##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2936695.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 409364 Sequences flagged as poor quality 0 Sequence length 51 %GC 48 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.12104874879081 31.0 31.0 33.0 28.0 34.0 2 31.16544688834387 31.0 31.0 34.0 28.0 34.0 3 30.937776648654985 31.0 31.0 34.0 27.0 34.0 4 34.91265719506356 35.0 35.0 37.0 32.0 37.0 5 35.154143989212535 35.0 35.0 37.0 33.0 37.0 6 35.126171329183805 36.0 35.0 37.0 32.0 37.0 7 30.987991127700532 35.0 35.0 37.0 0.0 37.0 8 33.04374835110073 35.0 35.0 37.0 17.0 37.0 9 36.335991928943436 39.0 35.0 39.0 32.0 39.0 10 36.06604146920589 37.0 35.0 39.0 32.0 39.0 11 36.3569268426144 37.0 35.0 39.0 32.0 39.0 12 36.333842252860535 38.0 35.0 39.0 32.0 39.0 13 36.22738198766868 38.0 35.0 39.0 32.0 39.0 14 37.130116473358676 39.0 36.0 40.0 32.0 41.0 15 37.37711669809754 39.0 36.0 41.0 32.0 41.0 16 37.34596349459161 39.0 36.0 40.0 32.0 41.0 17 37.27733997127251 39.0 36.0 40.0 32.0 41.0 18 37.205611631701856 39.0 36.0 40.0 32.0 41.0 19 37.05215163033388 39.0 35.0 40.0 32.0 41.0 20 37.03842545998182 39.0 35.0 40.0 31.0 41.0 21 37.013318220459055 39.0 35.0 40.0 31.0 41.0 22 37.13604518228276 39.0 35.0 40.0 32.0 41.0 23 37.22145327874459 39.0 35.0 40.0 32.0 41.0 24 37.167872602378324 39.0 35.0 40.0 32.0 41.0 25 36.83498548968644 39.0 35.0 40.0 31.0 41.0 26 36.85604498685766 39.0 35.0 40.0 31.0 41.0 27 36.86366900851076 39.0 35.0 40.0 31.0 41.0 28 36.78725290939115 39.0 35.0 40.0 31.0 41.0 29 36.714141937249 39.0 35.0 40.0 31.0 41.0 30 36.491909400924364 39.0 35.0 40.0 30.0 41.0 31 36.45865049198269 39.0 35.0 40.0 30.0 41.0 32 36.34400680079342 38.0 35.0 40.0 30.0 41.0 33 36.199143060943314 38.0 35.0 40.0 30.0 41.0 34 36.08023665979422 38.0 35.0 40.0 29.0 41.0 35 36.03062311292639 38.0 35.0 40.0 29.0 41.0 36 35.96584457841921 38.0 35.0 40.0 29.0 41.0 37 35.8630802904017 38.0 35.0 40.0 29.0 41.0 38 35.663758904056046 38.0 34.0 40.0 28.0 41.0 39 35.56230640701185 38.0 34.0 40.0 27.0 41.0 40 35.420325187363815 38.0 34.0 40.0 27.0 41.0 41 35.228024447679815 38.0 34.0 40.0 26.0 41.0 42 35.23641551284432 38.0 34.0 40.0 26.0 41.0 43 35.1351535552711 38.0 34.0 40.0 26.0 41.0 44 34.94153858180006 38.0 34.0 40.0 26.0 41.0 45 34.85437165945223 37.0 34.0 40.0 26.0 41.0 46 34.71683636079381 37.0 33.0 40.0 25.0 41.0 47 34.67405536393039 37.0 33.0 40.0 25.0 41.0 48 34.56696729560978 37.0 33.0 40.0 25.0 41.0 49 34.402326535796995 36.0 33.0 40.0 24.0 41.0 50 34.205237881201086 36.0 33.0 40.0 24.0 41.0 51 32.596539998632025 35.0 31.0 39.0 20.0 40.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 3.0 8 18.0 9 41.0 10 55.0 11 38.0 12 32.0 13 19.0 14 38.0 15 40.0 16 65.0 17 116.0 18 263.0 19 465.0 20 793.0 21 1133.0 22 1751.0 23 2318.0 24 3214.0 25 4310.0 26 5436.0 27 6223.0 28 6919.0 29 8138.0 30 10023.0 31 12552.0 32 16140.0 33 22215.0 34 33784.0 35 35430.0 36 41063.0 37 60443.0 38 76273.0 39 60012.0 40 1.