##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936732.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	304662
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.145817331994145	31.0	31.0	33.0	28.0	34.0
2	31.21165750897716	31.0	31.0	34.0	28.0	34.0
3	30.881068200169366	31.0	31.0	34.0	27.0	34.0
4	34.820814542017054	35.0	35.0	37.0	32.0	37.0
5	35.150166413927565	35.0	35.0	37.0	33.0	37.0
6	35.1834459171147	36.0	35.0	37.0	33.0	37.0
7	30.656127117920843	35.0	35.0	37.0	0.0	37.0
8	32.971286212261454	36.0	35.0	37.0	17.0	37.0
9	36.41838496432111	39.0	35.0	39.0	32.0	39.0
10	36.239478504047106	37.0	35.0	39.0	32.0	39.0
11	36.43378235552842	38.0	35.0	39.0	32.0	39.0
12	36.45990310573685	38.0	35.0	39.0	32.0	39.0
13	36.383424910228385	39.0	35.0	39.0	32.0	39.0
14	37.410415476823495	39.0	36.0	41.0	32.0	41.0
15	37.6605976459158	39.0	36.0	41.0	33.0	41.0
16	37.586305479515005	39.0	36.0	41.0	33.0	41.0
17	37.51951014566963	39.0	36.0	41.0	33.0	41.0
18	37.36878573632419	39.0	36.0	40.0	32.0	41.0
19	37.15071784469346	39.0	36.0	40.0	32.0	41.0
20	37.05151938869961	39.0	35.0	40.0	32.0	41.0
21	37.033059587345974	39.0	35.0	40.0	32.0	41.0
22	37.105251065114786	39.0	35.0	40.0	32.0	41.0
23	37.16662399642883	39.0	35.0	40.0	32.0	41.0
24	37.06892228108527	39.0	35.0	40.0	32.0	41.0
25	36.76199526032127	38.0	35.0	40.0	31.0	41.0
26	36.766081756175694	38.0	35.0	40.0	32.0	41.0
27	36.76621633154118	38.0	35.0	40.0	32.0	41.0
28	36.79369924703442	39.0	35.0	40.0	32.0	41.0
29	36.77251839743716	39.0	35.0	40.0	32.0	41.0
30	36.48015177475366	38.0	35.0	40.0	31.0	41.0
31	36.3920771215314	38.0	35.0	40.0	31.0	41.0
32	36.12361896134077	38.0	35.0	40.0	30.0	41.0
33	35.88812520104247	38.0	35.0	40.0	29.0	41.0
34	35.69571853398192	38.0	35.0	40.0	28.0	41.0
35	35.55172617523682	38.0	35.0	40.0	27.0	41.0
36	35.447213633469225	38.0	35.0	40.0	27.0	41.0
37	35.30632635510828	38.0	35.0	40.0	26.0	41.0
38	35.20941896265369	38.0	34.0	40.0	25.0	41.0
39	35.18749302505728	38.0	34.0	40.0	25.0	41.0
40	35.10451582409359	38.0	34.0	40.0	25.0	41.0
41	35.00432282332552	38.0	34.0	40.0	24.0	41.0
42	34.95534723726622	38.0	34.0	40.0	24.0	41.0
43	34.85086751875849	38.0	34.0	40.0	24.0	41.0
44	34.62661244264135	38.0	34.0	40.0	23.0	41.0
45	34.55770329086003	38.0	34.0	40.0	23.0	41.0
46	34.468483105868145	38.0	34.0	40.0	23.0	41.0
47	34.43307665544111	37.0	33.0	40.0	23.0	41.0
48	34.35306667717011	37.0	33.0	40.0	23.0	41.0
49	34.25246995030558	37.0	33.0	40.0	22.0	41.0
50	34.06343423203419	37.0	33.0	40.0	22.0	41.0
51	32.467957933710146	35.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	10.0
9	20.0
10	24.0
11	10.0
12	10.0
13	2.0
14	10.0
15	14.0
16	39.0
17	79.0
18	168.0
19	284.0
20	476.0
21	797.0
22	1247.0
23	1840.0
24	2651.0
25	3957.0
26	5114.0
27	5660.0
28	5968.0
29	6232.0
30	7321.0
31	8985.0
32	11318.0
33	15846.0
34	25392.0
35	28325.0
36	28684.0
37	43078.0
38	56373.0
39	44728.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.20693095955518	20.426242852735164	24.268533653688348	17.098292534021308
