##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936738.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	928185
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.098369398341926	31.0	31.0	33.0	28.0	34.0
2	31.168374839067642	31.0	31.0	34.0	28.0	34.0
3	30.860037600262878	31.0	31.0	34.0	27.0	34.0
4	34.81205901840689	35.0	35.0	37.0	32.0	37.0
5	35.11430264440817	35.0	35.0	37.0	33.0	37.0
6	35.1055145256603	36.0	35.0	37.0	32.0	37.0
7	31.012574001950043	35.0	35.0	37.0	0.0	37.0
8	33.08933671627962	36.0	35.0	37.0	17.0	37.0
9	36.45573026928899	39.0	35.0	39.0	32.0	39.0
10	36.170135263982935	37.0	35.0	39.0	32.0	39.0
11	36.37331458707047	38.0	35.0	39.0	32.0	39.0
12	36.48618971433496	38.0	35.0	39.0	32.0	39.0
13	36.52593071424339	39.0	35.0	39.0	32.0	39.0
14	37.57815198478752	39.0	36.0	41.0	33.0	41.0
15	37.75941434089109	40.0	37.0	41.0	33.0	41.0
16	37.65869735020497	39.0	36.0	41.0	33.0	41.0
17	37.55460064534549	39.0	36.0	41.0	32.0	41.0
18	37.418475842639126	39.0	36.0	40.0	32.0	41.0
19	37.18626459164929	39.0	36.0	40.0	32.0	41.0
20	37.13710413333549	39.0	35.0	40.0	32.0	41.0
21	37.127714841330125	39.0	35.0	40.0	32.0	41.0
22	37.2101768505201	39.0	35.0	40.0	32.0	41.0
23	37.259195095805254	39.0	35.0	40.0	32.0	41.0
24	37.191127846280644	39.0	35.0	40.0	32.0	41.0
25	36.85009884882863	39.0	35.0	40.0	31.0	41.0
26	36.856124587232074	39.0	35.0	40.0	31.0	41.0
27	36.89283601868162	39.0	35.0	40.0	31.0	41.0
28	36.902717669430125	39.0	35.0	40.0	31.0	41.0
29	36.88153116027516	39.0	35.0	40.0	31.0	41.0
30	36.61497115337998	39.0	35.0	40.0	31.0	41.0
31	36.549865597914206	39.0	35.0	40.0	30.0	41.0
32	36.36043245689167	39.0	35.0	40.0	30.0	41.0
33	36.13314587070465	39.0	35.0	40.0	30.0	41.0
34	35.947232502141276	39.0	35.0	40.0	29.0	41.0
35	35.80157834914376	39.0	35.0	40.0	27.0	41.0
36	35.7330219729903	39.0	35.0	40.0	27.0	41.0
37	35.66451300117972	38.0	35.0	40.0	27.0	41.0
38	35.50925731400529	38.0	35.0	40.0	26.0	41.0
39	35.4329481730474	38.0	35.0	40.0	26.0	41.0
40	35.32551700361458	38.0	35.0	40.0	25.0	41.0
41	35.15729407391845	38.0	34.0	40.0	24.0	41.0
42	35.175647096214654	38.0	34.0	40.0	24.0	41.0
43	35.14751046397001	38.0	34.0	40.0	24.0	41.0
44	34.93575957379186	38.0	34.0	40.0	24.0	41.0
45	34.85300236483029	38.0	34.0	40.0	23.0	41.0
46	34.76310433803606	38.0	34.0	40.0	23.0	41.0
47	34.72945587355969	38.0	34.0	40.0	23.0	41.0
48	34.64684410974105	38.0	34.0	40.0	23.0	41.0
49	34.5426709115101	38.0	34.0	40.0	23.0	41.0
50	34.381413188103664	37.0	33.0	40.0	23.0	41.0
51	32.82977208207416	36.0	31.0	39.0	19.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	7.0
8	47.0
9	85.0
10	99.0
11	65.0
12	42.0
13	36.0
14	45.0
15	81.0
16	150.0
17	289.0
18	630.0
19	957.0
20	1668.0
21	2476.0
22	3774.0
23	5357.0
24	7861.0
25	11585.0
26	14818.0
27	16356.0
28	17156.0
29	18581.0
30	21656.0
31	26136.0
32	33495.0
33	44900.0
34	66516.0
35	75435.0
36	91730.0
37	137286.0
38	182283.0
39	146580.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.653501187802	21.687486869535707	24.009976459434274	15.64903548322802
