##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936715.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	542360
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.091160852570248	31.0	31.0	33.0	28.0	34.0
2	31.138212626299875	31.0	31.0	34.0	28.0	34.0
3	30.89483000221255	31.0	31.0	34.0	27.0	34.0
4	34.842294785751164	35.0	35.0	37.0	32.0	37.0
5	35.10456154583672	35.0	35.0	37.0	33.0	37.0
6	35.070969466774834	35.0	35.0	37.0	32.0	37.0
7	31.056350025813114	35.0	35.0	37.0	0.0	37.0
8	33.0300298694594	35.0	35.0	37.0	17.0	37.0
9	36.24934360941072	39.0	35.0	39.0	32.0	39.0
10	35.93181281805443	37.0	35.0	39.0	32.0	39.0
11	36.238325097721074	37.0	35.0	39.0	32.0	39.0
12	36.416962902868946	38.0	35.0	39.0	32.0	39.0
13	36.348180175529166	38.0	35.0	39.0	32.0	39.0
14	37.46959215281363	39.0	36.0	40.0	32.0	41.0
15	37.56477985102146	39.0	36.0	41.0	32.0	41.0
16	37.579611328269046	39.0	36.0	40.0	33.0	41.0
17	37.41495501143152	39.0	36.0	40.0	32.0	41.0
18	37.31584372003835	39.0	36.0	40.0	32.0	41.0
19	37.107340143078396	39.0	36.0	40.0	32.0	41.0
20	37.03791208791209	39.0	35.0	40.0	32.0	41.0
21	37.16196622169777	39.0	35.0	40.0	32.0	41.0
22	37.25045357327237	39.0	35.0	40.0	32.0	41.0
23	37.301403127074266	39.0	35.0	40.0	32.0	41.0
24	37.20286525554982	39.0	35.0	40.0	32.0	41.0
25	36.94479128254296	39.0	35.0	40.0	31.0	41.0
26	36.9054207537429	39.0	35.0	40.0	31.0	41.0
27	36.99104284976768	39.0	35.0	40.0	32.0	41.0
28	36.99479865771812	39.0	35.0	40.0	31.0	41.0
29	36.97710376871451	39.0	35.0	40.0	31.0	41.0
30	36.783724832214766	39.0	35.0	40.0	31.0	41.0
31	36.769951692602696	39.0	35.0	40.0	31.0	41.0
32	36.58770005162623	39.0	35.0	40.0	30.0	41.0
33	36.44205509255845	39.0	35.0	40.0	30.0	41.0
34	36.295217198908475	39.0	35.0	40.0	30.0	41.0
35	36.2551607788185	39.0	35.0	40.0	30.0	41.0
36	36.17973301865919	39.0	35.0	40.0	30.0	41.0
37	36.048875285788036	39.0	35.0	40.0	29.0	41.0
38	36.00328748432775	39.0	35.0	40.0	29.0	41.0
39	35.92882034073309	38.0	35.0	40.0	29.0	41.0
40	35.87765875064533	38.0	35.0	40.0	29.0	41.0
41	35.72421454384541	38.0	35.0	40.0	28.0	41.0
42	35.70839663692013	38.0	35.0	40.0	28.0	41.0
43	35.70011984659636	38.0	35.0	40.0	28.0	41.0
44	35.50687550704329	38.0	35.0	40.0	27.0	41.0
45	35.488870860682944	38.0	35.0	40.0	27.0	41.0
46	35.391356294712	38.0	34.0	40.0	27.0	41.0
47	35.31442031123239	38.0	34.0	40.0	26.0	41.0
48	35.277365587432705	38.0	34.0	40.0	26.0	41.0
49	35.171483885242274	38.0	34.0	40.0	26.0	41.0
50	35.013039309683606	38.0	34.0	40.0	26.0	41.0
51	33.26541964746663	36.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	11.0
9	33.0
10	31.0
11	31.0
12	12.0
13	15.0
14	15.0
15	29.0
16	67.0
17	108.0
18	272.0
19	443.0
20	837.0
21	1242.0
22	1817.0
23	2667.0
24	3728.0
25	5441.0
26	6728.0
27	7720.0
28	8439.0
29	9760.0
30	12025.0
31	15286.0
32	20554.0
33	27725.0
34	40614.0
35	47072.0
36	54920.0
37	80103.0
38	108182.0
39	86429.0
40	4.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.427354524669965	20.319160705066746	24.48871598200457	12.76476878825872
2	31.65757061730216	27.47086805811638	25.17423851316469	15.697322811416772
3	28.9173242864518	26.436868500626893	28.96507854561546	15.680728667305848
