##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936718.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	653007
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.12506144650823	31.0	31.0	33.0	28.0	34.0
2	31.156159122337126	31.0	31.0	34.0	28.0	34.0
3	30.855323143549764	31.0	30.0	34.0	27.0	34.0
4	34.82721012179042	35.0	35.0	37.0	32.0	37.0
5	35.11850715229699	35.0	35.0	37.0	33.0	37.0
6	35.10065895158857	36.0	35.0	37.0	32.0	37.0
7	31.253433730419427	35.0	35.0	37.0	0.0	37.0
8	33.18037632062137	36.0	35.0	37.0	17.0	37.0
9	36.446347435785526	39.0	35.0	39.0	32.0	39.0
10	36.1350077411115	37.0	35.0	39.0	32.0	39.0
11	36.33349565931146	38.0	35.0	39.0	32.0	39.0
12	36.545986490190764	38.0	35.0	39.0	32.0	39.0
13	36.54676442978406	39.0	35.0	39.0	32.0	39.0
14	37.69228048091368	39.0	37.0	41.0	33.0	41.0
15	37.794698984850086	39.0	37.0	41.0	33.0	41.0
16	37.764910636486285	39.0	37.0	41.0	33.0	41.0
17	37.612108292866694	39.0	36.0	41.0	32.0	41.0
18	37.50777097335863	39.0	36.0	40.0	32.0	41.0
19	37.285306283087316	39.0	36.0	40.0	32.0	41.0
20	37.20250778322438	39.0	35.0	40.0	32.0	41.0
21	37.30330455875664	39.0	35.0	40.0	32.0	41.0
22	37.38011843670895	39.0	35.0	40.0	32.0	41.0
23	37.4269663265478	39.0	35.0	40.0	33.0	41.0
24	37.35995479374647	39.0	35.0	41.0	32.0	41.0
25	37.08193786590343	39.0	35.0	40.0	32.0	41.0
26	37.057382233268555	39.0	35.0	40.0	32.0	41.0
27	37.12088691239144	39.0	35.0	40.0	32.0	41.0
28	37.10703560605017	39.0	35.0	40.0	32.0	41.0
29	37.08970041668772	39.0	35.0	40.0	32.0	41.0
30	36.92871133081269	39.0	35.0	40.0	31.0	41.0
31	36.87584053463439	39.0	35.0	40.0	31.0	41.0
32	36.69241064797161	39.0	35.0	40.0	31.0	41.0
33	36.53276151710472	39.0	35.0	40.0	30.0	41.0
34	36.40366182904624	39.0	35.0	40.0	30.0	41.0
35	36.333074530594615	39.0	35.0	40.0	30.0	41.0
36	36.185884684237685	39.0	35.0	40.0	30.0	41.0
37	36.09658395698668	39.0	35.0	40.0	29.0	41.0
38	36.04463504985398	39.0	35.0	40.0	29.0	41.0
39	35.97466336501753	39.0	35.0	40.0	29.0	41.0
40	35.90437621648773	39.0	35.0	40.0	29.0	41.0
41	35.777138683046275	39.0	35.0	40.0	28.0	41.0
42	35.76266104344976	39.0	35.0	40.0	28.0	41.0
43	35.74723854415037	39.0	35.0	40.0	28.0	41.0
44	35.55443662931638	38.0	35.0	40.0	27.0	41.0
45	35.50768521623811	38.0	35.0	40.0	26.0	41.0
46	35.42894639720554	38.0	35.0	40.0	26.0	41.0
47	35.369633097348114	38.0	35.0	40.0	26.0	41.0
48	35.29754198653307	38.0	34.0	40.0	26.0	41.0
49	35.21956119919082	38.0	34.0	40.0	26.0	41.0
50	35.06826573068895	38.0	34.0	40.0	25.0	41.0
51	33.400980387652815	37.0	32.0	39.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
6	1.0
7	3.0
8	19.0
9	32.0
10	30.0
11	22.0
12	13.0
13	9.0
14	22.0
15	22.0
16	68.0
17	128.0
18	294.0
19	549.0
20	989.0
21	1508.0
22	2233.0
23	3256.0
24	4784.0
25	6786.0
26	8640.0
27	9553.0
28	10224.0
29	11406.0
30	13471.0
31	17151.0
32	22578.0
33	30741.0
34	44901.0
35	53855.0
36	64186.0
37	97401.0
38	134989.0
39	113140.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.95271107354132	21.026727125436633	25.924836946617724	13.095724854404317