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 45.46589343469382 20.16884728505682 21.56125111148025 12.804008168769116 2 33.917491523436354 23.33790953772193 24.988274494093275 17.75632444474844 3 32.95160297437 23.97157541943112 26.21407842409201 16.86274318210688 4 28.873814013933806 25.057650404041393 26.131022757252715 19.937512824772085 5 25.32464994479241 30.243743954036017 25.28214498588054 19.149461115291036 6 26.75174172618989 34.546760340430524 25.054230464818595 13.647267468560987 7 70.12023529181853 17.621725408194173 8.575497601156917 3.6825416988303807 8 81.37183533481205 5.820247994449927 8.543496741286484 4.264419929451539 9 75.45900469997362 7.860730303592891 10.639430922113327 6.040834074320165 10 41.14040316197809 27.373926383365415 17.633450914100898 13.852219540555593 11 33.171456210121065 26.633998104376545 23.22993717083085 16.96460851467154 12 30.3500063513157 23.942017373291254 27.941147731603174 17.766828543789877 13 26.91858590398765 24.447679815518708 29.569527364399413 19.064206916094236 14 21.8585415424903 27.4005530530286 30.388114245512547 20.352791158968547 15 20.43584682580784 25.520807887356973 33.9736762392394 20.069669047595784 16 25.423339619507335 26.509658885490662 29.081941743778156 18.985059751223847 17 25.614856215983817 25.19957788178736 26.883165104894424 22.3024007973344 18 27.45576064333942 24.160160639430924 28.847431625643683 19.536647091585973 19 26.32473788608671 27.003351540438338 26.013279135439365 20.658631438035588 20 26.579279076811837 27.003351540438338 27.01971839243314 19.397650990316688 21 26.64254795243353 27.039505183650736 28.290958657820426 18.02698820609531 22 24.576904661865722 24.479192112643027 29.139103585073435 21.80479964041782 23 22.84617113375871 25.79928865264166 29.374346547327075 21.98019366627256 24 22.365913954329155 25.336131169326077 29.800861824684144 22.497093051660624 25 23.844060542695498 26.362601498910504 27.385163326526023 22.408174631867972 26 21.98923207707566 26.202841480931394 27.695156388935033 24.112770053057915 27 22.293850949277417 26.305439657615224 28.94343420525498 22.457275187852378 28 20.67621969689567 26.820629073391896 29.704126400953673 22.799024828758757 29 22.638531966660477 27.3824762314224 28.902394934581448 21.076596867335674 30 25.31952003595822 25.70548460538787 28.10237343782062 20.872621920833293 31 25.582122511994214 26.817697696915214 26.643525077925755 20.956654713164813 32 26.810369255723515 27.043413685619644 25.910436677382474 20.235780381274367 33 25.48709705787514 27.321405888158218 26.116610156242366 21.074886897724273 34 22.74650433355156 27.01678701595646 29.335994371757163 20.900714278734817 35 22.74772574041684 29.02819984170567 27.45918058256222 20.76489383531527 36 24.31405790445667 29.661621442041803 25.465844578419205 20.55847607508232 37 23.727294046374375 