTCTGC	565	0.13459847963274865	Illumina Single End Adapter 1 (95% over 23bp)
GAATCTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTC	514	0.12244888235616426	No Hit
CTGTCTCTTATACACATCTGACGCTTGAGAGGTCGTATGCCGTCTTCTGCT	467	0.11125219466990022	Illumina Single End Adapter 1 (95% over 24bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.1305486138738872	0.0
2	0.0	0.0	0.0	0.6303496939969078	0.0
3	0.0	0.0	0.0	0.8321282997472408	0.0
4	0.0	0.0	0.0	1.101087031615158	0.0
5	0.0	0.0	0.0	1.9170158683269527	0.0
6	0.0	0.0	0.0	2.2991326140454107	0.0
7	0.0	0.0	0.0	2.7069779187025182	0.0
8	0.0	0.0	0.0	3.434286163514521	0.0
9	0.0	0.0	0.0	3.6999097118163173	0.0
10	0.0	0.0	0.0	4.5082152718055495	0.0
11	0.0	0.0	0.0	5.488282785450023	0.0
12	0.0	0.0	0.0	6.359718605797978	0.0
13	0.0	0.0	0.0	6.685375458289956	0.0
14	0.0	0.0	0.0	6.832599989994449	0.0
15	0.0	0.0	0.0	7.135148784921158	0.0
16	0.0	0.0	0.0	7.698794807595642	0.0
17	2.382273975800861E-4	0.0	0.0	8.337720687905433	0.0
18	2.382273975800861E-4	0.0	0.0	9.096713176595587	0.0
19	2.382273975800861E-4	0.0	0.0	9.554824462142093	0.0
20	2.382273975800861E-4	0.0	0.0	9.969578361329024	0.0
21	2.382273975800861E-4	0.0	0.0	10.48486422229475	0.0
22	2.382273975800861E-4	0.0	0.0	11.044936834005531	0.0
23	2.382273975800861E-4	0.0	0.0	11.59023934706635	0.0
24	2.382273975800861E-4	0.0	0.0	12.018572207915344	0.0
25	2.382273975800861E-4	0.0	0.0	12.405453501585404	0.0
26	2.382273975800861E-4	0.0	0.0	12.756838913016031	0.0
27	2.382273975800861E-4	0.0	0.0	13.113703554591	0.0
28	2.382273975800861E-4	0.0	0.0	13.464850738624046	0.0
29	2.382273975800861E-4	0.0	0.0	13.851493804896526	0.0
30	2.382273975800861E-4	0.0	0.0	14.301028904130149	0.0
31	2.382273975800861E-4	0.0	0.0	14.704586115630814	0.0
32	2.382273975800861E-4	0.0	0.0	15.144353891563654	0.0
33	2.382273975800861E-4	0.0	0.0	15.530758730438553	0.0
34	2.382273975800861E-4	0.0	0.0	15.903822835048967	0.0
35	2.382273975800861E-4	0.0	0.0	16.332632150693122	0.0
36	2.382273975800861E-4	0.0	0.0	16.764300195108238	0.0
37	2.382273975800861E-4	0.0	0.0	17.196206466920934	0.0
38	2.382273975800861E-4	0.0	0.0	17.583564215386154	0.0
39	2.382273975800861E-4	0.0	0.0	18.008085437873866	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTCACG	20	7.028163E-4	45.000004	1
TCCGCGA	20	7.028163E-4	45.000004	33
ATAATCC	20	7.028163E-4	45.000004	39
CGCGAAT	20	7.028163E-4	45.000004	35
ACGATAT	20	7.028163E-4	45.000004	12
CTAACGA	30	2.1621963E-6	45.000004	13
TATTCGG	20	7.028163E-4	45.000004	2
CGTCGAC	20	7.028163E-4	45.000004	17
ACCCGTA	20	7.028163E-4	45.000004	12
ACCCGAT	20	7.028163E-4	45.000004	41
CGGTAGT	30	2.1621963E-6	45.000004	12
TTACCGG	20	7.028163E-4	45.000004	2
TGCACGC	20	7.028163E-4	45.000004	43
CCCGATT	20	7.028163E-4	45.000004	42
TCGCAGC	25	3.8865604E-5	45.0	14
GCACGAT	25	3.8865604E-5	45.0	14
CACGATC	25	3.8865604E-5	45.0	15
TTGCGAT	35	1.2094642E-7	45.0	19
ACCCGTG	25	3.8865604E-5	45.0	35
CGATCCC	25	3.8865604E-5	45.0	43
>>END_MODULE