SRR2936716.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2936716.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	195439
Sequences flagged as poor quality	0
Sequence length	51
%GC	42

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4799	2.4554976232993417	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCG	1912	0.9783103679408921	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGC	1730	0.8851866822896147	No Hit
GCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGC	1605	0.8212281069796714	Illumina PCR Primer Index 3 (95% over 22bp)
GAATCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTC	1491	0.7628978862970032	No Hit
CCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGC	1488	0.7613628804895645	RNA PCR Primer, Index 7 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGCT	896	0.4584550678216732	TruSeq Adapter, Index 7 (95% over 24bp)
TCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGC	523	0.2676026790968026	Illumina PCR Primer Index 3 (95% over 22bp)
GAATGACTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCT	434	0.222064173476123	No Hit
ACTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTGC	376	0.19238739453230932	TruSeq Adapter, Index 7 (95% over 23bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCC	354	0.1811306852777593	No Hit
ACACAAGGGATTGAGGTGTTATTAGCACTTTTGTTCATAATTCATAATTTT	352	0.1801073480728002	No Hit
CGCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCTG	347	0.1775490050604025	TruSeq Adapter, Index 7 (95% over 22bp)
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	335	0.17140898183064793	No Hit
GAACTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTTCT	320	0.16373395279345473	TruSeq Adapter, Index 7 (95% over 21bp)
GAATGCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTCTT	306	0.1565705923587411	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCGTC	287	0.1468488889116297	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTAGTCACCTCGTA	266	0.13610384825955926	No Hit
TGTAGGAGGGGATATCAAGAAGGAAAAAGATGGGTGGCCGAGTGACTCTCA	251	0.12842881922236607	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGC	233	0.11921878437773423	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCTAGTCACCTCGTATGCCG	216	0.11052041813558194	No Hit
AATGTGGGGCAGCATGGGTTTCTGATATTCCTTTAATAAGGTGGATAGTGC	196	0.10028704608599102	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACGGGGT	35	1.2050259E-7	45.000004	5
AGGGCTC	35	1.2050259E-7	45.000004	6
GGCTAGA	35	1.2050259E-7	45.000004	14
TCAGTTA	35	1.2050259E-7	45.000004	44
AGACTTA	20	7.018905E-4	45.0	22
CGGGTCT	80	0.0	45.0	6
CGGAAGG	20	7.018905E-4	45.0	2
CAAGTTG	25	3.8788894E-5	45.0	15
GTATTAG	25	3.8788894E-5	45.0	22
TTGTGCG	20	7.018905E-4	45.0	1
AGTCAAT	25	3.8788894E-5	45.0	8
TAGACTA	20	7.018905E-4	45.0	20
GGCGTAG	20	7.018905E-4	45.0	1
AAGACTT	20	7.018905E-4	45.0	21
GCGCCAT	20	7.018905E-4	45.0	22
CCAGCGC	20	7.018905E-4	45.0	19
AAGCGGC	25	3.8788894E-5	45.0	22
TCGATCA	25	3.8788894E-5	45.0	17
ACCCTAC	20	7.018905E-4	45.0	15
TAGTTGA	20	7.018905E-4	45.0	42

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content