##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936729.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	386362
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.081897288035574	31.0	31.0	33.0	28.0	34.0
2	31.132132559620253	31.0	31.0	34.0	28.0	34.0
3	30.874951470382697	31.0	30.0	34.0	27.0	34.0
4	34.84644193787174	35.0	35.0	37.0	32.0	37.0
5	35.12241628317484	35.0	35.0	37.0	33.0	37.0
6	35.112723300945746	36.0	35.0	37.0	32.0	37.0
7	31.150157106547745	35.0	35.0	37.0	0.0	37.0
8	33.13279773890807	35.0	35.0	37.0	17.0	37.0
9	36.405117480497566	39.0	35.0	39.0	32.0	39.0
10	36.13035443444231	37.0	35.0	39.0	32.0	39.0
11	36.3871265807714	38.0	35.0	39.0	32.0	39.0
12	36.395188967859156	38.0	35.0	39.0	32.0	39.0
13	36.30022103623027	38.0	35.0	39.0	32.0	39.0
14	37.39633556095061	39.0	36.0	40.0	32.0	41.0
15	37.60605080209751	39.0	36.0	41.0	33.0	41.0
16	37.55110492232673	39.0	36.0	41.0	33.0	41.0
17	37.456791817000635	39.0	36.0	41.0	32.0	41.0
18	37.36677002396716	39.0	36.0	40.0	32.0	41.0
19	37.13452151091463	39.0	36.0	40.0	32.0	41.0
20	37.061716731976745	39.0	35.0	40.0	32.0	41.0
21	37.08552083279412	39.0	35.0	40.0	32.0	41.0
22	37.15868278971534	39.0	35.0	40.0	32.0	41.0
23	37.233470165285404	39.0	35.0	40.0	32.0	41.0
24	37.14566391104715	39.0	35.0	40.0	32.0	41.0
25	36.83765484183227	39.0	35.0	40.0	31.0	41.0
26	36.8120881453145	39.0	35.0	40.0	31.0	41.0
27	36.82695244356329	39.0	35.0	40.0	31.0	41.0
28	36.83433671013195	39.0	35.0	40.0	31.0	41.0
29	36.84564993451737	39.0	35.0	40.0	31.0	41.0
30	36.624204761337815	38.0	35.0	40.0	31.0	41.0
31	36.560880728436025	38.0	35.0	40.0	31.0	41.0
32	36.35637821524891	38.0	35.0	40.0	30.0	41.0
33	36.17314073330193	38.0	35.0	40.0	30.0	41.0
34	36.06573369016622	38.0	35.0	40.0	30.0	41.0
35	35.966223386357875	39.0	35.0	40.0	29.0	41.0
36	35.85322314306272	38.0	35.0	40.0	29.0	41.0
37	35.777338351080076	38.0	35.0	40.0	29.0	41.0
38	35.664778109648466	38.0	35.0	40.0	28.0	41.0
39	35.630954907573724	38.0	35.0	40.0	27.0	41.0
40	35.510132984092635	38.0	35.0	40.0	27.0	41.0
41	35.36789591108857	38.0	35.0	40.0	26.0	41.0
42	35.3587904607596	38.0	35.0	40.0	26.0	41.0
43	35.263400644990966	38.0	34.0	40.0	26.0	41.0
44	35.07697703190272	38.0	34.0	40.0	25.0	41.0
45	35.01672783555318	38.0	34.0	40.0	25.0	41.0
46	34.912688100796665	38.0	34.0	40.0	24.0	41.0
47	34.854229970856345	38.0	34.0	40.0	24.0	41.0
48	34.790509936277374	38.0	34.0	40.0	24.0	41.0
49	34.68273018568079	37.0	34.0	40.0	24.0	41.0
50	34.54361453766157	37.0	34.0	40.0	24.0	41.0
51	32.86435260196396	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	15.0
9	25.0
10	24.0
11	22.0
12	18.0
13	14.0
14	13.0
15	29.0
16	59.0
17	117.0
18	201.0
19	375.0
20	609.0
21	921.0
22	1439.0
23	2039.0
24	3074.0
25	4377.0
26	5627.0
27	6206.0
28	6705.0
29	7314.0
30	8907.0
31	11092.0
32	14130.0
33	20053.0
34	30660.0
35	34717.0
36	38171.0
37	55865.0
38	74168.0
39	59370.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.67791345939818	21.6817388873647	24.345561934144662	13.294785719092458
