FastQCFastQC Report
Sat 14 Jan 2017
SRR2936726.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2936726.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences773470
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT47250.6108834214643102No Hit
GAATCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTC16060.20763571954956236No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCG13370.1728573829624937No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGC13080.1691080455609138No Hit
GCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCTGC11020.14247482126003594No Hit
CCTGTCTCTTATACACATCTGACGCTCCTTGGTTCGTATGCCGTCTTCTGC8390.1084722096526045No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGGTA207.0318533E-445.0000045
GTGTCGA207.0318533E-445.00000421
TATAGCG207.0318533E-445.0000041
TCACGAC551.8189894E-1245.025
CGGTCTA551.8189894E-1245.031
CGTTTTT21850.040.3661351
TACGTAG451.9268555E-840.01
CACGGTT356.24564E-638.57142637
GCTACGA1300.038.0769210
ACGGTCT659.094947E-1238.0769230
CACTCGC301.13957685E-437.49999622
CGACTAG301.13957685E-437.4999962
GACGTAG552.746674E-936.818181
CGTTAGG800.036.5625042
AACCGAT250.002106683336.010
GTACCGG250.002106683336.02
GATCGAT250.002106683336.031
CAACCCG250.002106683336.018
GTTAGCG250.002106683336.01
CGATTTC250.002106683336.010
Produced by FastQC (version 0.11.5)