##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936706.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	277000
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.1263321299639	31.0	31.0	33.0	28.0	34.0
2	31.165447653429602	31.0	31.0	34.0	28.0	34.0
3	30.900462093862817	31.0	31.0	34.0	27.0	34.0
4	34.88461010830325	35.0	35.0	37.0	32.0	37.0
5	35.13174729241877	35.0	35.0	37.0	33.0	37.0
6	35.12767148014441	36.0	35.0	37.0	32.0	37.0
7	30.934931407942237	35.0	35.0	37.0	0.0	37.0
8	32.959942238267146	35.0	35.0	37.0	17.0	37.0
9	36.180072202166066	39.0	35.0	39.0	32.0	39.0
10	36.082581227436826	37.0	35.0	39.0	32.0	39.0
11	36.348158844765344	37.0	35.0	39.0	32.0	39.0
12	36.32797472924188	38.0	35.0	39.0	32.0	39.0
13	36.07265703971119	38.0	35.0	39.0	31.0	39.0
14	37.15442960288809	39.0	36.0	40.0	32.0	41.0
15	37.28719855595668	39.0	36.0	41.0	32.0	41.0
16	37.395628158844765	39.0	36.0	40.0	32.0	41.0
17	37.18897833935018	39.0	35.0	40.0	32.0	41.0
18	37.12761010830325	39.0	36.0	40.0	32.0	41.0
19	36.88729963898917	38.0	35.0	40.0	31.0	41.0
20	36.7750036101083	38.0	35.0	40.0	31.0	41.0
21	36.92009025270758	39.0	35.0	40.0	32.0	41.0
22	36.93731046931408	39.0	35.0	40.0	32.0	41.0
23	37.01565703971119	39.0	35.0	40.0	32.0	41.0
24	36.92203249097473	39.0	35.0	40.0	32.0	41.0
25	36.69829241877256	38.0	35.0	40.0	31.0	41.0
26	36.57779061371841	38.0	35.0	40.0	31.0	41.0
27	36.660411552346574	38.0	35.0	40.0	31.0	41.0
28	36.611173285198554	38.0	35.0	40.0	31.0	41.0
29	36.5775559566787	38.0	35.0	40.0	31.0	41.0
30	36.3672202166065	38.0	35.0	40.0	30.0	41.0
31	36.28928519855596	38.0	35.0	40.0	30.0	41.0
32	36.09530685920578	38.0	35.0	40.0	30.0	41.0
33	35.84689891696751	38.0	35.0	40.0	29.0	41.0
34	35.65868231046932	38.0	35.0	40.0	28.0	41.0
35	35.636440433213	38.0	35.0	40.0	28.0	41.0
36	35.46790974729242	38.0	35.0	40.0	27.0	41.0
37	35.45725631768953	38.0	35.0	40.0	27.0	41.0
38	35.3142202166065	38.0	35.0	40.0	26.0	41.0
39	35.30087364620939	38.0	35.0	40.0	26.0	41.0
40	35.24410469314079	38.0	35.0	40.0	26.0	41.0
41	35.019602888086645	38.0	34.0	40.0	25.0	41.0
42	35.04095667870036	38.0	34.0	40.0	25.0	41.0
43	34.994465703971116	38.0	34.0	40.0	24.0	41.0
44	34.835451263537905	38.0	34.0	40.0	24.0	41.0
45	34.77336101083033	38.0	34.0	40.0	24.0	41.0
46	34.64528158844765	38.0	34.0	40.0	23.0	41.0
47	34.570750902527074	37.0	34.0	40.0	23.0	41.0
48	34.472859205776174	37.0	34.0	40.0	23.0	41.0
49	34.423830324909744	37.0	34.0	40.0	23.0	41.0
50	34.250249097472924	37.0	33.0	40.0	23.0	41.0
51	32.59181949458484	35.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	8.0
9	17.0
10	26.0
11	12.0
12	16.0
13	10.0
14	5.0
15	27.0
16	46.0
17	91.0
18	178.0
19	340.0
20	586.0
21	844.0
22	1221.0
23	1758.0
24	2348.0
25	3383.0
26	4335.0
27	4847.0
28	5074.0
29	5546.0
30	6587.0
31	8385.0
32	10996.0
33	15161.0
34	23700.0
35	26383.0
36	26669.0
37	38703.0
38	50243.0
39	39451.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.39350180505415	18.851985559566785	21.342238267148016	15.412274368231046
