##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2936708.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 357990 Sequences flagged as poor quality 0 Sequence length 51 %GC 43 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.09659487695187 31.0 31.0 33.0 28.0 34.0 2 31.157222268778458 31.0 31.0 34.0 28.0 34.0 3 30.779370932148943 31.0 30.0 34.0 27.0 34.0 4 34.75048465040923 35.0 35.0 37.0 32.0 37.0 5 35.111184669962846 35.0 35.0 37.0 33.0 37.0 6 35.13636973099807 36.0 35.0 37.0 32.0 37.0 7 31.090536048492975 35.0 35.0 37.0 0.0 37.0 8 33.1854912148384 37.0 35.0 37.0 17.0 37.0 9 36.47380373753457 39.0 35.0 39.0 32.0 39.0 10 36.240847509706974 37.0 35.0 39.0 32.0 39.0 11 36.43983630827677 38.0 35.0 39.0 32.0 39.0 12 36.56436771976871 38.0 35.0 39.0 32.0 39.0 13 36.506575602670466 39.0 35.0 39.0 32.0 39.0 14 37.560688846057154 39.0 36.0 41.0 32.0 41.0 15 37.821126288443814 40.0 37.0 41.0 33.0 41.0 16 37.737950780748065 39.0 36.0 41.0 33.0 41.0 17 37.6621777144613 39.0 36.0 41.0 33.0 41.0 18 37.484602921869325 39.0 36.0 40.0 33.0 41.0 19 37.18580127936534 39.0 36.0 40.0 32.0 41.0 20 37.0824604039219 39.0 35.0 40.0 32.0 41.0 21 37.067932065141484 39.0 35.0 40.0 32.0 41.0 22 37.08364200117322 39.0 35.0 40.0 32.0 41.0 23 37.132791418754714 39.0 35.0 40.0 32.0 41.0 24 37.05887315288137 39.0 35.0 40.0 32.0 41.0 25 36.75060476549624 38.0 35.0 40.0 31.0 41.0 26 36.73233609877371 38.0 35.0 40.0 31.0 41.0 27 36.77533171317634 38.0 35.0 40.0 32.0 41.0 28 36.80243582222967 39.0 35.0 40.0 32.0 41.0 29 36.76310511466801 39.0 35.0 40.0 31.0 41.0 30 36.49053884186709 38.0 35.0 40.0 31.0 41.0 31 36.36978686555491 38.0 35.0 40.0 30.0 41.0 32 36.082396156317216 38.0 35.0 40.0 30.0 41.0 33 35.78282633593117 38.0 35.0 40.0 29.0 41.0 34 35.574560741920166 38.0 35.0 40.0 27.0 41.0 35 35.41290259504456 38.0 35.0 40.0 25.0 41.0 36 35.31724629179586 38.0 35.0 40.0 25.0 41.0 37 35.2077097125618 38.0 35.0 40.0 24.0 41.0 38 35.10650297494343 38.0 35.0 40.0 24.0 41.0 39 35.033595910500296 38.0 34.0 40.0 23.0 41.0 40 34.95887315288137 38.0 34.0 40.0 23.0 41.0 41 34.85669990781865 38.0 34.0 40.0 23.0 41.0 42 34.83372440570966 38.0 34.0 40.0 23.0 41.0 43 34.77049917595463 38.0 34.0 40.0 23.0 41.0 44 34.51630213134445 38.0 34.0 40.0 21.0 41.0 45 34.44830023185005 38.0 34.0 40.0 22.0 41.0 46 34.32911254504316 38.0 34.0 40.0 21.0 41.0 47 34.32238889354451 38.0 33.0 40.0 21.0 41.0 48 34.306304645381154 38.0 33.0 40.0 21.0 41.0 49 34.219112265705746 38.0 33.0 40.0 20.0 41.0 50 34.06049330986899 37.0 33.0 40.0 20.0 41.0 51 32.48863096734546 35.0 31.0 39.0 17.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 10.0 9 23.0 10 32.0 11 14.0 12 12.0 13 8.0 14 12.0 15 20.0 16 45.0 17 92.0 18 199.0 19 357.0 20 619.0 21 1035.0 22 1512.0 23 2407.0 24 3578.0 25 5439.0 26 6843.0 27 7341.0 28 7271.0 29 7545.0 30 8256.0 31 9898.0 32 12631.0 33 17177.0 34 26583.0 35 30556.0 36 33837.0 37 50405.0 38 69095.0 39 55137.