##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936734.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	448532
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.083055389582015	31.0	31.0	33.0	28.0	34.0
2	31.117817680789777	31.0	31.0	34.0	28.0	34.0
3	30.875645438898452	31.0	31.0	34.0	27.0	34.0
4	34.84910998546369	35.0	35.0	37.0	32.0	37.0
5	35.13446755192495	35.0	35.0	37.0	33.0	37.0
6	35.11214361517127	36.0	35.0	37.0	32.0	37.0
7	31.234337795296657	35.0	35.0	37.0	0.0	37.0
8	33.183737169254364	36.0	35.0	37.0	17.0	37.0
9	36.382895757716284	39.0	35.0	39.0	32.0	39.0
10	36.11692365316187	37.0	35.0	39.0	32.0	39.0
11	36.357831771200274	38.0	35.0	39.0	32.0	39.0
12	36.36566621779494	38.0	35.0	39.0	32.0	39.0
13	36.29421535141305	38.0	35.0	39.0	32.0	39.0
14	37.32887062684491	39.0	36.0	40.0	32.0	41.0
15	37.52859773661634	39.0	36.0	41.0	33.0	41.0
16	37.46401817484594	39.0	36.0	40.0	33.0	41.0
17	37.335724095493745	39.0	36.0	40.0	32.0	41.0
18	37.2294329055675	39.0	36.0	40.0	32.0	41.0
19	37.05142553931492	39.0	35.0	40.0	32.0	41.0
20	37.000118163252566	39.0	35.0	40.0	32.0	41.0
21	36.99254902660234	39.0	35.0	40.0	32.0	41.0
22	37.079691081126875	39.0	35.0	40.0	32.0	41.0
23	37.15055559023659	39.0	35.0	40.0	32.0	41.0
24	37.06035243862199	39.0	35.0	40.0	32.0	41.0
25	36.73462317069908	38.0	35.0	40.0	31.0	41.0
26	36.7441631812223	38.0	35.0	40.0	31.0	41.0
27	36.742343913031846	38.0	35.0	40.0	31.0	41.0
28	36.7727653768293	39.0	35.0	40.0	31.0	41.0
29	36.77507513399267	39.0	35.0	40.0	31.0	41.0
30	36.48314501529434	38.0	35.0	40.0	31.0	41.0
31	36.44761800718789	38.0	35.0	40.0	30.0	41.0
32	36.27433717103796	38.0	35.0	40.0	30.0	41.0
33	36.06058653563179	38.0	35.0	40.0	30.0	41.0
34	35.932062818260455	38.0	35.0	40.0	29.0	41.0
35	35.828493842133895	38.0	35.0	40.0	29.0	41.0
36	35.746031498310046	38.0	35.0	40.0	29.0	41.0
37	35.691310764895256	38.0	35.0	40.0	28.0	41.0
38	35.58584225874631	38.0	35.0	40.0	27.0	41.0
39	35.54085104295792	38.0	35.0	40.0	27.0	41.0
40	35.41951075954447	38.0	34.0	40.0	27.0	41.0
41	35.2550208234864	38.0	34.0	40.0	26.0	41.0
42	35.28997485129266	38.0	34.0	40.0	26.0	41.0
43	35.17984669990101	38.0	34.0	40.0	26.0	41.0
44	34.97487358761471	38.0	34.0	40.0	25.0	41.0
45	34.90103270223752	38.0	34.0	40.0	24.0	41.0
46	34.76898415274719	38.0	34.0	40.0	24.0	41.0
47	34.73448940097919	38.0	34.0	40.0	24.0	41.0
48	34.65868655971035	37.0	34.0	40.0	24.0	41.0
49	34.51322536630608	37.0	34.0	40.0	24.0	41.0
50	34.3509337126448	37.0	33.0	40.0	23.0	41.0
51	32.693636128525945	35.0	31.0	39.0	19.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	15.0
9	19.0
10	30.0
11	37.0
12	19.0
13	15.0
14	16.0
15	27.0
16	55.0
17	105.0
18	221.0
19	416.0
20	725.0
21	1163.0
22	1692.0
23	2503.0
24	3613.0
25	4961.0
26	6537.0
27	7641.0
28	8228.0
29	8993.0
30	10650.0
31	13310.0
32	17182.0
33	24005.0
34	37744.0
35	39709.0
36	43218.0
37	63605.0
38	84905.0
39	67167.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.13998555287025	21.95361757912479	23.877449100621583	13.028947767383375
