FastQCFastQC Report
Sat 14 Jan 2017
SRR2936701.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2936701.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences793723
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT63060.7944837178713481No Hit
GAATCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTC10780.13581564349275502No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCG9250.11653939724563858No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT8180.10305862372641336No Hit
GCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCTGC8140.10255466957616195No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGC8020.10104280712540774No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCAGTA253.8897102E-545.00000437
CGCATTG207.031966E-445.031
CACTCGA207.031966E-445.041
CGTTTTT31150.042.7608341
CGAATAT1350.041.66666414
CCATACG556.002665E-1140.909092
AACCCGC501.0804797E-940.50000421
TCACGAC800.039.37525
CGGTAGT1150.039.13043212
TACGAAT1450.038.79310212
ATAGGCG356.2458457E-638.5714265
TATAGCG356.2458457E-638.5714261
CGGATCT301.13960356E-437.50000412
ATAACGG301.13960356E-437.5000042
CCCGCAT552.746674E-936.8181823
CACGACG800.036.562526
CGGCGTA250.002106716236.00000415
CAACGAT250.002106716236.00000437
CGACGGT751.8189894E-1236.00000428
GCGCATC250.002106716236.00000425
Produced by FastQC (version 0.11.5)