##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936710.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	361263
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.16108762868049	31.0	31.0	33.0	28.0	34.0
2	31.19196817830777	31.0	31.0	34.0	28.0	34.0
3	30.92816590683242	31.0	31.0	34.0	27.0	34.0
4	34.888200563024725	35.0	35.0	37.0	32.0	37.0
5	35.173530087498584	35.0	35.0	37.0	33.0	37.0
6	35.16427644126301	36.0	35.0	37.0	32.0	37.0
7	31.031007880685262	35.0	35.0	37.0	0.0	37.0
8	33.11723038340489	36.0	35.0	37.0	17.0	37.0
9	36.455800898514376	39.0	35.0	39.0	32.0	39.0
10	36.124718003227564	37.0	35.0	39.0	32.0	39.0
11	36.403163346370924	38.0	35.0	39.0	32.0	39.0
12	36.43933643910392	38.0	35.0	39.0	32.0	39.0
13	36.358096456044485	39.0	35.0	39.0	32.0	39.0
14	37.39792616459477	39.0	36.0	41.0	32.0	41.0
15	37.64696080141061	39.0	36.0	41.0	33.0	41.0
16	37.58502808203442	39.0	36.0	41.0	33.0	41.0
17	37.524418498434656	39.0	36.0	41.0	33.0	41.0
18	37.41451241893025	39.0	36.0	40.0	32.0	41.0
19	37.15879013350385	39.0	36.0	40.0	32.0	41.0
20	37.142757492463936	39.0	35.0	40.0	32.0	41.0
21	37.110639617121045	39.0	35.0	40.0	32.0	41.0
22	37.160951993423076	39.0	35.0	40.0	32.0	41.0
23	37.20198581089124	39.0	35.0	40.0	32.0	41.0
24	37.126652881695605	39.0	35.0	40.0	32.0	41.0
25	36.82582772107855	39.0	35.0	40.0	31.0	41.0
26	36.79540390242012	39.0	35.0	40.0	31.0	41.0
27	36.80434752521017	39.0	35.0	40.0	31.0	41.0
28	36.846485801203	39.0	35.0	40.0	31.0	41.0
29	36.82727265178001	39.0	35.0	40.0	31.0	41.0
30	36.57808577130788	38.0	35.0	40.0	31.0	41.0
31	36.50963425537628	38.0	35.0	40.0	31.0	41.0
32	36.310729302474925	38.0	35.0	40.0	30.0	41.0
33	36.11811616467781	38.0	35.0	40.0	30.0	41.0
34	36.00753744501928	38.0	35.0	40.0	29.0	41.0
35	35.885595812469035	38.0	35.0	40.0	29.0	41.0
36	35.810794352037156	38.0	35.0	40.0	29.0	41.0
37	35.71996578669833	38.0	35.0	40.0	28.0	41.0
38	35.58869853818409	38.0	35.0	40.0	27.0	41.0
39	35.52701494479091	38.0	35.0	40.0	27.0	41.0
40	35.42082361049983	38.0	35.0	40.0	26.0	41.0
41	35.30645540783308	38.0	34.0	40.0	26.0	41.0
42	35.30562498788971	38.0	34.0	40.0	26.0	41.0
43	35.23080691905897	38.0	34.0	40.0	26.0	41.0
44	34.991017624279266	38.0	34.0	40.0	24.0	41.0
45	34.923346703094424	38.0	34.0	40.0	24.0	41.0
46	34.8131416724104	38.0	34.0	40.0	24.0	41.0
47	34.79859548306912	38.0	34.0	40.0	24.0	41.0
48	34.71542892574108	38.0	34.0	40.0	23.0	41.0
49	34.619429612221566	37.0	34.0	40.0	24.0	41.0
50	34.46635276792807	37.0	34.0	40.0	23.0	41.0
51	32.85428067640473	35.0	31.0	39.0	19.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	19.0
9	36.0
10	35.0
11	28.0
12	20.0
13	12.0
14	16.0
15	41.0
16	72.0
17	113.0
18	202.0
19	352.0
20	558.0
21	918.0
22	1321.0
23	2113.0
24	3042.0
25	4317.0
26	5561.0
27	5869.0
28	6231.0
29	6920.0
30	8232.0
31	10022.0
32	13067.0
33	18408.0
34	28712.0
35	31878.0
36	34631.0
37	52082.0
38	70542.0
39	55893.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.53850795680709	20.963121050315145	24.80741177480118	14.690959218076582
2	35.464744521304425	23.785718437814	25.25362409103617	15.495912949845403
3	33.04047189997315	22.360717814999045	29.086288936315096	15.51252134871271
4	28.42306020821396	25.767100422683754	27.69838040430379	18.111458964798498
5	25.37846388918876	29.03895499954327	27.401920484522357	18.180660626745613
6	25.590497781394717	33.87476713640755	26.88484566645353	13.649889415744209
7	68.21069414803065	18.226610530278496	10.355890307061614	3.2068050146292313
8	79.62952198259994	5.801867337645981	10.941613173781981	3.6269975059721036
9	73.73907651766164	7.778543609503326	13.092400827098263	5.389979045736762
10	40.044233702316596	25.430503538972992	21.215291906450425	13.309970852259989
11	31.966185299906165	26.001832460008362	25.707863800057023	16.324118440028457