2	35.310301160229365	23.737436793807355	24.001631990582613	16.950630055380664
3	32.63735920736982	22.06687594196178	29.25432299144766	16.041441859220747
4	29.1029402584431	25.055068534686487	27.5159854815264	18.32600572534401
5	25.90428330643076	29.306716131736422	26.43445729624642	18.3545432655864
6	25.335539047381232	34.394424478075145	26.61125627602936	13.658780198514265
7	67.42239126751268	17.367768631892485	11.30933801824619	3.90050208234864
8	76.9146905906379	6.867960368490989	11.617008373984465	4.600340666886644
9	71.41207316311879	8.781313261930029	13.640721286329626	6.1658922886215475
10	40.87066251683269	25.83293945582478	20.757270384275817	12.539127643066717
11	32.62130684098348	24.964996923296443	26.162235916277993	16.25146031944209
12	30.092167337001598	22.472867041816414	30.37419849642835	17.06076712475364
13	24.369721669802825	24.96789526722731	31.46665120883237	19.195731854137495
14	19.268413401942336	28.48826839556598	31.247268868218992	20.996049334272694
15	19.38144881524618	26.41015579713376	36.38937690064477	17.81901848697529
16	21.913486663158928	25.683340319085374	33.86023739666289	18.54293562109281
17	21.82163145550373	24.94961340550953	30.805382893528222	22.423372245458516
18	22.593928638313432	25.912532439157072	32.08511321377293	19.408425708756567
19	24.79154218651066	26.85739256061998	28.826259887811794	19.524805365057567
20	26.417290182194357	25.929476603675994	29.624642165999305	18.028591048130345
21	24.019245003700963	27.57529005734262	29.549508173329887	18.855956765626534
22	22.42961483238654	25.892466981174138	29.920273246947822	21.757644939491495
23	22.387700320155528	26.1408327610962	30.459588167622375	21.011878751125895
24	22.893795760391676	24.21923073493084	32.148876780252024	20.73809672442546
25	21.594891780296614	26.242274798676572	29.567790035047665	22.59504338597915
26	21.00920335672817	26.47258166641399	30.909723275039465	21.608491701818377
27	21.20183175336431	27.837924607385872	30.460034066688664	20.500209572561154
28	18.48965068267147	28.290735109200682	31.389733619897797	21.82988058823005
29	21.182881043047097	26.88169405973264	30.952306635869903	20.983118261350363
30	21.660661892573994	25.96581737757841	31.503883780867366	20.869636948980226
31	23.31896052009667	26.428660608384686	28.558943397572527	21.693435473946117
32	23.982458330732257	27.22057735011103	28.609775891129285	20.18718842802743
33	23.180285910481306	28.14938510518759	27.860665459766526	20.809663524564577
34	21.286552575958908	27.289468755852425	30.615875790356096	20.808102877832575
35	21.070068579276395	26.261894357593214	29.987158106890927	22.680878956239468
36	22.055282566238308	29.72430060731453	28.123077060276636	20.09733976617053
37	21.0098722053276	28.74778165214522	28.921013439397857	21.32133270312932
38	20.7842472777862	29.28397527935577	26.729196579062364	23.20258086379567