29.299596447171712 25.80588424971419 21.167225256739723 38 22.832735658240587 29.405858844451394 25.51885363637252 22.2425518609355 39 23.71630138458682 26.6178755337548 26.750276037951554 22.915547043706823 40 25.161714268963564 26.493292033495862 26.996023099246635 21.34897059829394 41 21.95405555935549 26.84359152245923 25.967598518677754 25.23475439950753 42 23.284167635649446 26.758337323262428 26.791071027252027 23.1664240138361 43 23.068222901867287 26.722427961423083 26.97819055901349 23.231158577696135 44 22.75676415121994 25.220586079870237 27.496311351266844 24.52633841764298 45 22.688853929510167 26.037462991371985 26.19673444660498 25.076948632512874 46 22.291652416919906 28.011012204297398 26.33597482924732 23.361360549535377 47 21.89518374844881 26.769574266423035 28.397953899219274 22.937288085908875 48 21.26420496184325 27.68465228989359 27.676835285955775 23.374307462307385 49 23.204043345286834 26.289072805620428 28.408457998260715 22.098425850832022 50 21.454988714200564 26.998221631604146 28.40748087276849 23.1393087814268 51 21.218768626454697 26.524315767874068 26.961823707018695 25.295091898652544 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 106.0 1 436.0 2 766.0 3 1685.5 4 2605.0 5 1901.5 6 1198.0 7 1172.0 8 1146.0 9 1066.5 10 987.0 11 1009.0 12 1031.0 13 991.0 14 951.0 15 974.5 16 998.0 17 965.0 18 932.0 19 1018.0 20 1104.0 21 1193.5 22 1283.0 23 1613.5 24 1944.0 25 2242.5 26 3064.5 27 3588.0 28 4183.0 29 4778.0 30 5517.5 31 6257.0 32 7738.5 33 9220.0 34 10721.5 35 12223.0 36 12866.5 37 13510.0 38 14999.0 39 16488.0 40 17851.0 41 19214.0 42 20208.0 43 21202.0 44 23787.0 45 26372.0 46 29689.0 47 33006.0 48 33113.5 49 33221.0 50 33201.5 51 33182.0 52 31375.0 53 29568.0 54 27467.5 55 25367.0 56 24825.5 57 24284.0 58 22466.5 59 20649.0 60 18962.0 61 17275.0 62 15390.0 63 13505.0 64 11717.0 65 9929.0 66 8385.0 67 6841.0 68 5796.0 69 4751.0 70 3811.5 71 2872.0 72 2499.0 73 2126.0 74 1631.5 75 856.0 76 575.0 77 444.5 78 314.0 79 243.5 80 173.0 81 118.0 82 63.0 83 46.0 84 29.0 85 23.5 86 18.0 87 16.5 88 15.0 89 14.5 90 14.0 91 9.0 92 4.0 93 2.5 94 1.0 95 0.5 96 0.0 97 0.5 98 1.0 99 0.5 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 409364.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.59076101763154 #Duplication Level Percentage of deduplicated Percentage of total 1 74.46272867527749 25.757224523272633 2 8.505179151986786 5.884012389170341 3 3.4715537948908115 3.6025106303675987 4 2.146640421008029 2.970157031755068 5 1.4283912331059607 2.4704569892024164 6 1.0608747255495998 2.2017878460679 7 0.8034938546626168 1.9455424734039106 8 0.6515799331629918 1.8030916601540308 9 0.5679622187228647 1.7681620837397751 >10 6.801173725832029 47.67521779556224 >50 0.0777918960428393 1.7718227500881572 >100 0.01980157353817728 1.3713707492743628 >500 0.0021215971648047083 0.4772644188073995 >1k 7.071990549349028E-4 0.30137865913414463 