##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936699.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	307541
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.075921584439147	31.0	31.0	33.0	28.0	34.0
2	31.110616145489544	31.0	31.0	34.0	28.0	34.0
3	30.87182522005196	31.0	30.0	34.0	27.0	34.0
4	34.87012788538764	35.0	35.0	37.0	32.0	37.0
5	35.098448662129606	35.0	35.0	37.0	33.0	37.0
6	35.06436540168628	35.0	35.0	37.0	32.0	37.0
7	31.087828939881188	35.0	35.0	37.0	0.0	37.0
8	32.94202399029723	35.0	35.0	37.0	17.0	37.0
9	35.9528973372656	38.0	34.0	39.0	32.0	39.0
10	36.03114056337204	37.0	35.0	39.0	32.0	39.0
11	36.316725249641514	37.0	35.0	39.0	32.0	39.0
12	36.351296249930904	38.0	35.0	39.0	32.0	39.0
13	36.06983784275918	38.0	35.0	39.0	31.0	39.0
14	37.24320984844297	39.0	36.0	40.0	32.0	41.0
15	37.269934740408594	39.0	36.0	41.0	32.0	41.0
16	37.45129917636998	39.0	36.0	40.0	32.0	41.0
17	37.16321726208863	39.0	35.0	40.0	32.0	41.0
18	37.0757785140843	39.0	36.0	40.0	32.0	41.0
19	36.863143450791924	38.0	35.0	40.0	31.0	41.0
20	36.755554543946985	38.0	35.0	40.0	31.0	41.0
21	36.950354586868094	39.0	35.0	40.0	32.0	41.0
22	36.97424733612754	39.0	35.0	40.0	32.0	41.0
23	37.06298347212242	39.0	35.0	40.0	32.0	41.0
24	36.90576541014044	39.0	35.0	40.0	31.0	41.0
25	36.74872943770099	38.0	35.0	40.0	31.0	41.0
26	36.57439821031993	38.0	35.0	40.0	31.0	41.0
27	36.64538061591788	38.0	35.0	40.0	31.0	41.0
28	36.576196344552436	38.0	35.0	40.0	31.0	41.0
29	36.47433350350035	38.0	35.0	40.0	30.0	41.0
30	36.24627610627526	38.0	35.0	40.0	30.0	41.0
31	36.328843959016844	38.0	35.0	40.0	30.0	41.0
32	36.00223059689603	38.0	35.0	40.0	30.0	41.0
33	35.744469843045316	38.0	35.0	40.0	28.0	41.0
34	35.64125433682013	38.0	35.0	40.0	27.0	41.0
35	35.504446561596666	38.0	35.0	40.0	27.0	41.0
36	35.49464949388862	38.0	35.0	40.0	27.0	41.0
37	35.343707017926064	38.0	35.0	40.0	26.0	41.0
38	35.18772456355413	38.0	34.0	40.0	25.0	41.0
39	35.037312098224305	38.0	34.0	40.0	24.0	41.0
40	34.67261600892239	38.0	34.0	40.0	23.0	41.0
41	34.66895145687892	38.0	34.0	40.0	23.0	41.0
42	34.59417118367958	38.0	34.0	40.0	23.0	41.0
43	34.573851941692325	38.0	34.0	40.0	23.0	41.0
44	34.44081276967949	38.0	33.0	40.0	23.0	41.0
45	34.346070930380016	37.0	33.0	40.0	23.0	41.0
46	34.14055686884025	37.0	33.0	40.0	22.0	41.0
47	33.899723939247124	37.0	33.0	40.0	21.0	41.0
48	33.89271674345859	37.0	33.0	40.0	21.0	41.0
49	33.78641872140625	37.0	33.0	40.0	20.0	41.0
50	33.66292949557945	37.0	33.0	40.0	20.0	41.0
51	31.771633700872403	35.0	29.0	39.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	6.0
9	26.0
10	31.0
11	17.0
12	14.0
13	17.0
14	15.0
15	27.0
16	55.0
17	85.0
18	175.0
19	410.0
20	739.0
21	1109.0
22	1535.0
23	2161.0
24	3015.0
25	4049.0
26	5042.0
27	5792.0
28	6175.0
29	6894.0
30	8075.0
31	10148.0
32	12997.0
33	17301.0
34	25565.0
35	27236.0
36	28756.0
37	41892.0
38	54623.0
39	43554.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.82340891133215	17.198357292198438	19.626001086034055	14.352232710435356
