##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936719.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	501149
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.148520699432705	31.0	31.0	33.0	28.0	34.0
2	31.19403610503064	31.0	31.0	34.0	28.0	34.0
3	30.918776651255413	31.0	31.0	34.0	27.0	34.0
4	34.89617059996129	35.0	35.0	37.0	32.0	37.0
5	35.179559372561855	35.0	35.0	37.0	33.0	37.0
6	35.181997769126546	36.0	35.0	37.0	33.0	37.0
7	31.098751070041047	35.0	35.0	37.0	0.0	37.0
8	33.13042428499308	36.0	35.0	37.0	17.0	37.0
9	36.38186846626452	39.0	35.0	39.0	32.0	39.0
10	36.21216843693193	37.0	35.0	39.0	32.0	39.0
11	36.430149516411284	38.0	35.0	39.0	32.0	39.0
12	36.42816008811751	38.0	35.0	39.0	32.0	39.0
13	36.31222450808043	38.0	35.0	39.0	32.0	39.0
14	37.28041360952531	39.0	36.0	40.0	32.0	41.0
15	37.46825993866096	39.0	36.0	41.0	32.0	41.0
16	37.458849563702614	39.0	36.0	41.0	32.0	41.0
17	37.39804329650463	39.0	36.0	40.0	32.0	41.0
18	37.27740252898838	39.0	36.0	40.0	32.0	41.0
19	37.057272388052255	39.0	35.0	40.0	32.0	41.0
20	36.98647108943648	39.0	35.0	40.0	32.0	41.0
21	36.959313497582556	39.0	35.0	40.0	32.0	41.0
22	37.06115546474202	39.0	35.0	40.0	32.0	41.0
23	37.14103789491748	39.0	35.0	40.0	32.0	41.0
24	37.05676355734522	39.0	35.0	40.0	32.0	41.0
25	36.77500104759263	38.0	35.0	40.0	31.0	41.0
26	36.763435624933905	38.0	35.0	40.0	31.0	41.0
27	36.79179645175387	38.0	35.0	40.0	31.0	41.0
28	36.812945850435696	39.0	35.0	40.0	32.0	41.0
29	36.77697650798465	39.0	35.0	40.0	31.0	41.0
30	36.56787502319669	38.0	35.0	40.0	31.0	41.0
31	36.47620967017793	38.0	35.0	40.0	31.0	41.0
32	36.25364113267711	38.0	35.0	40.0	30.0	41.0
33	36.089282828061116	38.0	35.0	40.0	30.0	41.0
34	35.9020411095303	38.0	35.0	40.0	29.0	41.0
35	35.798648705275276	38.0	35.0	40.0	29.0	41.0
36	35.708430027796126	38.0	35.0	40.0	28.0	41.0
37	35.610157857244054	38.0	35.0	40.0	28.0	41.0
38	35.457269195388996	38.0	35.0	40.0	27.0	41.0
39	35.41096759646333	38.0	35.0	40.0	27.0	41.0
40	35.297977248283445	38.0	34.0	40.0	26.0	41.0
41	35.115263125337975	38.0	34.0	40.0	25.0	41.0
42	35.14805177701642	38.0	34.0	40.0	25.0	41.0
43	35.099469419274506	38.0	34.0	40.0	25.0	41.0
44	34.88351967179422	38.0	34.0	40.0	24.0	41.0
45	34.78634098840864	38.0	34.0	40.0	24.0	41.0
46	34.68657824319713	38.0	34.0	40.0	24.0	41.0
47	34.630361429435155	37.0	34.0	40.0	23.0	41.0
48	34.54503949923077	37.0	34.0	40.0	23.0	41.0
49	34.48183673917338	37.0	34.0	40.0	24.0	41.0
50	34.29653456357291	37.0	33.0	40.0	23.0	41.0
51	32.589465408491286	35.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	16.0
9	37.0
10	53.0
11	46.0
12	34.0
13	16.0
14	22.0
15	35.0
16	68.0
17	143.0
18	253.0
19	464.0
20	780.0
21	1221.0
22	1843.0
23	2880.0
24	4218.0
25	5990.0
26	7650.0
27	8376.0
28	9033.0
29	9909.0
30	11729.0
31	14751.0
32	19181.0
33	26996.0
34	41220.0
35	45238.0
36	49106.0
37	71977.0
38	94048.0
39	73813.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.53325458097292	20.767675880825863	24.08505254924184	14.614016988959373
