##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936698.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	255638
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.897280529498744	31.0	30.0	33.0	28.0	34.0
2	30.9400871544919	31.0	31.0	33.0	28.0	34.0
3	30.671453383299824	31.0	30.0	33.0	27.0	34.0
4	34.661005014903886	35.0	35.0	37.0	32.0	37.0
5	34.9380960577066	35.0	35.0	37.0	32.0	37.0
6	34.91584975629601	35.0	35.0	37.0	32.0	37.0
7	31.06616778413225	35.0	35.0	37.0	0.0	37.0
8	32.96545896932381	35.0	35.0	37.0	17.0	37.0
9	35.996264248664126	38.0	34.0	39.0	32.0	39.0
10	35.83896369084408	37.0	35.0	39.0	32.0	39.0
11	36.14929314108231	37.0	35.0	39.0	32.0	39.0
12	36.20280631205063	37.0	35.0	39.0	32.0	39.0
13	35.930702790664924	37.0	35.0	39.0	31.0	39.0
14	36.87578529013683	39.0	36.0	40.0	31.0	41.0
15	37.02052120576753	39.0	35.0	40.0	31.0	41.0
16	37.14454032655552	39.0	36.0	40.0	32.0	41.0
17	37.016992778851346	38.0	35.0	40.0	32.0	41.0
18	36.917477057401484	38.0	35.0	40.0	32.0	41.0
19	36.668867695725986	38.0	35.0	40.0	31.0	41.0
20	36.55507397178823	38.0	35.0	40.0	31.0	41.0
21	36.705959207942485	38.0	35.0	40.0	31.0	41.0
22	36.81992505026639	38.0	35.0	40.0	32.0	41.0
23	36.912872890571826	38.0	35.0	40.0	32.0	41.0
24	36.798449369812	38.0	35.0	40.0	31.0	41.0
25	36.550023861867174	38.0	35.0	40.0	31.0	41.0
26	36.51630821708822	38.0	35.0	40.0	31.0	41.0
27	36.58575016233893	38.0	35.0	40.0	31.0	41.0
28	36.546076874330105	38.0	35.0	40.0	31.0	41.0
29	36.452444472261554	38.0	35.0	40.0	31.0	41.0
30	36.25785681314984	38.0	35.0	40.0	30.0	41.0
31	36.24997848520173	38.0	35.0	40.0	30.0	41.0
32	35.99218817233744	38.0	35.0	40.0	30.0	41.0
33	35.7632316009357	38.0	35.0	40.0	29.0	41.0
34	35.658352827044496	38.0	35.0	40.0	29.0	41.0
35	35.49989829368091	38.0	35.0	40.0	27.0	41.0
36	35.429916522582715	38.0	35.0	40.0	27.0	41.0
37	35.28251277196661	38.0	34.0	40.0	26.0	41.0
38	35.118331390481856	38.0	34.0	40.0	25.0	41.0
39	34.9109678529796	38.0	34.0	40.0	24.0	41.0
40	34.56727481829775	38.0	34.0	40.0	23.0	41.0
41	34.41635828789147	38.0	33.0	40.0	23.0	41.0
42	34.455765574758054	38.0	33.0	40.0	23.0	41.0
43	34.36836855240614	38.0	33.0	40.0	22.0	41.0
44	34.1231467935127	37.0	33.0	40.0	21.0	41.0
45	34.06648072665253	37.0	33.0	40.0	21.0	41.0
46	33.783651100384134	37.0	33.0	40.0	20.0	41.0
47	33.60292679492094	37.0	33.0	40.0	19.0	41.0
48	33.51584662687081	37.0	33.0	40.0	18.0	41.0
49	33.4062033031083	37.0	32.0	40.0	18.0	41.0
50	33.2300088406262	36.0	32.0	40.0	17.0	41.0
51	31.306992700615716	35.0	28.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	11.0
10	15.0
11	19.0
12	3.0
13	4.0
14	7.0
15	15.0
16	33.0
17	68.0
18	130.0
19	291.0
20	514.0
21	767.0
22	1152.0
23	1703.0
24	2507.0
25	3557.0
26	4472.0
27	4984.0
28	5441.0
29	6138.0
30	7532.0
31	9321.0
32	11723.0
33	15445.0
34	22096.0
35	24167.0
36	24793.0
37	34079.0
38	43767.0
39	30876.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.60868884907565	19.494754301003763	22.74192412708596	15.154632722834632
2	30.457521964653147	30.102723382282758	23.962399956188047	15.47735469687605
3	25.85844045095017	28.59825221602422	31.332587486993326	14.21071984603228
