##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936731.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	208827
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.071034875758404	31.0	31.0	33.0	28.0	34.0
2	31.16608005669765	31.0	31.0	34.0	28.0	34.0
3	30.741024867474035	31.0	30.0	34.0	27.0	34.0
4	34.67388795510159	35.0	35.0	37.0	32.0	37.0
5	35.08132569064345	35.0	35.0	37.0	33.0	37.0
6	35.10990437060342	35.0	35.0	37.0	32.0	37.0
7	30.572028521216126	35.0	35.0	37.0	0.0	37.0
8	32.88870692008217	36.0	35.0	37.0	17.0	37.0
9	36.320844526809275	39.0	35.0	39.0	32.0	39.0
10	36.27297236468464	37.0	35.0	39.0	32.0	39.0
11	36.49959057018489	38.0	35.0	39.0	32.0	39.0
12	36.47698812892969	38.0	35.0	39.0	32.0	39.0
13	36.26500883506443	38.0	35.0	39.0	32.0	39.0
14	37.28099814679136	39.0	36.0	41.0	32.0	41.0
15	37.55311813127613	39.0	36.0	41.0	32.0	41.0
16	37.53245509440829	39.0	36.0	41.0	33.0	41.0
17	37.43818088657118	39.0	36.0	41.0	32.0	41.0
18	37.2657414989441	39.0	36.0	40.0	32.0	41.0
19	36.91626561699397	38.0	35.0	40.0	32.0	41.0
20	36.75966230420396	38.0	35.0	40.0	32.0	41.0
21	36.72883295742409	38.0	35.0	40.0	32.0	41.0
22	36.781714050386206	38.0	35.0	40.0	32.0	41.0
23	36.8487647670081	38.0	35.0	40.0	32.0	41.0
24	36.726591867909804	38.0	35.0	40.0	32.0	41.0
25	36.425548420462874	38.0	35.0	40.0	31.0	41.0
26	36.38276659627347	38.0	35.0	40.0	31.0	41.0
27	36.385869643293255	38.0	35.0	40.0	31.0	41.0
28	36.31684121306153	38.0	35.0	40.0	31.0	41.0
29	36.31836400465457	38.0	35.0	40.0	31.0	41.0
30	36.02153457167895	38.0	35.0	40.0	30.0	41.0
31	35.76215719231708	38.0	35.0	40.0	30.0	41.0
32	35.422517203235216	38.0	34.0	40.0	28.0	41.0
33	35.03136088724159	38.0	34.0	40.0	25.0	41.0
34	34.70261508329862	38.0	34.0	40.0	23.0	41.0
35	34.451541227906354	38.0	34.0	40.0	21.0	41.0
36	34.24762123671747	38.0	34.0	40.0	20.0	41.0
37	34.06859745147897	38.0	33.0	40.0	18.0	41.0
38	33.90741618660422	37.0	33.0	40.0	18.0	41.0
39	33.90798603628841	37.0	33.0	40.0	18.0	41.0
40	33.79330259018231	37.0	33.0	40.0	18.0	41.0
41	33.612875729670975	37.0	33.0	40.0	17.0	41.0
42	33.60322180560943	37.0	33.0	40.0	17.0	41.0
43	33.48176241577957	37.0	33.0	40.0	15.0	41.0
44	33.19339932096903	37.0	33.0	40.0	15.0	41.0
45	33.092569447437356	36.0	32.0	40.0	15.0	41.0
46	32.955417642354675	36.0	32.0	40.0	14.0	41.0
47	32.937215973030305	36.0	32.0	40.0	15.0	41.0
48	32.863858600659874	36.0	32.0	40.0	13.0	41.0
49	32.78723057842137	36.0	32.0	40.0	13.0	41.0
50	32.60444291207555	36.0	31.0	40.0	12.0	41.0
51	30.972972843549925	35.0	28.0	38.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	7.0
9	10.0
10	19.0
11	11.0
12	8.0
13	8.0
14	9.0
15	20.0
16	34.0
17	72.0
18	151.0
19	292.0
20	493.0
21	735.0
22	1189.0
23	1816.0
24	2748.0
25	4078.0
26	5232.0
27	5625.0
28	5232.0
29	5261.0
30	5606.0
31	6678.0
32	8298.0
33	11313.0
34	17673.0
35	19859.0
36	18726.0
37	27402.0
38	34440.0
39	25781.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	35.4446503565152	19.43091650026098	23.237895482863806	21.886537660360013
