##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2936700.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 255613 Sequences flagged as poor quality 0 Sequence length 51 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.021008321173024 31.0 31.0 33.0 28.0 34.0 2 31.066620242319445 31.0 31.0 34.0 28.0 34.0 3 30.839605184399854 31.0 30.0 34.0 27.0 34.0 4 34.84231631411548 35.0 35.0 37.0 32.0 37.0 5 35.0536944521601 35.0 35.0 37.0 33.0 37.0 6 35.03213060368604 35.0 35.0 37.0 32.0 37.0 7 31.102162253093542 35.0 35.0 37.0 0.0 37.0 8 32.93948273366378 35.0 35.0 37.0 17.0 37.0 9 35.91125255757728 38.0 34.0 39.0 32.0 39.0 10 35.98525505353796 37.0 35.0 39.0 32.0 39.0 11 36.19644540770618 37.0 35.0 39.0 32.0 39.0 12 36.1740326196242 37.0 35.0 39.0 32.0 39.0 13 35.882826773286176 37.0 35.0 39.0 31.0 39.0 14 37.01034376185875 39.0 35.0 40.0 31.0 41.0 15 37.08776548923568 39.0 35.0 40.0 32.0 41.0 16 37.28184012550222 39.0 36.0 40.0 32.0 41.0 17 36.97716078603201 39.0 35.0 40.0 31.0 41.0 18 36.8975325981073 38.0 35.0 40.0 31.0 41.0 19 36.66046327847175 38.0 35.0 40.0 31.0 41.0 20 36.49328085817231 38.0 35.0 40.0 31.0 41.0 21 36.72450540465469 38.0 35.0 40.0 31.0 41.0 22 36.7580874212188 38.0 35.0 40.0 31.0 41.0 23 36.81013876446033 38.0 35.0 40.0 31.0 41.0 24 36.692363064476375 38.0 35.0 40.0 31.0 41.0 25 36.496676616604006 38.0 35.0 40.0 31.0 41.0 26 36.360142089799815 38.0 35.0 40.0 31.0 41.0 27 36.34903154377907 38.0 35.0 40.0 31.0 41.0 28 36.30080629701932 38.0 35.0 40.0 30.0 41.0 29 36.23894324623552 38.0 35.0 40.0 30.0 41.0 30 36.05510283123316 38.0 35.0 40.0 30.0 41.0 31 35.99103331990157 38.0 35.0 40.0 30.0 41.0 32 35.74697296303396 38.0 35.0 40.0 29.0 41.0 33 35.549498656171636 38.0 35.0 40.0 27.0 41.0 34 35.38861482006001 38.0 34.0 40.0 27.0 41.0 35 35.290536866278316 38.0 34.0 40.0 26.0 41.0 36 35.20926948159914 38.0 34.0 40.0 26.0 41.0 37 34.99889285756202 38.0 34.0 40.0 24.0 41.0 38 34.886116903287395 38.0 34.0 40.0 24.0 41.0 39 34.865640636430854 38.0 34.0 40.0 24.0 41.0 40 34.71747133361762 38.0 34.0 40.0 24.0 41.0 41 34.57628524370826 37.0 34.0 40.0 23.0 41.0 42 34.43861618931744 37.0 33.0 40.0 23.0 41.0 43 34.42901573863614 37.0 33.0 40.0 23.0 41.0 44 34.17272204465344 37.0 33.0 40.0 23.0 41.0 45 34.211663726023325 37.0 33.0 40.0 23.0 41.0 46 34.13814633841002 37.0 33.0 40.0 23.0 41.0 47 33.945906507102535 37.0 33.0 40.0 22.0 41.0 48 33.93438518385215 37.0 33.0 40.0 22.0 41.0 49 33.85544944897169 36.0 33.0 40.0 22.0 41.0 50 33.69828608091138 36.0 33.0 40.0 21.0 41.0 51 31.95475582227821 35.0 30.0 39.0 15.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 15.0 9 10.0 10 19.0 11 17.0 12 14.0 13 9.0 14 10.0 15 23.0 16 46.0 17 105.0 18 222.0 19 405.0 20 638.0 21 1022.0 22 1356.0 23 1890.0 24 2499.0 25 3417.0 26 4179.0 27 4729.0 28 5221.0 29 5933.0 30 6837.0 31 8725.0 32 11248.0 33 15210.0 34 23046.0 35 24441.0 36 24265.0 37 33986.0 38 43760.0 39 32315.