##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936714.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	528640
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.103117433414045	31.0	31.0	33.0	28.0	34.0
2	31.15363006961259	31.0	31.0	34.0	28.0	34.0
3	30.923847987288134	31.0	31.0	34.0	27.0	34.0
4	34.899144975786925	35.0	35.0	37.0	32.0	37.0
5	35.16344960653753	35.0	35.0	37.0	33.0	37.0
6	35.163107218523	36.0	35.0	37.0	32.0	37.0
7	31.002759912227603	35.0	35.0	37.0	0.0	37.0
8	33.08130864104116	36.0	35.0	37.0	17.0	37.0
9	36.32572071731235	39.0	35.0	39.0	32.0	39.0
10	36.155264452179175	37.0	35.0	39.0	32.0	39.0
11	36.426235245157386	38.0	35.0	39.0	32.0	39.0
12	36.48294680690073	38.0	35.0	39.0	32.0	39.0
13	36.41285184624697	38.0	35.0	39.0	32.0	39.0
14	37.47979721549637	39.0	36.0	40.0	32.0	41.0
15	37.67940186138014	39.0	36.0	41.0	33.0	41.0
16	37.557513619854724	39.0	36.0	41.0	33.0	41.0
17	37.48063899818402	39.0	36.0	40.0	32.0	41.0
18	37.39541275726392	39.0	36.0	40.0	32.0	41.0
19	37.17930349576271	39.0	36.0	40.0	32.0	41.0
20	37.16990390435836	39.0	35.0	40.0	32.0	41.0
21	37.15270316283293	39.0	35.0	40.0	32.0	41.0
22	37.24476392251816	39.0	35.0	40.0	32.0	41.0
23	37.311747124697334	39.0	35.0	40.0	32.0	41.0
24	37.22446655569007	39.0	35.0	40.0	32.0	41.0
25	36.88421988498789	39.0	35.0	40.0	31.0	41.0
26	36.847981613196126	39.0	35.0	40.0	31.0	41.0
27	36.84527088377724	39.0	35.0	40.0	31.0	41.0
28	36.82145316283293	39.0	35.0	40.0	31.0	41.0
29	36.84107521186441	39.0	35.0	40.0	31.0	41.0
30	36.58172101997579	38.0	35.0	40.0	31.0	41.0
31	36.470915935230025	38.0	35.0	40.0	30.0	41.0
32	36.33595830811138	38.0	35.0	40.0	30.0	41.0
33	36.175160789951576	38.0	35.0	40.0	30.0	41.0
34	36.06078616828087	38.0	35.0	40.0	30.0	41.0
35	35.98260820217918	38.0	35.0	40.0	29.0	41.0
36	35.89595755145278	38.0	35.0	40.0	29.0	41.0
37	35.79782082324455	38.0	35.0	40.0	29.0	41.0
38	35.71891646489104	38.0	35.0	40.0	29.0	41.0
39	35.71396791767555	38.0	35.0	40.0	28.0	41.0
40	35.59867017251816	38.0	35.0	40.0	28.0	41.0
41	35.445233050847456	38.0	34.0	40.0	27.0	41.0
42	35.42121292372882	38.0	34.0	40.0	27.0	41.0
43	35.3308508625908	38.0	34.0	40.0	27.0	41.0
44	35.095043886198546	38.0	34.0	40.0	26.0	41.0
45	35.00556900726392	38.0	34.0	40.0	25.0	41.0
46	34.84123600181598	38.0	34.0	40.0	24.0	41.0
47	34.80229456719128	37.0	34.0	40.0	24.0	41.0
48	34.73207664951574	37.0	34.0	40.0	24.0	41.0
49	34.60840647699758	37.0	34.0	40.0	24.0	41.0
50	34.43819612590799	37.0	33.0	40.0	24.0	41.0
51	32.8713718220339	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	17.0
9	32.0
10	41.0
11	27.0
12	23.0
13	19.0
14	28.0
15	38.0
16	72.0
17	110.0
18	275.0
19	476.0
20	737.0
21	1207.0
22	1751.0
23	2540.0
24	3806.0
25	5417.0
26	6976.0
27	8080.0
28	9132.0
29	10422.0
30	12316.0
31	15531.0
32	20064.0
33	27916.0
34	43125.0
35	47207.0
36	53703.0
37	79318.0
38	101918.0
39	76311.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.77444007263922	21.667864709443098	23.413097760290558	14.14459745762712