2	39.14436351621198	21.308547266397547	23.667916505049636	15.879172712340836
3	29.051798857427443	20.861765959382648	34.75269002571507	15.333745157474848
4	25.26636881246199	23.09327816805298	34.36720347464648	17.27314954483855
5	21.96459270113539	26.74414706910505	34.169910978944294	17.121349250815268
6	23.073165826258098	30.90069770671417	32.74672336431592	13.279413102711816
7	60.87143903805542	18.3807649393996	17.120870385534438	3.62692563701054
8	72.00026816455727	5.222983618018743	18.25099244829452	4.5257557691294705
9	66.79643915777174	6.702677335785124	20.582587500658438	5.9182960057846925
10	38.19094274207837	23.337020595995728	25.870696796870135	12.601339865055763
11	29.259626389307897	20.798076877032184	32.753427478247545	17.188869255412374
12	28.09837808329383	19.053570658966514	36.918118825630785	15.929932432108876
13	21.144775340353497	25.650418767688087	36.31283311066098	16.891972781297437
14	16.41885388383686	28.50589243728062	36.08106231473899	18.994191364143525
15	14.65758738094212	24.046698942186595	44.96209781302226	16.333615863849023
16	17.523117221432095	21.75197651644663	42.11045506567637	18.614451196444904
17	17.28272685045516	22.07999923381555	37.24614154299971	23.39113237272958
18	20.42408309270353	21.57814841950514	38.92935300511907	19.06841548267226
19	21.132324843051904	24.33976449405489	35.19324608407916	19.334664578814042
20	23.212036757698957	22.285911304572682	36.97606152461128	17.52599041311708
21	20.057751152868164	24.434100954378504	37.535376172621355	17.972771720131973
22	18.899854903819907	22.7753116215815	36.08441437170481	22.24041910289378
23	17.970856259008652	24.592126497052586	35.768363286356646	21.668653957582112
24	20.445632030340903	21.476150114688235	37.47695460836003	20.60126324661083
25	17.962715549234535	23.762731830654083	36.098301464848895	22.17625115526249
26	18.044122646975726	25.89128800394585	36.15720189439105	19.907387454687374
27	18.23997854683542	26.767132602584915	36.0858509675473	18.907037883032366
28	15.188170112102362	24.492043653359	41.14314719839867	19.176639036139964
29	18.16048691021755	21.622204025341553	40.54983311544963	19.66747594899127
30	20.35560535754476	23.494567273388977	36.94876620360394	19.20106116546232
31	20.710923395921025	24.60840791660082	35.63092895075828	19.049739736719868
32	22.136026471672725	25.35783208110062	33.20020878526244	19.30593266196421
33	21.78070843329646	25.24865079707126	33.4425146173627	19.528126152269582
34	19.08709122862465	25.29222753762684	35.371862833828956	20.248818399919553
35	19.513281328563835	25.133244264391095	33.486091357918276	21.86738304912679
36	23.410286983962802	23.968165036130383	34.78668946065404	17.834858519252776
37	20.151608747910952	25.0753015654106	36.60685639309093	18.166233293587513
38	18.54836778769029	26.494658257792338	33.835184147643744	21.121789806873633
39	20.814358296580423	24.268413567211137	35.19659814104498	19.72062999516346
40	22.326135988162452	23.61236813247329	33.62831434632495	20.43318153303931