12	29.097638008874423	23.39652829102344	29.935254925082283	17.57057877501986
13	23.822533721969865	26.04363026382441	30.711697572128895	19.422138442076825
14	19.726348947996335	29.493748321859698	31.05770588186446	19.72219684827951
15	19.34269493416154	26.775507040577086	36.373223939346126	17.508574085915246
16	21.81706955874252	24.063078698898032	35.2039926590877	18.915859083271744
17	22.26494271486424	23.985572837517267	31.239567849461476	22.50991659815702
18	23.955400912908324	24.82457378696407	30.949197675931384	20.270827624196222
19	24.229439494218894	26.31351674541816	29.851659317450167	19.605384442912783
20	26.621879351054496	25.775681428765196	29.557967464146618	18.04447175603369
21	24.692813822616763	25.763778742910286	30.277387941748806	19.266019492724137
22	23.723990555357176	23.858241779534577	30.854806609035524	21.562961056072723
23	21.995609846566076	26.59918120593584	30.213999219405252	21.19120972809283
24	22.612888671134325	24.621120900839554	32.812660028843254	19.953330399182867
25	20.860702590633416	25.423029759482702	30.638343810464953	23.077923839418926
26	20.433589933095835	28.103348530018295	30.380083208078325	21.082978328807545
27	20.648115085131884	28.280781591250697	31.28164245992532	19.789460863692103
28	19.316951915917212	27.151687274921592	33.04323996645103	20.48812084271016
29	21.07301328948716	25.9337380246524	32.177388772168754	20.815859913691686
30	20.80478764778015	25.999064393530475	31.853248187608475	21.3428997710809
31	22.375111760684042	27.278464719608706	29.156044211557784	21.190379308149467
32	23.512787083094587	26.8328060166693	29.504820587771235	20.14958631246488
33	22.209304578658763	27.039026969271696	29.671458189739887	21.08021026232966
34	21.131419492170526	26.041692617289897	31.162338794728495	21.664549095811083
35	21.345391030911	25.919067272319612	30.793909146522065	21.941632550247327
36	21.915335918707424	27.726891489026002	29.70024608110988	20.657526511156693
37	21.425664958769648	27.218397677038613	30.833492497155813	20.522444867035926
38	21.583167941361282	27.969097305840897	28.351644093084538	22.096090659713283
39	22.881391119489123	26.097053946847588	30.019957759305548	21.001597174357737
40	22.516006344408368	26.087919327470573	30.917364911435712	20.47870941668535
41	19.751815159592873	25.757412190011152	31.320118583967915	23.17065406642806
42	22.46202904808962	26.002939686599515	29.299706861759994	22.235324403550877
43	22.9395205155247	25.974982215172883	28.677445517531545	22.40805175177087
44	21.248231897537252	27.213415157378417	28.7912130497726	22.747139895311726
45	21.6977658935457	26.615789604803147	28.783462463634528	22.902982038016624
46	21.955749689284538	26.72734268386189	29.26925813050326	22.047649496350306
47	21.223872912531867	26.054702529735955	30.70145572616072	22.01996883157146
48	21.586489621134742	25.59963240077174	30.670453381608414	22.143424596485108
49	21.870216435117907	24.458358591939945	30.974387080880135	22.697037892062017
50	19.97575173765373	25.907995006408076	30.64332633012514	23.472926925813052
51	20.135745980075455	26.11061747258922	29.09542355569211	24.658212991643207
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	126.0
1	390.5
2	655.0
3	2982.5
4	5310.0
5	3674.5
6	2039.0
7	1980.5
8	1922.0
9	1875.0
10	1828.0
11	1743.0
12	1658.0
13	1709.5
14	1761.0
15	1757.5
16	1754.0
17	1715.0
18	1676.0
19	1614.5
20	1553.0
21	1666.0
22	1779.0
23	2051.5
24	2324.0
25	2262.0
26	2749.0
27	3298.0
28	4058.5
29	4819.0
30	6134.5
31	7450.0
32	8176.5
33	8903.0
34	10317.5
35	11732.0
36	11875.0
37	12018.0
38	13126.0
39	14234.0
40	16188.5
41	18143.0
42	20291.5
43	22440.0
44	24767.0
45	27094.0
46	28991.0
47	30888.0
48	30916.5
49	30945.0
50	29788.5
51	28632.0
52	25996.5