2	36.464015860199176	22.672338525973046	24.79436227688389	16.069283336943894
3	30.896534520222414	21.79333162652382	31.67575870965201	15.634375143601762
4	26.121078441026448	25.366143463904262	31.145663062672735	17.36711503239656
5	24.01645101784929	28.5903722814135	30.414689065259203	16.978487635478004
6	24.732654548319122	32.754659261739235	29.25208920049104	13.260596989450605
7	65.1285687089299	18.60980365125943	12.676014731079032	3.585612908731644
8	78.09572575509908	4.404881475208592	13.147028510283526	4.3523642594087875
9	72.30176392198567	6.858420150855703	15.732844923226393	5.106971003932227
10	40.14514445516671	25.6510493596182	21.670572634591778	12.533233550623313
11	29.244211618121064	25.971076143398257	28.49354366478261	16.291168573698066
12	26.783123592702733	22.579120467928394	33.28081611753353	17.356939821835347
13	21.900007221117175	25.93595525533214	33.97732569207844	18.186711831472255
14	17.39074777950647	29.11193388082531	34.175906414321446	19.321411925346776
15	17.698301724534073	25.881140411341093	38.611313521213674	17.80924434291116
16	20.400968942631508	23.27530180987455	37.53602352771268	18.78770571978127
17	20.043851875192836	23.35342116837676	32.898425139991204	23.7043018164392
18	20.81585494744996	24.47466372570258	35.02898293846952	19.68049838837794
19	22.0677340790778	25.76560253658152	33.21320020219128	18.9534631821494
20	23.489309464258753	25.094695104739024	32.56428435446495	18.851711076537278
21	22.40581365578904	24.93418936395087	33.81846111428403	18.841535865976063
22	20.88051676940347	23.48176011448753	33.64810839553341	21.989614720575588
23	20.63368585514439	24.21765760088229	33.227970669134976	21.920685874838345
24	20.183022497062318	23.532307934694842	35.034562892648246	21.250106675594594
25	19.01779677150416	24.92598354898215	33.840780930998946	22.21543874851475
26	18.07281511970643	28.03073570054684	33.17414052294018	20.72230865680656
27	17.607381294680664	28.132816038757706	33.84734558297392	20.41245708358771
28	17.004089778180408	26.337711956200643	36.46270292980417	20.195495335814773
29	17.595893153724454	25.568991209931006	35.18456519027644	21.650550446068102
30	19.112984225141304	26.589794592039702	35.15403955859281	19.14318162422619
31	21.791362230931327	26.89111211769108	31.164044088202665	20.15348156317493
32	22.435026357077682	26.962338591619567	30.53941745278374	20.063217598519014
33	21.145400476593736	26.457188622145196	32.13823844128903	20.259172459972035
34	19.053902357366525	26.127314860402677	32.27839376095476	22.540389021276034
35	19.7320309063815	26.984001943136988	30.572568945257366	22.71139820522415
36	22.329007227681824	27.2344434159823	31.12038915256908	19.316160203766795
37	19.974923029455592	27.918480151774755	31.97576330490839	20.130833513861262
38	20.19319770762353	27.733028733481696	30.07496832555422	21.998805233340555
