##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936724.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	167598
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.96242198594255	31.0	30.0	33.0	28.0	34.0
2	31.047255933841694	31.0	31.0	34.0	28.0	34.0
3	30.695628826119645	31.0	30.0	34.0	27.0	34.0
4	34.627710354538834	35.0	35.0	37.0	32.0	37.0
5	35.00414086086946	35.0	35.0	37.0	32.0	37.0
6	35.042178307617036	35.0	35.0	37.0	32.0	37.0
7	31.39636511175551	35.0	35.0	37.0	0.0	37.0
8	33.26436472988938	35.0	35.0	37.0	17.0	37.0
9	36.33657322879748	39.0	35.0	39.0	32.0	39.0
10	36.1494230241411	37.0	35.0	39.0	32.0	39.0
11	36.31685938973019	37.0	35.0	39.0	32.0	39.0
12	36.252693946228476	37.0	35.0	39.0	32.0	39.0
13	35.89834604231554	38.0	35.0	39.0	31.0	39.0
14	36.96377045072137	39.0	35.0	40.0	31.0	41.0
15	37.258988770749056	39.0	35.0	40.0	32.0	41.0
16	37.28245563789544	39.0	35.0	40.0	32.0	41.0
17	37.20446544708171	39.0	35.0	40.0	32.0	41.0
18	37.05902218403561	39.0	35.0	40.0	32.0	41.0
19	36.69914915452452	38.0	35.0	40.0	31.0	41.0
20	36.57063926777169	38.0	35.0	40.0	31.0	41.0
21	36.56002458263225	38.0	35.0	40.0	31.0	41.0
22	36.621105263785964	38.0	35.0	40.0	31.0	41.0
23	36.73604100287593	38.0	35.0	40.0	32.0	41.0
24	36.60768624923925	38.0	35.0	40.0	31.0	41.0
25	36.3534827384575	38.0	35.0	40.0	31.0	41.0
26	36.27441258248905	38.0	35.0	40.0	31.0	41.0
27	36.27613694674161	38.0	35.0	40.0	31.0	41.0
28	36.320158951777465	38.0	35.0	40.0	31.0	41.0
29	36.29914438119787	38.0	35.0	40.0	31.0	41.0
30	36.0301793577489	38.0	35.0	40.0	30.0	41.0
31	35.905696965357585	38.0	35.0	40.0	30.0	41.0
32	35.481682358978034	38.0	34.0	40.0	29.0	41.0
33	35.16043150872922	38.0	34.0	40.0	26.0	41.0
34	34.934342891919954	38.0	34.0	40.0	24.0	41.0
35	34.671117793768424	38.0	34.0	40.0	23.0	41.0
36	34.609512046683136	38.0	34.0	40.0	23.0	41.0
37	34.40683659709543	38.0	34.0	40.0	22.0	41.0
38	34.34855427869068	38.0	34.0	40.0	22.0	41.0
39	34.34540984975954	38.0	34.0	40.0	22.0	41.0
40	34.15081325552811	37.0	33.0	40.0	21.0	41.0
41	34.063312211362906	37.0	33.0	40.0	20.0	41.0
42	33.9900297139584	37.0	33.0	40.0	20.0	41.0
43	33.86323225814151	37.0	33.0	40.0	19.0	41.0
44	33.61434503991694	37.0	33.0	40.0	18.0	41.0
45	33.58611677943651	37.0	33.0	40.0	18.0	41.0
46	33.47035167484099	37.0	33.0	40.0	18.0	41.0
47	33.36270122555162	36.0	32.0	40.0	18.0	41.0
48	33.30502153963651	36.0	32.0	40.0	18.0	41.0
49	33.21459683289777	36.0	32.0	40.0	18.0	41.0
50	32.97412260289502	36.0	32.0	40.0	15.0	41.0
51	31.320415518084943	35.0	29.0	38.0	11.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	8.0
10	6.0
11	9.0
12	8.0
13	8.0
14	4.0
15	9.0
16	34.0
17	47.0
18	117.0
19	242.0
20	349.0
21	615.0
22	838.0
23	1294.0
24	2052.0
25	2940.0
26	3886.0
27	4043.0
28	3922.0
29	3980.0
30	4395.0
31	5369.0
32	6928.0
33	9458.0
34	15371.0
35	16756.0
36	14964.0
37	21640.0
38	28061.0
39	20240.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.22660174942422	19.043783338703328	23.32724734185372	18.402367570018736
2	36.86619172066493	23.873793243356126	23.379157269179824	15.880857766799124
3	29.429348798911683	22.141075669160728	33.73488943782145	14.694686094106135