53	23361.0
54	21401.5
55	19442.0
56	17645.5
57	15849.0
58	14981.0
59	14113.0
60	13283.0
61	12453.0
62	10984.5
63	9516.0
64	7780.0
65	6044.0
66	5281.5
67	4519.0
68	3768.0
69	3017.0
70	2654.0
71	2291.0
72	1819.0
73	1347.0
74	1185.5
75	758.0
76	492.0
77	412.5
78	333.0
79	231.5
80	130.0
81	98.5
82	67.0
83	45.0
84	23.0
85	22.0
86	21.0
87	15.0
88	9.0
89	10.0
90	11.0
91	10.5
92	10.0
93	7.5
94	5.0
95	4.5
96	4.0
97	2.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	361263.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.174920430415895
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.20377378852662	24.94879211501367
2	8.516643961905809	5.6507797154081425
3	3.4230720270367088	3.4068042637357583
4	2.026024029338267	2.688527438536304
5	1.4086264443821837	2.3365535104279305
6	1.0055714672628284	2.0015852048124536
7	0.8117462753683287	1.8850732666521541
8	0.6012839529831722	1.5958037837042134
9	0.4902117762698976	1.4636463004626021
>10	6.296752013402972	47.98749631856075
>50	0.19458132587224913	3.8232633494498853
>100	0.02004271167782824	1.243391732777615
>500	8.351129865761765E-4	0.15154529363399183
>1k	8.351129865761765E-4	0.8167377068245117
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2948	0.8160259976803603	No Hit
GCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTCTGC	547	0.15141323634028395	TruSeq Adapter, Index 20 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCCTCGTTTGTCGTATGCCGTCTTCTGC	369	0.10214165303393927	TruSeq Adapter, Index 20 (95% over 21bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.10186484638615081	0.0
2	0.0	0.0	0.0	0.5134763316475809	0.0
3	0.0	0.0	0.0	0.660737468271038	0.0
4	0.0	0.0	0.0	0.8879957261053582	0.0
5	0.0	0.0	0.0	1.5069353905603398	0.0
6	0.0	0.0	0.0	1.869275292515425	0.0
7	0.0	0.0	0.0	2.2263558681625297	0.0
8	0.0	0.0	0.0	2.7965775626067435	0.0
9	0.0	0.0	0.0	3.000584062026834	0.0
10	0.0	0.0	0.0	3.58464608886047	0.0
11	0.0	0.0	0.0	4.365240835623909	0.0
12	0.0	0.0	0.0	5.0364969565109075	0.0
13	0.0	0.0	0.0	5.298079238670996	0.0
14	0.0	0.0	0.0	5.4082482844908	0.0
15	0.0	0.0	0.0	5.638274608803005	0.0
16	0.0	0.0	0.0	6.116319689533664	0.0
17	0.0	0.0	0.0	6.682389284261051	0.0
18	0.0	0.0	0.0	7.350600532022377	0.0
19	0.0	0.0	0.0	7.726780766366885	0.0
20	0.0	0.0	0.0	8.09105831485649	0.0
21	0.0	0.0	0.0	8.52038542557638	0.0
22	0.0	0.0	0.0	8.995385633181366	0.0
23	0.0	0.0	0.0	9.468171387604045	0.0
24	0.0	0.0	0.0	9.865112120532686	0.0
25	0.0	0.0	0.0	10.210843623620464	0.0
26	0.0	0.0	0.0	10.509794803231994	0.0
27	0.0	0.0	0.0	10.84334681381708	0.0
28	0.0	0.0	0.0	11.14949496627111	0.0
29	0.0	0.0	0.0	11.470590677705715	0.0
30	0.0	0.0	0.0	11.894381655469838	0.0
31	0.0	0.0	0.0	12.269177856575403	0.0
32	0.0	0.0	0.0	12.631517758530489	0.0
33	0.0	0.0	0.0	13.017663032195381	0.0
34	0.0	0.0	0.0	13.34346445664239	0.0
35	0.0	0.0	0.0	13.709679651666514	0.0
36	0.0	0.0	0.0	14.04683014867285	0.0
37	2.768066477884533E-4	0.0	0.0	14.396160138181878	0.0
38	2.768066477884533E-4	0.0	0.0	14.741614834621869	0.0
39	2.768066477884533E-4	0.0	0.0	15.095373730495512	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTGA	25	3.8854778E-5	45.0	6
TCGTTGA	20	7.0268573E-4	45.0	24
CCGATAA	20	7.0268573E-4	45.0	11
GCGCGCC	25	3.8854778E-5	45.0	16
CGGTTTA	20	7.0268573E-4	45.0	1
GACCGTT	25	3.8854778E-5	45.0	39
TCGATAG	20	7.0268573E-4	45.0	2
TAGCCGT	25	3.8854778E-5	45.0	44
CTGCGCG	20	7.0268573E-4	45.0	1
ATCCGGC	20	7.0268573E-4	45.0	18
TACGCGG	20	7.0268573E-4	45.0	2
TGCGCGC	25	3.8854778E-5	45.0	15
TACGATG	25	3.8854778E-5	45.0	1
TACGACC	20	7.0268573E-4	45.0	32
TCGTACA	20	7.0268573E-4	45.0	34
TAGATCG	20	7.0268573E-4	45.0	1
TATAGCG	20	7.0268573E-4	45.0	1
CGTAAAG	20	7.0268573E-4	45.0	28
ACGTACT	20	7.0268573E-4	45.0	30
CGCACGG	25	3.8854778E-5	45.0	2
>>END_MODULE