##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936703.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	632774
Sequences flagged as poor quality	0
Sequence length	51
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.183255000995615	31.0	31.0	33.0	28.0	34.0
2	31.235581740084136	31.0	31.0	34.0	28.0	34.0
3	31.02922844491082	31.0	31.0	34.0	28.0	34.0
4	34.99341945149453	35.0	35.0	37.0	32.0	37.0
5	35.2067294168218	35.0	35.0	37.0	33.0	37.0
6	35.16125504524522	36.0	35.0	37.0	32.0	37.0
7	30.84599872940418	35.0	35.0	37.0	0.0	37.0
8	32.98792143798576	36.0	35.0	37.0	17.0	37.0
9	36.42468875143416	39.0	35.0	39.0	32.0	39.0
10	36.13950162301232	37.0	35.0	39.0	32.0	39.0
11	36.321400689661715	38.0	35.0	39.0	32.0	39.0
12	36.3408926409745	38.0	35.0	39.0	32.0	39.0
13	36.28884878329388	38.0	35.0	39.0	32.0	39.0
14	37.16135934788724	39.0	36.0	40.0	31.0	41.0
15	37.4165610470721	39.0	36.0	41.0	32.0	41.0
16	37.37467721492982	39.0	36.0	41.0	32.0	41.0
17	37.32027864608849	39.0	36.0	40.0	32.0	41.0
18	37.23549798190191	39.0	36.0	40.0	32.0	41.0
19	37.05945250594999	39.0	35.0	40.0	32.0	41.0
20	37.048331631830635	39.0	35.0	40.0	31.0	41.0
21	37.02413657956869	39.0	35.0	40.0	31.0	41.0
22	37.153070448532965	39.0	35.0	40.0	32.0	41.0
23	37.200736755934976	39.0	35.0	40.0	32.0	41.0
24	37.12961499682351	39.0	35.0	40.0	32.0	41.0
25	36.77168783799587	39.0	35.0	40.0	31.0	41.0
26	36.75736202814907	39.0	35.0	40.0	31.0	41.0
27	36.78028490424701	39.0	35.0	40.0	31.0	41.0
28	36.75527913599484	39.0	35.0	40.0	31.0	41.0
29	36.70622370704233	39.0	35.0	40.0	31.0	41.0
30	36.45930774652562	38.0	35.0	40.0	30.0	41.0
31	36.43480136668068	38.0	35.0	40.0	30.0	41.0
32	36.31018657530176	38.0	35.0	40.0	30.0	41.0
33	36.19185680827594	38.0	35.0	40.0	30.0	41.0
34	36.056772244118754	38.0	35.0	40.0	30.0	41.0
35	35.988386058845656	38.0	35.0	40.0	29.0	41.0
36	35.93495624030064	38.0	35.0	40.0	29.0	41.0
37	35.866645911494466	38.0	35.0	40.0	29.0	41.0
38	35.70695698622257	38.0	35.0	40.0	28.0	41.0
39	35.61673520087741	38.0	34.0	40.0	28.0	41.0
40	35.50171309187799	38.0	34.0	40.0	27.0	41.0
41	35.30410225451741	38.0	34.0	40.0	27.0	41.0
42	35.305791641249485	38.0	34.0	40.0	27.0	41.0
43	35.25666035583004	38.0	34.0	40.0	27.0	41.0
44	35.0453969347666	38.0	34.0	40.0	26.0	41.0
45	34.97779143896557	37.0	34.0	40.0	26.0	41.0
46	34.90469267068495	37.0	34.0	40.0	26.0	41.0
47	34.82600264865497	37.0	34.0	40.0	26.0	41.0
48	34.73031445666225	37.0	34.0	40.0	26.0	41.0
49	34.59295735918353	37.0	33.0	40.0	25.0	41.0
50	34.41349202084788	36.0	33.0	40.0	24.0	41.0
51	32.77057053545184	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	5.0
8	34.0
9	56.0
10	83.0
11	54.0
12	35.0
13	31.0
14	46.0
15	83.0
16	156.0
17	242.0
18	396.0
19	688.0
20	1128.0
21	1690.0
22	2530.0
23	3497.0
24	4781.0
25	6478.0
26	7962.0
27	9121.0
28	10624.0
29	12348.0
30	15236.0
31	19291.0
32	25378.0
33	34679.0
34	51744.0
35	54094.0
