##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936701.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	793723
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.189694641581507	31.0	31.0	33.0	28.0	34.0
2	31.24881728260363	31.0	31.0	34.0	28.0	34.0
3	30.999623294272688	31.0	31.0	34.0	28.0	34.0
4	34.97135902575584	35.0	35.0	37.0	32.0	37.0
5	35.212804719026664	35.0	35.0	37.0	33.0	37.0
6	35.19611627734109	37.0	35.0	37.0	32.0	37.0
7	31.049107812171247	35.0	35.0	37.0	0.0	37.0
8	33.10671355120111	36.0	35.0	37.0	17.0	37.0
9	36.4665015376901	39.0	35.0	39.0	32.0	39.0
10	36.151368928454886	37.0	35.0	39.0	32.0	39.0
11	36.464190907911195	38.0	35.0	39.0	32.0	39.0
12	36.5693850373493	38.0	35.0	39.0	32.0	39.0
13	36.59803105113497	39.0	35.0	39.0	32.0	39.0
14	37.704640031849905	39.0	37.0	41.0	33.0	41.0
15	37.872629368180085	40.0	37.0	41.0	33.0	41.0
16	37.74587608019422	39.0	37.0	41.0	33.0	41.0
17	37.65599585749688	39.0	36.0	41.0	33.0	41.0
18	37.55465067788133	39.0	36.0	40.0	33.0	41.0
19	37.33017690050559	39.0	36.0	40.0	32.0	41.0
20	37.30821583852301	39.0	35.0	40.0	32.0	41.0
21	37.30978691558642	39.0	35.0	40.0	32.0	41.0
22	37.3875734985631	39.0	35.0	40.0	32.0	41.0
23	37.41617037681912	39.0	35.0	41.0	33.0	41.0
24	37.359702314283446	39.0	35.0	41.0	32.0	41.0
25	37.03894053718992	39.0	35.0	40.0	32.0	41.0
26	37.025891904354545	39.0	35.0	40.0	32.0	41.0
27	37.047379249435885	39.0	35.0	40.0	32.0	41.0
28	37.03821736298431	39.0	35.0	40.0	32.0	41.0
29	37.0026621377987	39.0	35.0	40.0	31.0	41.0
30	36.76169394108524	39.0	35.0	40.0	31.0	41.0
31	36.72832083737022	39.0	35.0	40.0	31.0	41.0
32	36.548732996272	39.0	35.0	40.0	30.0	41.0
33	36.39589882112526	39.0	35.0	40.0	30.0	41.0
34	36.27937202273337	39.0	35.0	40.0	30.0	41.0
35	36.18363585281011	39.0	35.0	40.0	30.0	41.0
36	36.10585304948956	39.0	35.0	40.0	29.0	41.0
37	36.040894619407524	39.0	35.0	40.0	29.0	41.0
38	35.928517883442964	39.0	35.0	40.0	29.0	41.0
39	35.88698576203537	39.0	35.0	40.0	29.0	41.0
40	35.80615025644967	39.0	35.0	40.0	28.0	41.0
41	35.638589281147205	38.0	35.0	40.0	27.0	41.0
42	35.630005682083045	38.0	35.0	40.0	27.0	41.0
43	35.561564928822776	38.0	35.0	40.0	27.0	41.0
44	35.36495099675832	38.0	34.0	40.0	26.0	41.0
45	35.29599873003554	38.0	34.0	40.0	26.0	41.0
46	35.19357634842382	38.0	34.0	40.0	26.0	41.0
47	35.15757764358599	38.0	34.0	40.0	26.0	41.0
48	35.08211806889809	38.0	34.0	40.0	26.0	41.0
49	34.954371991236236	38.0	34.0	40.0	26.0	41.0
50	34.825001921325196	37.0	34.0	40.0	24.0	41.0
51	33.19629392117905	36.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	30.0
9	74.0
10	73.0
11	65.0
12	48.0
13	46.0
14	44.0
15	67.0
16	120.0
17	227.0
18	414.0
19	716.0
20	1205.0
21	1745.0
22	2661.0
23	3950.0
24	5758.0
25	8280.0
26	10447.0
27	11769.0
28	12789.0
29	14442.0
30	17193.0
31	21158.0
32	27396.0
33	37568.0
34	57507.0
35	65465.0
36	78837.0
37	119275.0
38	161175.0
39	133168.0
40	7.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.053238976317935	20.53461976029421	23.020121629334163	15.392019634053694