4	25.256864640389505	22.80874473442404	34.53621164930369	17.398178975882768
5	24.907815129058818	26.221673289657392	32.089881740832226	16.780629840451557
6	23.720450124703156	31.27781954438597	31.289156195181327	13.712574135729543
7	62.050859795462955	16.257353906371197	17.91787491497512	3.7739113831907303
8	71.36958674924522	6.334801131278415	17.136839341758257	5.158772777718111
9	67.22037255814509	5.926681702645617	18.96741011229251	7.885535626916789
10	40.79702621749663	21.22698361555627	25.676320719817657	12.29966944712944
11	32.53439778517643	21.47340660389742	30.830320170885095	15.161875440041051
12	29.375052208260243	20.70848100812659	33.48011312784162	16.436353655771548
13	19.811095597799497	28.798673015191113	36.60365875487774	14.786572632131648
14	16.948292939056554	30.740820296184918	33.867349252377714	18.443537512380818
15	14.634422845141351	24.333225933483693	46.001145598396164	15.031205622978794
16	16.127280755140276	22.010405852098476	41.95217126696023	19.910142125801023
17	16.491843578085657	21.687012971515173	36.45687896036945	25.364264490029715
18	20.458478024797433	22.80993806608671	37.69436389455721	19.037220014558645
19	20.48115132638814	26.53134285612	33.00815045525603	19.979355362235825
20	23.638110239978996	24.207926108903447	35.125717490662176	17.028246160455378
21	19.965632048115133	26.326686475972267	35.222974021169705	18.484707454742896
22	17.639828637573242	24.35530256924307	35.45686702705283	22.54800176613086
23	16.97693289896061	26.39351304908173	34.84826787909164	21.781286172866025
24	20.56289454528097	21.818279454408763	36.992088211076506	20.62673778923376
25	16.567023472833807	25.805797205217246	35.706870010381984	21.920309311566964
26	17.846275015214978	27.970500841298822	34.36675855320469	19.816465590281506
27	17.29376245539923	29.07134930011098	34.330958603324625	19.30392964116517
28	14.612346209381974	25.268201291184862	40.61683313643361	19.50261936299956
29	18.700103819854654	25.142901466604613	36.00162293106123	20.155371782479506
30	18.243654458883757	26.742562560412413	36.258785904366405	18.754997076337425
31	23.33500399766107	25.093974868435183	30.44368071218034	21.12734042172341
32	22.269358822897647	29.70202508383155	30.951443334646	17.077172758624805
33	20.858840797622882	27.292091791071492	31.097029797491615	20.752037613814007
34	21.43760665401735	25.994940273750284	31.635222377355337	20.932230694877028
35	18.14818792587024	29.934724758052006	29.99737467034213	21.91971264573563
36	24.33143593598969	25.95078700223153	30.9186267139226	18.79915034785618
37	18.91848351412308	30.6781703838948	32.44788123963293	17.955464862349192
38	18.075991360278763	29.672191792264822	29.506915356985168	22.744901490471246
39	22.320075418561082	26.15484671654793	31.988448549505367	19.536629315385625
40	22.148235659136745	25.76403059702383	33.00337712860536	19.08435661523407
41	21.01755390875786	26.509266220360622	29.66682179978281	22.8063580710987
42	21.72818291387725	26.974665568801537	29.722311722096922	21.57483979522429
43	23.676893519015742	24.96927170968627	29.416222150622325	21.937612620675665
44	19.364192890129953	27.100562059213118	30.654303750641414	22.880941300015515
