##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936739.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	442742
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.156852071861266	31.0	31.0	33.0	28.0	34.0
2	31.211563393579105	31.0	31.0	34.0	28.0	34.0
3	30.948292685130394	31.0	31.0	34.0	27.0	34.0
4	34.90912540486333	35.0	35.0	37.0	32.0	37.0
5	35.17940922704419	35.0	35.0	37.0	33.0	37.0
6	35.16938081320498	36.0	35.0	37.0	32.0	37.0
7	31.080010480144193	35.0	35.0	37.0	0.0	37.0
8	33.09829878349016	36.0	35.0	37.0	17.0	37.0
9	36.31112928070976	39.0	35.0	39.0	32.0	39.0
10	36.14738154500815	37.0	35.0	39.0	32.0	39.0
11	36.398202564924944	38.0	35.0	39.0	32.0	39.0
12	36.42295964692756	38.0	35.0	39.0	32.0	39.0
13	36.25083231317562	38.0	35.0	39.0	32.0	39.0
14	37.28975114174847	39.0	36.0	40.0	32.0	41.0
15	37.49639745043389	39.0	36.0	41.0	32.0	41.0
16	37.435885910982016	39.0	36.0	40.0	32.0	41.0
17	37.36848096634157	39.0	36.0	40.0	32.0	41.0
18	37.24740593844722	39.0	36.0	40.0	32.0	41.0
19	37.02159496953079	39.0	35.0	40.0	32.0	41.0
20	36.98555592195906	39.0	35.0	40.0	32.0	41.0
21	37.01064728442298	39.0	35.0	40.0	32.0	41.0
22	37.09996566849316	39.0	35.0	40.0	32.0	41.0
23	37.16491997596795	39.0	35.0	40.0	32.0	41.0
24	37.09299772779632	39.0	35.0	40.0	32.0	41.0
25	36.7677744600693	38.0	35.0	40.0	31.0	41.0
26	36.751916466023104	38.0	35.0	40.0	31.0	41.0
27	36.78710174322743	39.0	35.0	40.0	31.0	41.0
28	36.769750780364184	39.0	35.0	40.0	31.0	41.0
29	36.762676683034364	39.0	35.0	40.0	31.0	41.0
30	36.52925179901613	38.0	35.0	40.0	31.0	41.0
31	36.486725903573635	38.0	35.0	40.0	31.0	41.0
32	36.25675223945323	38.0	35.0	40.0	30.0	41.0
33	36.11795357115431	38.0	35.0	40.0	30.0	41.0
34	36.00149974477235	38.0	35.0	40.0	30.0	41.0
35	35.79820527530706	38.0	35.0	40.0	29.0	41.0
36	35.74321162211852	38.0	35.0	40.0	29.0	41.0
37	35.66810693360919	38.0	35.0	40.0	28.0	41.0
38	35.491559418352	38.0	35.0	40.0	27.0	41.0
39	35.45712853083737	38.0	35.0	40.0	27.0	41.0
40	35.26863952369551	38.0	34.0	40.0	26.0	41.0
41	35.05728166742708	38.0	34.0	40.0	25.0	41.0
42	35.04060152413821	38.0	34.0	40.0	25.0	41.0
43	34.9917762489215	38.0	34.0	40.0	25.0	41.0
44	34.831996964372024	38.0	34.0	40.0	24.0	41.0
45	34.762367247742475	37.0	34.0	40.0	24.0	41.0
46	34.62857149310434	37.0	34.0	40.0	24.0	41.0
47	34.54807992013407	37.0	33.0	40.0	23.0	41.0
48	34.45007024406991	37.0	33.0	40.0	23.0	41.0
49	34.329094596853245	37.0	33.0	40.0	23.0	41.0
50	34.16652361872151	37.0	33.0	40.0	23.0	41.0
51	32.3908280669103	35.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	9.0
9	38.0
10	45.0
11	29.0
12	19.0
13	15.0
14	22.0
15	30.0
16	44.0
17	120.0
18	194.0
19	399.0
20	643.0
21	1059.0
22	1617.0
23	2394.0
24	3476.0
25	4981.0
26	6415.0
27	7339.0
28	7976.0
29	8924.0
30	10700.0
31	13350.0
32	17604.0
33	24573.0
34	38329.0
35	40500.0
36	43568.0
37	64100.0
38	82195.0
39	62031.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.47710856435577	20.684507004079126	22.877432003288597	13.960952428276514
2	34.427499536976384	23.900149522746883	24.527377118050694	17.144973822226035
3	31.440658442162707	23.573548477442845	29.305329062975726	15.680464017418721
4	28.050648007191548	24.76114757578906	29.35569699734834	17.83250741967105
5	25.869693862339695	27.910385732548527	27.817781010159415	18.402139394952364
6	25.562291357043154	33.77994407578229	26.90889953968677	13.748865027487792
7	65.83585925889118	18.217381680527257	12.144318813214017	3.802440247367541
8	76.16986868198636	6.826774961489987	12.19671953417566	4.806636822348004
9	70.35745422842197	7.532151907883146	14.358249273843457	7.752144589851426
10	41.69380813204982	23.06264144806682	20.91511534934567	14.328435070537695
11	30.498348925559355	23.836003812604183	27.474917672143146	18.19072958969332
12	28.46014157229267	21.60106789055477	31.48537974712135	18.453410790031214
13	23.47574885599288	25.22959195197203	33.660235532206116	17.634423659828975
