FastQCFastQC Report
Sat 14 Jan 2017
SRR2936738.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2936738.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences928185
Sequences flagged as poor quality0
Sequence length51
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT110631.191896012109655No Hit
GAATCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTC19020.20491604583138062RNA PCR Primer, Index 27 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCG14440.15557243437461282No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGC13060.14070470865183127No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTT11760.12669888007239935No Hit
GCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGC10510.113231737207561Illumina PCR Primer Index 6 (95% over 22bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGGTA207.0325856E-445.027
CGATCGG207.0325856E-445.02
CGTTTTT52000.043.1826931
CGTTTTA4900.040.4081651
ACAACGA2600.038.0769213
GAAATCG301.1397527E-437.49999623
AGACACG1400.036.96428724
GCGAGAC1350.036.66666821
CACAACG2700.036.66666812
GGGCGAT12400.036.2903257
TCGTTAC250.002106899936.037
CTATCGT250.002106899936.034
TCGTAGC250.002106899936.014
TATAGCG504.8772563E-836.01
ATACGCC250.002106899936.028
TCGACGG250.002106899936.02
AATACCG250.002106899936.024
AGCGACT2650.035.66037419
TCGTCCC1200.035.62538
GACACGA1400.035.35714325
Produced by FastQC (version 0.11.5)