2	34.9547833379059	24.222102587728607	24.84845818170524	15.97465589266025
3	31.567027813294267	23.83490094781578	29.466924801093274	15.131146437796678
4	28.47950885439044	25.37697806720123	28.33223764241825	17.811275435990083
5	25.573167133413744	29.518689726215314	26.45032378960664	18.45781935076431
6	24.880552435280904	36.119235328526095	25.555049409621027	13.445162826571972
7	67.63061584731417	17.50638002702129	11.502166362116357	3.3608377635481754
8	78.25277848235592	6.940382335736952	10.906352073961726	3.900487107945398
9	72.42353026436346	8.50161247741755	12.840807325772202	6.234049932446773
10	41.29236312059675	25.639167412944335	19.68697749778705	13.381491968671869
11	32.894021668797656	24.303891169421423	25.35653092177802	17.445556240002897
12	28.962734430404645	23.46322878543956	29.501607300925038	18.072429483230753
13	23.090262499935292	26.850725485425585	31.27145009084744	18.787561923791678
14	19.57283583789296	30.005538846988056	29.971373996407515	20.45025131871147
15	18.93069194175411	26.57171253901781	36.63093161335742	17.86666390587066
16	21.65300935392197	24.827493387030817	33.613295303368346	19.90620195567887
17	21.4935733845461	24.665469171398843	30.158763025349284	23.682194418705773
18	22.415247876343948	26.168463772317153	31.168955539105813	20.247332812233086
19	25.28664827286327	27.149409103379735	28.095671934610543	19.468270689146447
20	25.51493159265145	25.830438811270263	29.900714873615936	18.753914722462355
21	23.40214617379556	27.908541730294388	29.70607875515708	18.98323334075297
22	21.859551405158893	24.8194698236369	29.57278407296784	23.748194698236368
23	21.846868998503993	27.6062345675817	29.748008344505926	20.79888808940838
24	21.399878870075213	25.020317733110396	32.344019339376025	21.235784057438362
25	20.33973320357592	28.009483334282358	29.739984781112017	21.910798681029707
26	19.880060668492245	29.85774998576465	29.184029485301348	21.07815986044176
27	21.17418379654314	29.712549370797337	29.556736946180006	19.556529886479517
28	18.636925991686553	28.451814619450154	32.25886603755028	20.652393351313016
29	20.661711037834984	26.856937276440227	31.460909716794095	21.02044196893069
30	21.130183610189405	27.441622105693625	30.81954229453207	20.608651989584896
31	23.295251603418556	26.298134909747855	28.364073071368303	22.04254041546529
32	23.397487330534577	28.476920608134343	28.901392994135033	19.22419906719605
33	22.63498998348699	26.882302089750027	28.813133796801964	21.66957412996102
34	19.88704893338372	27.46543397124976	30.337869666271526	22.309647429094994
35	21.780609894347787	25.80455634870924	29.060828963510904	23.354004793432065
36	23.53052318809821	28.13190738219597	27.548516676070626	20.789052753635193
37	21.341384504687312	28.609438816446755	29.696243419383894	20.35293325948204
38	20.32187430440882	28.033812849089713	28.69355681977006	22.95075602673141
39	22.093270042084885	25.84441534105321	30.9932136183165	21.069100998545405
40	21.73738618187089	25.601896666856472	31.526651171699083	21.13406597957356
41	20.0239671603315	26.932772891744012	29.181182414419638	23.86207753350485