4	26.061472084962016	23.93189025739361	32.85953978906999	17.147097868574377
5	26.60631314993731	28.206910539125303	27.21384320377609	17.972933107161296
6	23.62102662438233	36.36108857585368	27.119625341101848	12.898259458662142
7	66.09668854635298	16.89265432554023	13.453241389483	3.5574157386237926
8	77.67552916881776	7.675529168817759	10.545025444354303	4.103916218010178
9	72.9297883324729	5.803894092484697	11.732428645180324	9.533888929862085
10	38.49896747547754	24.77819160705067	20.992329817833173	15.730511099638617
11	27.27395088133343	25.554797551441848	28.459879047127366	18.711372520097353
12	24.39873884504757	22.66041005973892	32.435835976104435	20.50501511910908
13	21.316468766133195	24.504388229220446	35.6582343830666	18.520908621579764
14	19.100966147946014	28.156021830518473	31.406261523711187	21.336750497824323
15	18.198613467069844	26.214691348919537	37.237259384910395	18.349435799100227
16	20.250940334832954	26.435946603731843	30.794306364776165	22.51880669665905
17	21.081753816653144	25.38940924846965	31.982815841876246	21.54602109300096
18	20.971126189246995	25.55682572461096	32.863596135408216	20.60845195073383
19	21.75307913562947	27.72955232686776	29.498856847850135	21.01851168965263
20	21.696659045652332	26.769304520982374	33.18220370233793	18.35183273102736
21	21.37270447673132	29.20182166826462	30.808319197580943	18.617154657423114
22	20.88778670993436	23.83472232465521	31.247879637141384	24.029611328269045
23	19.329965336676747	28.441441109226346	31.371413821078253	20.85717973301866
24	21.3057747621506	24.805110996386166	31.20952872630725	22.679585515155985
25	18.970056788848737	30.273250239693194	29.94837377387713	20.808319197580943
26	18.918246183346852	26.519470462423484	31.946861862969243	22.615421491260417
27	20.157828748432774	27.473818128180543	30.20576738697544	22.162585736411238
28	16.786451803230324	26.103879342134377	35.761855594070354	21.347813260564937
29	20.01364407404676	24.510841507485804	33.18386311674902	22.291651301718414
30	18.887639206431153	27.016926026993143	31.662180101777416	22.433254664798287
31	21.006895788774983	26.368463751014087	30.764990043513535	21.859650416697395
32	21.4801976546943	28.68039678442363	29.27465152297367	20.5647540379084
33	20.486761560587063	26.069400398259457	28.368795633896305	25.07504240725717
34	20.14787226196622	27.056383214101338	31.593775352164617	21.20196917176783
35	21.067187845711334	25.55811638026403	28.504683236226857	24.870012537797773
36	22.098421712515673	25.927981414558598	30.830629102441183	21.14296777048455
37	19.591968434250315	28.23807065417804	30.93572534847703	21.23423556309462
38	20.346817611918283	27.072239840696216	29.22247215871377	23.35847038867173
39	21.118260933697176	28.37137694520245	29.178774245888338	21.331587875212037
40	21.6708459325909	25.67280035400841	29.88420974998156	22.77214396341913
41	21.005051994984882	27.472711851906485	28.756729847333872	22.765506305774764
42	21.993141087100817	24.417176782948598	31.798436462865993	21.791245667084596
43	22.88332472896231	25.119662216977652	28.5773287115569	23.419684342503132
44	20.391806180396784	25.39715318238808	30.228630429972714	23.98241020724242