2	33.34707929782083	23.872011198547217	24.94173728813559	17.83917221549637
3	32.16044945520581	22.873600181598064	28.893386803874094	16.072563559322035
4	29.15802814769976	25.107634685230025	27.181446731234864	18.55289043583535
5	24.74273607748184	29.85301906779661	26.07464437046005	19.3296004842615
6	26.11209897094431	34.34095036319613	25.61138014527845	13.935570520581114
7	67.67308565375303	18.351619249394673	10.118606234866828	3.8566888619854724
8	78.18193855932203	5.936743341404358	10.818704600484262	5.0626134987893465
9	71.15976846246973	8.841366525423728	13.66260593220339	6.336259079903147
10	39.98675847457627	25.997843523002423	20.38192342615012	13.633474576271187
11	31.57536319612591	25.480478208232444	25.51112288135593	17.43303571428571
12	30.35317039951574	22.129426452784504	29.360623486682808	18.15677966101695
13	25.067153450363193	25.315337469733656	29.332627118644066	20.284881961259078
14	19.17751210653753	27.845225484261498	30.1089588377724	22.868303571428573
15	18.19026180387409	26.57498486682809	35.769332627118644	19.465420702179177
16	20.933905871670703	24.677285108958838	33.62193553268765	20.766873486682808
17	20.85483504842615	24.37102754237288	30.437537832929785	24.336599576271187
18	23.206529963680385	25.5050696125908	30.567872276029057	20.720528147699756
19	24.747086864406782	27.079865314769975	28.112136803874094	20.060911016949152
20	27.326536016949156	25.31420248184019	28.655796004842614	18.70346549636804
21	24.215912530266344	26.564959140435835	29.15273153753027	20.066396791767556
22	21.40757415254237	25.68477602905569	28.212204903147697	24.695444915254235
23	22.769181295399516	25.90609866828087	29.994325060532688	21.330394975786927
24	23.094544491525422	23.09681446731235	30.685911016949152	23.122730024213077
25	21.567985774818403	26.15201271186441	28.195747578692494	24.084253934624698
26	20.714474878934624	28.33554025423729	28.833799939467315	22.116184927360774
27	21.23013771186441	28.291086561743338	28.965458535108958	21.513317191283292
28	18.269143462469735	29.391078995157383	31.06253783292978	21.277239709443098
29	20.398380750605327	27.594960653753027	29.98921761501211	22.01744098062954
30	21.42838226392252	26.39206265133172	29.55205811138015	22.62749697336562
31	21.6587848062954	29.176566283292978	26.421004842615016	22.74364406779661
32	22.599878934624698	28.06068401937046	27.414119249394673	21.92531779661017
33	21.699644370460046	28.163022094430996	25.909881961259078	24.22745157384988
34	19.940791464891042	28.200665859564168	28.76361985472155	23.094922820823243
35	19.952898002421307	26.080319309927365	28.58485926150121	25.38192342615012
36	19.87988044794189	30.273342917675546	25.99822185230024	23.848554782082324
37	20.039346246973366	29.74406023002421	27.5599651937046	22.65662832929782
38	20.007188256658594	28.037984261501208	26.813899818401936	25.140927663438255
39	21.578578995157386	24.06174334140436	28.21182657384988	26.147851089588375
40	21.840950363196125	24.286849273607746	29.234639830508474	24.63756053268765