41	18.09871328899041	24.14151426779104	34.38731581644136	23.37245662677719
42	21.957409721922932	22.77195956461569	33.2873622663736	21.98326844708778
43	22.80308580786967	23.521383729115488	32.69931570151369	20.976214761501147
44	20.37045018125051	25.542674079501214	33.34913588760074	20.737739851647536
45	19.885359651769168	26.439588750496824	30.844191603576164	22.830859994157844
46	22.438190463876797	25.90709055821326	32.04901665014581	19.605702327764128
47	19.123484989967775	24.81431998735796	34.76370392717417	21.2984910955001
48	20.507405651568046	23.032941142668335	34.6497339903365	21.809919215427122
49	20.26605755002945	21.890368582606655	35.56293008088034	22.280643786483548
50	19.47257777969324	23.402625139469514	35.018939121856846	22.105857958980398
51	17.85449199576683	23.986840782082776	32.842975285762854	25.315691936387534
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	141.0
1	268.5
2	396.0
3	4331.0
4	8266.0
5	5575.5
6	2885.0
7	2719.5
8	2554.0
9	2465.5
10	2377.0
11	2308.5
12	2240.0
13	2097.5
14	1955.0
15	1812.0
16	1669.0
17	1509.5
18	1350.0
19	1367.5
20	1385.0
21	1326.5
22	1268.0
23	1347.0
24	1426.0
25	1522.0
26	1832.0
27	2046.0
28	2059.5
29	2073.0
30	2402.0
31	2731.0
32	3367.5
33	4004.0
34	4945.5
35	5887.0
36	6313.0
37	6739.0
38	7065.5
39	7392.0
40	8552.5
41	9713.0
42	11380.0
43	13047.0
44	14301.0
45	15555.0
46	17011.0
47	18467.0
48	18948.0
49	19429.0
50	18653.0
51	17877.0
52	15476.0
53	13075.0
54	11503.5
55	9932.0
56	8751.0
57	7570.0
58	6961.5
59	6353.0
60	6031.0
61	5709.0
62	5117.0
63	4525.0
64	3690.5
65	2856.0
66	2309.5
67	1763.0
68	1386.0
69	1009.0
70	847.0
71	685.0
72	551.0
73	417.0
74	332.0
75	157.5
76	68.0
77	65.0
78	62.0
79	53.5
80	45.0
81	28.5
82	12.0
83	8.0
84	4.0
85	2.0
86	0.0
87	1.0
88	2.0
89	1.0
90	0.0
91	1.5
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	208827.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.800279657324005
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.23407901222976	31.919723024321566
2	8.458721626252904	6.90236415789146
3	3.285134151780475	4.0210317631340775
4	1.7652167789488509	2.8808535294765525
5	1.2124128541583532	2.47333917548976
6	0.8098401445975447	1.9825022626384519
7	0.6161827187155231	1.7598299070522487
8	0.48003567991361706	1.5668471988775399
9	0.4401305133682308	1.6161703228030857
>10	4.576183657660618	37.833709242578784
>50	0.1009365977324476	2.443170662797435
>100	0.01760522053472923	1.712422244250025
>500	0.002347362737963898	0.6531722430528619
>1k	0.001173681368981949	2.2348642656361486
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4667	2.2348642656361486	No Hit
GCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGC	727	0.34813505916380544	No Hit
CCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGC	637	0.3050371838890565	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	479	0.22937646951783053	No Hit
CTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGCT	450	0.21548937637374477	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCG	389	0.1862785942430816	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGC	384	0.1838842678389289	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	368	0.17622242334564017	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTC	238	0.11396993683766944	No Hit
TCTGTCTCTTATACACATCTGACGCGGTTGTCTTCGTATGCCGTCTTCTGC	229	0.10966014931019455	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2624181738951381	0.0
2	0.0	0.0	0.0	1.3001192374549269	0.0
3	0.0	0.0	0.0	1.6018043643781694	0.0
4	0.0	0.0	0.0	1.9743615528643326	0.0
5	0.0	0.0	0.0	2.9933868704717304	0.0
6	0.0	0.0	0.0	3.45118207894573	0.0
7	0.0	0.0	0.0	4.013369918640789	0.0
8	0.0	0.0	0.0	5.052507578043069	0.0
9	0.0	0.0	0.0	5.43176888046086	0.0
10	0.0	0.0	0.0	6.574820305803368	0.0
11	0.0	0.0	0.0	8.009500687171679	0.0
12	0.0	0.0	0.0	9.332605458106471	0.0
13	0.0	0.0	0.0	9.81578052646449	0.0
14	0.0	0.0	0.0	10.00062252486508	0.0
15	4.7886528083054396E-4	0.0	0.0	10.357856024364665	0.0
16	4.7886528083054396E-4	0.0	0.0	11.037365857863207	0.0
17	4.7886528083054396E-4	0.0	0.0	11.875858964597489	0.0
18	4.7886528083054396E-4	0.0	0.0	12.828322008169442	0.0
19	4.7886528083054396E-4	0.0	0.0	13.376622754720415	0.0
20	4.7886528083054396E-4	0.0	0.0	13.923965770709726	0.0
21	4.7886528083054396E-4	0.0	0.0	14.542180848261959	0.0
22	4.7886528083054396E-4	0.0	0.0	15.192479899629838	0.0
23	4.7886528083054396E-4	0.0	0.0	15.800159941003798	0.0
24	4.7886528083054396E-4	0.0	0.0	16.291954584416764	0.0
25	4.7886528083054396E-4	0.0	0.0	16.696116881437746	0.0
26	4.7886528083054396E-4	0.0	0.0	17.130447691151048	0.0
27	4.7886528083054396E-4	0.0	0.0	17.52599041311708	0.0
28	4.7886528083054396E-4	0.0	0.0	17.9167444822748	0.0
29	4.7886528083054396E-4	0.0	0.0	18.324258836261595	0.0
30	4.7886528083054396E-4	0.0	0.0	18.798335464283834	0.0
31	4.7886528083054396E-4	0.0	0.0	19.243201310175408	0.0
32	4.7886528083054396E-4	0.0	0.0	19.644969280792235	0.0
33	4.7886528083054396E-4	0.0	0.0	20.066370727923115	0.0
34	9.577305616610879E-4	0.0	0.0	20.45089954843004	0.0
35	9.577305616610879E-4	0.0	0.0	20.89815972072577	0.0
36	9.577305616610879E-4	0.0	0.0	21.307589535835884	0.0
37	9.577305616610879E-4	0.0	0.0	21.714625024541846	0.0
38	9.577305616610879E-4	0.0	0.0	22.1403362592002	0.0
39	9.577305616610879E-4	0.0	0.0	22.57131501194769	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAAATGC	20	7.0200174E-4	45.0	42
CTATGAT	35	1.2055534E-7	45.0	32
CGGAACA	20	7.0200174E-4	45.0	6
CTGTCGG	20	7.0200174E-4	45.0	1
CAAGTCT	20	7.0200174E-4	45.0	25
CCGATCT	20	7.0200174E-4	45.0	11
CGTGCGG	25	3.8798105E-5	45.0	2
TTAGCGG	20	7.0200174E-4	45.0	2
ATTGTCG	20	7.0200174E-4	45.0	1
GTTAGAC	20	7.0200174E-4	45.0	18
TAAGTCC	20	7.0200174E-4	45.0	27
AGAGTAG	25	3.8798105E-5	45.0	1
GACCAAT	20	7.0200174E-4	45.0	8
AAGCCGG	25	3.8798105E-5	45.0	10
TGCGCGG	20	7.0200174E-4	45.0	2
CATAGTT	35	1.2055534E-7	45.0	36
GCTTACC	20	7.0200174E-4	45.0	25
GATATAC	20	7.0200174E-4	45.0	9
CGGGATA	25	3.8798105E-5	45.0	6
ATGACTA	20	7.0200174E-4	45.0	12
>>END_MODULE