45	19.98432775278413	25.591489047864886	29.139132679401136	25.28505051994985
46	23.141271480197656	27.396010030238216	28.141640238955674	21.32107825060845
47	18.589128991813556	25.213695700272883	33.97595692897706	22.2212183789365
48	21.301534036433363	26.036396489416624	29.599343609410724	23.062725864739285
49	20.11081200678516	23.551699977874474	33.27089018364186	23.066597831698502
50	20.044619809720484	24.518954200162256	31.493657349362046	23.94276864075522
51	20.02065049044915	24.10446935614721	28.920827494652997	26.954052658750644
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	144.0
1	410.0
2	676.0
3	3959.0
4	7242.0
5	4933.5
6	2625.0
7	2469.5
8	2314.0
9	2222.0
10	2130.0
11	2042.5
12	1955.0
13	1900.0
14	1845.0
15	1801.0
16	1757.0
17	1713.5
18	1670.0
19	1753.5
20	1837.0
21	2028.0
22	2219.0
23	2404.0
24	2589.0
25	3219.5
26	4595.0
27	5340.0
28	6421.0
29	7502.0
30	8910.5
31	10319.0
32	12225.5
33	14132.0
34	16530.5
35	18929.0
36	19915.0
37	20901.0
38	22360.5
39	23820.0
40	26147.0
41	28474.0
42	31343.5
43	34213.0
44	37108.0
45	40003.0
46	56627.0
47	73251.0
48	61918.5
49	50586.0
50	48438.5
51	46291.0
52	39715.5
53	33140.0
54	29562.0
55	25984.0
56	23444.0
57	20904.0
58	18486.0
59	16068.0
60	14580.5
61	13093.0
62	11329.0
63	9565.0
64	7943.0
65	6321.0
66	5031.0
67	3741.0
68	3177.5
69	2614.0
70	2171.0
71	1728.0
72	1406.5
73	1085.0
74	931.5
75	525.0
76	272.0
77	241.0
78	210.0
79	170.5
80	131.0
81	93.5
82	56.0
83	38.0
84	20.0
85	16.0
86	12.0
87	9.5
88	7.0
89	6.5
90	6.0
91	6.0
92	6.0
93	4.5
94	3.0
95	2.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	542360.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.48334099806127
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.23715489765607	27.44902777855142
2	9.359139648080761	6.829053664588111
3	3.749173772494389	4.103471556087018
4	2.1384105218593574	3.120654410513484
5	1.438012350205942	2.6231747465993447
6	0.9532953315590292	2.0867639191876766
7	0.7440521797529338	1.9001856575993947
8	0.579524068966327	1.6914379379745992
9	0.49505848468985564	1.625524875983115
>10	5.187439540227165	38.887856466252074
>50	0.09318773012034076	2.1449811767770774
>100	0.018036334861979333	1.1778661016734142
>500	0.003507065112051537	0.8684112118327734
>1k	0.002505046508608241	1.8788463158133186
>5k	0.0015030279051649444	3.612744180567199
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCG	6946	1.2806991666052068	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGC	6934	1.2784866140570839	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTC	5885	1.08507264547533	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4075	0.7513459694667749	No Hit
GCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTGC	2352	0.43366029943211154	Illumina Single End Adapter 2 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCT	1418	0.2614499594365366	No Hit
GAACTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCT	1367	0.25204661110701376	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTC	1067	0.19673279740393834	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCTACAGGTCGTA	911	0.16796961427833912	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCG	738	0.13607198170956558	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	687	0.12666863338004278	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGC	683	0.12593111586400177	No Hit
CTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTGCT	597	0.11007448926912014	Illumina Single End Adapter 2 (95% over 22bp)
GAATCTATCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTC	586	0.10804631610000738	No Hit
CCTGTCTCTTATACACATCTGACGCTCTACAGGTCGTATGCCGTCTTCTGC	549	0.10122427907662808	Illumina Single End Adapter 2 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.12482483958994026	0.0
2	0.0	0.0	0.0	0.8741426358876023	0.0
3	0.0	0.0	0.0	1.0898665093295965	0.0
4	0.0	0.0	0.0	1.6308356073456745	0.0
5	0.0	0.0	0.0	3.4696511542149127	0.0
6	0.0	0.0	0.0	3.9636035105833765	0.0
7	0.0	0.0	0.0	4.6402758315509995	0.0
8	0.0	0.0	0.0	5.580795043882293	0.0
9	0.0	0.0	0.0	5.847960764068147	0.0
10	0.0	0.0	0.0	7.965557932000885	0.0
11	0.0	0.0	0.0	8.932074636772624	0.0
12	0.0	0.0	0.0	11.127295523268678	0.0
13	0.0	0.0	0.0	11.483516483516484	0.0
14	1.843793790102515E-4	0.0	0.0	11.668817759421787	0.0
15	1.843793790102515E-4	0.0	0.0	12.10339995574895	0.0
16	1.843793790102515E-4	0.0	0.0	12.673316616269636	0.0
17	1.843793790102515E-4	0.0	0.0	13.377092705951766	0.0
18	1.843793790102515E-4	0.0	0.0	14.112397669444649	0.0
19	1.843793790102515E-4	0.0	0.0	14.64064459030902	0.0
20	1.843793790102515E-4	0.0	0.0	15.099196105907515	0.0
21	1.843793790102515E-4	0.0	0.0	15.66745335201711	0.0
22	1.843793790102515E-4	0.0	0.0	16.249723430931486	0.0
23	1.843793790102515E-4	0.0	0.0	16.850062688988864	0.0
24	1.843793790102515E-4	0.0	0.0	17.3237333136662	0.0
25	1.843793790102515E-4	0.0	0.0	17.754627922413157	0.0
26	1.843793790102515E-4	0.0	0.0	18.152703001696292	0.0
27	1.843793790102515E-4	0.0	0.0	18.576406814661848	0.0
28	1.843793790102515E-4	0.0	0.0	19.020392359318535	0.0
29	1.843793790102515E-4	0.0	0.0	19.48097204808614	0.0
30	1.843793790102515E-4	0.0	0.0	19.993546721734642	0.0
31	3.68758758020503E-4	0.0	0.0	20.465004793863855	0.0
32	3.68758758020503E-4	0.0	0.0	20.898296334537946	0.0
33	3.68758758020503E-4	0.0	0.0	21.343941293605724	0.0
34	3.68758758020503E-4	0.0	0.0	21.80968360498562	0.0
35	3.68758758020503E-4	0.0	0.0	22.27192270816432	0.0
36	3.68758758020503E-4	0.0	0.0	22.72180839294933	0.0
37	3.68758758020503E-4	0.0	0.0	23.19658529390073	0.0
38	3.68758758020503E-4	0.0	0.0	23.649421048749907	0.0
39	3.68758758020503E-4	0.0	0.0	24.132863780514786	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGAATT	25	3.888072E-5	45.0	12
CAGCGCG	20	7.029988E-4	45.0	1
CGACGGT	40	6.8030204E-9	45.0	28
GCGGTCT	20	7.029988E-4	45.0	36
ACGTTAC	20	7.029988E-4	45.0	36
GGCACGT	20	7.029988E-4	45.0	8
CGGATAG	25	3.888072E-5	45.0	2
CGGTCTA	55	1.8189894E-12	45.0	31
CCAACGG	25	3.888072E-5	45.0	2
GATTACC	20	7.029988E-4	45.0	32
CGAATAT	120	0.0	43.124996	14
CGTTTTT	2025	0.0	43.0	1
TACGGCT	805	0.0	42.763973	7
AGGCGAT	100	0.0	42.75	7
CTACGAA	130	0.0	41.53846	11
ACGGCTG	900	0.0	41.5	8
TGATACC	865	0.0	40.578033	4
AAACGGC	95	0.0	40.263157	6
GATACCT	900	0.0	39.0	5
CGGCTGT	960	0.0	38.67187	9
>>END_MODULE