2	36.05692830631824	23.017501898867142	24.57581193404332	16.34975786077129
3	31.17481967495704	22.896513087369435	30.368622634496354	15.560044603177166
4	26.740897558137654	25.222773477270156	29.80774306846157	18.22858589613062
5	24.78633031130647	28.968578462267757	28.4850541648486	17.760037061577165
6	25.733986220419418	33.29120811045212	27.413608278522062	13.5611973906064
7	68.20558401611748	17.013526398293443	11.465817697980468	3.3150718876086125
8	80.74026190899444	4.4925311225671605	11.363359675064777	3.403847293373627
9	74.42826591681614	7.102571146915755	13.284420670448242	5.184742265819853
10	37.825648981614655	30.53604615459203	19.414879576808502	12.223425286984813
11	27.15374628980214	25.19917904297096	30.702823251830186	16.944251415396717
12	24.5526484483158	23.293093510453197	33.385585847648905	18.7686721935821
13	22.363429704207675	23.30440591045966	35.305461734460266	19.0267026508724
14	19.190570845251756	25.98070427770326	34.67853929981631	20.150185577228676
15	19.050081610885762	25.843554894767745	36.251286112143596	18.8550773822029
16	23.356658424775233	25.800352300457348	32.76264968729294	18.08033958747448
17	23.514493339151137	25.61730689463846	31.30873694360499	19.559462822605408
18	23.356227476203557	25.47423196884242	31.995884441140504	19.173656113813518
19	22.923878321670788	26.89485393536849	30.47043423455453	19.71083350840619
20	24.039496436594	26.79573576388328	31.28880557216503	17.875962227357693
21	24.000603328000345	26.238519260707726	32.12204463549831	17.63883277579362
22	23.050038516028593	23.579566573474036	32.51905600715374	20.85133890334362
23	21.635018880934297	25.913799511950742	32.936645173106655	19.514536434008306
24	21.512629486578646	25.149619957228353	32.96217887597839	20.375571680214613
25	22.02524281258585	26.97641095255795	30.846113651912066	20.152232582944134
26	21.47578338370045	26.890975398223414	30.79870930902783	20.834531909048305
27	21.12908525778805	25.999127329142357	31.853348201059056	21.018439212010538
28	20.08575876576329	26.549340918028197	31.843867332482212	21.521032983726304
29	21.421914812241095	26.19897972925656	31.15725852066129	21.221846937841054
30	21.698583795256337	25.320167854468668	31.881575332503758	21.09967301777124
31	22.528913955730808	25.868765386210725	30.0319440628754	21.57037659518307
32	22.780695658731826	25.95312356911607	29.965362508551635	21.30081826360047
33	21.623167795213238	25.515387557437364	30.41376449737929	22.447680149970104
34	21.410171463662955	26.13304459779031	31.609754520919857	20.847029417626874
35	20.56982174889704	26.7705252724403	31.15413414351665	21.505518835146013
36	21.45951507511972	27.445390735683077	30.387799845935888	20.707294343261314
37	20.961015314834867	27.54709459859834	29.918604588524918	21.573285498041876
38	20.784326400448187	27.302208072744115	30.50394048600225	21.409525040805445
