FastQCFastQC Report
Sat 14 Jan 2017
SRR2936703.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2936703.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences632774
Sequences flagged as poor quality0
Sequence length51
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26410.4173686023762039No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATGCG253.88881E-545.0000041
TCCGAAA207.0308807E-445.024
TACGGGT351.2107739E-745.04
TATGTCG207.0308807E-445.01
ATCGCTC351.2107739E-745.042
TACGCGG501.0804797E-940.5000042
GTCGTTT451.925946E-840.0000049
TTTACGG403.454934E-739.3752
CGTGATA356.2438394E-638.57142643
CTACCGG356.2438394E-638.5714262
TTAATCG301.13934235E-437.49999620
TACCAAT301.13934235E-437.49999613
ACAATCG301.13934235E-437.49999618
CGTACAT301.13934235E-437.49999635
ATAACGC301.13934235E-437.49999611
TAGTGCG301.13934235E-437.4999961
CGTTTTT13350.037.415731
AACGGGA1350.036.6666644
ACGGGTA800.036.56255
TCCGAAT250.00210639436.00000424
Produced by FastQC (version 0.11.5)