2	32.39475227677498	27.008898832631196	25.89757843330929	14.698770457284532
3	28.923579685975803	26.625441993730544	29.94041411500949	14.51056420528417
4	25.483647189080667	24.902489559836265	32.54344899824964	17.07041425283343
5	26.99281937253353	28.056820217853712	27.561725984560653	17.388634425052103
6	24.863439442456205	34.939135415087435	26.97980266674017	13.217622475716187
7	68.38043083764799	15.669204158607794	12.776432718179132	3.1739322855650856
8	80.05947868858986	6.070991582019794	10.025619939755623	3.8439097896347203
9	74.6872545011003	6.509884273828612	11.440153015205045	7.36270820986605
10	35.83927890512659	35.02427998474749	17.550807265465764	11.585633844660165
11	23.46820171912399	25.952248597641375	33.46641000785596	17.113139675378672
12	22.2723493010029	23.288571179175722	35.529787582675226	18.909291937146154
13	21.097629887581604	25.553477987219125	36.39593449993645	16.952957625262822
14	18.887240106155065	29.11423614141962	32.15172272272732	19.846801029697996
15	17.768875371933227	28.181933731185115	36.988271182391614	17.060919714490044
16	21.02994301745617	27.42390204086633	31.465972033990447	20.080182907687057
17	20.95077081868954	27.03814813623744	31.47179126716865	20.539289777904372
18	21.03178067003876	25.959292932541306	32.85263404527058	20.156292352149364
19	20.847709136349227	28.509801579462398	30.494160093230242	20.148329190958137
20	21.27327884693426	27.256063104989686	33.55477046953555	17.915887578540506
21	21.827637376015876	28.17626763572213	31.93763619685547	18.058458791406522
22	20.688139637094245	24.190246046367037	32.839157926331566	22.28245639020715
23	20.080489183117486	27.377960726301552	32.39567110306628	20.14587898751468
24	20.881858846842377	26.300024348896716	31.809613066934965	21.00850373732594
25	19.181877070230488	30.223718888158935	29.872574107168838	20.721829934441743
26	19.7025453019646	28.868450108498074	30.231682049350162	21.197322540187166
27	19.687384668158227	29.285290969315795	30.405187080689792	20.622137281836185
28	17.36597004320015	28.71515925556694	33.633942668302176	20.284928032930736
29	19.6553788856781	26.77965167295297	32.77560577451696	20.789363666851962
30	19.210513822975862	28.233234865782446	33.08724102498136	19.46901028626033
31	21.76071619446652	28.729860476227664	28.86768441992199	20.641738909383818
32	23.00434757973498	28.6041344120354	28.527259278997008	19.864258729232613
33	20.53438937101746	27.732015123880753	28.83552549972665	22.898070005375136
34	21.634224441698173	29.65894699444263	28.917760452797598	19.789068111061596
35	22.065766523176627	26.625441993730544	30.06246487403657	21.246326609056258
36	22.455808283831566	29.578090280808627	28.906581399586834	19.05952003577297
37	21.071290200564466	28.10368035870978	31.916350054440457	18.908679386285293
38	21.005747258452054	28.899383926971687	28.118687854800946	21.976180959775316
39	22.454276906679407	28.49402839479516	29.199074435649237	19.852620262876204
40	22.059181601422342	27.107060108084603	29.62295963136689	21.210798659126166