42	21.81503356955394	26.31780558129423	27.719858578224567	24.147302270927266
43	22.818237818418996	25.596978998969877	28.23259016155833	23.352193021052795
44	20.122320518063372	27.529622478401084	29.162547041375703	23.185509962159838
45	20.850911839156026	27.074867611204002	28.33042587003898	23.743794679600995
46	21.458373235463117	28.63635657751021	27.62331699287197	22.281953194154706
47	20.734958406882665	25.920768605608213	31.40810949316962	21.936163494339507
48	21.568891350598662	25.312271910798685	30.136762932172417	22.98207380643024
49	20.18314430508176	25.125400531108134	31.352203374037817	23.339251789772288
50	19.419094010280514	25.48387263757823	30.7286948509429	24.36833850119836
51	19.495706099461128	25.247824579021742	29.53214860674704	25.724320714770087
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	206.0
1	409.5
2	613.0
3	3353.0
4	6093.0
5	4207.0
6	2321.0
7	2127.0
8	1933.0
9	1881.5
10	1830.0
11	1782.5
12	1735.0
13	1675.5
14	1616.0
15	1641.5
16	1667.0
17	1536.0
18	1405.0
19	1443.5
20	1482.0
21	1737.0
22	1992.0
23	1967.5
24	1943.0
25	2538.5
26	3345.0
27	3556.0
28	4377.5
29	5199.0
30	6734.5
31	8270.0
32	9205.5
33	10141.0
34	11068.0
35	11995.0
36	12261.5
37	12528.0
38	13667.0
39	14806.0
40	16961.5
41	19117.0
42	21694.5
43	24272.0
44	26426.0
45	28580.0
46	32627.5
47	36675.0
48	35675.5
49	34676.0
50	32649.5
51	30623.0
52	26386.0
53	22149.0
54	20364.0
55	18579.0
56	17892.0
57	17205.0
58	15914.0
59	14623.0
60	13910.0
61	13197.0
62	12205.5
63	11214.0
64	9462.5
65	7711.0
66	6217.5
67	4724.0
68	4001.5
69	3279.0
70	2769.5
71	2260.0
72	1765.0
73	1270.0
74	1006.5
75	563.0
76	383.0
77	335.0
78	287.0
79	226.5
80	166.0
81	116.0
82	66.0
83	67.0
84	68.0
85	41.5
86	15.0
87	12.0
88	9.0
89	5.0
90	1.0
91	2.5
92	4.0
93	2.0
94	0.0
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	386362.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.15906024101209
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.19824089171638	27.14217602012019
2	8.744866205830643	6.149225554607809
3	3.207129379557119	3.3827896516970553
4	1.8340013967208166	2.5792706235763
5	1.2156732375712316	2.1370964296576567
6	0.9183042288842613	1.937202822175074
7	0.6876070701117983	1.6922932880144577
8	0.5304860342157566	1.4921112347205912
9	0.3911989091716016	1.2378767412404301
>10	4.925511897740536	41.183881924141645
>50	0.30056931078512644	6.276405453747364
>100	0.04272799025866993	2.6302780365964216
>500	7.366894872184471E-4	0.25590494339313286
>1k	0.0029467579488737885	1.903487276311876
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3435	0.889062588970965	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTATGC	1446	0.3742604086323189	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTATGCCG	1386	0.35873093109570814	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTATGCCGTCTTC	1082	0.28004824491021374	No Hit
GCTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTATGCCGTCTTCTGC	988	0.2557187301028569	No Hit
