##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936735.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	430275
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.260868049503223	31.0	31.0	33.0	30.0	34.0
2	31.302494916042065	31.0	31.0	34.0	28.0	34.0
3	31.057735169368428	31.0	31.0	34.0	28.0	34.0
4	35.03480797164604	35.0	35.0	37.0	32.0	37.0
5	35.28467375515659	35.0	35.0	37.0	33.0	37.0
6	35.259359711812216	37.0	35.0	37.0	33.0	37.0
7	30.868909418395212	35.0	35.0	37.0	0.0	37.0
8	33.0422450758236	36.0	35.0	37.0	17.0	37.0
9	36.38930916274476	39.0	35.0	39.0	32.0	39.0
10	36.2563058509093	37.0	35.0	39.0	32.0	39.0
11	36.4857591075475	38.0	35.0	39.0	32.0	39.0
12	36.47180524083435	38.0	35.0	39.0	32.0	39.0
13	36.35045494160711	38.0	35.0	39.0	32.0	39.0
14	37.31182615768985	39.0	36.0	40.0	32.0	41.0
15	37.53551798268549	39.0	36.0	41.0	33.0	41.0
16	37.52688861774447	39.0	36.0	41.0	33.0	41.0
17	37.455522630875606	39.0	36.0	40.0	33.0	41.0
18	37.40237987333682	39.0	36.0	40.0	32.0	41.0
19	37.20251931904015	39.0	36.0	40.0	32.0	41.0
20	37.20490384056708	39.0	35.0	40.0	32.0	41.0
21	37.19283016675382	39.0	35.0	40.0	32.0	41.0
22	37.305304746964154	39.0	35.0	40.0	32.0	41.0
23	37.35647202370577	39.0	35.0	40.0	33.0	41.0
24	37.29120213816745	39.0	35.0	41.0	32.0	41.0
25	36.96989367265121	39.0	35.0	40.0	32.0	41.0
26	36.99531927255825	39.0	35.0	40.0	32.0	41.0
27	37.03749462553019	39.0	35.0	40.0	32.0	41.0
28	37.054507001336354	39.0	35.0	40.0	32.0	41.0
29	37.041327058276686	39.0	35.0	40.0	32.0	41.0
30	36.837127418511415	39.0	35.0	40.0	31.0	41.0
31	36.78385451164953	39.0	35.0	40.0	31.0	41.0
32	36.59879147057114	39.0	35.0	40.0	31.0	41.0
33	36.52428563128232	39.0	35.0	40.0	31.0	41.0
34	36.42615768984952	39.0	35.0	40.0	30.0	41.0
35	36.29501597815351	39.0	35.0	40.0	30.0	41.0
36	36.26312706989716	38.0	35.0	40.0	30.0	41.0
37	36.17672418801929	38.0	35.0	40.0	30.0	41.0
38	36.03965835802684	38.0	35.0	40.0	30.0	41.0
39	36.02342920225437	38.0	35.0	40.0	30.0	41.0
40	35.86213468130847	38.0	35.0	40.0	29.0	41.0
41	35.69224798094242	38.0	35.0	40.0	29.0	41.0
42	35.70215327406891	38.0	35.0	40.0	29.0	41.0
43	35.684301899947705	38.0	35.0	40.0	29.0	41.0
44	35.50659229562489	38.0	34.0	40.0	28.0	41.0
45	35.432904537795594	38.0	34.0	40.0	28.0	41.0
46	35.35829179013422	38.0	34.0	40.0	28.0	41.0
47	35.257902504212424	38.0	34.0	40.0	27.0	41.0
48	35.18612747661379	37.0	34.0	40.0	27.0	41.0
49	35.10365928766487	37.0	34.0	40.0	27.0	41.0
50	34.953622683167744	37.0	34.0	40.0	27.0	41.0
51	33.245601069083726	35.0	31.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	21.0
9	43.0
10	39.0
11	34.0
12	26.0
13	12.0
14	22.0
15	26.0
16	69.0
17	96.0
18	213.0
19	333.0
20	532.0
21	837.0
22	1248.0
23	1797.0
24	2670.0
25	3542.0
26	4568.0
27	5479.0
28	6236.0
29	7448.0
30	9341.0
31	11958.0
32	15929.0
33	23308.0
34	36030.0
35	38761.0
36	44125.0
37	65235.0
38	84973.0
39	65321.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.01528092498983	20.420661205043285	25.16901981291035	11.395038057056533
2	34.501888327232585	23.261402591365986	25.170879088954738	17.06582999244669
3	33.29382371739004	23.709720527569576	27.07245366335483	15.92400209168555
4	29.07512637266864	25.343326942071933	26.050549067456856	19.530997617802566
5	25.641275928185465	29.734007320899426	25.28754866074022	19.337168090174888
6	26.55022950438673	34.17372610539771	26.011039451513568	13.265004938701994
7	68.61402591365987	18.492591947010634	8.927546336645168	3.9658358026843294
8	79.41711696008366	6.4619138922781945	9.76143164255418	4.359537505083958
9	72.69676369763523	8.129916913601766	12.394166521410725	6.779152867352274
10	43.13752832490849	25.256870606007787	18.364999128464355	13.24060194061937
11	33.58689210388705	24.945441868572424	24.061356109464878	17.40630991807565
12	29.69635698100052	23.16564987507989	29.084190343385046	18.05380280053454
