SRR2936721.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2936721.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	243643
Sequences flagged as poor quality	0
Sequence length	51
%GC	43

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4070	1.670476886263919	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCG	3685	1.512458802428143	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGC	3567	1.464027285823931	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTC	2988	1.2263845051981792	No Hit
GCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGC	2539	1.042098480153339	Illumina PCR Primer Index 11 (95% over 23bp)
CCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGC	910	0.3734972890663799	Illumina PCR Primer Index 11 (95% over 23bp)
GAATGACTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCT	875	0.35913200871767303	No Hit
CTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGCT	766	0.3143944213459857	Illumina PCR Primer Index 11 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGC	723	0.29674564834614575	Illumina PCR Primer Index 11 (95% over 23bp)
GAATGATCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTC	626	0.25693329995115805	No Hit
GAACTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCT	553	0.22697142950956933	Illumina PCR Primer Index 11 (95% over 21bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTAACACCTCGTA	494	0.20275567120746338	No Hit
ACTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTCTGC	428	0.17566685683561603	Illumina PCR Primer Index 11 (95% over 23bp)
GAATGAAACGGCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGC	396	0.162532886231084	No Hit
GAATCTATCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTTC	360	0.14775716930098545	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCG	358	0.1469362961382022	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	297	0.12189966467331301	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCTAACACCTCGTATGCCGTCTT	273	0.11204918671991397	No Hit
GGTGGGCAGGCAGGGATCTGGAAGGGTTGGGAAGATGTCTCAGCCTGATTT	256	0.10507176483625634	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGCGCA	45	3.8198777E-10	45.000004	22
CCGGCGC	45	3.8198777E-10	45.000004	21
TGAAACG	60	0.0	45.000004	4
ACGGGAT	45	3.8198777E-10	45.000004	5
CAGGGCG	30	2.1584365E-6	45.000004	5
GGGCGGC	30	2.1584365E-6	45.000004	7
GACGGGT	45	3.8198777E-10	45.000004	4
GTACGGC	30	2.1584365E-6	45.000004	10
TACCGGG	30	2.1584365E-6	45.000004	3
TAATCGG	30	2.1584365E-6	45.000004	2
TGATCGC	20	7.0223317E-4	45.0	37
TCACGAC	20	7.0223317E-4	45.0	25
AACCGTG	20	7.0223317E-4	45.0	1
TAATAGG	40	6.7775545E-9	45.0	3
TAGAGGA	40	6.7775545E-9	45.0	4
TCGCATA	20	7.0223317E-4	45.0	16
GCCCATA	20	7.0223317E-4	45.0	30
GCGACGT	40	6.7775545E-9	45.0	17
ATGATCG	20	7.0223317E-4	45.0	36
AGTCATG	25	3.8817285E-5	45.0	1

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content