##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936723.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	465353
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.0812028717984	31.0	31.0	33.0	28.0	34.0
2	31.119438361845738	31.0	31.0	34.0	28.0	34.0
3	30.879970688917876	31.0	31.0	34.0	27.0	34.0
4	34.86728999275819	35.0	35.0	37.0	32.0	37.0
5	35.130008832004954	35.0	35.0	37.0	33.0	37.0
6	35.11159485379916	36.0	35.0	37.0	32.0	37.0
7	31.095286803781214	35.0	35.0	37.0	0.0	37.0
8	33.090608634735354	35.0	35.0	37.0	17.0	37.0
9	36.28403598988295	39.0	35.0	39.0	32.0	39.0
10	36.074126523305964	37.0	35.0	39.0	32.0	39.0
11	36.34961201496498	37.0	35.0	39.0	32.0	39.0
12	36.395508356022205	38.0	35.0	39.0	32.0	39.0
13	36.308383098422055	38.0	35.0	39.0	32.0	39.0
14	37.34767155256332	39.0	36.0	40.0	32.0	41.0
15	37.57609384703655	39.0	36.0	41.0	33.0	41.0
16	37.51452123441774	39.0	36.0	41.0	33.0	41.0
17	37.43921711045164	39.0	36.0	40.0	32.0	41.0
18	37.35299224459711	39.0	36.0	40.0	32.0	41.0
19	37.13943178619242	39.0	36.0	40.0	32.0	41.0
20	37.14131852593623	39.0	35.0	40.0	32.0	41.0
21	37.15388318115495	39.0	35.0	40.0	32.0	41.0
22	37.211476019279985	39.0	35.0	40.0	32.0	41.0
23	37.24102563000561	39.0	35.0	40.0	32.0	41.0
24	37.15286245065574	39.0	35.0	40.0	32.0	41.0
25	36.83705273201204	39.0	35.0	40.0	31.0	41.0
26	36.8210799113791	39.0	35.0	40.0	31.0	41.0
27	36.84973342817173	39.0	35.0	40.0	31.0	41.0
28	36.862005832131736	39.0	35.0	40.0	31.0	41.0
29	36.88123209692427	39.0	35.0	40.0	31.0	41.0
30	36.667690978676404	39.0	35.0	40.0	31.0	41.0
31	36.65457835234757	39.0	35.0	40.0	31.0	41.0
32	36.5123400945089	39.0	35.0	40.0	30.0	41.0
33	36.363792647731934	39.0	35.0	40.0	30.0	41.0
34	36.3291157465408	39.0	35.0	40.0	30.0	41.0
35	36.27598188901759	39.0	35.0	40.0	30.0	41.0
36	36.17440308754859	39.0	35.0	40.0	30.0	41.0
37	36.09755175103631	38.0	35.0	40.0	30.0	41.0
38	36.01525938373665	38.0	35.0	40.0	29.0	41.0
39	35.943511699720425	38.0	35.0	40.0	29.0	41.0
40	35.835864386820326	38.0	35.0	40.0	29.0	41.0
41	35.69909509555112	38.0	35.0	40.0	28.0	41.0
42	35.72430176661588	38.0	35.0	40.0	28.0	41.0
43	35.68212948020105	38.0	35.0	40.0	28.0	41.0
44	35.485173620885654	38.0	34.0	40.0	27.0	41.0
45	35.40277380827028	38.0	34.0	40.0	27.0	41.0
46	35.2779911164213	38.0	34.0	40.0	27.0	41.0
47	35.240507743583905	38.0	34.0	40.0	26.0	41.0
48	35.197551106364415	38.0	34.0	40.0	27.0	41.0
49	35.08863378983266	38.0	34.0	40.0	26.0	41.0
50	34.941637853414505	37.0	34.0	40.0	26.0	41.0
51	33.31312573465735	36.0	32.0	39.0	22.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	18.0
9	50.0
10	53.0
11	28.0
12	19.0
13	21.0
14	20.0
15	27.0
16	62.0
17	99.0
18	231.0
19	368.0
20	704.0
21	1032.0
22	1493.0
23	2174.0
24	3109.0
25	4241.0
26	5552.0
27	6569.0
28	7381.0
29	8739.0
30	10590.0
31	13356.0
32	17526.0
33	24285.0
34	37244.0
35	41427.0
36	46473.0
37	67715.0
38	91606.0
39	73135.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.60669427294978	22.309945353312433	23.65215223711892	12.431208136618869
2	33.47093496764821	24.43607326051406	24.54545259190335	17.547539179934372