36	65338.0
37	93688.0
38	118253.0
39	93003.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.408581894957756	20.890396887356307	25.02378416306612	13.677237054619818
2	32.83257529544514	23.00884676045476	27.191540739663765	16.967037204436338
3	33.28534358238486	22.434866160746175	27.65252681052003	16.627263446348934
4	28.74122514515451	25.91256910050034	25.90640576256294	19.439799991782216
5	26.201455812027675	29.380473913277093	25.4648579113554	18.953212363339834
6	27.33614212973352	34.50031132758299	23.963690037833413	14.199856504850072
7	71.43103224848049	17.94337314744285	7.120551729369412	3.5050428747072413
8	84.75427245746506	4.290789444572628	7.362660286294949	3.592277811667357
9	79.08747830979149	6.3646736433545	9.454718430276845	5.0931296165771665
10	45.96570023420684	22.57630686469419	16.4294044951278	15.028588405971169
11	31.863034827600373	25.40164418892053	23.110778887880983	19.624542095598112
12	28.857064291516405	21.189397794473226	28.567071339846457	21.38646657416392
13	26.611238767711693	21.36924083480042	30.266888336120008	21.752632061367883
14	21.332102772869934	24.721306501215285	32.003527325711865	21.943063400202917
15	20.21732877773107	24.176562248132825	33.25926792188048	22.346841052255623
16	26.28758450884518	23.497014731958014	28.89104166732514	21.324359091871663
17	26.930784134619945	23.540790234744158	27.227730595757727	22.30069503487817
18	27.37075164276661	23.52419663260501	28.222872621188603	20.882179103439775
19	27.234526070919472	24.9920192675426	26.207777184271166	21.565677477266764
20	27.05958209408098	26.524161865057668	25.965826661651707	20.450429379209638
21	27.521674405079853	23.823671642640186	28.883297986326873	19.771355965953088
22	26.44751522660539	22.396305790060904	28.67943373147443	22.476745251859274
23	25.669512337738276	22.396937927285254	29.367357065871857	22.56619266910461
24	24.527872510564595	25.27790332725428	27.969859697143058	22.22436446503807
25	26.280789033683433	24.436370647340127	26.505987919857642	22.7768523991188
26	22.539168802763704	24.780253297385794	27.079810485260143	25.600767414590358
27	22.607123554381182	24.74833036755619	28.884246192163392	23.76029988589923
28	22.27525151159814	25.22448773179682	27.78653990208194	24.7137208545231
29	23.444547342337074	26.209673595944206	27.439970668832792	22.905808392885927
30	25.285330939640378	24.37315692490526	28.311845935515684	22.02966619993868
31	26.469165926539333	25.06266060236356	26.75599819208754	21.712175279009568
32	27.225518115472507	25.68547380265308	25.310616428614324	21.77839165326009
33	26.736876041050987	23.832047460862803	25.727036825154002	23.7040396729322
34	24.361620420560897	24.755125842717938	28.548897394646428	22.334356342074738
35	24.518074383587187	26.826007389684154	26.444670609095823	22.211247617632836
36	26.445460780626256	26.227057369613792	25.5818032978599	21.745678551900046
