##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936712.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	261056
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.923847756803138	31.0	30.0	33.0	28.0	34.0
2	30.975315641088503	31.0	31.0	33.0	28.0	34.0
3	30.66609463103702	31.0	30.0	33.0	27.0	34.0
4	34.648952715126256	35.0	35.0	37.0	32.0	37.0
5	34.94675854988968	35.0	35.0	37.0	32.0	37.0
6	34.91073562760481	35.0	35.0	37.0	32.0	37.0
7	31.12454798970336	35.0	35.0	37.0	0.0	37.0
8	32.90282544741358	35.0	33.0	37.0	17.0	37.0
9	35.75998253248345	37.0	34.0	39.0	31.0	39.0
10	35.90634193429762	37.0	35.0	39.0	32.0	39.0
11	36.113033218926205	37.0	35.0	39.0	32.0	39.0
12	36.14172821157146	37.0	35.0	39.0	32.0	39.0
13	35.80655491542045	37.0	35.0	39.0	30.0	39.0
14	36.936998191958814	39.0	35.0	40.0	31.0	41.0
15	36.99415451090954	39.0	35.0	40.0	31.0	41.0
16	37.17185584702133	39.0	35.0	40.0	32.0	41.0
17	36.91810952439323	38.0	35.0	40.0	31.0	41.0
18	36.809397217455256	38.0	35.0	40.0	31.0	41.0
19	36.515023596469725	38.0	35.0	40.0	31.0	41.0
20	36.35744821034567	38.0	35.0	40.0	30.0	41.0
21	36.5855180497671	38.0	35.0	40.0	31.0	41.0
22	36.64879183010542	38.0	35.0	40.0	31.0	41.0
23	36.723741266241724	38.0	35.0	40.0	32.0	41.0
24	36.523891425594506	38.0	35.0	40.0	31.0	41.0
25	36.39701826428046	38.0	35.0	40.0	31.0	41.0
26	36.24131987006619	38.0	35.0	40.0	30.0	41.0
27	36.301965861730814	38.0	35.0	40.0	31.0	41.0
28	36.25176207403776	38.0	35.0	40.0	30.0	41.0
29	36.09781426207404	38.0	35.0	40.0	30.0	41.0
30	35.98417963961755	38.0	35.0	40.0	30.0	41.0
31	35.95720841505271	38.0	35.0	40.0	30.0	41.0
32	35.44634101495465	38.0	34.0	40.0	28.0	41.0
33	35.182125674184846	38.0	34.0	40.0	26.0	41.0
34	35.1593029848002	38.0	34.0	40.0	26.0	41.0
35	34.98172039715617	38.0	34.0	40.0	25.0	41.0
36	34.91956898136798	38.0	34.0	40.0	24.0	41.0
37	34.60674721132631	38.0	34.0	40.0	23.0	41.0
38	34.541994821034564	37.0	33.0	40.0	23.0	41.0
39	34.39478502696739	37.0	33.0	40.0	23.0	41.0
40	34.35648290022064	37.0	33.0	40.0	23.0	41.0
41	34.319636399852904	37.0	33.0	40.0	23.0	41.0
42	34.35596960039225	37.0	33.0	40.0	23.0	41.0
43	34.28797269551361	37.0	33.0	40.0	23.0	41.0
44	34.16544726035793	37.0	33.0	40.0	22.0	41.0
45	34.02586801299338	37.0	33.0	40.0	22.0	41.0
46	33.92463302892866	37.0	33.0	40.0	21.0	41.0
47	33.82332909414072	37.0	33.0	40.0	21.0	41.0
48	33.72887426452562	37.0	33.0	40.0	21.0	41.0
49	33.677766456239276	36.0	33.0	40.0	20.0	41.0
50	33.463011767590096	36.0	32.0	40.0	20.0	41.0
51	31.568475729345426	35.0	29.0	38.0	14.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	7.0
9	15.0
10	9.0
11	12.0
12	5.0
13	4.0
14	6.0
15	11.0
16	25.0
17	86.0
18	150.0
19	359.0
20	578.0
21	845.0
22	1275.0
23	1897.0
24	2736.0
25	3849.0
26	4898.0
27	5433.0
28	5731.0
29	6179.0
30	7614.0
31	9219.0
32	12121.0
33	16423.0
34	23882.0
35	26305.0
36	25768.0
37	34553.0
38	42393.0
39	28662.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.713731919588135	16.89982226035793	20.99128156410885	15.395164255945085
2	32.09273106153469	32.52788673694533	21.47546886491787	13.903913336602109
3	25.261246629075757	31.721929394459426	29.965218190733022	13.051605785731796
4	23.17893478793822	20.561105663152734	40.74106705074773	15.518892498161312