39	21.660337109520192	26.14457247208261	30.29062094302321	21.904469475373983
40	22.605084115774336	26.052672689172958	31.16404596066517	20.17819723438754
41	20.426208137386404	26.50796985514741	30.771128600440644	22.29469340702554
42	21.12402161207087	26.23388656356222	30.762940577578824	21.879151246788087
43	21.11658774920948	25.57959889461691	30.74893474899939	22.554878607174217
44	21.431395680817943	25.711792368978166	29.828428599901958	23.028383350301933
45	21.0764017949008	25.357337168775622	29.71562781126607	23.850633225057503
46	21.556693978032396	26.490731912280417	29.36752910249573	22.585045007191454
47	20.897665874798665	26.222035477841168	31.138727732079275	21.741570915280896
48	20.669801817525602	26.05224174060128	30.645722566083272	22.632233875789847
49	21.525019258014296	25.139384928651076	31.258746909290714	22.076848904043914
50	20.153094480087482	25.866179694780673	31.039825034879897	22.940900790251945
51	20.140381497223075	25.32189164875537	30.30527319446016	24.2324536595614
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	311.0
1	737.0
2	1163.0
3	9878.0
4	18593.0
5	12386.5
6	6180.0
7	5755.5
8	5331.0
9	5323.5
10	5316.0
11	5117.0
12	4918.0
13	4821.0
14	4724.0
15	4482.0
16	4240.0
17	4164.5
18	4089.0
19	4097.5
20	4106.0
21	4203.0
22	4300.0
23	5080.5
24	5861.0
25	7077.0
26	9640.5
27	10988.0
28	12982.0
29	14976.0
30	17837.0
31	20698.0
32	23101.5
33	25505.0
34	28391.0
35	31277.0
36	34286.5
37	37296.0
38	39866.0
39	42436.0
40	44594.5
41	46753.0
42	49467.0
43	52181.0
44	55237.5
45	58294.0
46	65996.0
47	73698.0
48	74140.0
49	74582.0
50	71501.0
51	68420.0
52	63046.0
53	57672.0
54	52435.5
55	47199.0
56	43930.5
57	40662.0
58	39010.5
59	37359.0
60	34168.5
61	30978.0
62	28099.0
63	25220.0
64	21744.0
65	18268.0
66	15446.0
67	12624.0
68	10950.0
69	9276.0
70	7639.0
71	6002.0
72	4875.0
73	3748.0
74	2998.5
75	1740.0
76	1231.0
77	928.5
78	626.0
79	424.5
80	223.0
81	192.5
82	162.0
83	120.0
84	78.0
85	53.0
86	28.0
87	25.5
88	23.0
89	19.5
90	16.0
91	11.0
92	6.0
93	4.0
94	2.0
95	2.5
96	3.0
97	2.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	928185.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.38844613169605
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.86678489021419	25.786422918772285
2	9.28595504348019	6.572310396750999
3	4.207349402754111	4.466746730897621
4	2.4575449234871782	3.4787478456419634
5	1.6540175124157501	2.9266554819503336
6	1.1637338880484884	2.470964040529988
7	0.848897666122336	2.1028818530224953
8	0.6847419115028037	1.938556179946524
9	0.5890905488759528	1.876229924002912
>10	6.138863709309231	42.93814225300792
>50	0.0800941351193972	1.7521671908831769
>100	0.01875793800319217	1.2278456806287603
>500	0.002381960381224467	0.571828585648434
>1k	0.0014887252382652918	0.7248219717478491
>5k	0.0	0.0
>10k+	2.977450476530584E-4	1.1656789465687534