4	25.368685406707925	22.72627700107183	35.51623780502116	16.388799787199087
5	27.064442688489194	25.826755020771557	28.613508163887996	18.49529412685125
6	21.380232985706353	36.4335505675995	28.522363654855692	13.66385279183846
7	61.89377166149007	16.410314585468512	17.461410275467653	4.234503477573757
8	71.15100258959936	8.600051635515847	14.128963612608453	6.119982162276344
9	67.71137311354337	5.052456989962368	15.128814964911319	12.107354931582941
10	38.10935776371275	23.31460893920309	23.05916960702243	15.516863690061728
11	27.892175654636635	24.911006970794638	28.728514540091847	18.468302834476876
12	26.187812453547593	20.28923712437118	34.566066077813154	18.956884344268065
13	20.868180786893966	26.149085816662627	37.20182445489325	15.780908941550162
14	16.734601272111345	30.81779704112847	32.171273441350664	20.276328245409523
15	13.781597415094781	25.038922225960146	44.12293946909301	17.056540889852055
16	16.514759151612825	24.99941323277447	34.479224528434735	24.006603087177965
17	16.98573764463812	24.44746086262606	33.35067556466566	25.21612592807016
18	19.040205290293304	24.76587987701359	34.996753221352066	21.197161611341038
19	18.617341709761458	27.605833248578065	30.75012322111736	23.02670182054311
20	18.447179214357803	27.00420125333479	34.725275585006926	19.82334394730048
21	18.525023666278095	29.751445403265553	33.3612373747252	18.362293555731153
22	17.02289956892168	23.139752305995195	32.96692979916914	26.870418325913985
23	14.511927021804269	28.569305032898086	34.34583277916429	22.57293516613336
24	19.72750530046394	24.086794608000375	32.14193508007417	24.04376501146152
25	16.83317816600036	31.172204445348502	29.71584819158341	22.27876919706773
26	15.074050023861869	25.169575728178128	31.85989563366949	27.896478614290523
27	17.974636008731096	27.18531673694834	32.261244415931905	22.578802838388658
28	14.024127868313787	24.011688403132556	36.16442000015647	25.799763728397185
29	16.688833428520017	24.374701726660355	32.16853519429819	26.767929650521438
30	17.68203475226688	27.389902909583082	34.44910381085754	20.478958527292498
31	21.425218472996974	25.7536047066555	27.692674797956485	25.128502022391036
32	20.259116406793982	30.700834774172854	27.922296372213832	21.11775244681933
33	18.841877968064217	27.206831535217766	27.272940642627464	26.67834985409055
34	20.421455339190576	24.15877138766537	29.400558602398704	26.01921467074535
35	15.999186349447264	25.430882732614084	32.418498032373904	26.151432885564745
36	19.732199438268175	24.265563022711802	28.93896838498189	27.06326915403813
37	16.20768430358554	27.916037521808185	34.535554182085605	21.340723992520676
38	16.17639005155728	28.939359563132243	27.958284762046333	26.925965623264148
39	21.56173964747025	26.36775440271008	28.000140824134128	24.070365125685537
40	20.808721708040277	24.663782379771394	32.5874869933265	21.940008918861828
41	18.67562725416409	23.459344854833788	28.959309648800257	28.90571824220186
42	18.53636783263834	21.68496076483152	34.37790938749325	25.400762015036886
43	20.42106416104022	22.815856797502718	28.510628310345098	28.25245073111196
44	19.704425789593095	23.920152715949897	30.119935220898302	26.255486273558702