39	20.263767716354515	26.787062383887715	31.249712796476093	21.69945710328167
40	20.780405826785092	25.414721888519082	31.531664598801296	22.273207685894533
41	19.44220152168633	25.813196263400094	30.093349351084154	24.651252863829423
42	21.12669121846505	25.508924644359983	29.902646211211113	23.461737925963856
43	21.24288555842212	26.190991984559936	29.965995102769625	22.600127354248315
44	19.25871949898576	26.16670277225253	31.351793134686968	23.222784594074746
45	19.941771536981967	26.720759398940462	29.523865792255023	23.813603271822544
46	21.185116621042337	26.653799948795715	30.48854139997768	21.672542030184268
47	19.52787022995976	24.996881790311885	32.459906388062834	23.015341591665518
48	19.960152562511897	24.431008790068994	32.96243049674722	22.646408150671892
49	20.652723345871816	23.982971292776913	33.40259041166932	21.961714949681944
50	19.362112767591626	24.820949117382543	32.76910149608418	23.04783661894165
51	18.95083732135941	25.0205145374218	30.81381990533772	25.214828235881075
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	130.0
1	396.0
2	662.0
3	4332.5
4	8003.0
5	5240.5
6	2478.0
7	2345.5
8	2213.0
9	2130.5
10	2048.0
11	2040.0
12	2032.0
13	1889.5
14	1747.0
15	1670.0
16	1593.0
17	1559.0
18	1525.0
19	1554.0
20	1583.0
21	1589.5
22	1596.0
23	1832.5
24	2069.0
25	2267.5
26	3029.0
27	3592.0
28	4044.0
29	4496.0
30	5100.0
31	5704.0
32	6647.0
33	7590.0
34	8589.0
35	9588.0
36	10148.0
37	10708.0
38	11735.5
39	12763.0
40	14193.0
41	15623.0
42	17787.0
43	19951.0
44	21536.5
45	23122.0
46	24383.0
47	25644.0
48	26386.5
49	27129.0
50	25781.0
51	24433.0
52	21291.5
53	18150.0
54	16576.0
55	15002.0
56	13625.5
57	12249.0
58	10980.0
59	9711.0
60	9228.5
61	8746.0
62	8052.5
63	7359.0
64	6070.5
65	4782.0
66	3927.0
67	3072.0
68	2493.5
69	1915.0
70	1596.0
71	1277.0
72	1029.5
73	782.0
74	662.5
75	426.0
76	309.0
77	198.5
78	88.0
79	78.0
80	68.0
81	57.0
82	46.0
83	45.5
84	45.0
85	31.5
86	18.0
87	14.0
88	10.0
89	5.0
90	0.0
91	1.0
92	2.0
93	1.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	304662.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.48865563432701
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.68704910166466	25.068786055427307
2	10.090297148149565	6.960015671811244
3	3.940004850184094	4.076564114267321
4	2.3461213174184645	3.2365828077119607
5	1.4202932987829189	2.44920032407332
6	1.0661280858932147	2.2061594649873175
7	0.8367348975268736	2.020050321661977
8	0.6332843203058086	1.74728998733167
9	0.529565137550202	1.6437590698422537
>10	6.301924477997728	44.29378371086333
>50	0.12266762306548423	2.680782044289562
>100	0.02194055046699718	1.2740077870218807
>500	0.0029918932454996157	0.6885970814302929
>1k	9.97297748499872E-4	1.654421559280574
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4810	1.5787987999816189	No Hit
GCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTGC	839	0.2753871503502242	No Hit
CCTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTGC	620	0.20350421122424192	No Hit