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 32.50398055811615 22.930528785720274 26.675046789016456 17.890443867147127 2 37.04600687170033 23.412944495656305 24.097879829045503 15.443168803597866 3 29.395513841168746 21.703399536299898 33.637811112042236 15.26327551048912 4 28.07089583507919 23.26098494371351 31.078801083829156 17.58931813737814 5 23.333612670744994 27.687644906282298 31.553116008827065 17.425626414145647 6 24.96159110589681 31.79530154473589 30.09134333361267 13.15176401575463 7 65.2364591189698 17.997709433224394 13.712394200955332 3.053437246850471 8 76.97673119360876 4.715494846224755 14.446772256208273 3.861001703958211 9 70.89779044107377 6.976731193608759 16.4378893265175 5.687589038799967 10 36.60213972457331 29.88351629933797 22.83220201681611 10.682141959272604 11 25.23338640744155 24.912148384033074 34.2836392077991 15.570826000726276 12 24.9699712282466 21.27796865834241 37.0027095728931 16.74935054051789 13 21.65116344031956 24.20319003324115 37.592111511494736 16.55353501494455 14 16.785105729210315 28.596329506410793 35.534791474622196 19.083773289756696 15 16.54878627894634 25.077236794323866 40.22039721779938 18.15357970893042 16 19.108075644571077 25.5306014134473 37.7373669655577 17.62395597642392 17 19.996927288471745 23.72775775859661 35.26746557166401 21.007849381267633 18 21.14276935109919 24.145367189027628 35.31886365540937 19.392999804463813 19 21.422665437582054 26.01525182267661 33.373557920612306 19.18852481912903 20 22.028548283471604 25.338417274225538 35.295399312829964 17.33763512947289 21 21.93273555127238 24.29006396826727 36.090114249001374 17.687086231458977 22 19.319254727785694 23.129137685410207 35.68395765244839 21.86764993435571 23 18.08542138048549 25.468309170647224 35.66859409480712 20.777675354060168 24 19.572334422749236 24.017989329310872 36.603257074219954 19.806419173719934 25 18.74074694823878 25.472499231822116 35.61943071035504 20.167323109584068 26 18.303025224168273 27.622279951953963 34.830581859828484 19.244112964049275 27 18.524260454202633 26.85521941953686 35.06410793597586 19.556412190284643 28 16.953546188441017 25.837593228861138 37.47171708706947 19.73714349562837 29 19.02064303472164 24.66772814883097 36.390681303947034 19.92094751250035 30 19.126232576328945 26.024749294673033 33.39813961283834 21.45087851615967 31 19.942456493198137 27.182044191178523 32.21961507304673 20.655884242576608 32 19.92793094779184 27.830386323640326 31.448085142043077 20.793597586524765 33 20.118439062543647 27.48596329506411 31.11846699628481 21.277130646107434 34 18.816168049386857 26.930081845861615 32.4117992122685 21.84195089248303 35 17.861392776334533 26.883432498114473 32.23022989468979 23.024944830861198 36 19.342160395541775 28.363362105086736 30.462303416296542 21.832174083074946 37 18.585994022179392 27.628146037598817 33.03053157909439 20.755328361127408 38 19.782116818905557 27.82982764881701 30.708120338556945 21.679935193720496 39 19.847481773233888 27.378697728986843 31.16260230732702 21.61121819045225 40 20.26453252884159 26.47532053967988 32.31403111818766 20.946115813290874 41 