5	30.973814047560676	23.67959365040451	28.080565089482718	17.266027212552096
6	21.173234861485657	36.680635572444224	29.351173694532974	12.794955871537143
7	56.91154388330473	16.090800441284628	22.824604682520224	4.173050992890414
8	64.0487098553567	13.260373253248344	16.612144520715862	6.078772370679088
9	60.26599656778622	5.788413214023045	17.383626501593525	16.561963716597205
10	41.08199007109586	19.57166278499632	24.48210345672959	14.86424368717823
11	31.82535547928414	20.551529173817112	30.413780951213532	17.209334395685218
12	25.650052096101987	18.248958077960285	35.17865898504535	20.922330840892375
13	20.02214084334396	23.53786160823731	41.237895317479776	15.202102230938955
14	18.017973155185093	29.03553260603089	31.70699007109586	21.239504167688157
15	13.89127237067909	22.648780338318215	46.642099779357686	16.817847511645013
16	16.494162172101003	22.40017467516548	35.162953542534936	25.942709610198577
17	17.357961510174064	21.636353885756314	36.839605295415545	24.16607930865408
18	17.395118288796276	22.89585376317725	37.3567357195391	22.352292228487375
19	19.162555160578574	24.606214758519247	32.8201611914685	23.411068889433682
20	20.70513606276048	23.673847756803138	38.302126746751654	17.318889433684724
21	18.842700416768814	30.342148810983083	33.29860259867615	17.516548173571955
22	16.80520654572199	21.820988600147096	34.147462613385635	27.226342240745282
23	16.622870188771756	28.910655185094384	33.65829553812209	20.80817908801177
24	21.67389372395195	20.568383795047808	32.65965923020348	25.098063250796766
25	15.708890046580043	31.113630791860754	32.22756803138024	20.949911130178968
26	16.306462981122824	23.295001838685952	34.23709855356705	26.161436626624173
27	21.01732961510174	27.430896052954157	29.45191836234371	22.099855969600394
28	16.03410762441775	22.867890414317234	39.10923326795783	21.988768693307183
29	20.185324221622945	18.986730816376564	34.803260603088994	26.0246843589115
30	20.7978364795293	24.701596592302035	35.787340647217455	18.713226280951215
31	26.734876808041186	20.40788183378279	30.085498896788426	22.771742461387596
32	26.402764157881837	24.386338563373375	31.231996200049032	17.97890107869576
33	21.306156533464087	20.72007538612405	33.38747241971071	24.58629566070115
34	21.665466413336603	20.692112037264035	35.01739090463349	22.62503064476587
35	24.823026477077715	20.744974258396663	30.94661681784751	23.48538244667811
36	25.329048173571955	20.139357072811965	33.161850330963475	21.369744422652612
37	23.467761706300564	21.681937974993872	38.00334027948026	16.8469600392253
38	22.56872088747242	23.778806079921548	31.795093772983574	21.857379259622455
39	20.188388698210346	21.60762441774945	39.64819808776661	18.555788796273596
40	23.876103211571465	20.33471745525864	35.55252512870802	20.23665420446188
41	19.065641088502083	23.32641272370679	34.56576366756558	23.042182520225545
42	23.626348369698455	20.327822382936994	34.53090524638392	21.51492400098063
43	23.44860872762932	20.928842853640596	31.535762441774946	24.086785976955134
44	21.422989703358667	21.535609217945577	33.98083169894582	23.06056937974994
45	19.94514586908556	21.25291125275803	31.652978671242955	27.148964206913462