41	20.99456820524129	29.200758950516608	28.957729396468952	20.846943447773146
42	22.5783184560043	26.617938245684964	30.587880374942383	20.215862923368356
43	23.42317923085051	26.41058977928261	28.22083071084996	21.945400279016916
44	20.77864402678685	27.168774607316614	29.539346438859	22.513234927037534
45	20.92106210193765	27.36923187653425	28.197706915852354	23.511999105675745
46	22.91920301007493	27.75544519430879	28.476417557545325	20.848934238070953
47	19.830415294169896	26.48792432546665	31.991081259465826	21.69057912089763
48	21.175883260056935	26.62467630515446	29.6480742166623	22.551366218126297
49	20.339291921832384	25.756232322165	32.30577926423454	21.59869649176808
50	21.093188893840342	25.22438503722012	30.54745201812538	23.134974050814154
51	20.319077743423883	25.405087541174904	29.573343011636933	24.702491703764277
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	190.0
1	396.5
2	603.0
3	4989.0
4	9375.0
5	6469.5
6	3564.0
7	3323.5
8	3083.0
9	3070.0
10	3057.0
11	3022.0
12	2987.0
13	2865.5
14	2744.0
15	2726.0
16	2708.0
17	2655.0
18	2602.0
19	2527.5
20	2453.0
21	2638.5
22	2824.0
23	3363.0
24	3902.0
25	4625.0
26	6745.0
27	8142.0
28	9635.5
29	11129.0
30	12556.5
31	13984.0
32	16302.5
33	18621.0
34	20686.5
35	22752.0
36	25359.0
37	27966.0
38	29934.5
39	31903.0
40	34131.5
41	36360.0
42	40166.5
43	43973.0
44	47788.5
45	51604.0
46	68722.5
47	85841.0
48	72567.0
49	59293.0
50	55993.5
51	52694.0
52	45733.0
53	38772.0
54	34281.5
55	29791.0
56	26300.5
57	22810.0
58	19962.0
59	17114.0
60	15274.0
61	13434.0
62	11258.0
63	9082.0
64	7225.5
65	5369.0
66	4135.5
67	2902.0
68	2445.0
69	1988.0
70	1505.5
71	1023.0
72	777.0
73	531.0
74	388.5
75	181.0
76	116.0
77	85.5
78	55.0
79	46.0
80	37.0
81	26.5
82	16.0
83	13.5
84	11.0
85	7.0
86	3.0
87	2.5
88	2.0
89	1.5
90	1.0
91	0.5
92	0.0
93	0.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	653007.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.09941243495438
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.59320766958756	26.181777608410563
2	8.991186600579509	6.3117073354675135
3	3.669293285530939	3.8637011512097774
4	2.2394391166056935	3.1441198870685256
5	1.5074117218994136	2.645463286811614
6	1.0985053094932584	2.3134134551934653
7	0.8560677362064246	2.103323218175692
8	0.6715753836474746	1.8857521097444343
9	0.5395908940609951	1.7045391003113382
>10	5.691584711331749	40.28375269391165
>50	0.11518793985857002	2.4918789733179985
>100	0.020864230605214584	1.3630347601737278
>500	0.003042700296593794	0.7179809247120621
>1k	0.0013040144128259115	1.246320903946629
>5k	0.0017386858837678822	3.7432345915449314
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTC	6963	1.0662979110484267	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCG	6009	0.9202045307324423	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGC	5968	0.9139258844085898	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5595	0.8568055166330529	No Hit
GAACTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTCT	4772	0.7307731770103536	No Hit
GCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTCTGC	2111	0.32327371682079975	Illumina Single End Adapter 2 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCT	1286	0.19693510176766862	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTC	940	0.14394945230296152	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCATAGCAGGTCGTA	731	0.11194366982283499	No Hit
GAATCTATCTCTTATACACATCTGACGCATAGCAGGTCGTATGCCGTCTTC	728	0.11148425667718723	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09479224571865233	0.0
2	0.0	0.0	0.0	0.6730402583739531	0.0
3	0.0	0.0	0.0	0.8829920659349747	0.0
4	0.0	0.0	0.0	1.9272381459923094	0.0
5	0.0	0.0	0.0	3.643605658132302	0.0
6	0.0	0.0	0.0	4.2039365581073405	0.0
7	0.0	0.0	0.0	4.811740149799313	0.0
8	0.0	0.0	0.0	5.656600924645525	0.0
9	0.0	0.0	0.0	5.92229486054514	0.0
10	0.0	0.0	0.0	7.533456762331797	0.0
11	0.0	0.0	0.0	8.30557712245045	0.0
12	0.0	0.0	0.0	9.994992396712439	0.0
13	0.0	0.0	0.0	10.372783140150105	0.0
14	0.0	0.0	0.0	10.532199501689874	0.0
15	0.0	0.0	0.0	10.843834752154265	0.0
16	0.0	0.0	0.0	11.276601935354446	0.0
17	1.5313771521591651E-4	0.0	0.0	11.848724439401108	0.0
18	1.5313771521591651E-4	0.0	0.0	12.480417514666765	0.0
19	1.5313771521591651E-4	0.0	0.0	12.921300996773388	0.0
20	1.5313771521591651E-4	0.0	0.0	13.316243164315237	0.0
21	1.5313771521591651E-4	0.0	0.0	13.75191996410452	0.0
22	1.5313771521591651E-4	0.0	0.0	14.258346388323556	0.0
23	1.5313771521591651E-4	0.0	0.0	14.749612178736216	0.0
24	1.5313771521591651E-4	0.0	0.0	15.1439417954172	0.0
25	1.5313771521591651E-4	0.0	0.0	15.485898313494342	0.0
26	1.5313771521591651E-4	0.0	0.0	15.817288329221586	0.0
27	1.5313771521591651E-4	0.0	0.0	16.161695050742182	0.0
28	1.5313771521591651E-4	0.0	0.0	16.513452382593144	0.0
29	1.5313771521591651E-4	0.0	0.0	16.878838971098318	0.0
30	1.5313771521591651E-4	0.0	0.0	17.32661365038966	0.0
31	1.5313771521591651E-4	0.0	0.0	17.733500559718347	0.0
32	1.5313771521591651E-4	0.0	0.0	18.107462860275618	0.0
33	1.5313771521591651E-4	0.0	0.0	18.472083760204715	0.0
34	1.5313771521591651E-4	0.0	0.0	18.85523432367494	0.0
35	1.5313771521591651E-4	0.0	0.0	19.26549026273838	0.0
36	1.5313771521591651E-4	0.0	0.0	19.65813536455199	0.0
37	1.5313771521591651E-4	0.0	0.0	20.028422359944074	0.0
38	1.5313771521591651E-4	0.0	0.0	20.418770396029444	0.0
39	1.5313771521591651E-4	0.0	0.0	20.810649809266977	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTTCGCG	25	3.888948E-5	45.000004	1
CTACGCG	25	3.888948E-5	45.000004	1
CGACGGT	35	1.2108467E-7	45.0	28
CGCTAAG	20	7.031046E-4	45.0	2
TGTCGCG	20	7.031046E-4	45.0	1
TATTGCG	60	0.0	44.999996	1
CGTTTTT	3020	0.0	43.36093	1
GCGATTT	65	0.0	41.53846	9
GTTTGCG	55	6.002665E-11	40.909092	1
TCGCTCA	45	1.9261279E-8	40.0	11
GGACGAT	45	1.9261279E-8	40.0	8
GGATCGC	85	0.0	39.705883	8
CTATGCG	40	3.4551704E-7	39.375	1
CTCACGA	40	3.4551704E-7	39.375	24
CGGTCTA	40	3.4551704E-7	39.375	31
GCGGGCT	75	0.0	39.0	5
TACGGCT	745	0.0	38.959732	7
AACGGCT	110	0.0	38.86364	7
ACCGGCG	35	6.244145E-6	38.571426	20
CCAGCGG	35	6.244145E-6	38.571426	2
>>END_MODULE