2	31.007898133907347	30.744518617029925	21.451773909820155	16.795809339242574
3	29.198708464887606	30.51463056958259	26.454033771106943	13.832627194422859
4	26.547029501757493	22.371651259506862	34.32713036635766	16.75418887237799
5	30.113383256216242	26.37697087542799	24.731011474892778	18.778634393462987
6	23.23495078704953	37.92372399127271	26.204310969919458	12.637014251758302
7	61.95141460813355	17.05788821653048	17.292653662438504	3.698043512897467
8	68.9755187113263	12.827557951622712	13.048341521943415	5.14858181510758
9	63.85197420831694	7.961865247235328	13.870345742518882	14.315814801928846
10	38.9551311857606	26.034902663384717	20.77674196286024	14.233224187994445
11	33.169886291583886	23.170894287265764	25.85671503962073	17.80250438152962
12	30.232391778657153	20.268517043256022	29.842850221596468	19.656240956490354
13	23.833895318022638	23.784796173518327	34.39834038388378	17.98296812457526
14	19.740132210014274	30.680462117246154	27.357978285822053	22.221427386917515
15	16.479103599194904	24.888063705327095	40.715221710276026	17.917610985201975
16	19.522275078769987	24.47836223462888	32.40803665202363	23.591326034577502
17	19.165899831242015	24.128815344945878	33.01543534032861	23.689849483483503
18	20.474993578092025	25.19241336927434	31.632855456670818	22.699737595962816
19	21.95284531168202	27.72475865006617	28.16665095060496	22.15574508764685
20	25.78778114137627	24.670206574082805	32.533873532309514	17.00813875223141
21	23.652781255182237	29.561912070260547	27.657125391411224	19.128181283145988
22	20.412237717897778	24.332690600602845	30.292546359672368	24.96252532182701
23	21.308703554973157	28.993532569641122	29.972589020650904	19.725174854734817
24	23.535073372330846	22.735180024777186	29.12197072910604	24.607775873785933
25	20.000585287815284	30.44277023226171	27.481539046826274	22.075105433096727
26	19.55446590861056	26.196181972484972	31.06512627584615	23.18422584305832
27	22.572925235984794	27.430488942937693	26.950552934405493	23.046032886672023
28	18.140995834701716	26.627018836512857	32.94845240146842	22.283532927317008
29	23.043431607492984	24.145073339814854	30.674609239093325	22.136885813598838
30	19.86824520958181	26.80553162017422	30.434641234827225	22.891581935416742
31	25.36995067324357	24.4764112752446	24.80644857108483	25.347189480427
32	26.75675763556729	29.3863257256756	25.382306749343993	18.474609889413117
33	22.285809046598665	28.21477461541713	24.322285483886702	25.177130854097502
34	25.21419908239877	25.828426128548713	29.40095792105768	19.556416867994837
35	20.468165220247055	27.530963351227967	25.899961305972212	26.10091012255277
36	20.244455210849935	30.076965347709738	27.292621146448763	22.38595829499156
37	23.728218351374288	27.17166166462358	27.47178424990489	21.62833573409724
38	20.118618330564058	30.1458992459542	23.454433717780717	26.281048705701025
39	27.8704302840922	24.770355822475704	26.899827990414288	20.45938590301781
40	22.284833566906524	28.88135240504518	28.72267437512397	20.111139652924326