3	32.7708212905042	23.854149430647272	27.83198990873595	15.543039370112583
4	29.228134341027136	25.029601184477162	27.156803544835856	18.58546092965985
5	25.51331999578814	29.719589215068993	25.523849636727387	19.24324115241548
6	26.430043429396612	35.33876433589125	24.753681613742685	13.477510620969458
7	68.09239437588239	18.105180368451474	10.01390342385243	3.7885218318136986
8	78.28551658633339	7.304132561732706	9.721007493236318	4.689343358697591
9	72.06056477555748	8.25975119962695	12.411653089160273	7.268030935655298
10	40.52901775641287	26.523305963429912	18.858801812817365	14.08887446733985
11	32.263249619106354	25.46325047866888	24.367952930356097	17.905546971868667
12	30.43216654883497	21.953656686429444	29.56723175739707	18.046945007338515
13	24.63506198520263	24.88992227405862	30.382526812978533	20.092488927760215
14	19.150408399645	29.276054951832265	29.36996215775981	22.203574490762925
15	19.044252427726907	26.457334539586082	35.86030389833094	18.63810913435607
16	20.806785386577502	24.338512913852494	34.60942553287504	20.24527616669496
17	20.546337941304774	25.14607190670308	29.60827586799698	24.69931428399516
18	21.84750071451135	26.099541638283196	30.864096717975386	21.188860929230067
19	24.463579261334946	26.87078411442496	27.465171600913713	21.20046502332638
20	26.22804623586826	24.780542942669328	29.152492838769707	19.83891798269271
21	24.763566582787693	26.851658848229192	28.385118393993373	19.999656174989738
22	22.33508755718777	25.675992203767894	27.98133889756808	24.007581341476257
23	22.52247218778003	26.85617155148887	29.07040461756989	21.550951643161213
24	23.01285260866482	24.375259211824144	30.40057762601724	22.2113105534938
25	22.113105534937993	26.702524750028473	28.50094444432506	22.683425270708472
26	20.52291486248074	28.383829050204902	28.637185104640995	22.456070982673367
27	21.972996843256627	28.369431377900217	28.82005703197358	20.83751474686958
28	18.96173442526426	28.14057285544522	30.954995455063145	21.94269726422737
29	19.991920112258864	26.515999681961866	30.363831328045592	23.12824887773368
30	21.058852097225117	28.088354432011826	28.81747834439662	22.035315126366438
31	22.554705782492	27.282729454843956	26.222673970082926	23.939890792581117
32	22.603485955822784	28.10167765115944	27.20429437437816	22.090542018639614
33	21.18692691354735	29.087166086820115	26.756032517250343	22.969874482382192
34	20.553429332141405	27.397695942649985	28.384473722099134	23.664401003109468
35	20.747475572307476	25.716391642473564	27.668887919493372	25.867244865725592
36	21.05562873775392	28.648359417474477	25.940952352300293	24.355059492471305
37	20.615102943356977	29.158079995186448	28.25081174936016	21.976005312096408
38	20.69998474276517	28.243935249154944	26.65460413922334	24.401475868856544
39	21.492286500785426	26.89313274009193	28.00905978902038	23.60552097010227
40	21.662694771496046	26.879164849050074	28.95157009839842	22.506570281055456
41	18.077674367630596	27.491603148577532	28.679733449660795	25.750989034131077