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1232 0.30095465160590573 No Hit GCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTCTGC 774 0.18907378274591805 No Hit GAATCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTTC 669 0.1634242385749602 No Hit GAATGATACGGCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGC 508 0.12409493751282477 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCG 484 0.11823218455946298 No Hit GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT 459 0.11212515023304444 No Hit GGGGTTGGGGATTTAGCTCAGTGGTAGAGCGCTTGCCTAGCAAGCGCAAGG 411 0.10039964432632083 No Hit GAATGCTGTCTCTTATACACATCTGACGCGGCGAAAATCGTATGCCGTCTT 410 0.10015536295326409 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.09526973549212925 0.0 2 0.0 0.0 0.0 0.5234949824605974 0.0 3 0.0 0.0 0.0 0.6918048484966924 0.0 4 0.0 0.0 0.0 0.9338876891959235 0.0 5 0.0 0.0 0.0 1.610302811190041 0.0 6 0.0 0.0 0.0 2.08420867492012 0.0 7 0.0 0.0 0.0 2.394446018702182 0.0 8 0.0 0.0 0.0 2.9150096246860984 0.0 9 0.0 0.0 0.0 3.0987092172247683 0.0 10 0.0 0.0 0.0 3.7084355243743956 0.0 11 0.0 0.0 0.0 4.37385798458096 0.0 12 0.0 0.0 0.0 5.070792741911844 0.0 13 0.0 0.0 0.0 5.4281763906938565 0.0 14 0.0 0.0 0.0 5.638502652895712 0.0 15 0.0 0.0 0.0 5.869837113180446 0.0 16 0.0 0.0 0.0 6.268748595382105 0.0 17 0.0 0.0 0.0 6.715294945329829 0.0 18 0.0 0.0 0.0 7.234148581702348 0.0 19 0.0 0.0 0.0 7.528996198981835 0.0 20 0.0 0.0 0.0 7.862684554577345 0.0 21 0.0 0.0 0.0 8.24913768675311 0.0 22 0.0 0.0 0.0 8.62313246890298 0.0 23 0.0 0.0 0.0 9.011539852063201 0.0 24 0.0 0.0 0.0 9.31444875465356 0.0 25 0.0 0.0 0.0 9.591463831699905 0.0 26 0.0 0.0 0.0 9.866524657761797 0.0 27 0.0 0.0 0.0 10.185311849600843 0.0 28 0.0 0.0 0.0 10.459884112916622 0.0 29 0.0 0.0 0.0 10.785755464574315 0.0 30 0.0 0.0 0.0 11.209339365454705 0.0 31 0.0 0.0 0.0 11.534477872993229 0.0 32 0.0 0.0 0.0 11.831035459884113 0.0 33 0.0 0.0 0.0 12.152998309572899 0.0 34 0.0 0.0 0.0 12.504030642655437 0.0 35 0.0 0.0 0.0 12.89292658856177 0.0 36 0.0 0.0 0.0 13.202919650970774 0.0 37 0.0 0.0 0.0 13.543203603638815 0.0 38 0.0 0.0 0.0 13.939672272109908 0.0 39 0.0 0.0 0.0 14.542558700813945 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TCAAGCG 30 2.1620635E-6 45.000004 17 CGGGTAC 35 1.2093733E-7 45.0 6 TCCGCGC 20 7.027959E-4 45.0 41 ACGCATT 20 7.027959E-4 45.0 43 ATTACTC 20 7.027959E-4 45.0 28 TAAGTCG 20 7.027959E-4 45.0 35 TTCCGCG 20 7.027959E-4 45.0 40 ACGGATA 20 7.027959E-4 45.0 15 CCGAATT 20 7.027959E-4 45.0 23 CGGTCTA 25 3.886391E-5 45.0 31 TCCCCCG 20 7.027959E-4 45.0 38 ATCGGAA 20 7.027959E-4 45.0 18 TACGAAT 65 0.0 41.53846 12 CGTTTTT 710 0.0 41.514084 1 GCTACGA 60 3.6379788E-12 41.250004 10 CGTTTTA 60 3.6379788E-12 41.250004 1 ACGTCAT 40 3.4509503E-7 39.375 35 CGCATGG 35 6.238437E-6 38.571426 2 TAGGCTC 35 6.238437E-6 38.571426 41 CGATGCA 35 6.238437E-6 38.571426 10 >>END_MODULE