2	35.88872770373681	23.76019906255425	23.842609682948584	16.508463550760354
3	31.779171463975786	23.063998930457807	29.03507739215283	16.121752213413576
4	28.533629718905956	23.723483435066218	29.05842374224033	18.684463103787497
5	25.713709894662067	28.26983591706259	27.362121844002484	18.654332344272863
6	25.540906995723827	33.12687444253106	28.107409173718796	13.224809388026316
7	66.42276049637931	17.498189161307316	12.410680256769943	3.6683700855434207
8	76.59598243237042	6.514230298773419	12.472538107429127	4.417249161427041
9	71.01241347383713	7.39779985593107	14.652728030984797	6.937058639247011
10	42.37143045281942	22.456594745275357	21.261740520284388	13.910234281620834
11	31.242404953417047	24.524841913283275	26.241696581256274	17.991056552043403
12	27.62890876765194	22.7608954622278	31.197109043418227	18.413086726702037
13	24.128153503249532	24.93470005926381	33.70793915581993	17.22920728166673
14	20.115374868552067	27.036669732953673	32.908975175047736	19.93898022344652
15	17.92620557957813	24.003839177569944	39.28452416347234	18.785431079379585
16	21.679380782960756	24.81477564556649	34.29079974219244	19.215043829280315
17	22.748523892095964	23.416588679215163	31.401040409139796	22.433847019549077
18	23.335973931904483	23.47226074480843	33.53812937868778	19.65363594459931
19	24.141522780650064	25.036665742124598	30.39934231136848	20.42246916585686
20	25.027686376706328	25.687569964222217	30.820973403119634	18.46377025595182
21	23.597772319210456	25.85099441483471	32.70743830677104	17.843794959183796
22	22.521246176286894	22.92531762010899	32.02281157899148	22.530624624612642
23	21.11108672271121	25.52175101616485	32.68868141011955	20.67848085100439
24	21.07676559266805	23.96991713043426	32.72639474487627	22.226922532021415
25	20.64415972096123	26.186822681477963	31.84282518771862	21.326192409842182
26	20.09761567916927	25.251571887801834	31.974921630094045	22.675890802934855
27	20.790423606552146	26.182632310949437	32.5859175614438	20.441026521054617
28	18.68645851832489	25.26913153573089	33.797732810002614	22.246677135941606
29	20.30992778594789	24.991569373579516	33.82886127678594	20.86964156368665
30	21.516155873802003	24.44283037579642	34.0732995576166	19.96771419278498
31	24.266435730690873	23.865556950128607	30.866468854572194	21.00153846460833
32	23.261944052567202	25.45869591678323	31.084567663509254	20.194792367140312
33	23.36849918886399	24.132942498139276	30.49272771171847	22.005830601278262
34	21.812275391151132	23.831036278631704	32.703846560603736	21.652841769613428
35	19.74662226204183	25.66681765303333	32.23292872977897	22.353631355145875
36	23.72328389361248	25.288886139651083	30.456211625684176	20.531618341052262
37	22.063498081408923	26.201389207600933	31.0771846297209	20.657928081269244
38	21.205669371783642	26.928917347934444	29.626917343943614	22.238495936338296
39	21.860165340048567	24.2005870509569	31.367916527819073	22.571331081175458
