##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936695.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	409364
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.12104874879081	31.0	31.0	33.0	28.0	34.0
2	31.16544688834387	31.0	31.0	34.0	28.0	34.0
3	30.937776648654985	31.0	31.0	34.0	27.0	34.0
4	34.91265719506356	35.0	35.0	37.0	32.0	37.0
5	35.154143989212535	35.0	35.0	37.0	33.0	37.0
6	35.126171329183805	36.0	35.0	37.0	32.0	37.0
7	30.987991127700532	35.0	35.0	37.0	0.0	37.0
8	33.04374835110073	35.0	35.0	37.0	17.0	37.0
9	36.335991928943436	39.0	35.0	39.0	32.0	39.0
10	36.06604146920589	37.0	35.0	39.0	32.0	39.0
11	36.3569268426144	37.0	35.0	39.0	32.0	39.0
12	36.333842252860535	38.0	35.0	39.0	32.0	39.0
13	36.22738198766868	38.0	35.0	39.0	32.0	39.0
14	37.130116473358676	39.0	36.0	40.0	32.0	41.0
15	37.37711669809754	39.0	36.0	41.0	32.0	41.0
16	37.34596349459161	39.0	36.0	40.0	32.0	41.0
17	37.27733997127251	39.0	36.0	40.0	32.0	41.0
18	37.205611631701856	39.0	36.0	40.0	32.0	41.0
19	37.05215163033388	39.0	35.0	40.0	32.0	41.0
20	37.03842545998182	39.0	35.0	40.0	31.0	41.0
21	37.013318220459055	39.0	35.0	40.0	31.0	41.0
22	37.13604518228276	39.0	35.0	40.0	32.0	41.0
23	37.22145327874459	39.0	35.0	40.0	32.0	41.0
24	37.167872602378324	39.0	35.0	40.0	32.0	41.0
25	36.83498548968644	39.0	35.0	40.0	31.0	41.0
26	36.85604498685766	39.0	35.0	40.0	31.0	41.0
27	36.86366900851076	39.0	35.0	40.0	31.0	41.0
28	36.78725290939115	39.0	35.0	40.0	31.0	41.0
29	36.714141937249	39.0	35.0	40.0	31.0	41.0
30	36.491909400924364	39.0	35.0	40.0	30.0	41.0
31	36.45865049198269	39.0	35.0	40.0	30.0	41.0
32	36.34400680079342	38.0	35.0	40.0	30.0	41.0
33	36.199143060943314	38.0	35.0	40.0	30.0	41.0
34	36.08023665979422	38.0	35.0	40.0	29.0	41.0
35	36.03062311292639	38.0	35.0	40.0	29.0	41.0
36	35.96584457841921	38.0	35.0	40.0	29.0	41.0
37	35.8630802904017	38.0	35.0	40.0	29.0	41.0
38	35.663758904056046	38.0	34.0	40.0	28.0	41.0
39	35.56230640701185	38.0	34.0	40.0	27.0	41.0
40	35.420325187363815	38.0	34.0	40.0	27.0	41.0
41	35.228024447679815	38.0	34.0	40.0	26.0	41.0
42	35.23641551284432	38.0	34.0	40.0	26.0	41.0
43	35.1351535552711	38.0	34.0	40.0	26.0	41.0
44	34.94153858180006	38.0	34.0	40.0	26.0	41.0
45	34.85437165945223	37.0	34.0	40.0	26.0	41.0
46	34.71683636079381	37.0	33.0	40.0	25.0	41.0
47	34.67405536393039	37.0	33.0	40.0	25.0	41.0
48	34.56696729560978	37.0	33.0	40.0	25.0	41.0
49	34.402326535796995	36.0	33.0	40.0	24.0	41.0
50	34.205237881201086	36.0	33.0	40.0	24.0	41.0
51	32.596539998632025	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	18.0
9	41.0
10	55.0
11	38.0
12	32.0
13	19.0
14	38.0
15	40.0
16	65.0
17	116.0
18	263.0
19	465.0
20	793.0
21	1133.0
22	1751.0
23	2318.0
24	3214.0
25	4310.0
26	5436.0
27	6223.0
28	6919.0
29	8138.0
30	10023.0
31	12552.0
32	16140.0
33	22215.0
34	33784.0
35	35430.0
36	41063.0
37	60443.0
38	76273.0
39	60012.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.46589343469382	20.16884728505682	21.56125111148025	12.804008168769116
2	33.917491523436354	23.33790953772193	24.988274494093275	17.75632444474844
3	32.95160297437	23.97157541943112	26.21407842409201	16.86274318210688
4	28.873814013933806	25.057650404041393	26.131022757252715	19.937512824772085
5	25.32464994479241	30.243743954036017	25.28214498588054	19.149461115291036
6	26.75174172618989	34.546760340430524	25.054230464818595	13.647267468560987
7	70.12023529181853	17.621725408194173	8.575497601156917	3.6825416988303807
8	81.37183533481205	5.820247994449927	8.543496741286484	4.264419929451539
9	75.45900469997362	7.860730303592891	10.639430922113327	6.040834074320165
10	41.14040316197809	27.373926383365415	17.633450914100898	13.852219540555593
11	33.171456210121065	26.633998104376545	23.22993717083085	16.96460851467154
12	30.3500063513157	23.942017373291254	27.941147731603174	17.766828543789877