45	18.097857126092364	24.557381922875315	28.148788521268358	29.195972429763962
46	23.448391866623897	25.32995876982295	28.70074089141677	22.52090847213638
47	16.857822389472613	22.609705912266563	36.398344534067704	24.134127164193117
48	18.532456051134808	24.62701163363819	30.900726809003352	25.939805506223646
49	18.051698104350685	21.38375358905953	35.1821716646195	25.382376641970282
50	19.188070631126827	21.65092826575079	33.25014278002488	25.910858323097507
51	18.318872781041943	21.598510393603455	29.497961961836662	30.584654863517947
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	89.0
1	222.5
2	356.0
3	2663.5
4	4971.0
5	3172.5
6	1374.0
7	1280.0
8	1186.0
9	1201.5
10	1217.0
11	1215.0
12	1213.0
13	1189.5
14	1166.0
15	1138.5
16	1111.0
17	1068.0
18	1025.0
19	942.0
20	859.0
21	863.0
22	867.0
23	920.0
24	973.0
25	1103.5
26	1394.0
27	1554.0
28	1974.5
29	2395.0
30	2967.5
31	3540.0
32	4581.0
33	5622.0
34	6670.5
35	7719.0
36	7892.5
37	8066.0
38	8571.5
39	9077.0
40	10683.0
41	12289.0
42	13919.0
43	15549.0
44	19040.0
45	22531.0
46	32316.0
47	42101.0
48	35879.0
49	29657.0
50	27291.0
51	24925.0
52	20625.0
53	16325.0
54	13487.0
55	10649.0
56	9291.0
57	7933.0
58	7072.5
59	6212.0
60	5253.5
61	4295.0
62	3636.5
63	2978.0
64	2357.5
65	1737.0
66	1484.5
67	1232.0
68	923.0
69	614.0
70	486.5
71	359.0
72	345.5
73	332.0
74	245.0
75	97.0
76	36.0
77	35.0
78	34.0
79	26.5
80	19.0
81	20.5
82	22.0
83	17.5
84	13.0
85	12.0
86	11.0
87	9.5
88	8.0
89	5.0
90	2.0
91	1.0
92	0.0
93	1.0
94	2.0
95	1.5
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	255638.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.591078973796655
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.53994010890767	31.068387536103376
2	10.342948649761558	8.396628909287896
3	3.7851248036127148	4.6092689948736085
4	1.9117618307242783	3.1040190180007703
5	1.22040881357919	2.4768855266155185
6	0.8828359574666359	2.1501158442261357
7	0.6141891764141167	1.7451420955273578
8	0.5123235624807366	1.6636612947833942
9	0.4264369528988707	1.5578582429216938
>10	3.6391949708968996	30.24263754093528
>50	0.08888264335920437	2.312262137836584
>100	0.023968353265403426	1.8854016835690661
>500	0.006990769702409333	1.8752672948162759
>1k	0.00499340693029238	6.912463880503056
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGC	4166	1.6296481743715723	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCG	4099	1.6034392382979057	No Hit
GAATCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTC	3588	1.403547203467403	TruSeq Adapter, Index 13 (95% over 22bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3560	1.3925942152575126	No Hit
GCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGC	1639	0.6411409884289504	TruSeq Adapter, Index 13 (96% over 25bp)
GAATGACTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCT	873	0.3414985252583732	No Hit
GAACTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCT	761	0.29768657241881097	RNA PCR Primer, Index 13 (95% over 23bp)
GAATGCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTT	720	0.28164826825432837	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTC	676	0.2644364296387861	No Hit
CCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGC	576	0.2253186146034627	TruSeq Adapter, Index 13 (96% over 25bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTA	516	0.2018479255822687	No Hit
CTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGCT	504	0.1971537877780299	Illumina PCR Primer Index 4 (95% over 23bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	419	0.163903644998005	No Hit
TCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCGTCTTCTGC	409	0.15999186349447264	TruSeq Adapter, Index 13 (96% over 25bp)
GAATGAAACGGCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGC	316	0.12361229551162191	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCCTCAAGTCTCGTATGCCG	264	0.10327103169325373	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.2773453086004428	0.0
2	0.0	0.0	0.0	1.8373637722091394	0.0
3	0.0	0.0	0.0	2.2895657140174777	0.0
4	0.0	0.0	0.0	3.2162667522042887	0.0
5	0.0	0.0	0.0	6.348821380232986	0.0
6	0.0	0.0	0.0	7.604503242866866	0.0
7	0.0	0.0	0.0	8.825761428269663	0.0
8	0.0	0.0	0.0	10.64239275851008	0.0
9	0.0	0.0	0.0	11.148577285067166	0.0
10	0.0	0.0	0.0	14.37149406582746	0.0
11	0.0	0.0	0.0	16.076248445066852	0.0
12	0.0	0.0	0.0	19.468154186779742	0.0
13	0.0	0.0	0.0	20.21530445395442	0.0
14	0.0	0.0	0.0	20.578708955632575	0.0
15	0.0	0.0	0.0	21.194423364288564	0.0
16	0.0	0.0	0.0	22.119950868024315	0.0
17	0.0	0.0	0.0	23.193734890743944	0.0
18	0.0	0.0	0.0	24.519437642291052	0.0
19	0.0	0.0	0.0	25.304532190049994	0.0
20	0.0	0.0	0.0	25.982052746461793	0.0
21	0.0	0.0	0.0	26.816826919315595	0.0
22	0.0	0.0	0.0	27.764651577621482	0.0
23	0.0	0.0	0.0	28.648323019269437	0.0
24	0.0	0.0	0.0	29.292984611051565	0.0
25	0.0	0.0	0.0	29.79916913760865	0.0
26	0.0	0.0	0.0	30.311612514571387	0.0
27	0.0	0.0	0.0	30.851047183908495	0.0
28	0.0	0.0	0.0	31.347843434857104	0.0
29	0.0	0.0	0.0	31.91857235622247	0.0
30	3.911781503532339E-4	0.0	0.0	32.53506912117917	0.0
31	3.911781503532339E-4	0.0	0.0	33.05181545779579	0.0
32	3.911781503532339E-4	0.0	0.0	33.55878234065358	0.0
33	3.911781503532339E-4	0.0	0.0	34.04775502859512	0.0
34	3.911781503532339E-4	0.0	0.0	34.575454353421634	0.0
35	3.911781503532339E-4	0.0	0.0	35.17513045791314	0.0
36	3.911781503532339E-4	0.0	0.0	35.63945892238243	0.0
37	3.911781503532339E-4	0.0	0.0	36.15268465564587	0.0
38	3.911781503532339E-4	0.0	0.0	36.64400441248954	0.0
39	3.911781503532339E-4	0.0	0.0	37.31213669329286	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTAT	30	2.1588567E-6	45.000004	6
AACCGGA	20	7.0229836E-4	45.000004	36
ACTGCCG	20	7.0229836E-4	45.000004	1
TTGTGCG	20	7.0229836E-4	45.000004	1
CGACGGT	80	0.0	45.000004	28
GCGCGAC	20	7.0229836E-4	45.000004	9
CGTGAGT	30	2.1588567E-6	45.000004	32
CGCATGG	20	7.0229836E-4	45.000004	2
GGCCGCA	20	7.0229836E-4	45.000004	37
TTGTCGC	30	2.1588567E-6	45.000004	38
GTTAGTA	20	7.0229836E-4	45.000004	34
ATTCGTA	30	2.1588567E-6	45.000004	32
ATCGTGA	30	2.1588567E-6	45.000004	30
GCCGCAC	20	7.0229836E-4	45.000004	38
AAACGTA	20	7.0229836E-4	45.000004	12
ACAGCGG	20	7.0229836E-4	45.000004	2
CGTACAT	30	2.1588567E-6	45.000004	35
TCGTACA	30	2.1588567E-6	45.000004	34
TGGATCG	20	7.0229836E-4	45.000004	2
ACGTATA	20	7.0229836E-4	45.000004	14
>>END_MODULE