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 48.316008966680094 17.299589613986768 19.918001040635648 14.466400378697482 2 31.671315621662433 30.9757328461385 21.78566817806606 15.567283354133005 3 28.637823584872447 29.940965443854576 27.11755661879482 14.30365435247816 4 27.625355517911842 21.57362888428992 34.39731156083611 16.40370403696213 5 29.445685469831346 26.305000136925745 24.809771021035708 19.439543372207204 6 22.91471873496262 39.35989171129794 25.20216107944432 12.523228474295125 7 62.0277528920673 16.9228482119454 17.39974101473712 3.6496578812501714 8 70.29415561806324 12.15118166916393 12.527531854796118 5.027130857976707 9 65.04207532480743 7.584903741202521 13.427329595912571 13.945691338077484 10 42.64415346637299 22.345107643194986 20.433233051527115 14.577505838904909 11 35.48371952913193 22.354105620606152 25.037067754769904 17.125107095492012 12 31.665447375524717 19.795550304561974 28.58540058604218 19.953601733871125 13 23.78048064848032 24.163872729477763 34.014701912657024 18.040944709384892 14 19.974727419966904 30.501578558211044 28.080731418198607 21.442962603623446 15 17.045298948019074 23.792608357164934 40.92593099724975 18.236161697566242 16 20.576418257287386 23.642772472448584 32.32464702499482 23.456162245269216 17 19.83349829625254 23.19326481829954 33.40753404560801 23.565702839839915 18 21.115905685548075 24.430291104130074 31.893917758486463 22.55988545183539 19 22.931149824148225 27.170762050443443 28.394878194771 21.503209930637333 20 26.606236771995164 24.706489889011905 31.415460090058016 17.271813248934915 21 23.644728554494492 30.243766944560726 27.812356961500395 18.299147539444395 22 21.14798543110092 23.493327804141416 30.614640100464374 24.744046664293286 23 22.25943124958433 28.55058232562507 29.547401736218426 19.642584688572175 24 23.43933993967443 22.853688975130375 30.08415064961485 23.62282043558035 25 19.777554349739646 29.976957353499234 27.798664387179056 22.446823909582065 26 20.953942092147113 25.2768834135979 30.6823205392527 23.08685395500229 27 22.25160692140071 28.187142281495852 27.00136534526804 22.55988545183539 28 17.990869009009714 27.359328359668716 34.03700124798035 20.61280138334122 29 24.596949294441206 25.01946301635676 28.554494489716873 21.829093199485158 30 20.177377519922697 28.662861435060034 29.7793930668628 21.380367978154478 31 27.063177537918648 26.300696756424752 24.831287923540664 21.804837782115932 32 26.07222637346301 31.260929608431496 24.637635800996037 18.029208217109456 33 24.068415925637584 27.49899261774636 24.141182177745264 24.291409278870795 34 26.864830818463847 27.454785163508898 26.312042032291 19.36834198573625 35 21.02944685911906 27.91368201147829 25.78233501425984 25.274536115142816 36 27.56197845962451 30.160437849405152 24.369261344297826 17.90832234667251 37 21.769237088880455 30.885753072026855 26.194677109536684 21.15033272955601 38 20.69613047849679 34.59839679515518 22.415135380438397 22.290337345909634 39 26.475961707737873 27.928939451436353 26.10900071592603 19.48609812489975 40 22.234784615805925 30.354089971949783 27.355024979167723 20.056100433076566 41 24.610250652353365 26.7212543962944 25.979116868077917 22.689378083274324 42 21.120600282458245 