13	26.91858590398765	24.447679815518708	29.569527364399413	19.064206916094236
14	21.8585415424903	27.4005530530286	30.388114245512547	20.352791158968547
15	20.43584682580784	25.520807887356973	33.9736762392394	20.069669047595784
16	25.423339619507335	26.509658885490662	29.081941743778156	18.985059751223847
17	25.614856215983817	25.19957788178736	26.883165104894424	22.3024007973344
18	27.45576064333942	24.160160639430924	28.847431625643683	19.536647091585973
19	26.32473788608671	27.003351540438338	26.013279135439365	20.658631438035588
20	26.579279076811837	27.003351540438338	27.01971839243314	19.397650990316688
21	26.64254795243353	27.039505183650736	28.290958657820426	18.02698820609531
22	24.576904661865722	24.479192112643027	29.139103585073435	21.80479964041782
23	22.84617113375871	25.79928865264166	29.374346547327075	21.98019366627256
24	22.365913954329155	25.336131169326077	29.800861824684144	22.497093051660624
25	23.844060542695498	26.362601498910504	27.385163326526023	22.408174631867972
26	21.98923207707566	26.202841480931394	27.695156388935033	24.112770053057915
27	22.293850949277417	26.305439657615224	28.94343420525498	22.457275187852378
28	20.67621969689567	26.820629073391896	29.704126400953673	22.799024828758757
29	22.638531966660477	27.3824762314224	28.902394934581448	21.076596867335674
30	25.31952003595822	25.70548460538787	28.10237343782062	20.872621920833293
31	25.582122511994214	26.817697696915214	26.643525077925755	20.956654713164813
32	26.810369255723515	27.043413685619644	25.910436677382474	20.235780381274367
33	25.48709705787514	27.321405888158218	26.116610156242366	21.074886897724273
34	22.74650433355156	27.01678701595646	29.335994371757163	20.900714278734817
35	22.74772574041684	29.02819984170567	27.45918058256222	20.76489383531527
36	24.31405790445667	29.661621442041803	25.465844578419205	20.55847607508232
37	23.727294046374375	29.299596447171712	25.80588424971419	21.167225256739723
38	22.832735658240587	29.405858844451394	25.51885363637252	22.2425518609355
39	23.71630138458682	26.6178755337548	26.750276037951554	22.915547043706823
40	25.161714268963564	26.493292033495862	26.996023099246635	21.34897059829394
41	21.95405555935549	26.84359152245923	25.967598518677754	25.23475439950753
42	23.284167635649446	26.758337323262428	26.791071027252027	23.1664240138361
43	23.068222901867287	26.722427961423083	26.97819055901349	23.231158577696135
44	22.75676415121994	25.220586079870237	27.496311351266844	24.52633841764298
45	22.688853929510167	26.037462991371985	26.19673444660498	25.076948632512874
46	22.291652416919906	28.011012204297398	26.33597482924732	23.361360549535377
47	21.89518374844881	26.769574266423035	28.397953899219274	22.937288085908875
48	21.26420496184325	27.68465228989359	27.676835285955775	23.374307462307385
49	23.204043345286834	26.289072805620428	28.408457998260715	22.098425850832022
50	21.454988714200564	26.998221631604146	28.40748087276849	23.1393087814268
51	21.218768626454697	26.524315767874068	26.961823707018695	25.295091898652544
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	106.0
1	436.0
2	766.0
3	1685.5
4	2605.0
5	1901.5
6	1198.0
7	1172.0
8	1146.0
9	1066.5
10	987.0
11	1009.0
12	1031.0
13	991.0
14	951.0
15	974.5
16	998.0
17	965.0
18	932.0
19	1018.0
20	1104.0
21	1193.5
22	1283.0
23	1613.5
24	1944.0
25	2242.5
26	3064.5
27	3588.0
28	4183.0
29	4778.0
30	5517.5
31	6257.0
32	7738.5
33	9220.0
34	10721.5
35	12223.0
36	12866.5
37	13510.0
38	14999.0
39	16488.0
40	17851.0
41	19214.0
42	20208.0
43	21202.0
44	23787.0
45	26372.0
46	29689.0
47	33006.0
48	33113.5
49	33221.0
50	33201.5
51	33182.0
52	31375.0
53	29568.0
54	27467.5
55	25367.0
56	24825.5
57	24284.0
58	22466.5
59	20649.0
60	18962.0
61	17275.0
62	15390.0
63	13505.0
64	11717.0
65	9929.0
66	8385.0
67	6841.0
68	5796.0
69	4751.0
70	3811.5