41	18.06560230024213	27.239520278450364	27.760479721549636	26.934397699757866
42	20.832513619854723	27.378556295399513	26.425166464891042	25.363763619854723
43	21.8422745157385	26.012976694915253	27.452141343825666	24.692607445520583
44	20.49542221549637	27.346587469733656	27.05243644067797	25.105553874092006
45	21.1537151937046	25.214512711864405	26.844922820823246	26.78684927360775
46	20.508474576271183	27.254653450363197	26.889565677966104	25.347306295399513
47	19.823320217917676	26.717236682808714	28.800696125907994	24.658746973365616
48	19.530304176755447	25.117282082324454	29.003669794188863	26.348743946731233
49	19.9636803874092	24.75805841404358	29.87496216707022	25.403299031477
50	18.51940829297821	25.393651634382568	29.769408292978206	26.317531779661017
51	18.183073547215496	24.48093220338983	27.771451271186443	29.56454297820823
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	185.0
1	580.0
2	975.0
3	4070.5
4	7166.0
5	4862.0
6	2558.0
7	2378.0
8	2198.0
9	2122.0
10	2046.0
11	2013.5
12	1981.0
13	1984.5
14	1988.0
15	1851.5
16	1715.0
17	1708.0
18	1701.0
19	1721.0
20	1741.0
21	2025.5
22	2310.0
23	2365.0
24	2420.0
25	2868.0
26	3823.5
27	4331.0
28	5654.0
29	6977.0
30	8108.0
31	9239.0
32	10386.5
33	11534.0
34	13356.5
35	15179.0
36	16374.5
37	17570.0
38	18755.5
39	19941.0
40	22176.0
41	24411.0
42	27515.0
43	30619.0
44	33189.5
45	35760.0
46	39539.0
47	43318.0
48	45884.5
49	48451.0
50	46583.0
51	44715.0
52	38647.5
53	32580.0
54	29247.5
55	25915.0
56	25230.0
57	24545.0
58	24014.0
59	23483.0
60	23037.5
61	22592.0
62	21297.0
63	20002.0
64	16776.0
65	13550.0
66	10283.5
67	7017.0
68	5872.5
69	4728.0
70	4075.0
71	3422.0
72	2988.5
73	2555.0
74	2110.0
75	1342.5
76	1020.0
77	817.5
78	615.0
79	431.0
80	247.0
81	214.0
82	181.0
83	133.5
84	86.0
85	62.0
86	38.0
87	31.5
88	25.0
89	21.5
90	18.0
91	13.0
92	8.0
93	4.0
94	0.0
95	1.0
96	2.0
97	1.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	528640.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.016732359613826
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.35696883617976	25.293811079724733
2	9.742028557474939	6.627839561586795
3	3.779436539417058	3.856922436944854
4	2.1949656809042857	2.9866224042343443
5	1.3774636179956181	2.3428405614231145
6	1.0103455191151784	2.0621191868685673
7	0.7057658825666611	1.6805494395071725
8	0.5254901511443094	1.4300366263271196
9	0.4361190669931957	1.3351811020948825
>10	5.604687600249804	43.729797234418996
>50	0.2223662115003115	4.711667974534435
>100	0.0415896904302325	2.3903537742533416
>500	0.0011090584114728669	0.2897398514246475
>1k	0.0016635876172093	1.2625187666570088
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4009	0.7583610774818402	No Hit
GCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTGC	1630	0.30833837772397094	TruSeq Adapter, Index 16 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTGC	1054	0.19937953995157387	TruSeq Adapter, Index 16 (95% over 22bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	804	0.15208837772397094	No Hit