28.21804837782116 28.439868081826823 22.22148325789377 43 26.731034806523922 24.622378361037974 25.044109650135166 23.602477182302934 44 22.116246043824063 26.129343969203443 27.875342803378544 23.87906718359395 45 20.647228427349155 25.714263359062333 26.89925786247178 26.739250351116727 46 26.147731140434953 28.217265945002794 25.1137461709694 20.521256743592854 47 20.378853970650944 25.228763795268627 33.38719079233059 21.005191441749833 48 22.6361726516257 26.410628567404633 26.75137805980134 24.20182072116833 49 20.768114297786106 23.88767394459593 32.93416219049892 22.410049567119042 50 21.75632694737748 25.01007382253641 28.830693274598712 24.402905955487398 51 21.335769307507835 24.803902774897992 25.89031074319381 27.970017174400365 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 113.0 1 386.5 2 660.0 3 2479.0 4 4298.0 5 2922.5 6 1547.0 7 1431.0 8 1315.0 9 1284.5 10 1254.0 11 1145.0 12 1036.0 13 1001.0 14 966.0 15 969.0 16 972.0 17 939.5 18 907.0 19 915.0 20 923.0 21 920.0 22 917.0 23 999.0 24 1081.0 25 1282.5 26 1803.5 27 2123.0 28 2519.5 29 2916.0 30 3461.5 31 4007.0 32 4556.0 33 5105.0 34 5395.0 35 5685.0 36 5935.0 37 6185.0 38 7174.0 39 8163.0 40 9535.5 41 10908.0 42 12105.5 43 13303.0 44 14918.0 45 16533.0 46 27117.5 47 37702.0 48 29333.0 49 20964.0 50 20176.5 51 19389.0 52 17067.5 53 14746.0 54 13644.5 55 12543.0 56 11685.5 57 10828.0 58 10792.5 59 10757.0 60 10835.0 61 10913.0 62 9927.0 63 8941.0 64 7765.0 65 6589.0 66 5073.5 67 3558.0 68 3007.5 69 2457.0 70 2033.0 71 1609.0 72 1286.0 73 963.0 74 765.0 75 437.5 76 308.0 77 260.0 78 212.0 79 154.0 80 96.0 81 68.0 82 40.0 83 26.0 84 12.0 85 9.5 86 7.0 87 6.0 88 5.0 89 3.5 90 2.0 91 2.0 92 2.0 93 1.0 94 0.0 95 0.5 96 1.0 97 1.0 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 255613.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.14854878273014 #Duplication Level Percentage of deduplicated Percentage of total 1 75.53226644006988 25.03785018758827 2 8.178728225463814 5.422259431249584 3 3.3222395316999482 3.3038225755341077 4 2.0653354104706603 2.738514864267467 5 1.3843648208469055 2.2944842398469563 6 1.057451730160978 2.1031794157574146 7 0.8178728225463816 1.8977908009373545 8 0.67034886465562 1.777687363318767 9 0.5700325732899023 1.7006177307100971 >10 6.267997922862673 41.035471591820446 >50 0.0849737997450786 1.8797948461150256 >100 0.03776613322003493 2.286268695254154 >500 0.003540574989378275 0.8000375567752814 >1k 0.004720766652504366 3.4352712890189463 >5k 0.002360383326252183 4.286949411806129 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAATGATACGGCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGC 5604 2.192376757050698 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCG 5354 2.094572654755431 No Hit GAATCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTTC 3930 1.537480488081592 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 2272 0.8888436816593835 No Hit GCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTTCTGC 1520 0.5946489419552213 No Hit GAATGACTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCT 1059 0.41429817732274965 No Hit GAATGATCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTC 772 0.3020190678877835 No Hit GAACTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTTCT 693 0.2711129715624792 No Hit GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTA 580 0.22690551732501865 No Hit GAATGAAACGGCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGC 489 0.1913048240895416 No Hit GAATGAAACCTGTCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCG 377 0.14748858626126216 No Hit GAATCTATCTCTTATACACATCTGACGCGCAAAGAGTCGTATGCCGTCTTC 305 0.11932100480022534 No Hit AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG 269 0.10523721406970694 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.1197122212094064 0.0 2 0.0 0.0 0.0 1.0355498351022836 0.0 3 0.0 0.0 0.0 1.1912539659563481 0.0 4 0.0 0.0 0.0 1.7092244917120805 0.0 5 0.0 0.0 0.0 3.999796567467226 0.0 6 0.0 0.0 0.0 4.426222453474589 0.0 7 0.0 0.0 0.0 5.189876884196031 0.0 8 0.0 0.0 0.0 6.0751996181727845 0.0 9 0.0 0.0 0.0 6.285674046312199 0.0 10 0.0 0.0 0.0 9.156028840473684 0.0 11 0.0 0.0 0.0 9.849264317542534 0.0 12 0.0 0.0 0.0 12.97547464330844 0.0 13 0.0 0.0 0.0 13.314659270068423 0.0 14 0.0 0.0 0.0 13.502443146475336 0.0 15 0.0 0.0 0.0 13.97346770312934 0.0 16 0.0 0.0 0.0 14.419454409595756 0.0 17 0.0 0.0 0.0 14.876395175519242 0.0 18 0.0 0.0 0.0 15.390844753592345 0.0 19 0.0 0.0 0.0 15.81061996064363 0.0 20 0.0 0.0 0.0 16.139632960764906 0.0 21 0.0 0.0 0.0 16.501508139257393 0.0 22 0.0 0.0 0.0 16.882552921799753 0.0 23 0.0 0.0 0.0 17.220955115741376 0.0 24 0.0 0.0 0.0 17.5304072954036 0.0 25 0.0 0.0 0.0 17.79134864032737 0.0 26 0.0 0.0 0.0 18.02881700070028 0.0 27 0.0 0.0 0.0 18.28701983075978 0.0 28 0.0 0.0 0.0 18.53935441468157 0.0 29 0.0 0.0 0.0 18.816726848790946 0.0 30 0.0 0.0 0.0 19.128526326908254 0.0 31 0.0 0.0 0.0 19.429762961977676 0.0 32 0.0 0.0 0.0 19.727087432955287 0.0 33 0.0 0.0 0.0 19.989984859924963 0.0 34 3.912164091810667E-4 0.0 0.0 20.261489047896625 0.0 35 3.912164091810667E-4 0.0 0.0 20.558031086055873 0.0 36 3.912164091810667E-4 0.0 0.0 20.84244541553051 0.0 37 3.912164091810667E-4 0.0 0.0 21.10729892454609 0.0 38 3.912164091810667E-4 0.0 0.0 21.433182193393918 0.0 39 3.912164091810667E-4 0.0 0.0 21.7414607238286 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCGTTCA 20 7.0229836E-4 45.000004 16 CGTATAT 20 7.0229836E-4 45.000004 17 CAAGTGA 20 7.0229836E-4 45.000004 5 GCGAACA 20 7.0229836E-4 45.000004 33 GCGCGAC 30 2.1588567E-6 45.000004 9 ACACCCA 35 1.2069722E-7 45.000004 30 CCAATCC 20 7.0229836E-4 45.000004 13 AAGCGGT 20 7.0229836E-4 45.000004 42 GTTACTT 20 7.0229836E-4 45.000004 34 CGTTAAT 20 7.0229836E-4 45.000004 23 ATCCGCC 20 7.0229836E-4 45.000004 18 CCCAATC 20 7.0229836E-4 45.000004 12 CCCGTAA 20 7.0229836E-4 45.000004 17 CCGTAAA 20 7.0229836E-4 45.000004 18 GCACAAT 20 7.0229836E-4 45.000004 42 TGCGATT 20 7.0229836E-4 45.000004 29 GTGTCGG 20 7.0229836E-4 45.000004 2 TTGCGAT 20 7.0229836E-4 45.000004 28 CGAATGG 35 1.2069722E-7 45.000004 2 TCGTATA 20 7.0229836E-4 45.000004 16 >>END_MODULE