13	25.495787577711926	26.326419150543256	29.542501888327234	18.63529138341758
14	20.63122421707048	28.120155714368718	30.15861948753704	21.090000581023762
15	19.47150078438208	25.570623438498636	35.39898901865086	19.55888675846842
16	23.371797106501656	25.476265179245832	31.238858869327757	19.913078844924758
17	23.850328278426588	24.810179536343036	28.856196618441693	22.483295566788684
18	26.36174539538667	23.841031898204637	29.804659810586255	19.992562895822438
19	25.994305967114055	26.179768752541975	26.97042589041892	20.855499389925047
20	26.984370460751844	25.765847423159606	28.431584451804078	18.81819766428447
21	24.85108360931962	27.184010226018245	28.979373656382545	18.985532508279586
22	24.533379815234444	23.51565859043635	29.39980245192028	22.551159142408924
23	22.252745337284292	26.612980070884902	29.662890012201498	21.47138457962931
24	22.984370460751844	24.32909185985707	30.88606123990471	21.800476439486374
25	21.724710940677475	27.192376968218003	29.010981349137175	22.071930741967346
26	21.126256463889373	27.271628609610133	28.721399105223405	22.88071582127709
27	22.712218929754226	26.628783917262215	29.57550403811516	21.0834931148684
28	19.46592295624891	26.98901865086282	31.20330021497879	22.341758177909476
29	21.11905176921736	26.8697925745163	30.908837371448495	21.10231828481785
30	24.061820928475974	25.469757713090463	29.826971123118934	20.641450235314622
31	26.129568299343443	26.117947824066007	27.27046656208239	20.482017314508163
32	24.962407762477483	27.325082795886352	28.46923479170298	19.24327464993318
33	25.91040613561095	25.853465806751498	26.77659636279124	21.45953169484632
34	22.568589855325083	25.794898611353208	30.508395793387947	21.128115739933765
35	21.447446400557784	27.339957004241473	29.527162860961013	21.68543373423973
36	24.951484515716693	28.248910580442736	26.98530009877404	19.814304805066527
37	23.349020974957877	28.710708267968162	28.037882749404453	19.902388007669515
38	23.446632967288362	28.677241299169136	25.979896577770027	21.89622915577247
39	23.849166230898845	26.21927836848527	27.656266341293357	22.275289059322525
40	26.24949160420661	24.431584451804078	28.642844692347918	20.67607925164139
41	21.75701586194875	25.21271279995352	28.630294579048282	24.399976759049444
42	22.993434431468245	25.121840683283946	29.11184707454535	22.772877810702457
43	24.115507524257744	25.211783161931322	28.19568880367207	22.477020510138864
44	22.826564406484227	25.345883446632968	28.057637557376097	23.769914589506712
45	22.668990761722156	26.258555574923015	26.68247051304398	24.389983150310847
46	23.508918714775433	25.998256928708386	27.969554354773113	22.52327000174307
47	21.89646156527802	25.843007379001804	30.838184881761666	21.422346173958516
48	22.747080355586544	25.69310324792284	29.20271919121492	22.357097205275693
49	23.367148916390683	24.131776189646157	30.482830747777584	22.018244146185577
50	21.746092615187962	25.770263203765033	29.5480797164604	22.9355644645866
51	21.52809249898321	25.151589099994187	28.244727209342862	25.07559119167974
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	200.0
1	481.5
2	763.0
3	2169.5
4	3576.0
5	2467.0
6	1358.0
7	1244.5
8	1131.0
9	1156.0
10	1181.0
11	1146.0
12	1111.0
13	1135.0
14	1159.0
15	1290.0
16	1421.0
17	1302.5
18	1184.0
19	1172.0
20	1160.0
21	1274.0
22	1388.0
23	1889.5
24	2391.0
25	2738.0
26	3705.5
27	4326.0
28	4985.0
29	5644.0
30	6741.5
31	7839.0
32	9002.0
33	10165.0
34	11762.5
35	13360.0
36	13259.5
37	13159.0
38	15015.0
39	16871.0
40	18704.0
41	20537.0
42	22981.0
43	25425.0
44	27730.0
45	30035.0
46	33070.5
47	36106.0
48	36084.5
49	36063.0
50	35667.0
51	35271.0
52	32148.5
53	29026.0
54	26603.5
55	24181.0
56	23036.0
57	21891.0
58	20418.0
59	18945.0
60	18129.5
61	17314.0
62	15892.0
63	14470.0
64	12230.5
65	9991.0
66	8307.5
67	6624.0
68	5694.0
69	4764.0
70	3825.0