2	32.33285198555957	28.603249097472922	21.998916967509025	17.06498194945848
3	28.737906137184115	27.47256317689531	28.86462093862816	14.92490974729242
4	27.138628158844764	23.230324909747292	32.893140794223825	16.737906137184115
5	27.27942238267148	27.269314079422386	26.71516245487365	18.73610108303249
6	23.47436823104693	36.911552346570396	26.451985559566786	13.162093862815885
7	62.819133574007225	17.908664259927797	15.587003610108303	3.6851985559566787
8	72.88375451263538	9.968231046931407	12.727797833935018	4.420216606498196
9	67.91191335740072	7.085559566787003	14.070758122743682	10.931768953068593
10	41.80469314079423	20.641516245487367	22.36028880866426	15.193501805054153
11	33.451985559566786	22.447292418772562	26.65956678700361	17.441155234657042
12	29.225270758122747	19.909386281588446	30.812274368231048	20.053068592057762
13	24.10072202166065	25.02707581227437	33.61516245487365	17.257039711191336
14	19.57039711191336	29.447292418772562	30.623826714801446	20.358483754512633
15	17.57725631768953	24.270036101083033	40.09061371841155	18.062093862815885
16	19.982671480144404	23.328158844765344	33.73212996389891	22.957039711191335
17	20.418772563176894	22.42851985559567	33.13898916967509	24.013718411552347
18	21.309025270758124	24.334296028880868	33.61660649819495	20.740072202166065
19	21.78158844765343	27.157039711191334	29.837545126353792	21.223826714801444
20	24.445126353790613	25.06823104693141	32.37184115523466	18.114801444043323
21	22.52851985559567	28.84801444043321	30.083393501805055	18.540072202166066
22	20.72129963898917	22.83068592057762	30.76101083032491	25.687003610108306
23	19.61227436823105	27.370036101083034	31.26028880866426	21.75740072202166
24	22.750902527075812	23.03682310469314	31.072924187725633	23.139350180505417
25	18.863537906137186	28.724548736462097	30.776534296028878	21.63537906137184
26	17.84873646209386	27.870758122743684	31.257039711191336	23.02346570397112
27	21.24620938628159	28.192418772563176	28.92129963898917	21.640072202166067
28	17.900361010830327	25.136462093862814	35.74368231046932	21.219494584837545
29	21.4129963898917	24.8101083032491	32.44187725631769	21.335018050541514
30	21.897472924187724	26.62418772563177	31.269314079422383	20.209025270758122
31	24.39025270758123	24.145848375451266	28.50505415162455	22.95884476534296
32	24.41480144404332	26.042238267148015	29.204693140794223	20.33826714801444
33	22.881227436823103	23.686281588447653	27.87328519855596	25.559205776173282
34	22.611913357400724	27.169314079422385	29.49350180505415	20.725270758122743
35	22.054512635379062	24.71768953068592	28.388447653429605	24.839350180505416
36	23.488447653429603	26.131407942238265	30.345126353790615	20.035018050541517
37	23.067148014440434	25.130324909747294	32.70649819494585	19.096028880866424
38	20.97436823104693	25.917689530685923	28.018411552346574	25.08953068592058
39	23.198555956678703	25.206859205776173	30.616606498194948	20.97797833935018
40	22.493862815884476	24.34584837545126	28.037906137184116	25.122382671480143