39	23.193216983403637	25.731720367777548	28.8226926952815	22.25236995353732
40	22.7254688628682	25.293401585617083	30.92577564142581	21.05535391008891
41	19.459481151846468	26.89707757751955	30.37308374876263	23.27035752187135
42	21.454210624882954	26.775793031489393	28.772974949390456	22.997021394237198
43	22.483791568940454	25.85434261100657	28.18461113142429	23.477254688628683
44	20.219962009399552	26.398339471877147	28.523717371335827	24.857981147387477
45	20.598976215743807	26.337697198862063	27.9427108879634	25.120615697430733
46	21.35633577983288	27.157928531297653	28.70988023151079	22.775855457358674
47	20.718477165508816	26.384070701755952	30.683652448431776	22.213799684303464
48	20.809886474097723	24.47852104197694	31.542008151034935	23.16958433289041
49	20.536773295996717	24.597799042208806	31.125092524056253	23.74033513773822
50	19.51655623233125	25.049271846824755	31.37278945537888	24.061382465465115
51	18.77168184209822	24.44597041013796	30.66113454558426	26.121213202179554
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	182.0
1	516.5
2	851.0
3	4396.5
4	7942.0
5	5307.0
6	2672.0
7	2498.5
8	2325.0
9	2139.0
10	1953.0
11	1911.0
12	1869.0
13	1786.5
14	1704.0
15	1627.5
16	1551.0
17	1552.0
18	1553.0
19	1697.5
20	1842.0
21	1779.0
22	1716.0
23	2097.0
24	2478.0
25	2963.0
26	4066.0
27	4684.0
28	5973.0
29	7262.0
30	8186.0
31	9110.0
32	10348.5
33	11587.0
34	12057.5
35	12528.0
36	13463.0
37	14398.0
38	15600.5
39	16803.0
40	19057.5
41	21312.0
42	24425.5
43	27539.0
44	29344.5
45	31150.0
46	34534.0
47	37918.0
48	38888.0
49	39858.0
50	37697.5
51	35537.0
52	31612.0
53	27687.0
54	25535.5
55	23384.0
56	21638.0
57	19892.0
58	19619.5
59	19347.0
60	18934.5
61	18522.0
62	16867.5
63	15213.0
64	12441.5
65	9670.0
66	7501.5
67	5333.0
68	4183.0
69	3033.0
70	2430.0
71	1827.0
72	1566.0
73	1305.0
74	984.5
75	630.5
76	597.0
77	373.0
78	149.0
79	102.0
80	55.0
81	46.0
82	37.0
83	30.0
84	23.0
85	17.5
86	12.0
87	8.5
88	5.0
89	2.5
90	0.0
91	1.5
92	3.0
93	2.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	448532.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.58893443218689
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.72564533479522	25.099548015532886
2	9.678732278814714	6.501966077995965
3	3.7337349992034663	3.7623654022621973
4	1.9864059166764192	2.6688503236380936
5	1.290204215663306	2.166829240202295
6	0.9468533828945249	1.9082277716963123
7	0.6986423242606941	1.6426655854800176
8	0.5709919321346859	1.5343208455823738
9	0.44870040407091794	1.3564231606830441
>10	5.633232148228835	44.065820051538246
>50	0.24313289195240684	5.034125910992395
>100	0.03908675919670846	2.2993247154791323
>500	0.003312437220060039	0.719415155825417
>1k	0.0013249748880240155	1.240117743091571
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4310	0.9609124878492504	No Hit
GCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCTGC	1263	0.28158526036046483	TruSeq Adapter, Index 27 (95% over 21bp)
GAATGATACCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCG	754	0.1681039479903329	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGC	752	0.16765804892404554	No Hit
GAATCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTC	641	0.14291065074509735	No Hit
TGTTGTGAGGATTAAAATGAGAAAATGAATGTAAAACACTTAGCATGGTGT	562	0.1252976376267468	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	524	0.11682555536728706	No Hit
CCTGTCTCTTATACACATCTGACGCGACCATTGTCGTATGCCGTCTTCTGC	480	0.10701577590896524	TruSeq Adapter, Index 27 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.12016979836444222	0.0
2	0.0	0.0	0.0	0.7854512052651762	0.0
3	0.0	0.0	0.0	1.0264596505934918	0.0
4	0.0	0.0	0.0	1.3664576886376	0.0
5	0.0	0.0	0.0	2.3398553503428965	0.0
6	0.0	0.0	0.0	2.8764948766197285	0.0
7	0.0	0.0	0.0	3.377239528060428	0.0
8	0.0	0.0	0.0	4.2358181801967305	0.0
9	0.0	0.0	0.0	4.539252494805276	0.0
10	0.0	0.0	0.0	5.386014821684963	0.0
11	0.0	0.0	0.0	6.407569582549294	0.0
12	0.0	0.0	0.0	7.371603363862556	0.0
13	0.0	0.0	0.0	7.778932160916055	0.0
14	0.0	0.0	0.0	7.971560557552192	0.0
15	0.0	0.0	0.0	8.298627522673968	0.0
16	0.0	0.0	0.0	8.940722178127759	0.0
17	0.0	0.0	0.0	9.62941328600858	0.0
18	0.0	0.0	0.0	10.454103609107042	0.0
19	0.0	0.0	0.0	10.930100862368795	0.0
20	0.0	0.0	0.0	11.409888257693988	0.0
21	0.0	0.0	0.0	11.979970213942371	0.0
22	0.0	0.0	0.0	12.546484977660457	0.0
23	0.0	0.0	0.0	13.122586571303719	0.0
24	0.0	0.0	0.0	13.606832957291788	0.0
25	2.229495331436776E-4	0.0	0.0	14.024863331936183	0.0
26	2.229495331436776E-4	0.0	0.0	14.42238234953136	0.0
27	2.229495331436776E-4	0.0	0.0	14.790026129685284	0.0
28	2.229495331436776E-4	0.0	0.0	15.218758081920576	0.0
29	2.229495331436776E-4	0.0	0.0	15.61850659484719	0.0
30	2.229495331436776E-4	0.0	0.0	16.06284501440254	0.0
31	2.229495331436776E-4	0.0	0.0	16.524573497543095	0.0
32	2.229495331436776E-4	0.0	0.0	16.97827579749048	0.0
33	2.229495331436776E-4	0.0	0.0	17.379362007615956	0.0
34	2.229495331436776E-4	0.0	0.0	17.80341201965523	0.0
35	2.229495331436776E-4	0.0	0.0	18.24730454014429	0.0
36	2.229495331436776E-4	0.0	0.0	18.69877734476024	0.0
37	2.229495331436776E-4	0.0	0.0	19.152033745641337	0.0
38	2.229495331436776E-4	0.0	0.0	19.610863884851025	0.0
39	2.229495331436776E-4	0.0	0.0	20.07125467079272	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACTTGCG	35	1.2097189E-7	45.000004	1
TAATACG	25	3.8869883E-5	45.0	1
GTACCGG	20	7.028681E-4	45.0	2
CGACCGG	20	7.028681E-4	45.0	2
GCACGCT	20	7.028681E-4	45.0	41
CCGCTTG	20	7.028681E-4	45.0	39
ACGGGTA	55	1.8189894E-12	45.0	5
ATCCGGT	20	7.028681E-4	45.0	11
GTTACCG	20	7.028681E-4	45.0	41
CGTACTT	20	7.028681E-4	45.0	35
GTATCCG	20	7.028681E-4	45.0	9
ATGCGAC	20	7.028681E-4	45.0	12
CGGTCAA	20	7.028681E-4	45.0	45
TATCGAG	20	7.028681E-4	45.0	1
TGTCGCT	20	7.028681E-4	45.0	27
TAGTCCG	25	3.8869883E-5	45.0	19
GCGTAAG	55	1.8189894E-12	45.0	1
CGCTTCA	25	3.8869883E-5	45.0	44
TGCAACG	20	7.028681E-4	45.0	1
TGATCCA	30	2.162531E-6	44.999996	38
>>END_MODULE