CTGTCTCTTATACACATCTGACGCAGCTTTCGTCGTATGCCGTCTTCTGCT	479	0.12397699566727577	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	448	0.11595343227336022	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.176492512203581E-4	0.0	0.0	0.14494179034170027	0.0
2	5.176492512203581E-4	0.0	0.0	0.6915793996303984	0.0
3	5.176492512203581E-4	0.0	0.0	0.8492035966269975	0.0
4	5.176492512203581E-4	0.0	0.0	1.1238165243993974	0.0
5	5.176492512203581E-4	0.0	0.0	2.0879382547973147	0.0
6	5.176492512203581E-4	0.0	0.0	2.5186224318126524	0.0
7	5.176492512203581E-4	0.0	0.0	2.983212634782924	0.0
8	5.176492512203581E-4	0.0	0.0	3.7889336943074112	0.0
9	5.176492512203581E-4	0.0	0.0	4.0332641408834204	0.0
10	5.176492512203581E-4	0.0	0.0	5.133527624352292	0.0
11	5.176492512203581E-4	0.0	0.0	6.161320212650312	0.0
12	5.176492512203581E-4	0.0	0.0	7.364854721737645	0.0
13	5.176492512203581E-4	0.0	0.0	7.717373861818709	0.0
14	5.176492512203581E-4	0.0	0.0	7.865421547667731	0.0
15	5.176492512203581E-4	0.0	0.0	8.184293486419472	0.0
16	5.176492512203581E-4	0.0	0.0	8.826955031809547	0.0
17	5.176492512203581E-4	0.0	0.0	9.578322919955896	0.0
18	5.176492512203581E-4	0.0	0.0	10.4073381957853	0.0
19	5.176492512203581E-4	0.0	0.0	10.892634368804385	0.0
20	5.176492512203581E-4	0.0	0.0	11.373012873936878	0.0
21	5.176492512203581E-4	0.0	0.0	11.942168225653662	0.0
22	5.176492512203581E-4	0.0	0.0	12.550406095837582	0.0
23	5.176492512203581E-4	0.0	0.0	13.205491223256946	0.0
24	5.176492512203581E-4	0.0	0.0	13.684575605261386	0.0
25	5.176492512203581E-4	0.0	0.0	14.101800901744996	0.0
26	5.176492512203581E-4	0.0	0.0	14.4985790528054	0.0
27	5.176492512203581E-4	0.0	0.0	14.943239759603689	0.0
28	5.176492512203581E-4	0.0	0.0	15.360982705338516	0.0
29	5.176492512203581E-4	0.0	0.0	15.795549251738008	0.0
30	5.176492512203581E-4	0.0	0.0	16.322775014105943	0.0
31	5.176492512203581E-4	0.0	0.0	16.819718295277486	0.0
32	5.176492512203581E-4	0.0	0.0	17.310967434685605	0.0
33	5.176492512203581E-4	0.0	0.0	17.732075100553367	0.0
34	5.176492512203581E-4	0.0	0.0	18.148523923160145	0.0
35	5.176492512203581E-4	0.0	0.0	18.60017289484991	0.0
36	5.176492512203581E-4	0.0	0.0	19.058551306805533	0.0
37	5.176492512203581E-4	0.0	0.0	19.550835744716096	0.0
38	5.176492512203581E-4	0.0	0.0	19.996014100765603	0.0
39	5.176492512203581E-4	0.0	0.0	20.489075012552995	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	20	7.0274656E-4	45.000004	25
TAGACCG	35	1.2091368E-7	45.000004	1
AGAACCG	20	7.0274656E-4	45.000004	10
AAGTTAG	30	2.161747E-6	45.000004	1
ACGTGCG	20	7.0274656E-4	45.000004	1
CGAATAT	60	0.0	45.000004	14
AACGACG	20	7.0274656E-4	45.000004	1
GCTACGA	60	0.0	45.000004	10
GCGCTAG	20	7.0274656E-4	45.000004	9
CCCGGTC	20	7.0274656E-4	45.000004	36
CGATCAC	20	7.0274656E-4	45.000004	13
TCGCGAG	20	7.0274656E-4	45.000004	15
TAGTAAC	20	7.0274656E-4	45.000004	10
TGATCGG	45	3.8380676E-10	45.0	2
GCGAATT	25	3.885982E-5	45.0	9
ACCGCAC	25	3.885982E-5	45.0	10
GGCGCTA	25	3.885982E-5	45.0	8
GGTTCGC	25	3.885982E-5	45.0	41
CGTTTTT	1570	0.0	43.71019	1
TACGAAT	65	0.0	41.53846	12
>>END_MODULE