TTATATGGGAACATATCCTTCCTCCTTTAAACTCATAAACTATTTTTTGAC	543	0.1782303011205861	No Hit
CTGTCTCTTATACACATCTGACGCAATCTCGGTCGTATGCCGTCTTCTGCT	471	0.15459755401067413	Illumina Single End Adapter 2 (95% over 21bp)
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	372	0.12210252673454516	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.17855853371933486	0.0
2	0.0	0.0	0.0	0.9118301593241034	0.0
3	0.0	0.0	0.0	1.1885302400693227	0.0
4	0.0	0.0	0.0	1.5574636810629485	0.0
5	0.0	0.0	0.0	2.4197307179759866	0.0
6	0.0	0.0	0.0	2.8529977483243725	0.0
7	0.0	0.0	0.0	3.291516500252739	0.0
8	0.0	0.0	0.0	4.129166092259619	0.0
9	0.0	0.0	0.0	4.420636639948533	0.0
10	0.0	0.0	0.0	5.144389520189587	0.0
11	0.0	0.0	0.0	6.2935318484090566	0.0
12	0.0	0.0	0.0	7.173523445654529	0.0
13	0.0	0.0	0.0	7.5979281958366975	0.0
14	0.0	0.0	0.0	7.782066683734762	0.0
15	0.0	0.0	0.0	8.101437002317322	0.0
16	0.0	0.0	0.0	8.75724573461738	0.0
17	0.0	0.0	0.0	9.540080482633213	0.0
18	0.0	0.0	0.0	10.477184552060972	0.0
19	3.282325987487773E-4	0.0	0.0	10.983975684529085	0.0
20	3.282325987487773E-4	0.0	0.0	11.500285562360911	0.0
21	3.282325987487773E-4	0.0	0.0	12.076333773165016	0.0
22	3.282325987487773E-4	0.0	0.0	12.671747707295298	0.0
23	3.282325987487773E-4	0.0	0.0	13.244185359513166	0.0
24	3.282325987487773E-4	0.0	0.0	13.754587050567514	0.0
25	3.282325987487773E-4	0.0	0.0	14.20951743243332	0.0
26	3.282325987487773E-4	0.0	0.0	14.621121111264287	0.0
27	3.282325987487773E-4	0.0	0.0	14.99858859982538	0.0
28	3.282325987487773E-4	0.0	0.0	15.401986463687628	0.0
29	3.282325987487773E-4	0.0	0.0	15.832955865844772	0.0
30	3.282325987487773E-4	0.0	0.0	16.325961229165436	0.0
31	3.282325987487773E-4	0.0	0.0	16.77498342425376	0.0
32	3.282325987487773E-4	0.0	0.0	17.17575542732602	0.0
33	3.282325987487773E-4	0.0	0.0	17.646440973931767	0.0
34	3.282325987487773E-4	0.0	0.0	18.082005632471393	0.0
35	3.282325987487773E-4	0.0	0.0	18.5369360143372	0.0
36	3.282325987487773E-4	0.0	0.0	18.967905416494343	0.0
37	3.282325987487773E-4	0.0	0.0	19.396905423058996	0.0
38	3.282325987487773E-4	0.0	0.0	19.82853129041364	0.0
39	3.282325987487773E-4	0.0	0.0	20.27361469431698	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTATCC	20	7.025115E-4	45.000004	30
CGGCCTA	20	7.025115E-4	45.000004	39
TTCCGCA	20	7.025115E-4	45.000004	22
CACGTAT	20	7.025115E-4	45.000004	28
TTCGCGA	20	7.025115E-4	45.000004	1
CAATACG	20	7.025115E-4	45.000004	42
TGGTATT	20	7.025115E-4	45.000004	37
CGGCATT	25	3.8840357E-5	44.999996	20
TAGCGGA	30	2.1602318E-6	44.999996	3
TTATCGC	25	3.8840357E-5	44.999996	31
GTAGCGG	60	0.0	44.999996	2
ACGGCAT	25	3.8840357E-5	44.999996	19
TGTAGCG	25	3.8840357E-5	44.999996	1
TCGCGGC	25	3.8840357E-5	44.999996	34
TACGTAG	25	3.8840357E-5	44.999996	1
CGTTTTT	2125	0.0	43.2	1
GTCGGGA	60	3.6379788E-12	41.249996	4
TAGACGG	50	1.0768417E-9	40.499996	2
ACATACG	45	1.9203071E-8	40.000004	17
CTATCTC	80	0.0	39.375004	5
>>END_MODULE