41	22.037906137184116	24.481227436823104	29.114079422382673	24.366787003610106
42	20.561371841155236	24.746931407942238	30.90072202166065	23.790974729241874
43	23.015884476534296	24.089530685920575	29.252707581227437	23.641877256317688
44	21.66859205776173	25.00361010830325	30.079783393501806	23.248014440433213
45	20.53754512635379	24.735018050541516	29.101805054151626	25.625631768953067
46	24.737906137184115	26.458483754512635	27.336462093862817	21.467148014440433
47	19.63249097472924	24.457761732851985	33.20469314079422	22.705054151624548
48	22.575451263537907	24.225270758122743	29.419133574007223	23.78014440433213
49	19.858483754512633	22.294584837545127	33.90108303249097	23.945848375451266
50	20.36101083032491	23.470758122743682	31.640072202166063	24.528158844765343
51	20.57797833935018	24.414440433213	28.273646209386282	26.73393501805054
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	122.0
1	314.0
2	506.0
3	2420.0
4	4334.0
5	3046.0
6	1758.0
7	1689.0
8	1620.0
9	1546.5
10	1473.0
11	1485.5
12	1498.0
13	1396.0
14	1294.0
15	1232.5
16	1171.0
17	1126.0
18	1081.0
19	1100.0
20	1119.0
21	1067.5
22	1016.0
23	1038.5
24	1061.0
25	1222.0
26	1746.5
27	2110.0
28	2673.0
29	3236.0
30	3361.0
31	3486.0
32	4188.5
33	4891.0
34	5659.5
35	6428.0
36	7200.5
37	7973.0
38	8547.5
39	9122.0
40	10935.0
41	12748.0
42	14769.0
43	16790.0
44	18430.5
45	20071.0
46	28998.0
47	37925.0
48	31267.0
49	24609.0
50	23899.5
51	23190.0
52	20392.5
53	17595.0
54	15524.0
55	13453.0
56	12848.0
57	12243.0
58	11755.5
59	11268.0
60	10171.0
61	9074.0
62	7962.0
63	6850.0
64	6119.0
65	5388.0
66	4401.0
67	3414.0
68	2837.0
69	2260.0
70	1870.0
71	1480.0
72	1175.5
73	871.0
74	652.5
75	367.0
76	300.0
77	236.0
78	172.0
79	125.5
80	79.0
81	66.5
82	54.0
83	37.5
84	21.0
85	17.0
86	13.0
87	10.0
88	7.0
89	6.0
90	5.0
91	3.5
92	2.0
93	1.5
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	277000.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.95018050541516
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.12630379524936	25.08375451263538
2	8.502059777368745	5.602888086642599
3	3.3175563151897625	3.2794223826714797
4	2.0093785607853447	2.6483754512635382
5	1.3684371987027786	2.2545126353790614
6	0.9268998159347883	1.832490974729242
7	0.7143483215005697	1.647653429602888
8	0.5456218774651591	1.4382671480144404
9	0.4503023928477518	1.3353790613718413
>10	5.830922955561399	43.36425992779783
>50	0.16872644403541065	3.3328519855595666
>100	0.029581909019195374	1.756678700361011
>500	0.004382505039880796	0.9093862815884476
>1k	0.0054781312998509945	5.514079422382672
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCG	4915	1.7743682310469313	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGC	3858	1.3927797833935018	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTC	3037	1.096389891696751	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2181	0.7873646209386282	No Hit
GCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCTGC	1283	0.46317689530685924	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCT	887	0.32021660649819494	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTA	588	0.21227436823104692	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCG	535	0.1931407942238267	No Hit
GAACTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCT	509	0.18375451263537906	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTC	500	0.18050541516245489	No Hit
CCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCTGC	402	0.1451263537906137	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGC	393	0.14187725631768952	No Hit
GAATCTATCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTC	383	0.13826714801444043	No Hit
CTGTCTCTTATACACATCTGACGCAGTTCGCTTCGTATGCCGTCTTCTGCT	314	0.11335740072202166	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.12707581227436823	0.0
2	0.0	0.0	0.0	0.9375451263537906	0.0
3	0.0	0.0	0.0	1.119494584837545	0.0
4	0.0	0.0	0.0	1.5379061371841156	0.0
5	0.0	0.0	0.0	3.2902527075812276	0.0
6	0.0	0.0	0.0	3.7498194945848375	0.0
7	0.0	0.0	0.0	4.429963898916967	0.0
8	0.0	0.0	0.0	5.263537906137184	0.0
9	0.0	0.0	0.0	5.49927797833935	0.0
10	0.0	0.0	0.0	8.148375451263538	0.0
11	0.0	0.0	0.0	9.049097472924188	0.0
12	0.0	0.0	0.0	11.37870036101083	0.0
13	0.0	0.0	0.0	11.767870036101083	0.0
14	0.0	0.0	0.0	11.954873646209386	0.0
15	0.0	0.0	0.0	12.409025270758123	0.0
16	0.0	0.0	0.0	12.97725631768953	0.0
17	0.0	0.0	0.0	13.588447653429602	0.0
18	0.0	0.0	0.0	14.295667870036102	0.0
19	0.0	0.0	0.0	14.766787003610109	0.0
20	0.0	0.0	0.0	15.150902527075813	0.0
21	0.0	0.0	0.0	15.575451263537905	0.0
22	0.0	0.0	0.0	16.033574007220217	0.0
23	0.0	0.0	0.0	16.456317689530685	0.0
24	0.0	0.0	0.0	16.837545126353792	0.0
25	0.0	0.0	0.0	17.144404332129962	0.0
26	0.0	0.0	0.0	17.44187725631769	0.0
27	0.0	0.0	0.0	17.73826714801444	0.0
28	0.0	0.0	0.0	18.071841155234658	0.0
29	0.0	0.0	0.0	18.40072202166065	0.0
30	0.0	0.0	0.0	18.785559566787004	0.0
31	0.0	0.0	0.0	19.135740072202164	0.0
32	0.0	0.0	0.0	19.47797833935018	0.0
33	0.0	0.0	0.0	19.82274368231047	0.0
34	0.0	0.0	0.0	20.164259927797833	0.0
35	0.0	0.0	0.0	20.49350180505415	0.0
36	0.0	0.0	0.0	20.814079422382672	0.0
37	0.0	0.0	0.0	21.13321299638989	0.0
38	0.0	0.0	0.0	21.49927797833935	0.0
39	0.0	0.0	0.0	21.84115523465704	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACAACTC	20	7.0240063E-4	45.000004	32
GTTGACG	20	7.0240063E-4	45.000004	1
TTAGGAC	20	7.0240063E-4	45.000004	5
CGGAAAG	20	7.0240063E-4	45.000004	7
GCAAGCG	20	7.0240063E-4	45.000004	30
CAACGCG	20	7.0240063E-4	45.000004	1
TCCGAAC	20	7.0240063E-4	45.000004	17
TAGGTCG	20	7.0240063E-4	45.000004	36
CGGCACA	20	7.0240063E-4	45.000004	41
GCTATCT	20	7.0240063E-4	45.000004	4
CGCGACC	20	7.0240063E-4	45.000004	10
TTATAGC	20	7.0240063E-4	45.000004	43
GCACAAT	20	7.0240063E-4	45.000004	21
ACGGCAC	20	7.0240063E-4	45.000004	40
CGGATTG	20	7.0240063E-4	45.000004	24
CGGGACC	40	6.7830115E-9	45.000004	6
TATAGCC	20	7.0240063E-4	45.000004	44
GACGAGT	20	7.0240063E-4	45.000004	21
TGCGTGC	20	7.0240063E-4	45.000004	26
ATCTGCG	20	7.0240063E-4	45.000004	15
>>END_MODULE