14	19.53711190716038	27.85730741605721	32.149423366204246	20.456157310578167
15	18.479837015688595	23.908732399456117	38.534631907521764	19.076798677333525
16	21.15972733555886	24.201227803099773	33.681918589155764	20.957126272185608
17	21.895821945964013	22.85077991245466	31.555849682207697	23.69754845937363
18	23.84142457684159	22.81848119220675	32.940177349336636	20.399916881615027
19	23.718102190440483	25.133147521581417	29.596243410383472	21.552506877594627
20	25.026539158245658	24.999887067411724	30.977860695393705	18.995713078948913
21	24.20416405039504	26.1981018290562	30.855667634875388	18.742066485673373
22	22.99239737815703	24.27937715418912	30.622123042313582	22.106102425340264
23	21.431443142959104	25.119369745811333	31.76884054370265	21.68034656752691
24	22.454612392770507	23.404827190553416	31.96624670801505	22.174313708661025
25	20.989199127256956	25.98578856309092	30.770290598136164	22.254721711515963
26	20.35677663289229	25.873533570341188	30.936301502906883	22.83338829385963
27	20.750685500810857	25.914641032474893	31.61592981917234	21.71874364754191
28	18.028784258100654	25.783413364894226	33.57711714723247	22.610685229772645
29	19.853774884695827	24.27960301936568	33.34876745373152	22.517854642206974
30	21.81902778593402	24.596717727254248	32.486414209629984	21.097840277181746
31	23.506692385181438	24.170961869440895	29.379638706063577	22.942707039314094
32	22.66286008555773	25.134728577817327	30.375478269511362	21.82693306711358
33	23.232266195662486	24.321162211852503	30.179879026611435	22.266692565873576
34	22.68183276038867	23.892921837097	31.481314173943293	21.943931228571042
35	21.2132573824033	24.44832430625511	32.28178035966771	22.05663795167389
36	23.141242529509285	25.20610197360991	31.664264966955923	19.988390529924878
37	21.052667241869983	25.540834165270066	31.78487697123833	21.62162162162162
38	21.437541502726194	26.19200346928911	28.050873872368104	24.31958115561659
39	23.324870918051595	24.30264126737468	28.783580505124878	23.588907309448846
40	23.984848963956434	23.22932994836722	31.288425313162065	21.497395774514278
41	21.26723915960085	23.92499469216835	30.772549249901747	24.035216898329047
42	21.45154514367284	22.833614159036188	32.554173762597635	23.160666934693346
43	21.77408061579882	23.01159591816453	31.398195788969645	23.816127677067005
44	21.001169981614577	24.035668628682167	30.21850197180299	24.744659417900266
45	21.402758265536136	23.681060301484838	28.974210714140515	25.94197071883851
46	23.515726992243792	24.024375369854226	29.683201503358614	22.776696134543368
47	20.309344945814946	24.7993187906275	32.995062587240426	21.896273676317133
48	21.220710933229736	23.2480767580216	31.484250421238553	24.046961887510108
49	21.641949487511912	22.143821909825586	33.84883295463272	22.36539564802978
50	21.195639898631708	23.17444471046343	32.424978881605995	23.204936509298868
51	20.42589137691929	22.567319115873353	30.3379846502026	26.66880485700476
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	194.0
1	373.0
2	552.0
3	3806.0
4	7060.0
5	4643.5
6	2227.0
7	2101.0
8	1975.0
9	1945.0
10	1915.0
11	1897.0
12	1879.0
13	1809.0
14	1739.0
15	1762.5
16	1786.0
17	1725.5
18	1665.0
19	1623.5
20	1582.0
21	1565.0
22	1548.0
23	2095.5
24	2643.0
25	2913.5
26	3208.0
27	3232.0
28	4393.0
29	5554.0
30	6383.5
31	7213.0
32	8368.5
33	9524.0
34	11720.0
35	13916.0
36	14116.5
37	14317.0
38	15411.5
39	16506.0
40	18480.0
41	20454.0
42	23048.5
43	25643.0
44	28622.5
45	31602.0
46	37501.5
47	43401.0
48	41196.5
49	38992.0
50	37209.0
51	35426.0
52	31522.5
53	27619.0
54	25287.5
55	22956.0
56	21747.5
57	20539.0
58	19381.5
59	18224.0
60	17140.5
61	16057.0
62	15116.5
63	14176.0
64	11951.5
65	9727.0
66	8361.5
67	6996.0
68	5614.0
69	4232.0
70	3527.5
71	2823.0
72	2173.5
73	1524.0
74	1368.0
75	817.0
76	422.0
77	345.5
78	269.0
79	205.5
80	142.0
81	97.5
82	53.0
83	34.5
84	16.0
85	12.5