37	25.257991004687298	26.161631166893713	25.752164279821866	22.82821354859713
38	25.131563559817565	26.689307714918755	25.142151858325406	23.036976866938275
39	23.787323752240138	24.687803228324807	26.075976572994463	25.448896446440592
40	24.54430807839766	23.12310556375578	28.289088995439133	24.043497362407432
41	22.40831639732353	24.970368567608656	27.82146548372721	24.799849551340607
42	23.161823968747136	24.980482763198236	28.410933445432335	23.446759822622294
43	22.53822059692718	23.664056993492146	28.587773833943874	25.209948575636798
44	23.978545262605603	23.533046553745887	27.394772857291862	25.093635326356644
45	24.249416063239007	23.493379942918008	26.63684032529782	25.620363668545167
46	23.916437780313352	23.609535157892076	27.25870531975081	25.215321742043763
47	22.68835318770999	24.776934576957967	28.580978358782122	23.953733876549922
48	22.60364679964727	25.77413104836798	28.032283248047484	23.589938903937266
49	24.514439594547184	24.22128595675549	28.2734435991365	22.990830849560822
50	23.744812523902688	23.804865560215813	28.315796793167863	24.134525122713637
51	23.27086763994728	24.657144572943896	27.11758068441497	24.954407102693853
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	254.0
1	697.0
2	1140.0
3	2940.0
4	4740.0
5	3250.5
6	1761.0
7	1675.5
8	1590.0
9	1569.5
10	1549.0
11	1552.5
12	1556.0
13	1572.0
14	1588.0
15	1627.0
16	1666.0
17	1598.0
18	1530.0
19	1463.5
20	1397.0
21	1535.0
22	1673.0
23	2101.0
24	2529.0
25	3077.5
26	4178.0
27	4730.0
28	5525.5
29	6321.0
30	7285.5
31	8250.0
32	9966.5
33	11683.0
34	13230.5
35	14778.0
36	15882.5
37	16987.0
38	18418.0
39	19849.0
40	21741.0
41	23633.0
42	26400.0
43	29167.0
44	32271.0
45	35375.0
46	38654.5
47	41934.0
48	45354.0
49	48774.0
50	48264.5
51	47755.0
52	44980.5
53	42206.0
54	40632.5
55	39059.0
56	40167.5
57	41276.0
58	40989.0
59	40702.0
60	37247.5
61	33793.0
62	30383.5
63	26974.0
64	24360.0
65	21746.0
66	18724.0
67	15702.0
68	13940.5
69	12179.0
70	10523.0
71	8867.0
72	7394.0
73	5921.0
74	4996.5
75	3141.5
76	2211.0
77	1661.5
78	1112.0
79	798.5
80	485.0
81	380.0
82	275.0
83	228.0
84	181.0
85	131.0
86	81.0
87	59.0
88	37.0
89	30.0
90	23.0
91	21.5
92	20.0
93	14.5
94	9.0
95	7.5
96	6.0
97	3.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	632774.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.7010373243303
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.19907094094678	24.331835845628188
2	8.73399174908754	5.8868916385278425
3	4.06665899315652	4.111518795410742
4	2.50359783713428	3.374953766175
5	1.7159712563725762	2.8915005679245076
6	1.1863952496466155	2.3989650353849252
7	0.8890840829468404	2.097413910470161
8	0.6935569448883498	1.869887078898462
9	0.594387856875408	1.8028338614718138
>10	7.3452114604311225	48.15240275352204
>50	0.04789408862713316	1.0877021874912236
>100	0.02371454873517632	1.5802317904844692
>500	0.0	0.0