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	11063	1.191896012109655	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTC	1902	0.20491604583138062	RNA PCR Primer, Index 27 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCG	1444	0.15557243437461282	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGC	1306	0.14070470865183127	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTT	1176	0.12669888007239935	No Hit
GCTGTCTCTTATACACATCTGACGCGCCCTATTTCGTATGCCGTCTTCTGC	1051	0.113231737207561	Illumina PCR Primer Index 6 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.08123380576070503	0.0
2	0.0	0.0	0.0	0.3605962173489121	0.0
3	0.0	0.0	0.0	0.5450422060257384	0.0
4	0.0	0.0	0.0	0.8318384804753363	0.0
5	0.0	0.0	0.0	1.5950484009114563	0.0
6	0.0	0.0	0.0	2.2236946298421114	0.0
7	0.0	0.0	0.0	2.7206860701261064	0.0
8	0.0	0.0	0.0	3.4636414076935096	0.0
9	0.0	0.0	0.0	3.832533385047162	0.0
10	0.0	0.0	0.0	4.559974574034271	0.0
11	0.0	0.0	0.0	5.1805405172460235	0.0
12	0.0	0.0	0.0	5.811126014749215	0.0
13	0.0	0.0	0.0	6.063230929178989	0.0
14	0.0	0.0	0.0	6.20231958068704	0.0
15	0.0	0.0	0.0	6.3860114093634355	0.0
16	0.0	0.0	0.0	6.6826117638186355	0.0
17	0.003555325716317329	0.0	0.0	7.034481272591132	0.0
18	0.003555325716317329	0.0	0.0	7.427290895672738	0.0
19	0.003555325716317329	0.0	0.0	7.68704514724974	0.0
20	0.003555325716317329	0.0	0.0	7.939904221679945	0.0
21	0.003555325716317329	0.0	0.0	8.248463398999123	0.0
22	0.003555325716317329	0.0	0.0	8.582987227761707	0.0
23	0.003555325716317329	0.0	0.0	8.92279017652731	0.0
24	0.003555325716317329	0.0	0.0	9.192348508109914	0.0
25	0.003663062859236036	0.0	0.0	9.435618976820354	0.0
26	0.003663062859236036	0.0	0.0	9.679428131245388	0.0
27	0.003663062859236036	0.0	0.0	9.927762245673007	0.0
28	0.003663062859236036	0.0	0.0	10.182560588675749	0.0
29	0.003663062859236036	0.0	0.0	10.469356863125347	0.0
30	0.003663062859236036	0.0	0.0	10.811314554749323	0.0
31	0.003663062859236036	0.0	0.0	11.113624977779214	0.0
32	0.003663062859236036	0.0	0.0	11.419059777953748	0.0
33	0.003663062859236036	0.0	0.0	11.729450486702543	0.0
34	0.003663062859236036	0.0	0.0	12.034131126876646	0.0
35	0.003663062859236036	0.0	0.0	12.363914521350809	0.0
36	0.003663062859236036	0.0	0.0	12.679368875816783	0.0
37	0.003663062859236036	0.0	0.0	12.989220898850983	0.0
38	0.003663062859236036	0.0	0.0	13.345184419054391	0.0
39	0.003663062859236036	0.0	0.0	13.794125093596643	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGTA	20	7.0325856E-4	45.0	27
CGATCGG	20	7.0325856E-4	45.0	2
CGTTTTT	5200	0.0	43.182693	1
CGTTTTA	490	0.0	40.408165	1
ACAACGA	260	0.0	38.07692	13
GAAATCG	30	1.1397527E-4	37.499996	23
AGACACG	140	0.0	36.964287	24
GCGAGAC	135	0.0	36.666668	21
CACAACG	270	0.0	36.666668	12
GGGCGAT	1240	0.0	36.290325	7
TCGTTAC	25	0.0021068999	36.0	37
CTATCGT	25	0.0021068999	36.0	34
TCGTAGC	25	0.0021068999	36.0	14
TATAGCG	50	4.8772563E-8	36.0	1
ATACGCC	25	0.0021068999	36.0	28
TCGACGG	25	0.0021068999	36.0	2
AATACCG	25	0.0021068999	36.0	24
AGCGACT	265	0.0	35.660374	19
TCGTCCC	120	0.0	35.625	38
GACACGA	140	0.0	35.357143	25
>>END_MODULE