86	9.0
87	9.0
88	9.0
89	6.5
90	4.0
91	2.5
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	442742.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.854732479922674
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.14955562238931	25.44168101581059
2	9.435494576646866	6.388722894162818
3	3.67509169459891	3.7325773847949537
4	2.053883800254863	2.7813474640990132
5	1.2757341405417413	2.1594819021772365
6	0.9438773323219317	1.9172828747773243
7	0.7030649354775695	1.6661452714625047
8	0.5593314293008786	1.514881272527522
9	0.4082683509099916	1.2439634220069264
>10	5.515620359233668	43.262428653367365
>50	0.24540146443650365	5.066977522915041
>100	0.029341479443495005	1.7530333481302482
>500	0.0020005554166019324	0.4366215705452543
>1k	0.0033342590276698872	2.6348554032231974
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4137	0.9344042354237908	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGC	2195	0.4957740625465847	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCG	2168	0.48967570277949685	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTC	1712	0.3866811822686802	No Hit
GCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTCTGC	1459	0.32953729259930165	TruSeq Adapter, Index 21 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTCTGC	711	0.16059014053331286	TruSeq Adapter, Index 21 (95% over 23bp)
GAATGCTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTT	676	0.15268485935375456	No Hit
CTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCTTCTGCT	547	0.12354825157766826	Illumina Paired End PCR Primer 2 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCTTCCGGTTTCGTATGCCGTCT	444	0.10028413839211098	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.15449178076622502	0.0
2	0.0	0.0	0.0	0.9312421229519675	0.0
3	0.0	0.0	0.0	1.185566311757186	0.0
4	0.0	0.0	0.0	1.5975443938004525	0.0
5	0.0	0.0	0.0	2.9448301719737455	0.0
6	0.0	0.0	0.0	3.588997655519467	0.0
7	0.0	0.0	0.0	4.171729811041193	0.0
8	0.0	0.0	0.0	5.077900899395133	0.0
9	0.0	0.0	0.0	5.371977359274702	0.0
10	0.0	0.0	0.0	6.623722167763619	0.0
11	0.0	0.0	0.0	7.703131846538165	0.0
12	0.0	0.0	0.0	9.08384567084216	0.0
13	0.0	0.0	0.0	9.547546878317394	0.0
14	0.0	0.0	0.0	9.772282728993408	0.0
15	0.0	0.0	0.0	10.197360991277087	0.0
16	0.0	0.0	0.0	10.869309891539542	0.0
17	0.0	0.0	0.0	11.616471895596082	0.0
18	0.0	0.0	0.0	12.455561026512054	0.0
19	0.0	0.0	0.0	12.970533629066137	0.0
20	0.0	0.0	0.0	13.455917893491018	0.0
21	0.0	0.0	0.0	14.02396881253642	0.0
22	0.0	0.0	0.0	14.582759259342913	0.0
23	0.0	0.0	0.0	15.122802896495024	0.0
24	0.0	0.0	0.0	15.602314666329375	0.0
25	0.0	0.0	0.0	16.01858418672726	0.0
26	0.0	0.0	0.0	16.430562268770526	0.0
27	0.0	0.0	0.0	16.83147295716241	0.0
28	0.0	0.0	0.0	17.230124993788706	0.0
29	0.0	0.0	0.0	17.673950065726768	0.0
30	0.0	0.0	0.0	18.179210465688822	0.0
31	0.0	0.0	0.0	18.609483627033352	0.0
32	0.0	0.0	0.0	19.039079192848206	0.0
33	0.0	0.0	0.0	19.44812102759621	0.0
34	0.0	0.0	0.0	19.888106391532766	0.0
35	0.0	0.0	0.0	20.356550767715735	0.0
36	0.0	0.0	0.0	20.765818467640297	0.0
37	0.0	0.0	0.0	21.190896729923974	0.0
38	0.0	0.0	0.0	21.649403038338356	0.0
39	0.0	0.0	0.0	22.173636113131348	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGAATT	30	2.1624674E-6	45.000004	34
TACGCGG	45	3.8380676E-10	45.000004	2
ACTAACG	30	2.1624674E-6	45.000004	1
CCAACTC	30	2.1624674E-6	45.000004	42
TGCACGG	30	2.1624674E-6	45.000004	2
TCACGAC	20	7.0285815E-4	45.0	25
GTCGATA	20	7.0285815E-4	45.0	25
GATCGGC	20	7.0285815E-4	45.0	12
CGAAAGG	25	3.886907E-5	45.0	2
TAGGTCG	35	1.2096643E-7	45.0	23
CGTGACG	25	3.886907E-5	45.0	19
CGTTGGC	20	7.0285815E-4	45.0	40
GAATCGT	20	7.0285815E-4	45.0	28
GCACGGT	20	7.0285815E-4	45.0	3
CGTCTGG	25	3.886907E-5	45.0	2
TAACGGT	20	7.0285815E-4	45.0	17
ACGGGTC	20	7.0285815E-4	45.0	5
AACGGTC	25	3.886907E-5	45.0	6
CGGATGC	20	7.0285815E-4	45.0	38
TGCGATG	20	7.0285815E-4	45.0	12
>>END_MODULE