SRR2936698.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2936698.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	255638
Sequences flagged as poor quality	0
Sequence length	51
%GC	44

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGC	4166	1.6296481743715723	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCG	4099	1.6034392382979057	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTC	3588	1.403547203467403	TruSeq Adapter, Index 13 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3560	1.3925942152575126	No Hit
GCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGC	1639	0.6411409884289504	TruSeq Adapter, Index 13 (96% over 25bp)
GAATGACTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCT	873	0.3414985252583732	No Hit
GAACTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCT	761	0.29768657241881097	RNA PCR Primer, Index 13 (95% over 23bp)
GAATGCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTT	720	0.28164826825432837	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTC	676	0.2644364296387861	No Hit
CCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGC	576	0.2253186146034627	TruSeq Adapter, Index 13 (96% over 25bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTA	516	0.2018479255822687	No Hit
CTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGCT	504	0.1971537877780299	Illumina PCR Primer Index 4 (95% over 23bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	419	0.163903644998005	No Hit
TCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGC	409	0.15999186349447264	TruSeq Adapter, Index 13 (96% over 25bp)
GAATGAAACGGCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGC	316	0.12361229551162191	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCG	264	0.10327103169325373	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAT	30	2.1588567E-6	45.000004	6
AACCGGA	20	7.0229836E-4	45.000004	36
ACTGCCG	20	7.0229836E-4	45.000004	1
TTGTGCG	20	7.0229836E-4	45.000004	1
CGACGGT	80	0.0	45.000004	28
GCGCGAC	20	7.0229836E-4	45.000004	9
CGTGAGT	30	2.1588567E-6	45.000004	32
CGCATGG	20	7.0229836E-4	45.000004	2
GGCCGCA	20	7.0229836E-4	45.000004	37
TTGTCGC	30	2.1588567E-6	45.000004	38
GTTAGTA	20	7.0229836E-4	45.000004	34
ATTCGTA	30	2.1588567E-6	45.000004	32
ATCGTGA	30	2.1588567E-6	45.000004	30
GCCGCAC	20	7.0229836E-4	45.000004	38
AAACGTA	20	7.0229836E-4	45.000004	12
ACAGCGG	20	7.0229836E-4	45.000004	2
CGTACAT	30	2.1588567E-6	45.000004	35
TCGTACA	30	2.1588567E-6	45.000004	34
TGGATCG	20	7.0229836E-4	45.000004	2
ACGTATA	20	7.0229836E-4	45.000004	14

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content