42	20.525063768794872	26.810399846997868	26.969418914243597	25.695117469963662
43	21.769925196571204	26.264577643208487	27.5614426037868	24.4040545564335
44	20.676561663941136	26.938044882057277	27.965007209580683	24.420386244420904
45	20.320917668952386	27.037754135032976	27.100717090037023	25.54061110597761
46	21.15899113146364	28.688758856180147	26.84929505128365	23.30295496107256
47	20.236035869544196	26.74249440747132	30.116707101920476	22.90476262106401
48	20.382806170799373	24.950736322748536	28.57250302458564	26.093954481866454
49	19.249043199463635	25.55952148154197	30.587317584715258	24.60411773427914
50	18.86116560976291	26.027338386128378	29.5545532101437	25.55694279396501
51	18.839676546621597	26.3531125833507	26.94449160099967	27.86271926902803
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	280.0
1	537.0
2	794.0
3	2903.0
4	5012.0
5	3416.5
6	1821.0
7	1683.5
8	1546.0
9	1518.0
10	1490.0
11	1408.0
12	1326.0
13	1318.0
14	1310.0
15	1301.5
16	1293.0
17	1261.5
18	1230.0
19	1313.0
20	1396.0
21	1539.0
22	1682.0
23	2011.0
24	2340.0
25	3039.0
26	4042.0
27	4346.0
28	5608.0
29	6870.0
30	7512.5
31	8155.0
32	9985.5
33	11816.0
34	13235.5
35	14655.0
36	15406.5
37	16158.0
38	17513.5
39	18869.0
40	20416.0
41	21963.0
42	25242.0
43	28521.0
44	31093.0
45	33665.0
46	38049.5
47	42434.0
48	41474.0
49	40514.0
50	38596.0
51	36678.0
52	32466.0
53	28254.0
54	25100.0
55	21946.0
56	20991.5
57	20037.0
58	19611.0
59	19185.0
60	19615.0
61	20045.0
62	18322.5
63	16600.0
64	13760.0
65	10920.0
66	8807.0
67	6694.0
68	5504.5
69	4315.0
70	3529.0
71	2743.0
72	2397.5
73	2052.0
74	1596.0
75	892.5
76	645.0
77	592.0
78	539.0
79	361.5
80	184.0
81	131.0
82	78.0
83	57.0
84	36.0
85	27.5
86	19.0
87	15.0
88	11.0
89	7.5
90	4.0
91	2.5
92	1.0
93	1.5
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	465353.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	31.625306160674487
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.18579867961056	23.461485960168453
2	9.4370270242287	5.968977377755831
3	3.649039545492241	3.4620597845580185
4	2.066251577242098	2.6138335494103164
5	1.3965007689479236	2.2082382185797718
6	0.9788330794271827	1.8573537490248277
7	0.7807062463735274	1.728305184417964
8	0.5580164815101082	1.4117953656367621
9	0.46390272167003926	1.3203959041426638
>10	6.159561353048953	46.05443704869392
>50	0.27472213641354576	5.221505154087258
>100	0.04420034372990217	2.4174192519396573
>500	0.0020400158644570234	0.36774191983069243
>1k	0.003400026440761705	1.9064515317538526
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2880	0.6188850184698498	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCG	1609	0.3457590259437459	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGC	1581	0.33974208826417795	No Hit
GCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTGC	1421	0.3053595872380752	Illumina PCR Primer Index 8 (95% over 22bp)
GAATCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTC	1374	0.2952597275616575	RNA PCR Primer, Index 29 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTGC	694	0.14913409820072074	RNA PCR Primer, Index 29 (96% over 25bp)