71	2886.0
72	2402.5
73	1919.0
74	1565.5
75	841.5
76	471.0
77	416.0
78	361.0
79	251.0
80	141.0
81	118.5
82	96.0
83	63.0
84	30.0
85	23.0
86	16.0
87	15.0
88	14.0
89	10.0
90	6.0
91	4.5
92	3.0
93	2.5
94	2.0
95	1.5
96	1.0
97	1.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	430275.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.43843440266331
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.01582117757872	24.009573600281453
2	9.2442635000825	5.997388702967219
3	3.6195871328103757	3.5224121931718066
4	2.1015472759585383	2.726836138211073
5	1.4044025327028928	2.2778309716008502
6	1.0589311330110671	2.0610040857070464
7	0.8070249304536221	1.8325037687483618
8	0.6373903741047356	1.6540756671428396
9	0.5096706047461411	1.4879624831121094
>10	6.3397448630347135	46.95072972987472
>50	0.22649535962547465	4.459676502511025
>100	0.030820571088276617	1.5398799580903246
>500	0.002150272401507671	0.5594067913581944
>1k	0.002150272401507671	0.9207194072229634
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1870	0.43460577537621287	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGC	1083	0.25169949450932544	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCG	1007	0.23403637208761838	No Hit
GCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCTGC	887	0.20614723142176514	TruSeq Adapter, Index 21 (95% over 22bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	762	0.17709604322816802	No Hit
GAATCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTC	757	0.17593399570042415	No Hit
CCTGTCTCTTATACACATCTGACGCTAGAGGTGTCGTATGCCGTCTTCTGC	473	0.1099296961245715	TruSeq Adapter, Index 21 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.10737319156353495	0.0
2	0.0	0.0	0.0	0.6082156760211492	0.0
3	0.0	0.0	0.0	0.8020452036488293	0.0
4	0.0	0.0	0.0	1.0651327639300447	0.0
5	0.0	0.0	0.0	1.9273720295160073	0.0
6	0.0	0.0	0.0	2.332926616698623	0.0
7	0.0	0.0	0.0	2.748474812619836	0.0
8	0.0	0.0	0.0	3.411306722444948	0.0
9	0.0	0.0	0.0	3.662308988437627	0.0
10	0.0	0.0	0.0	4.479228400441578	0.0
11	0.0	0.0	0.0	5.331009238277845	0.0
12	0.0	0.0	0.0	6.28412062053338	0.0
13	0.0	0.0	0.0	6.625995003195631	0.0
14	0.0	0.0	0.0	6.777758410318982	0.0
15	0.0	0.0	0.0	7.079193539015746	0.0
16	0.0	0.0	0.0	7.6295392481552495	0.0
17	0.0	0.0	0.0	8.24310034280402	0.0
18	0.0	0.0	0.0	8.928011155656266	0.0
19	0.0	0.0	0.0	9.334960199872175	0.0
20	0.0	0.0	0.0	9.754459357387717	0.0
21	0.0	0.0	0.0	10.224158968101795	0.0
22	0.0	0.0	0.0	10.77032130614142	0.0
23	0.0	0.0	0.0	11.292545465109523	0.0
24	0.0	0.0	0.0	11.714136308175004	0.0
25	0.0	0.0	0.0	12.083667421997559	0.0
26	0.0	0.0	0.0	12.43507059438731	0.0
27	0.0	0.0	0.0	12.826913020742548	0.0
28	0.0	0.0	0.0	13.197838591598396	0.0
29	0.0	0.0	0.0	13.572947533554123	0.0
30	0.0	0.0	0.0	14.05961303817326	0.0
31	0.0	0.0	0.0	14.470513043983498	0.0
32	0.0	0.0	0.0	14.845854395444773	0.0
33	0.0	0.0	0.0	15.232816222183487	0.0
34	0.0	0.0	0.0	15.638370809366103	0.0
35	0.0	0.0	0.0	16.081575736447622	0.0
36	0.0	0.0	0.0	16.48666550461914	0.0
37	0.0	0.0	0.0	16.880599616524314	0.0
38	0.0	0.0	0.0	17.346813084655164	0.0
39	0.0	0.0	0.0	17.96107140782058	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACGAC	30	2.1623237E-6	45.000004	26
TCGTCAG	30	2.1623237E-6	45.000004	1
CGCGGAT	25	3.8867227E-5	45.0	37
CAGCCCG	20	7.0283614E-4	45.0	13
AGCCCGA	20	7.0283614E-4	45.0	14
TAGCGGC	20	7.0283614E-4	45.0	43
GTTAGCG	25	3.8867227E-5	45.0	1
TTATCCG	20	7.0283614E-4	45.0	21
GTAATCT	20	7.0283614E-4	45.0	19
ATATCCG	20	7.0283614E-4	45.0	31
CCATCCG	20	7.0283614E-4	45.0	29
TGCGAGC	25	3.8867227E-5	45.0	13
TGCGACC	25	3.8867227E-5	45.0	11
CCGAGTT	20	7.0283614E-4	45.0	32
AACCTAT	20	7.0283614E-4	45.0	31
CCGCGGA	25	3.8867227E-5	45.0	36
GGACCGT	20	7.0283614E-4	45.0	8
AATAGCG	25	3.8867227E-5	45.0	1
TAATGCG	25	3.8867227E-5	45.0	1
TACGTTG	20	7.0283614E-4	45.0	1
>>END_MODULE