41	21.098975421163356	29.49037689283705	26.462162768541432	22.94848491745816
42	20.916560718733436	28.659593355032335	29.270893962105866	21.15295196412836
43	23.345179992261194	27.92863390572314	25.397264104623446	23.328921997392218
44	22.805739722508545	25.21322360270663	28.623500606423207	23.357536068361618
45	20.855755817923463	24.74954558904341	26.661160625737708	27.73353796729542
46	24.669231094390668	27.947493179771154	25.05519589258018	22.328079833258005
47	19.88385288465603	25.980926120419717	33.22288735485675	20.912333640067505
48	23.057088323182924	25.552365375673485	26.70863397075512	24.68191233038847
49	19.906288917575218	23.852429432173274	32.50460914154535	23.736672508706157
50	20.69610230831011	25.060723610835627	29.844150861185987	24.399023219668273
51	19.678026669614784	24.500798267548067	27.533239470509624	28.28793559232753
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	119.0
1	349.0
2	579.0
3	2664.5
4	4750.0
5	3240.5
6	1731.0
7	1662.5
8	1594.0
9	1491.0
10	1388.0
11	1358.0
12	1328.0
13	1233.0
14	1138.0
15	1132.5
16	1127.0
17	1037.5
18	948.0
19	934.0
20	920.0
21	1114.0
22	1308.0
23	1410.0
24	1512.0
25	1855.5
26	2588.0
27	2977.0
28	3311.0
29	3645.0
30	3949.5
31	4254.0
32	5074.5
33	5895.0
34	6857.0
35	7819.0
36	8120.5
37	8422.0
38	9585.5
39	10749.0
40	11935.0
41	13121.0
42	14304.0
43	15487.0
44	17756.5
45	20026.0
46	32701.0
47	45376.0
48	35375.5
49	25375.0
50	24735.5
51	24096.0
52	21676.0
53	19256.0
54	17520.0
55	15784.0
56	14569.5
57	13355.0
58	13094.0
59	12833.0
60	12740.0
61	12647.0
62	11458.0
63	10269.0
64	8170.5
65	6072.0
66	4666.5
67	3261.0
68	2683.5
69	2106.0
70	1759.0
71	1412.0
72	1280.0
73	1148.0
74	920.5
75	537.0
76	381.0
77	289.5
78	198.0
79	163.5
80	129.0
81	85.5
82	42.0
83	32.5
84	23.0
85	16.0
86	9.0
87	13.0
88	17.0
89	16.5
90	16.0
91	10.5
92	5.0
93	3.0
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	307541.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.82110794780196
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.39667822511996	24.74602514932352
2	8.319764936335979	5.461278061516418
3	3.469798447950084	3.4164788825185624
4	2.016806816642578	2.6477533695555557
5	1.4448597544348352	2.371094898487018
6	1.0129016386163907	1.9946732412920432
7	0.7739248441628083	1.778074959762734
8	0.6399378556066141	1.680277555099967
9	0.572944361346829	1.6924201858663859
>10	6.186399238486251	40.99745962752761
>50	0.12398795952356474	2.613947407489813
>100	0.031996892780274774	2.0557145713140224
>500	0.003999611597534347	1.081022317673753
>1k	0.003999611597534347	3.2260016341023054
>5k	0.0019998057987671734	4.2377781384703015
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGC	6620	2.152558520652531	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCG	6293	2.046231234209422	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTC	4549	1.4791523731795109	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2482	0.8070468652960093	No Hit
GCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTGC	1649	0.5361886707788555	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCT	1150	0.3739338819864668	No Hit
GAACTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCT	953	0.30987738220269817	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTC	898	0.29199358784682367	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCCGAGAATCGTA	866	0.2815884711306785	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	577	0.18761726078799248	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTT	421	0.13689231679678482	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTT	393	0.1277878396701578	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGC	381	0.12388592090160336	No Hit
CCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCGTCTTCTGC	334	0.10860340572476515	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCTCCGAGAATCGTATGCCG	308	0.10014924839289721	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.10665244634048794	0.0
2	0.0	0.0	0.0	1.0580703060730114	0.0
3	0.0	0.0	0.0	1.2573933231666672	0.0
4	0.0	0.0	0.0	1.8517856155764598	0.0
5	0.0	0.0	0.0	4.347387827964401	0.0
6	0.0	0.0	0.0	4.901785452996511	0.0
7	0.0	0.0	0.0	5.683469846296917	0.0
8	0.0	0.0	0.0	6.637163825311097	0.0
9	0.0	0.0	0.0	6.868027352450568	0.0
10	0.0	0.0	0.0	9.862099687521338	0.0
11	0.0	0.0	0.0	10.603464253546681	0.0
12	0.0	0.0	0.0	13.84400779083114	0.0
13	0.0	0.0	0.0	14.240702865634175	0.0
14	0.0	0.0	0.0	14.480345710002894	0.0
15	0.0	0.0	0.0	15.027264657395275	0.0
16	0.0	0.0	0.0	15.538741175973286	0.0
17	0.0	0.0	0.0	16.05411961331985	0.0
18	0.0	0.0	0.0	16.576651568408764	0.0
19	0.0	0.0	0.0	16.997083315720506	0.0
20	0.0	0.0	0.0	17.35215792365896	0.0
21	0.0	0.0	0.0	17.734545962977293	0.0
22	0.0	0.0	0.0	18.168309266081597	0.0
23	0.0	0.0	0.0	18.56532950078201	0.0
24	0.0	0.0	0.0	18.89471647682748	0.0
25	0.0	0.0	0.0	19.171102389600087	0.0
26	0.0	0.0	0.0	19.431555467401093	0.0
27	0.0	0.0	0.0	19.726800654221712	0.0
28	0.0	0.0	0.0	20.00286140709694	0.0
29	0.0	0.0	0.0	20.294204675149004	0.0
30	0.0	0.0	0.0	20.64147544555035	0.0
31	0.0	0.0	0.0	20.96435922364823	0.0
32	0.0	0.0	0.0	21.27358628605617	0.0
33	0.0	0.0	0.0	21.56005215564754	0.0
34	0.0	0.0	0.0	21.867978578465962	0.0
35	0.0	0.0	0.0	22.1915126763586	0.0
36	0.0	0.0	0.0	22.522200291993588	0.0
37	0.0	0.0	0.0	22.831427354401526	0.0
38	0.0	0.0	0.0	23.14585697516754	0.0
39	0.0	0.0	0.0	23.479796189776323	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATTGTCG	25	3.8841194E-5	45.000004	11
TGTGATC	25	3.8841194E-5	45.000004	19
TCACTTA	60	0.0	45.000004	5
GGTGCGT	25	3.8841194E-5	45.000004	9
GTTATCA	25	3.8841194E-5	45.000004	10
TATCATG	20	7.0252194E-4	45.0	43
CGGGTCA	20	7.0252194E-4	45.0	6
TCGTTCA	20	7.0252194E-4	45.0	16
CGAACGG	20	7.0252194E-4	45.0	2
CATATTC	20	7.0252194E-4	45.0	21
GTCGATG	20	7.0252194E-4	45.0	1
CCAATTC	20	7.0252194E-4	45.0	34
CACGCGT	20	7.0252194E-4	45.0	28
CGTTCAT	20	7.0252194E-4	45.0	17
TCTAGAC	20	7.0252194E-4	45.0	36
GGGCGTA	20	7.0252194E-4	45.0	38
TACCTCC	20	7.0252194E-4	45.0	25
GCGATGC	20	7.0252194E-4	45.0	9
GGTGATC	20	7.0252194E-4	45.0	8
ACATACG	45	3.8198777E-10	45.0	17
>>END_MODULE