2	34.60149195626182	23.397457299334906	24.720840897895112	17.280209846508164
3	32.20279618960267	22.728710141951286	28.928858052494384	16.13963561595166
4	28.92205467146599	25.202873042610584	27.21629586140253	18.658776424520898
5	25.05193877461029	29.73379881898345	26.715743401665314	18.49851900474095
6	25.7671757023546	34.04185087240763	26.309934322175373	13.881039103062404
7	68.81708102196862	17.746241446953157	9.66382478522104	3.7728527458571817
8	80.80917398135118	5.379080611245988	10.0417903979096	3.769955009493237
9	74.98648772934638	7.681899100819807	11.848466026560905	5.483147143272905
10	38.63262120412285	27.473816432180996	19.14080856923637	14.75275379445978
11	29.983634088970586	26.084162862862737	25.543924013793223	18.388279034373454
12	27.85593966660913	22.92764604276303	29.86596079488688	19.350453495740958
13	24.85350682794879	22.792082376345398	31.45152654011538	20.902884255590426
14	21.040085772996374	25.918613924505145	31.353885423504167	21.68741487899431
15	20.75724150616777	25.808499942675216	33.17542769958789	20.258830851569122
16	24.117607780044175	24.66162628524057	31.360562815995003	19.86020311872026
17	23.767989588307255	24.944722529144297	29.7024276731303	21.584860209418146
18	24.362529497066358	25.170493988456933	30.063132856172746	20.403843658303966
19	24.516991444118414	26.615834491377978	28.3261288887937	20.54104517570991
20	25.402060920497455	25.821350773506623	28.9284800868817	19.848108219114224
21	24.863459922416258	26.276295382646087	29.60264475138052	19.257599943557135
22	24.34413517058218	24.11748179150661	29.23601810707262	22.302364930838593
23	23.303469850313018	25.794263237930615	29.66412715771119	21.238139754045175
24	23.27134277323449	24.727266313310814	29.959066324145827	22.042324589308866
25	22.983584953440936	25.78443613200071	28.74189106275111	22.490087851807242
26	21.87035023553557	27.029454860196818	28.951410000718138	22.148784903549476
27	22.598941948261547	25.954898623323253	29.10927363828439	22.336885790130815
28	21.241037490409123	27.056920361385522	30.097149761314718	21.60489238689064
29	22.395974414247792	26.204229939160133	29.61373174268605	21.786063903906022
30	22.8856918597546	25.93096080118631	29.899473745878602	21.28387359318049
31	23.448104691435173	25.85700552963691	28.415706739000886	22.279183039927027
32	23.986831678053928	26.52499675579516	28.07704955003194	21.411122016118973
33	23.249798733311245	25.4466608627947	28.585791264710736	22.717749139183315
34	21.65843751535485	25.859147334775486	29.97670471940463	22.505710430465037
35	22.146517109873344	25.58890192170316	28.58062573467066	23.683955233752833
36	22.16932103517222	27.446728896604988	28.510450119248148	21.873499948974644
37	22.90068449572458	26.903970276784218	28.361027713698604	21.834317513792595
38	21.663729033932494	27.167538297365706	28.166753388776687	23.00197927992511
39	22.320255303172516	25.1760374841097	28.754489916507396	23.74921729621039
40	23.225608934099178	25.094270923231406	29.222789310628517	22.4573308320409
41	20.374488329051825	26.213553090939783	29.411646128435237	24.000312451573155