45	19.76694232628074	28.528383393596584	28.027184095275597	23.677490184847073
46	23.48178379216936	26.188260003102666	30.06539457511426	20.26456162961372
47	19.3373429277199	26.170956693993965	33.87629923984773	20.615401138438404
48	21.21624363059225	25.76104726786716	30.372677478251532	22.65003162328906
49	19.506199357987565	23.48476712132603	34.555304955906394	22.45372856478001
50	19.7454623563527	25.096958197591857	32.43654458883757	22.721034857217866
51	18.917290182460412	25.616654136684208	29.434718791393692	26.031336889461688
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	126.0
1	171.0
2	216.0
3	2547.5
4	4879.0
5	3365.5
6	1852.0
7	1720.0
8	1588.0
9	1622.5
10	1657.0
11	1587.0
12	1517.0
13	1426.0
14	1335.0
15	1232.0
16	1129.0
17	1074.5
18	1020.0
19	974.5
20	929.0
21	919.0
22	909.0
23	961.0
24	1013.0
25	980.0
26	1203.5
27	1460.0
28	1720.0
29	1980.0
30	2352.5
31	2725.0
32	3127.5
33	3530.0
34	3943.5
35	4357.0
36	4569.5
37	4782.0
38	5402.5
39	6023.0
40	7021.5
41	8020.0
42	9853.5
43	11687.0
44	13370.5
45	15054.0
46	17785.5
47	20517.0
48	18827.0
49	17137.0
50	16193.0
51	15249.0
52	12549.0
53	9849.0
54	8128.5
55	6408.0
56	5780.0
57	5152.0
58	4856.0
59	4560.0
60	4013.5
61	3467.0
62	3239.0
63	3011.0
64	2317.5
65	1624.0
66	1207.5
67	791.0
68	645.0
69	499.0
70	366.5
71	234.0
72	205.0
73	176.0
74	131.0
75	74.0
76	62.0
77	39.0
78	16.0
79	12.5
80	9.0
81	8.0
82	7.0
83	6.0
84	5.0
85	2.5
86	0.0
87	0.5
88	1.0
89	2.0
90	3.0
91	1.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	167598.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.16930989629948
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.90516015069329	29.354168904163537
2	9.571524597083053	7.306769770522322
3	3.6516546560160075	4.1814341459921955
4	1.9071141611042504	2.911729256912374
5	1.1958543715120915	2.282246804854473
6	0.7862937893733098	1.8007374789675294
7	0.6237201231808164	1.6664876669172664
8	0.45489362367322694	1.3890380553467225
9	0.32671054071376093	1.1223284287401998
>10	4.417626737115255	38.56430267664292
>50	0.12661987463069205	3.0054057924318904
>100	0.025011333260383614	1.9928638766572395
>500	0.0031264166575479518	1.1420184011742383
>1k	0.004689624986321928	3.2804687406770965
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2882	1.719590925906037	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGC	1332	0.7947588873375577	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCG	1284	0.7661189274335016	No Hit
GCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTCTGC	996	0.5942791680091648	No Hit
GAATCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTC	918	0.5477392331650736	No Hit
CCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTCTGC	469	0.2798362748958818	No Hit
CTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTCTGCT	303	0.18078974689435434	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCT	273	0.16288977195431925	No Hit
TCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTCTGC	254	0.15155312115896372	No Hit