CTGTCTCTTATACACATCTGACGCCACTAACCTCGTATGCCGTCTTCTGCT	514	0.11045378454635513	RNA PCR Primer, Index 29 (96% over 26bp)
GGTATGAATGGTCACATTGCTTGGGGAAAGTAAGTTGAGAACATTCTCAAG	502	0.10787509696939743	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	478	0.10271772181548201	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.13000883200495109	0.0
2	0.0	0.0	0.0	0.8198077588411378	0.0
3	0.0	0.0	0.0	1.0456578124563503	0.0
4	0.0	0.0	0.0	1.3886232601917254	0.0
5	0.0	0.0	0.0	2.533130763098121	0.0
6	0.0	0.0	0.0	3.029743012293893	0.0
7	0.0	0.0	0.0	3.539893371268693	0.0
8	0.0	0.0	0.0	4.464567758239444	0.0
9	0.0	0.0	0.0	4.76176150148382	0.0
10	0.0	0.0	0.0	5.78893871963864	0.0
11	2.1489063141314228E-4	0.0	0.0	6.73402771659364	0.0
12	2.1489063141314228E-4	0.0	0.0	7.824597671015336	0.0
13	2.1489063141314228E-4	0.0	0.0	8.181531009792566	0.0
14	2.1489063141314228E-4	0.0	0.0	8.339045842618399	0.0
15	2.1489063141314228E-4	0.0	0.0	8.650637258167455	0.0
16	2.1489063141314228E-4	0.0	0.0	9.203550852793471	0.0
17	2.1489063141314228E-4	0.0	0.0	9.854024794081052	0.0
18	2.1489063141314228E-4	0.0	0.0	10.562519205850183	0.0
19	2.1489063141314228E-4	0.0	0.0	10.994449374990598	0.0
20	2.1489063141314228E-4	0.0	0.0	11.423585965922644	0.0
21	2.1489063141314228E-4	0.0	0.0	11.943406403311036	0.0
22	2.1489063141314228E-4	0.0	0.0	12.508138982664773	0.0
23	2.1489063141314228E-4	0.0	0.0	13.038489060992408	0.0
24	2.1489063141314228E-4	0.0	0.0	13.476006386549566	0.0
25	2.1489063141314228E-4	0.0	0.0	13.84626294447441	0.0
26	2.1489063141314228E-4	0.0	0.0	14.20598986146001	0.0
27	2.1489063141314228E-4	0.0	0.0	14.569584809811047	0.0
28	2.1489063141314228E-4	0.0	0.0	14.942849836575675	0.0
29	2.1489063141314228E-4	0.0	0.0	15.310312816292148	0.0
30	2.1489063141314228E-4	0.0	0.0	15.736011157121583	0.0
31	2.1489063141314228E-4	0.0	0.0	16.167726435630584	0.0
32	4.2978126282628456E-4	0.0	0.0	16.57215060395012	0.0
33	4.2978126282628456E-4	0.0	0.0	16.959168631125188	0.0
34	4.2978126282628456E-4	0.0	0.0	17.37283309659549	0.0
35	4.2978126282628456E-4	0.0	0.0	17.79702720300503	0.0
36	4.2978126282628456E-4	0.0	0.0	18.207683199635547	0.0
37	4.2978126282628456E-4	0.0	0.0	18.60737977406399	0.0
38	4.2978126282628456E-4	0.0	0.0	19.011159270489284	0.0
39	4.2978126282628456E-4	0.0	0.0	19.388722109882174	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATAGCG	30	2.1627056E-6	45.000004	1
ACGCGAA	25	3.887216E-5	45.0	22
GATCGTC	20	7.028956E-4	45.0	41
CGCGGTC	20	7.028956E-4	45.0	31
GACCGGA	20	7.028956E-4	45.0	9
CGACCGT	20	7.028956E-4	45.0	21
CCTCGAG	20	7.028956E-4	45.0	28
CCGTCAA	20	7.028956E-4	45.0	18
CGTTACG	25	3.887216E-5	45.0	25
GTTACGA	25	3.887216E-5	45.0	26
ACGATCA	25	3.887216E-5	45.0	29
TACGATC	25	3.887216E-5	45.0	28
TACGACG	25	3.887216E-5	45.0	1
CCTCATA	20	7.028956E-4	45.0	44
TCGTCTC	20	7.028956E-4	45.0	42
GTCCGAA	20	7.028956E-4	45.0	38
ACTAAAC	20	7.028956E-4	45.0	27
AGTTACG	35	1.2098462E-7	45.0	1
TTACGGA	20	7.028956E-4	45.0	40
TTACGAT	25	3.887216E-5	45.0	27
>>END_MODULE