40	23.128849902923083	23.271721583800424	31.21287281826363	22.38655569501286
41	20.1977854889464	24.06050895043191	29.670616922312526	26.071088638309163
42	21.809082727891305	23.473857076438346	30.80281513082935	23.914245064840994
43	22.099415543082	23.654043009164937	30.06411266908644	24.182428778666623
44	21.759995530271436	24.286190334611064	30.644578758014084	23.309235377103416
45	21.310827717904257	24.58669976394246	29.93401164124841	24.168460876904874
46	22.46736998377728	24.948069336664343	30.296378921238993	22.288181758319382
47	20.16386344181072	24.380573442229757	32.70205068752008	22.753512428439446
48	21.054416949849248	24.762495784686788	31.187331512185	22.995755753278964
49	21.92282135652271	22.862062979273627	32.92194536954079	22.293170294662865
50	20.350434701056972	24.57233277927323	31.433166583191824	23.644065936477972
51	20.205767147095973	24.119174137831266	29.52455257817535	26.150506136897413
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	176.0
1	516.5
2	857.0
3	4806.0
4	8755.0
5	5951.5
6	3148.0
7	2907.5
8	2667.0
9	2663.5
10	2660.0
11	2541.5
12	2423.0
13	2352.5
14	2282.0
15	2162.0
16	2042.0
17	1978.0
18	1914.0
19	1996.5
20	2079.0
21	2324.0
22	2569.0
23	2680.0
24	2791.0
25	3070.5
26	3878.5
27	4407.0
28	5242.5
29	6078.0
30	7403.0
31	8728.0
32	9926.0
33	11124.0
34	12637.0
35	14150.0
36	15493.5
37	16837.0
38	17980.5
39	19124.0
40	21527.0
41	23930.0
42	26727.0
43	29524.0
44	32062.5
45	34601.0
46	41394.0
47	48187.0
48	46399.0
49	44611.0
50	43109.5
51	41608.0
52	36989.5
53	32371.0
54	29231.0
55	26091.0
56	24263.0
57	22435.0
58	20688.0
59	18941.0
60	17634.0
61	16327.0
62	15029.5
63	13732.0
64	11754.5
65	9777.0
66	8392.5
67	7008.0
68	5920.0
69	4832.0
70	4297.5
71	3763.0
72	3002.0
73	2241.0
74	1876.0
75	1142.0
76	773.0
77	580.5
78	388.0
79	274.5
80	161.0
81	108.5
82	56.0
83	45.5
84	35.0
85	33.0
86	31.0
87	31.5
88	32.0
89	23.0
90	14.0
91	7.5
92	1.0
93	2.0
94	3.0
95	1.5
96	0.0
97	2.0
98	4.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	501149.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.08110771583605
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.74725216640084	24.727219003794655
2	9.633049722902538	6.373439110306868
3	3.693199094348037	3.6652535116846665
4	2.0873723468410152	2.7621035779562044
5	1.399450746872449	2.3147690450147347
6	1.0207279356999444	2.02600864736717
7	0.7290223716467539	1.6881807322590365
8	0.5386064920690837	1.4254159504468762
9	0.4309644547286064	1.2831103393716217
>10	5.366549816244396	43.161456056933844
>50	0.3231176966943763	6.404696510421047
>100	0.02647519302523754	1.5217523875611918
>500	0.0012034178647835243	0.2838481235660248
>1k	0.003008544661958811	2.362747003316069
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4908	0.9793494549525191	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCG	2142	0.4274177939095958	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGC	1954	0.389904000606606	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTC	1563	0.311883292194537	No Hit
GCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCTGC	1303	0.2600025142223171	TruSeq Adapter, Index 21 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCTGC	868	0.17320198184571856	TruSeq Adapter, Index 21 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTTGTTGCCTCGTATGCCGTCTTCTGCT	558	0.11134413118653334	TruSeq Adapter, Index 14 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.13608727145020744	0.0
2	0.0	0.0	0.0	0.8087415120054116	0.0
3	0.0	0.0	0.0	1.0517830026598876	0.0
4	0.0	0.0	0.0	1.4414874618127542	0.0
5	0.0	0.0	0.0	2.645321052221994	0.0
6	0.0	0.0	0.0	3.1326012822533817	0.0
7	0.0	0.0	0.0	3.729828853295128	0.0
8	0.0	0.0	0.0	4.655900740099252	0.0
9	0.0	0.0	0.0	4.97317165154475	0.0
10	0.0	0.0	0.0	6.147473106800573	0.0
11	0.0	0.0	0.0	7.24235706346815	0.0
12	0.0	0.0	0.0	8.499468222025785	0.0
13	0.0	0.0	0.0	8.8752047794169	0.0
14	0.0	0.0	0.0	9.037431981306957	0.0
15	0.0	0.0	0.0	9.39081989587927	0.0
16	0.0	0.0	0.0	10.027756216215138	0.0
17	0.0	0.0	0.0	10.70659624183626	0.0
18	0.0	0.0	0.0	11.533695567585688	0.0
19	1.9954145373930708E-4	0.0	0.0	11.997828988983317	0.0
20	1.9954145373930708E-4	0.0	0.0	12.488501423728273	0.0
21	1.9954145373930708E-4	0.0	0.0	13.064976683581131	0.0
22	1.9954145373930708E-4	0.0	0.0	13.639456528896595	0.0
23	1.9954145373930708E-4	0.0	0.0	14.201365262626485	0.0
24	1.9954145373930708E-4	0.0	0.0	14.650732616447403	0.0
25	1.9954145373930708E-4	0.0	0.0	15.070168752207428	0.0
26	1.9954145373930708E-4	0.0	0.0	15.440517690347582	0.0
27	1.9954145373930708E-4	0.0	0.0	15.837605183288803	0.0
28	1.9954145373930708E-4	0.0	0.0	16.256642236141346	0.0
29	1.9954145373930708E-4	0.0	0.0	16.698826097627652	0.0
30	1.9954145373930708E-4	0.0	0.0	17.23220040347282	0.0
31	1.9954145373930708E-4	0.0	0.0	17.688551708174614	0.0
32	1.9954145373930708E-4	0.0	0.0	18.11557041917673	0.0
33	1.9954145373930708E-4	0.0	0.0	18.52183681898996	0.0
34	1.9954145373930708E-4	0.0	0.0	18.95444269069678	0.0
35	1.9954145373930708E-4	0.0	0.0	19.440126589098252	0.0
36	1.9954145373930708E-4	0.0	0.0	19.8404067452993	0.0
37	1.9954145373930708E-4	0.0	0.0	20.27600573881221	0.0
38	1.9954145373930708E-4	0.0	0.0	20.722779053734516	0.0
39	1.9954145373930708E-4	0.0	0.0	21.364903451867608	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAAGTCG	20	7.0294726E-4	45.000004	27
CACGACC	40	6.8012014E-9	45.000004	27
AATGCGA	20	7.0294726E-4	45.000004	37
GTATGCG	30	2.1630422E-6	45.000004	1
GCAATCG	30	2.1630422E-6	45.000004	21
CGGTCAG	20	7.0294726E-4	45.000004	28
CGCAATC	30	2.1630422E-6	45.000004	20
TAAGCGA	20	7.0294726E-4	45.000004	1
CATGCGG	25	3.8876457E-5	45.0	2
GGGTTAC	25	3.8876457E-5	45.0	7
GCGTAAG	25	3.8876457E-5	45.0	1
CGTTTTT	2395	0.0	43.121086	1
TGCACGG	70	0.0	41.785713	2
ACAACGA	110	0.0	40.909092	13
TATGCGG	50	1.0786607E-9	40.5	2
ACACGAC	45	1.9244908E-8	40.0	26
GACGGGT	90	0.0	40.0	4
TACGGGA	85	0.0	39.705883	4
AAGGTCG	40	3.453024E-7	39.375004	1
CGTTTTA	225	0.0	39.0	1
>>END_MODULE