TATCGCGGGGAATGTTGTACTTACACCTCTGTACAGAAATGACAGTGAGCT	244	0.14558646284561869	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTC	220	0.1312664828935906	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	206	0.12291316125490757	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGACATGAGTCGTA	192	0.11455983961622454	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	181	0.10799651547154501	No Hit
ACTGTCTCTTATACACATCTGACGCGACATGAGTCGTATGCCGTCTTCTGC	172	0.1026265229895345	No Hit
TATTAGATGTTTGTGTAATCACTTGCTTATTTAAATAAATTTTGAGTACTG	168	0.10023985966419646	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.22315302091910405	0.0
2	0.0	0.0	0.0	1.574601128891753	0.0
3	0.0	0.0	0.0	1.9003806728003914	0.0
4	0.0	0.0	0.0	2.48451652167687	0.0
5	0.0	0.0	0.0	4.3097172997291135	0.0
6	0.0	0.0	0.0	4.952923065907708	0.0
7	0.0	0.0	0.0	5.7834819031253355	0.0
8	0.0	0.0	0.0	7.108080048687932	0.0
9	0.0	0.0	0.0	7.500686165706035	0.0
10	5.966658313345028E-4	0.0	0.0	9.331853602071623	0.0
11	0.0011933316626690056	0.0	0.0	10.679721715056266	0.0
12	0.0011933316626690056	0.0	0.0	12.648718958460124	0.0
13	0.0011933316626690056	0.0	0.0	13.15409491760045	0.0
14	0.0011933316626690056	0.0	0.0	13.387987923483573	0.0
15	0.0011933316626690056	0.0	0.0	13.856967266912493	0.0
16	0.0011933316626690056	0.0	0.0	14.662466139214072	0.0
17	0.0011933316626690056	0.0	0.0	15.638611439277318	0.0
18	0.0011933316626690056	0.0	0.0	16.723349920643443	0.0
19	0.0011933316626690056	0.0	0.0	17.31882242031528	0.0
20	0.0011933316626690056	0.0	0.0	17.871334980131028	0.0
21	0.0011933316626690056	0.0	0.0	18.56466067614172	0.0
22	0.0011933316626690056	0.0	0.0	19.31466962612919	0.0
23	0.0011933316626690056	0.0	0.0	20.040215277031944	0.0
24	0.0011933316626690056	0.0	0.0	20.547381233666272	0.0
25	0.0011933316626690056	0.0	0.0	21.008603921287843	0.0
26	0.0011933316626690056	0.0	0.0	21.43999331734269	0.0
27	0.0011933316626690056	0.0	0.0	21.867206052578194	0.0
28	0.0011933316626690056	0.0	0.0	22.32663874270576	0.0
29	0.0011933316626690056	0.0	0.0	22.783088103676654	0.0
30	0.0011933316626690056	0.0	0.0	23.323667346865715	0.0
31	0.0011933316626690056	0.0	0.0	23.847539946777406	0.0
32	0.0011933316626690056	0.0	0.0	24.31293929521832	0.0
33	0.0011933316626690056	0.0	0.0	24.828458573491332	0.0
34	0.0011933316626690056	0.0	0.0	25.29445458776358	0.0
35	0.0011933316626690056	0.0	0.0	25.79147722526522	0.0
36	0.0011933316626690056	0.0	0.0	26.22644661630807	0.0
37	0.0011933316626690056	0.0	0.0	26.660222675688253	0.0
38	0.0017899974940035084	0.0	0.0	27.136362009093187	0.0
39	0.002386663325338011	0.0	0.0	27.620854664136804	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGCGTGG	30	2.1543747E-6	45.000004	2
AATGGAG	30	2.1543747E-6	45.000004	17
GACCCCA	30	2.1543747E-6	45.000004	29
ACGGGAG	30	2.1543747E-6	45.000004	5
CCGAGGG	30	2.1543747E-6	45.000004	3
GGGCCGA	30	2.1543747E-6	45.000004	7
GGACCAT	30	2.1543747E-6	45.000004	8
TCTGCGG	20	7.016028E-4	45.0	4
ACAACGG	20	7.016028E-4	45.0	2
TTCTAGA	25	3.8765058E-5	45.0	44
TGTCAGT	20	7.016028E-4	45.0	31
TTAGGCG	20	7.016028E-4	45.0	1
TAGAGCC	20	7.016028E-4	45.0	41
AACCGAG	20	7.016028E-4	45.0	11
CAAACTC	25	3.8765058E-5	45.0	19
ATTAGGA	25	3.8765058E-5	45.0	4
ATCTACC	20	7.016028E-4	45.0	15
GGATGCA	25	3.8765058E-5	45.0	9
CATGCAT	20	7.016028E-4	45.0	25
GCGCGAT	20	7.016028E-4	45.0	1
>>END_MODULE