CTGTCTCTTATACACATCTGACGCAAATCCCCTCGTATGCCGTCTTCTGCT	732	0.1384685230024213	TruSeq Adapter, Index 19 (95% over 22bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.8916464891041162E-4	0.0	0.0	0.17195066585956417	0.0
2	1.8916464891041162E-4	0.0	0.0	0.9864936440677966	0.0
3	1.8916464891041162E-4	0.0	0.0	1.2528374697336562	0.0
4	1.8916464891041162E-4	0.0	0.0	1.6419491525423728	0.0
5	1.8916464891041162E-4	0.0	0.0	2.7218901331719128	0.0
6	1.8916464891041162E-4	0.0	0.0	3.3035714285714284	0.0
7	1.8916464891041162E-4	0.0	0.0	3.9172215496368037	0.0
8	1.8916464891041162E-4	0.0	0.0	5.023834745762712	0.0
9	1.8916464891041162E-4	0.0	0.0	5.426755447941889	0.0
10	1.8916464891041162E-4	0.0	0.0	6.383739406779661	0.0
11	1.8916464891041162E-4	0.0	0.0	7.632793583535109	0.0
12	1.8916464891041162E-4	0.0	0.0	8.685116525423728	0.0
13	1.8916464891041162E-4	0.0	0.0	9.12681598062954	0.0
14	1.8916464891041162E-4	0.0	0.0	9.302928268765132	0.0
15	1.8916464891041162E-4	0.0	0.0	9.6330205811138	0.0
16	1.8916464891041162E-4	0.0	0.0	10.337280569007264	0.0
17	1.8916464891041162E-4	0.0	0.0	11.149931900726392	0.0
18	1.8916464891041162E-4	0.0	0.0	12.0494098062954	0.0
19	1.8916464891041162E-4	0.0	0.0	12.539724576271187	0.0
20	1.8916464891041162E-4	0.0	0.0	13.029661016949152	0.0
21	1.8916464891041162E-4	0.0	0.0	13.636312046004843	0.0
22	1.8916464891041162E-4	0.0	0.0	14.272283595641646	0.0
23	1.8916464891041162E-4	0.0	0.0	14.888392857142858	0.0
24	1.8916464891041162E-4	0.0	0.0	15.379085956416464	0.0
25	1.8916464891041162E-4	0.0	0.0	15.834216101694915	0.0
26	1.8916464891041162E-4	0.0	0.0	16.250189164648912	0.0
27	1.8916464891041162E-4	0.0	0.0	16.648380750605327	0.0
28	1.8916464891041162E-4	0.0	0.0	17.090458535108958	0.0
29	1.8916464891041162E-4	0.0	0.0	17.534427966101696	0.0
30	1.8916464891041162E-4	0.0	0.0	18.05973819612591	0.0
31	1.8916464891041162E-4	0.0	0.0	18.54381053268765	0.0
32	1.8916464891041162E-4	0.0	0.0	19.011614709443098	0.0
33	1.8916464891041162E-4	0.0	0.0	19.495687046004843	0.0
34	1.8916464891041162E-4	0.0	0.0	19.924334140435835	0.0
35	1.8916464891041162E-4	0.0	0.0	20.430917070217916	0.0
36	1.8916464891041162E-4	0.0	0.0	20.930879237288135	0.0
37	1.8916464891041162E-4	0.0	0.0	21.39263014527845	0.0
38	1.8916464891041162E-4	0.0	0.0	21.831113801452783	0.0
39	1.8916464891041162E-4	0.0	0.0	22.289081416464892	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTAGACG	20	7.0298254E-4	45.0	26
ATGGGCC	55	1.8189894E-12	45.0	5
GTCGACT	20	7.0298254E-4	45.0	43
GCGAATG	25	3.887937E-5	45.0	1
GCGCGTT	25	3.887937E-5	45.0	1
TTAGCGT	20	7.0298254E-4	45.0	14
ACGTTAG	20	7.0298254E-4	45.0	1
ACCGAAC	20	7.0298254E-4	45.0	24
CACGACA	20	7.0298254E-4	45.0	35
AGGCGAT	105	0.0	45.0	7
TCGATAG	25	3.887937E-5	45.0	1
CCCGTGC	20	7.0298254E-4	45.0	44
CAAGGCG	25	3.887937E-5	45.0	5
AACGCAT	20	7.0298254E-4	45.0	22
TTGCGAA	20	7.0298254E-4	45.0	10
TATAGCG	20	7.0298254E-4	45.0	1
CCGGTTG	20	7.0298254E-4	45.0	2
TAGTGCG	25	3.887937E-5	45.0	1
GCTCGAT	20	7.0298254E-4	45.0	12
CGTTTTA	100	0.0	45.0	1
>>END_MODULE