46	26.207020715861727	24.276017406227016	29.912739029173817	19.604222848737436
47	17.847511645011032	19.99570973277764	41.10612282422162	21.050655797989705
48	21.678873498406475	22.3480785731797	31.43923142927188	24.53381649914195
49	19.544848614856583	17.50735474380976	40.287141456239276	22.660655185094384
50	21.286620495219417	20.19988048541309	34.40947536160824	24.104023657759257
51	20.166554302525128	20.67449129688649	29.68098798725178	29.477966413336603
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	183.0
1	251.0
2	319.0
3	3481.0
4	6643.0
5	4340.0
6	2037.0
7	1945.0
8	1853.0
9	1853.0
10	1853.0
11	1806.0
12	1759.0
13	1645.0
14	1531.0
15	1413.5
16	1296.0
17	1277.0
18	1258.0
19	1171.5
20	1085.0
21	1071.0
22	1057.0
23	1120.5
24	1184.0
25	1455.0
26	2187.0
27	2648.0
28	2555.5
29	2463.0
30	3054.5
31	3646.0
32	3989.0
33	4332.0
34	4895.0
35	5458.0
36	6021.5
37	6585.0
38	6945.5
39	7306.0
40	9017.5
41	10729.0
42	12459.0
43	14189.0
44	16991.0
45	19793.0
46	34834.5
47	49876.0
48	36989.0
49	24102.0
50	24201.0
51	24300.0
52	19838.0
53	15376.0
54	13391.0
55	11406.0
56	9748.0
57	8090.0
58	7633.0
59	7176.0
60	6718.5
61	6261.0
62	5455.5
63	4650.0
64	3795.0
65	2940.0
66	2553.5
67	2167.0
68	1873.5
69	1580.0
70	1278.0
71	976.0
72	749.0
73	522.0
74	386.5
75	223.5
76	196.0
77	168.5
78	141.0
79	91.5
80	42.0
81	31.5
82	21.0
83	20.0
84	19.0
85	12.0
86	5.0
87	5.0
88	5.0
89	3.0
90	1.0
91	3.0
92	5.0
93	7.5
94	10.0
95	7.5
96	5.0
97	2.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	261056.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.36159291493013
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.01080231430387	26.462138391762686
2	9.344169091334738	6.421610688894337
3	3.4803741234964267	3.58773596469723
4	1.906290759506371	2.6201274822260356
5	1.2162358003634215	2.0895899730326057
6	0.8004191610091079	1.6502206423142929
7	0.5819203371124712	1.399699681294435
8	0.44703075705383327	1.2288551115469477
9	0.328863025762795	1.0170231674430006
>10	4.7501198399161675	35.62530644765874
>50	0.09141277326287861	1.9336847266486885
>100	0.02564016811031961	1.8417504290267221
>500	0.008918319342719864	2.3224901936749203
>1k	0.004459159671359932	3.6057397646481983
>5k	0.0033443697535199494	8.19402733513116
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCG	7656	2.9327040941407208	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGC	7463	2.858773596469723	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTC	6272	2.402549644520716	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3985	1.5264924000980633	No Hit
GCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTCTGC	2650	1.0151078695758764	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCT	1652	0.6328144152978671	No Hit
GAACTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTCT	1126	0.4313250796763913	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTA	988	0.3784628585437607	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTC	987	0.37807979897033583	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCG	843	0.32291922039715615	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGC	784	0.3003187055650895	No Hit
GAATCTATCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTC	751	0.28767773964206916	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTT	659	0.2524362588869821	No Hit
CCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTCTGC	539	0.20646911007599902	No Hit
CTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTCTGCT	512	0.19612650159352782	Illumina Single End Adapter 1 (95% over 21bp)
GATATGGGGAGATCATGTGTTTAATAAATTGGCTATAATTTTAATATCTGT	430	0.16471561657268938	No Hit
GAATGATACGGCGACCACCTGTCTCTTATACACATCTGACGCCGGTTTGTT	336	0.12870801667075263	No Hit
ATGCAAGGGATGGTTTTATTTTCTACAATTTTTGTGTATTTGCTTTTTTGA	306	0.11721622946800686	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	293	0.1122364550134837	No Hit
TCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTATGCCGTCTTCTGC	293	0.1122364550134837	No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGT	288	0.11032115714635941	No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCCGGTTTGTTCGTAT	286	0.10955503799950969	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2233237313066928	0.0
2	0.0	0.0	0.0	1.8348553567050747	0.0
3	0.0	0.0	0.0	2.1485811473400345	0.0
4	0.0	0.0	0.0	3.005868472664869	0.0
5	0.0	0.0	0.0	6.560278254474136	0.0
6	0.0	0.0	0.0	7.324865163030155	0.0
7	0.0	0.0	0.0	8.53609953419956	0.0
8	0.0	0.0	0.0	9.927754964452072	0.0
9	0.0	0.0	0.0	10.316943491051727	0.0
10	0.0	0.0	0.0	14.562009683746016	0.0
11	0.0	0.0	0.0	15.768647340034322	0.0
12	0.0	0.0	0.0	20.119437974993872	0.0
13	0.0	0.0	0.0	20.703220764893356	0.0
14	0.0	0.0	0.0	21.01005148320667	0.0
15	0.0	0.0	0.0	21.795706668301055	0.0
16	0.0	0.0	0.0	22.567571708752144	0.0
17	0.0	0.0	0.0	23.386553076734494	0.0
18	0.0	0.0	0.0	24.26835621475852	0.0
19	0.0	0.0	0.0	24.97701642559451	0.0
20	0.0	0.0	0.0	25.45813924981613	0.0
21	0.0	0.0	0.0	26.055329124785487	0.0
22	0.0	0.0	0.0	26.680865408188282	0.0
23	0.0	0.0	0.0	27.28380117675901	0.0
24	0.0	0.0	0.0	27.778714145623926	0.0
25	0.0	0.0	0.0	28.19203542534935	0.0
26	0.0	0.0	0.0	28.61071953910272	0.0
27	0.0	0.0	0.0	29.043193797499388	0.0
28	0.0	0.0	0.0	29.451535302770285	0.0
29	0.0	0.0	0.0	29.876348369698455	0.0
30	0.0	0.0	0.0	30.31648381956362	0.0
31	0.0	0.0	0.0	30.74551054179946	0.0
32	0.0	0.0	0.0	31.15538428536406	0.0
33	0.0	0.0	0.0	31.564491909781808	0.0
34	0.0	0.0	0.0	31.96670446187791	0.0
35	0.0	0.0	0.0	32.409904388330474	0.0
36	0.0	0.0	0.0	32.81671365530767	0.0
37	0.0	0.0	0.0	33.211648075508705	0.0
38	0.0	0.0	0.0	33.59202623191959	0.0
39	0.0	0.0	0.0	34.00496445207158	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGACC	20	7.0232595E-4	45.000004	10
CGTTATA	20	7.0232595E-4	45.000004	15
GTTACCG	45	3.8198777E-10	45.000004	10
TGACCAC	20	7.0232595E-4	45.000004	36
TCAGCGA	25	3.8824965E-5	45.000004	26
CGTAGCG	25	3.8824965E-5	45.000004	2
GCGATTT	20	7.0232595E-4	45.000004	9
CCCTTTG	20	7.0232595E-4	45.000004	18
TAGATTC	25	3.8824965E-5	45.000004	20
AACCTCC	20	7.0232595E-4	45.000004	26
CGAGAAT	40	6.7793735E-9	45.000004	6
GCGGATG	25	3.8824965E-5	45.000004	5
GACGCAT	20	7.0232595E-4	45.000004	10
ATACGGT	40	6.7793735E-9	45.000004	28
CTCTCGA	20	7.0232595E-4	45.000004	11
TTCGCGG	20	7.0232595E-4	45.000004	2
AGTACTT	20	7.0232595E-4	45.000004	42
AGTGTGC	20	7.0232595E-4	45.000004	28
CGAAGTA	45	3.8198777E-10	45.000004	15
GCGACCT	75	0.0	45.0	11
>>END_MODULE