>1k	4.64991151670124E-4	0.41386276861061916
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2641	0.4173686023762039	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.06542620272008648	0.0
2	0.0	0.0	0.0	0.2621789137986074	0.0
3	0.0	0.0	0.0	0.36537531567352644	0.0
4	0.0	0.0	0.0	0.5126632889467646	0.0
5	0.0	0.0	0.0	0.9164409409994722	0.0
6	0.0	0.0	0.0	1.3285944112747996	0.0
7	0.0	0.0	0.0	1.6555673905691448	0.0
8	1.580343060871654E-4	0.0	0.0	2.2064749815890035	0.0
9	1.580343060871654E-4	0.0	0.0	2.5032634084207	0.0
10	1.580343060871654E-4	0.0	0.0	2.9825814587830726	0.0
11	1.580343060871654E-4	0.0	0.0	3.616299026192606	0.0
12	3.160686121743308E-4	0.0	0.0	4.109998198408911	0.0
13	3.160686121743308E-4	0.0	0.0	4.3611147107814165	0.0
14	3.160686121743308E-4	0.0	0.0	4.49971079721986	0.0
15	3.160686121743308E-4	0.0	0.0	4.631037305578295	0.0
16	3.160686121743308E-4	0.0	0.0	4.899221523008214	0.0
17	3.160686121743308E-4	0.0	0.0	5.2042277337564435	0.0
18	3.160686121743308E-4	0.0	0.0	5.58635468587521	0.0
19	3.160686121743308E-4	0.0	0.0	5.796856381583314	0.0
20	3.160686121743308E-4	0.0	0.0	6.042283658936682	0.0
21	3.160686121743308E-4	0.0	0.0	6.323426689465749	0.0
22	3.160686121743308E-4	0.0	0.0	6.631909654947896	0.0
23	3.160686121743308E-4	0.0	0.0	6.924273121209152	0.0
24	3.160686121743308E-4	0.0	0.0	7.158479962830331	0.0
25	3.160686121743308E-4	0.0	0.0	7.368665589926261	0.0
26	3.160686121743308E-4	0.0	0.0	7.574900359370012	0.0
27	3.160686121743308E-4	0.0	0.0	7.8193794308868565	0.0
28	3.160686121743308E-4	0.0	0.0	8.038731047735842	0.0
29	3.160686121743308E-4	0.0	0.0	8.285422599537908	0.0
30	3.160686121743308E-4	0.0	0.0	8.615714299260084	0.0
31	3.160686121743308E-4	0.0	0.0	8.870307566366506	0.0
32	3.160686121743308E-4	0.0	0.0	9.117947324005096	0.0
33	3.160686121743308E-4	0.0	0.0	9.388502056026322	0.0
34	4.741029182614962E-4	0.0	0.0	9.687502963143238	0.0
35	4.741029182614962E-4	0.0	0.0	9.989190453463637	0.0
36	4.741029182614962E-4	0.0	0.0	10.248050646834415	0.0
37	4.741029182614962E-4	0.0	0.0	10.54215249046263	0.0
38	4.741029182614962E-4	0.0	0.0	10.898203782077013	0.0
39	4.741029182614962E-4	0.0	0.0	11.550569397604832	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATGCG	25	3.88881E-5	45.000004	1
TCCGAAA	20	7.0308807E-4	45.0	24
TACGGGT	35	1.2107739E-7	45.0	4
TATGTCG	20	7.0308807E-4	45.0	1
ATCGCTC	35	1.2107739E-7	45.0	42
TACGCGG	50	1.0804797E-9	40.500004	2
GTCGTTT	45	1.925946E-8	40.000004	9
TTTACGG	40	3.454934E-7	39.375	2
CGTGATA	35	6.2438394E-6	38.571426	43
CTACCGG	35	6.2438394E-6	38.571426	2
TTAATCG	30	1.13934235E-4	37.499996	20
TACCAAT	30	1.13934235E-4	37.499996	13
ACAATCG	30	1.13934235E-4	37.499996	18
CGTACAT	30	1.13934235E-4	37.499996	35
ATAACGC	30	1.13934235E-4	37.499996	11
TAGTGCG	30	1.13934235E-4	37.499996	1
CGTTTTT	1335	0.0	37.41573	1
AACGGGA	135	0.0	36.666664	4
ACGGGTA	80	0.0	36.5625	5
TCCGAAT	25	0.002106394	36.000004	24
>>END_MODULE