42	22.092216050183755	26.177268392121682	28.74516676472775	22.985348792966818
43	22.113886078644565	25.55639687901195	28.853264929956673	23.476452112386813
44	21.999992440687745	25.978962433997754	28.673605275392045	23.347439849922456
45	22.22450401462475	25.44262922959269	28.137776025137235	24.195090730645326
46	22.084026795242167	26.87171721116813	27.777952761857726	23.26630323173198
47	21.442367173434562	25.593059543442738	30.000642541541573	22.96393074158113
48	21.416035569083924	25.281111924437116	30.05494360123116	23.2479089052478
49	21.33855261848277	25.186116567114723	30.067920420600135	23.407410393802373
50	20.258956840106688	24.815080323992124	30.547684771639478	24.378278064261714
51	20.32094320058761	24.24120253539333	30.069306294513325	25.368547969505734
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	301.0
1	836.5
2	1372.0
3	6440.5
4	11509.0
5	7820.5
6	4132.0
7	3792.5
8	3453.0
9	3432.0
10	3411.0
11	3360.5
12	3310.0
13	3150.5
14	2991.0
15	2943.0
16	2895.0
17	2886.5
18	2878.0
19	2895.5
20	2913.0
21	3008.0
22	3103.0
23	3410.0
24	3717.0
25	4303.0
26	5831.0
27	6773.0
28	8179.5
29	9586.0
30	11120.5
31	12655.0
32	14938.5
33	17222.0
34	20122.0
35	23022.0
36	24758.0
37	26494.0
38	28076.0
39	29658.0
40	32233.0
41	34808.0
42	38203.0
43	41598.0
44	45316.0
45	49034.0
46	55478.5
47	61923.0
48	63057.5
49	64192.0
50	63751.0
51	63310.0
52	57582.0
53	51854.0
54	49107.0
55	46360.0
56	44571.0
57	42782.0
58	40832.5
59	38883.0
60	37602.0
61	36321.0
62	32756.5
63	29192.0
64	25034.5
65	20877.0
66	17135.5
67	13394.0
68	10917.0
69	8440.0
70	6911.0
71	5382.0
72	4718.0
73	4054.0
74	3230.5
75	1840.5
76	1274.0
77	979.5
78	685.0
79	514.5
80	344.0
81	248.5
82	153.0
83	108.5
84	64.0
85	52.5
86	41.0
87	42.0
88	43.0
89	27.0
90	11.0
91	7.5
92	4.0
93	4.0
94	4.0
95	3.5
96	3.0
97	2.5
98	2.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	793723.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.86864985619097
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.67309495120551	26.066857967705104
2	9.146209429016013	6.561243670415354
3	4.1587468882589524	4.475059079264524
4	2.4827610973599827	3.5621315391111077
5	1.6515208383517115	2.961891134052025
6	1.2047974155721177	2.5928673988080053
7	0.8783019314620243	2.205245311333931
8	0.7089447169396211	2.0343111855442952
9	0.6194233141840785	1.9996090172307073
>10	6.399734732202488	43.46283196183822
>50	0.05648743182032278	1.3256311454277117
>100	0.016532899553482073	1.124376949588826
>500	0.002755483258912783	0.7156923051276679
>1k	3.444354073640979E-4	0.1331807880075308
>5k	3.444354073640979E-4	0.7790705465449808
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6306	0.7944837178713481	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTC	1078	0.13581564349275502	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCG	925	0.11653939724563858	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	818	0.10305862372641336	No Hit
GCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGCCGTCTTCTGC	814	0.10255466957616195	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTCTCCTTCGTATGC	802	0.10104280712540774	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.2598853756285252E-4	0.0	0.0	0.07004962688494601	0.0
2	1.2598853756285252E-4	0.0	0.0	0.33500352137962486	0.0
3	1.2598853756285252E-4	0.0	0.0	0.5166789925452583	0.0
4	1.2598853756285252E-4	0.0	0.0	0.743206383083267	0.0
5	1.2598853756285252E-4	0.0	0.0	1.3427858333448823	0.0
6	1.2598853756285252E-4	0.0	0.0	1.9201913009954354	0.0
7	1.2598853756285252E-4	0.0	0.0	2.3309139334503346	0.0
8	1.2598853756285252E-4	0.0	0.0	2.9685419220559313	0.0
9	1.2598853756285252E-4	0.0	0.0	3.261213294814438	0.0
10	1.2598853756285252E-4	0.0	0.0	3.905140710298177	0.0
11	1.2598853756285252E-4	0.0	0.0	4.546044400880408	0.0
12	1.2598853756285252E-4	0.0	0.0	5.160238521499314	0.0
13	1.2598853756285252E-4	0.0	0.0	5.419018977653413	0.0
14	1.2598853756285252E-4	0.0	0.0	5.545385480828954	0.0
15	1.2598853756285252E-4	0.0	0.0	5.7271869405321505	0.0
16	1.2598853756285252E-4	0.0	0.0	6.060930576536147	0.0
17	1.2598853756285252E-4	0.0	0.0	6.428943094757239	0.0
18	1.2598853756285252E-4	0.0	0.0	6.861713721285637	0.0
19	1.2598853756285252E-4	0.0	0.0	7.103989679019003	0.0
20	1.2598853756285252E-4	0.0	0.0	7.377384805530393	0.0
21	1.2598853756285252E-4	0.0	0.0	7.707096808332378	0.0
22	1.2598853756285252E-4	0.0	0.0	8.049533653428211	0.0
23	1.2598853756285252E-4	0.0	0.0	8.407719065719402	0.0
24	1.2598853756285252E-4	0.0	0.0	8.691319263773382	0.0
25	1.2598853756285252E-4	0.0	0.0	8.9527454792163	0.0
26	1.2598853756285252E-4	0.0	0.0	9.194517482799414	0.0
27	1.2598853756285252E-4	0.0	0.0	9.456321663855022	0.0
28	1.2598853756285252E-4	0.0	0.0	9.741811689972446	0.0
29	1.2598853756285252E-4	0.0	0.0	10.028939567078186	0.0
30	1.2598853756285252E-4	0.0	0.0	10.368982629960326	0.0
31	1.2598853756285252E-4	0.0	0.0	10.67211105133655	0.0
32	1.2598853756285252E-4	0.0	0.0	10.985570532792927	0.0
33	1.2598853756285252E-4	0.0	0.0	11.295754312272669	0.0
34	1.2598853756285252E-4	0.0	0.0	11.592835283845876	0.0
35	1.2598853756285252E-4	0.0	0.0	11.915491928544341	0.0
36	1.2598853756285252E-4	0.0	0.0	12.218620349920565	0.0
37	1.2598853756285252E-4	0.0	0.0	12.54215891438197	0.0
38	1.2598853756285252E-4	0.0	0.0	12.877666389911846	0.0
39	1.2598853756285252E-4	0.0	0.0	13.20687443856358	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCAGTA	25	3.8897102E-5	45.000004	37
CGCATTG	20	7.031966E-4	45.0	31
CACTCGA	20	7.031966E-4	45.0	41
CGTTTTT	3115	0.0	42.760834	1
CGAATAT	135	0.0	41.666664	14
CCATACG	55	6.002665E-11	40.90909	2
AACCCGC	50	1.0804797E-9	40.500004	21
TCACGAC	80	0.0	39.375	25
CGGTAGT	115	0.0	39.130432	12
TACGAAT	145	0.0	38.793102	12
ATAGGCG	35	6.2458457E-6	38.571426	5
TATAGCG	35	6.2458457E-6	38.571426	1
CGGATCT	30	1.13960356E-4	37.500004	12
ATAACGG	30	1.13960356E-4	37.500004	2
CCCGCAT	55	2.746674E-9	36.81818	23
CACGACG	80	0.0	36.5625	26
CGGCGTA	25	0.0021067162	36.000004	15
CAACGAT	25	0.0021067162	36.000004	37
CGACGGT	75	1.8189894E-12	36.000004	28
GCGCATC	25	0.0021067162	36.000004	25
>>END_MODULE