71	2872.0
72	2499.0
73	2126.0
74	1631.5
75	856.0
76	575.0
77	444.5
78	314.0
79	243.5
80	173.0
81	118.0
82	63.0
83	46.0
84	29.0
85	23.5
86	18.0
87	16.5
88	15.0
89	14.5
90	14.0
91	9.0
92	4.0
93	2.5
94	1.0
95	0.5
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	409364.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.59076101763154
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.46272867527749	25.757224523272633
2	8.505179151986786	5.884012389170341
3	3.4715537948908115	3.6025106303675987
4	2.146640421008029	2.970157031755068
5	1.4283912331059607	2.4704569892024164
6	1.0608747255495998	2.2017878460679
7	0.8034938546626168	1.9455424734039106
8	0.6515799331629918	1.8030916601540308
9	0.5679622187228647	1.7681620837397751
>10	6.801173725832029	47.67521779556224
>50	0.0777918960428393	1.7718227500881572
>100	0.01980157353817728	1.3713707492743628
>500	0.0021215971648047083	0.4772644188073995
>1k	7.071990549349028E-4	0.30137865913414463
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1232	0.30095465160590573	No Hit
GCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCTGC	774	0.18907378274591805	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTC	669	0.1634242385749602	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGC	508	0.12409493751282477	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCG	484	0.11823218455946298	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	459	0.11212515023304444	No Hit
GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG	411	0.10039964432632083	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTT	410	0.10015536295326409	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.09526973549212925	0.0
2	0.0	0.0	0.0	0.5234949824605974	0.0
3	0.0	0.0	0.0	0.6918048484966924	0.0
4	0.0	0.0	0.0	0.9338876891959235	0.0
5	0.0	0.0	0.0	1.610302811190041	0.0
6	0.0	0.0	0.0	2.08420867492012	0.0
7	0.0	0.0	0.0	2.394446018702182	0.0
8	0.0	0.0	0.0	2.9150096246860984	0.0
9	0.0	0.0	0.0	3.0987092172247683	0.0
10	0.0	0.0	0.0	3.7084355243743956	0.0
11	0.0	0.0	0.0	4.37385798458096	0.0
12	0.0	0.0	0.0	5.070792741911844	0.0
13	0.0	0.0	0.0	5.4281763906938565	0.0
14	0.0	0.0	0.0	5.638502652895712	0.0
15	0.0	0.0	0.0	5.869837113180446	0.0
16	0.0	0.0	0.0	6.268748595382105	0.0
17	0.0	0.0	0.0	6.715294945329829	0.0
18	0.0	0.0	0.0	7.234148581702348	0.0
19	0.0	0.0	0.0	7.528996198981835	0.0
20	0.0	0.0	0.0	7.862684554577345	0.0
21	0.0	0.0	0.0	8.24913768675311	0.0
22	0.0	0.0	0.0	8.62313246890298	0.0
23	0.0	0.0	0.0	9.011539852063201	0.0
24	0.0	0.0	0.0	9.31444875465356	0.0
25	0.0	0.0	0.0	9.591463831699905	0.0
26	0.0	0.0	0.0	9.866524657761797	0.0
27	0.0	0.0	0.0	10.185311849600843	0.0
28	0.0	0.0	0.0	10.459884112916622	0.0
29	0.0	0.0	0.0	10.785755464574315	0.0
30	0.0	0.0	0.0	11.209339365454705	0.0
31	0.0	0.0	0.0	11.534477872993229	0.0
32	0.0	0.0	0.0	11.831035459884113	0.0
33	0.0	0.0	0.0	12.152998309572899	0.0
34	0.0	0.0	0.0	12.504030642655437	0.0
35	0.0	0.0	0.0	12.89292658856177	0.0
36	0.0	0.0	0.0	13.202919650970774	0.0
37	0.0	0.0	0.0	13.543203603638815	0.0
38	0.0	0.0	0.0	13.939672272109908	0.0
39	0.0	0.0	0.0	14.542558700813945	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCAAGCG	30	2.1620635E-6	45.000004	17
CGGGTAC	35	1.2093733E-7	45.0	6
TCCGCGC	20	7.027959E-4	45.0	41
ACGCATT	20	7.027959E-4	45.0	43
ATTACTC	20	7.027959E-4	45.0	28
TAAGTCG	20	7.027959E-4	45.0	35
TTCCGCG	20	7.027959E-4	45.0	40
ACGGATA	20	7.027959E-4	45.0	15
CCGAATT	20	7.027959E-4	45.0	23
CGGTCTA	25	3.886391E-5	45.0	31
TCCCCCG	20	7.027959E-4	45.0	38
ATCGGAA	20	7.027959E-4	45.0	18
TACGAAT	65	0.0	41.53846	12
CGTTTTT	710	0.0	41.514084	1
GCTACGA	60	3.6379788E-12	41.250004	10
CGTTTTA	60	3.6379788E-12	41.250004	1
ACGTCAT	40	3.4509503E-7	39.375	35
CGCATGG	35	6.238437E-6	38.571426	2
TAGGCTC	35	6.238437E-6	38.571426	41
CGATGCA	35	6.238437E-6	38.571426	10
>>END_MODULE