17.956646833710437 27.116679236850196 31.517919494957958 23.40875443448141 42 19.475683678315036 26.790133802620186 30.208106371686362 23.52607614737842 43 19.917036788737118 26.245984524707396 31.118187658873154 22.718791027682336 44 20.063968267270035 27.304114640073745 30.330176820581578 22.30174027207464 45 20.87376742367105 27.554121623509037 29.249699712282467 22.322411240537445 46 19.782396156317215 28.05525294002626 29.348026481186622 22.814324422469902 47 17.990725997932902 26.750188552752867 33.21740830749462 22.041677141819605 48 18.897175898768122 25.640101678817846 31.70116483700662 23.761557585407413 49 19.558646889577922 24.573032766278388 33.32188049945529 22.546439844688397 50 18.634319394396492 24.643146456604935 33.147573954579734 23.57496019441884 51 17.285399033492553 25.37920053632783 31.521550881309533 25.81384954887008 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 174.0 1 333.5 2 493.0 3 5180.0 4 9867.0 5 6707.5 6 3548.0 7 3344.5 8 3141.0 9 3070.0 10 2999.0 11 2915.5 12 2832.0 13 2755.0 14 2678.0 15 2479.5 16 2281.0 17 2168.5 18 2056.0 19 2063.0 20 2070.0 21 2023.5 22 1977.0 23 2376.5 24 2776.0 25 3219.0 26 4055.0 27 4448.0 28 5615.0 29 6782.0 30 7237.5 31 7693.0 32 8580.5 33 9468.0 34 10724.5 35 11981.0 36 12521.5 37 13062.0 38 13732.5 39 14403.0 40 16103.5 41 17804.0 42 20722.5 43 23641.0 44 25517.0 45 27393.0 46 29825.5 47 32258.0 48 34378.5 49 36499.0 50 32905.0 51 29311.0 52 24798.5 53 20286.0 54 18163.5 55 16041.0 56 13937.0 57 11833.0 58 10538.5 59 9244.0 60 8763.5 61 8283.0 62 7528.5 63 6774.0 64 5334.5 65 3895.0 66 3196.0 67 2497.0 68 2043.5 69 1590.0 70 1242.0 71 894.0 72 813.5 73 733.0 74 515.0 75 223.5 76 150.0 77 105.5 78 61.0 79 55.5 80 50.0 81 35.5 82 21.0 83 15.0 84 9.0 85 17.5 86 26.0 87 15.5 88 5.0 89 4.5 90 4.0 91 2.0 92 0.0 93 0.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 357990.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 36.75425499292695 #Duplication Level Percentage of deduplicated Percentage of total 1 75.98256453337775 27.92682551876294 2 9.336885021287435 6.863405058240772 3 3.579116008907212 3.9464322732192803 4 2.0329127730786167 2.988727777604389 5 1.2784238988137335 2.3493758983025903 6 0.8813410554066154 1.9435820331690064 7 0.6661379472197158 1.7138382780804844 8 0.49458790184490886 1.4542567888659608 9 0.40353856608405125 1.334858342159999 >10 5.158656381312728 41.654843694378926 >50 0.15397832763858427 3.447190667035048 >100 0.03034055717016439 2.2484102490608704 >500 0.0 0.0 >1k 7.585139292541098E-4 0.5940932722565052 >5k 7.585139292541098E-4 1.5341601488632326 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 5503 1.5371937763624683 No Hit TATGAAAACTATGCCCTCGTGTACTCCTGCACCACCTTCTTCTGGCTCTTC 2131 0.5952680242464873 No Hit CCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTCTTCTGC 498 0.1391100310064527 No Hit GCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTCTTCTGC 473 0.1321265957149641 No Hit GGCAAGGTGGGGTGAGGCCCGGAGTTGGGGAAGCTAGGAGGCTTAAAGCCT 464 0.12961255901002822 No Hit CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 414 0.11564568842705103 No Hit TCTGTCTCTTATACACATCTGACGCACCGATCATCGTATGCCGTCTTCTGC 375 0.10475152937232883 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 2.793374116595436E-4 0.0 0.0 0.08128718679292718 0.0 2 2.793374116595436E-4 0.0 0.0 0.5896812760132965 0.0 3 2.793374116595436E-4 0.0 0.0 0.8469510321517361 0.0 4 2.793374116595436E-4 0.0 0.0 1.2455655185899048 0.0 5 2.793374116595436E-4 0.0 0.0 2.25844297326741 0.0 6 2.793374116595436E-4 0.0 0.0 2.765440375429481 0.0 7 2.793374116595436E-4 0.0 0.0 3.267409704181681 0.0 8 2.793374116595436E-4 0.0 0.0 4.164641470432135 0.0 9 2.793374116595436E-4 0.0 0.0 4.506550462303417 0.0 10 2.793374116595436E-4 0.0 0.0 5.278359730718735 0.0 11 2.793374116595436E-4 0.0 0.0 6.513869102488896 0.0 12 2.793374116595436E-4 0.0 0.0 7.45411883013492 0.0 13 2.793374116595436E-4 0.0 0.0 7.8348557222268775 0.0 14 2.793374116595436E-4 0.0 0.0 7.973965753233331 0.0 15 2.793374116595436E-4 0.0 0.0 8.272577446297383 0.0 16 2.793374116595436E-4 0.0 0.0 8.893265175004888 0.0 17 2.793374116595436E-4 0.0 0.0 9.692728847174502 0.0 18 2.793374116595436E-4 0.0 0.0 10.60616218330121 0.0 19 5.586748233190872E-4 0.0 0.0 11.115952959579877 0.0 20 5.586748233190872E-4 0.0 0.0 11.600603368809185 0.0 21 5.586748233190872E-4 0.0 0.0 12.222967121986647 0.0 22 5.586748233190872E-4 0.0 0.0 12.841140813989217 0.0 23 5.586748233190872E-4 0.0 0.0 13.496745719154166 0.0 24 5.586748233190872E-4 0.0 0.0 13.969663957093774 0.0 25 5.586748233190872E-4 0.0 0.0 14.392860135757982 0.0 26 5.586748233190872E-4 0.0 0.0 14.786446548786278 0.0 27 5.586748233190872E-4 0.0 0.0 15.182546998519511 0.0 28 5.586748233190872E-4 0.0 0.0 15.63647029246627 0.0 29 5.586748233190872E-4 0.0 0.0 16.10156708287941 0.0 30 5.586748233190872E-4 0.0 0.0 16.61610659515629 0.0 31 5.586748233190872E-4 0.0 0.0 17.082041397804407 0.0 32 5.586748233190872E-4 0.0 0.0 17.52032179669823 0.0 33 5.586748233190872E-4 0.0 0.0 17.976479789938267 0.0 34 5.586748233190872E-4 0.0 0.0 18.42649236012179 0.0 35 5.586748233190872E-4 0.0 0.0 18.945221933573563 0.0 36 5.586748233190872E-4 0.0 0.0 19.41897818374815 0.0 37 5.586748233190872E-4 0.0 0.0 19.860889968993547 0.0 38 5.586748233190872E-4 0.0 0.0 20.301963742003966 0.0 39 5.586748233190872E-4 0.0 0.0 20.774602642531914 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AACGGGC 35 1.2087912E-7 45.000004 4 CGTCGAC 35 1.2087912E-7 45.000004 32 TCGAGCG 30 2.1612977E-6 45.000004 1 GGCGATC 30 2.1612977E-6 45.000004 8 GTAAACG 35 1.2087912E-7 45.000004 1 AACCGCG 25 3.8854065E-5 45.0 16 GGTAAGC 20 7.026771E-4 45.0 9 CGTGATG 20 7.026771E-4 45.0 17 TTTCGAG 40 6.7921064E-9 45.0 1 CGCGCAA 25 3.8854065E-5 45.0 19 CGATTTA 20 7.026771E-4 45.0 12 GGATATC 25 3.8854065E-5 45.0 8 ACGGGTC 20 7.026771E-4 45.0 5 CCCGTGC 20 7.026771E-4 45.0 15 GGACGGC 25 3.8854065E-5 45.0 8 GCGATAC 40 6.7921064E-9 45.0 9 ATGCGCG 25 3.8854065E-5 45.0 1 GCGTGAT 20 7.026771E-4 45.0 16 TCGAAAA 25 3.8854065E-5 45.0 28 TACGTGT 20 7.026771E-4 45.0 12 >>END_MODULE