##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936693.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	181036
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.035120086612608	31.0	31.0	33.0	28.0	34.0
2	31.089534678185554	31.0	31.0	34.0	28.0	34.0
3	30.746729932168186	31.0	30.0	34.0	27.0	34.0
4	34.71027309485406	35.0	35.0	37.0	32.0	37.0
5	35.039555668485825	35.0	35.0	37.0	33.0	37.0
6	35.063821560352636	35.0	35.0	37.0	32.0	37.0
7	31.177445369981662	35.0	35.0	37.0	0.0	37.0
8	33.172595505866234	36.0	35.0	37.0	17.0	37.0
9	36.34232417861641	39.0	35.0	39.0	32.0	39.0
10	36.13134956583221	37.0	35.0	39.0	32.0	39.0
11	36.3651594158068	38.0	35.0	39.0	32.0	39.0
12	36.23910161514837	38.0	35.0	39.0	32.0	39.0
13	35.96511743520626	38.0	35.0	39.0	31.0	39.0
14	37.03200468415122	39.0	35.0	40.0	31.0	41.0
15	37.35865242272255	39.0	35.0	41.0	32.0	41.0
16	37.30713228308182	39.0	35.0	40.0	32.0	41.0
17	37.24735411741311	39.0	35.0	40.0	32.0	41.0
18	37.10248790295853	39.0	35.0	40.0	32.0	41.0
19	36.819847986035924	38.0	35.0	40.0	32.0	41.0
20	36.68529463753066	38.0	35.0	40.0	32.0	41.0
21	36.65828343533883	38.0	35.0	40.0	32.0	41.0
22	36.65390861486113	38.0	35.0	40.0	31.0	41.0
23	36.723121368125675	38.0	35.0	40.0	32.0	41.0
24	36.68316798868733	38.0	35.0	40.0	32.0	41.0
25	36.28091650279502	38.0	35.0	40.0	31.0	41.0
26	36.27681234671557	38.0	35.0	40.0	31.0	41.0
27	36.26622881636801	38.0	35.0	40.0	31.0	41.0
28	36.04354382553746	38.0	35.0	40.0	30.0	41.0
29	35.89516449766897	38.0	35.0	40.0	30.0	41.0
30	35.64973264985969	38.0	35.0	40.0	29.0	41.0
31	35.4744028811949	38.0	34.0	40.0	28.0	41.0
32	35.2516350339159	38.0	34.0	40.0	27.0	41.0
33	34.88424401776442	38.0	34.0	40.0	25.0	41.0
34	34.632829934377696	38.0	34.0	40.0	23.0	41.0
35	34.514179500209906	38.0	34.0	40.0	23.0	41.0
36	34.34814622506021	37.0	34.0	40.0	22.0	41.0
37	34.16658565147264	37.0	33.0	40.0	21.0	41.0
38	34.0128648445613	37.0	33.0	40.0	20.0	41.0
39	33.99901677014516	37.0	33.0	40.0	20.0	41.0
40	33.85547625886564	37.0	33.0	40.0	20.0	41.0
41	33.650782164873284	37.0	33.0	40.0	18.0	41.0
42	33.64003844539208	37.0	33.0	40.0	18.0	41.0
43	33.443154952606115	36.0	33.0	40.0	18.0	41.0
44	33.242045780949645	36.0	32.0	40.0	17.0	41.0
45	33.20402019487837	36.0	32.0	40.0	18.0	41.0
46	33.05961245277182	36.0	32.0	40.0	18.0	41.0
47	33.04485848118606	36.0	32.0	40.0	18.0	41.0
48	32.99536556265052	35.0	32.0	40.0	18.0	41.0
49	32.72193928279458	35.0	31.0	40.0	15.0	41.0
50	32.51906803066793	35.0	31.0	40.0	13.0	41.0
51	30.8908504385868	35.0	28.0	38.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	11.0
9	20.0
10	11.0
11	5.0
12	8.0
13	2.0
14	7.0
15	13.0
16	23.0
17	59.0
18	124.0
19	281.0
20	498.0
21	833.0
22	1175.0
23	1655.0
24	2477.0
25	3270.0
26	4112.0
27	4588.0
28	4506.0
29	4596.0
30	5000.0
31	5919.0
32	7400.0
33	10355.0
34	16986.0
35	17385.0
36	15935.0
37	22971.0
38	28938.0
39	21871.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.60281932875229	19.297266841954087	22.418745442895336	19.681168386398284
2	36.059678737930575	23.147882189177842	24.442099913829292	16.350339159062287
3	29.788550343578073	22.19006164519764	33.00669480103405	15.014693210190238
4	27.987803530789456	23.17052961841844	31.61415409089905	17.22751275989306
5	22.59992487682008	27.691729821692924	32.650964449059856	17.057380852427144
6	23.399765792439073	32.268167657261536	30.66517156808591	13.666894982213481
7	65.56265052254801	16.687841092379415	14.437459952716585	3.3120484323559953
8	76.74109016991095	4.557657040588612	14.412050641861288	4.289202147639144
9	71.17810822156919	6.06840628383305	16.986676683090657	5.7668088115071034
10	40.140635011820855	24.426081000463995	22.948474336596036	12.484809651119114
11	31.66662984157847	24.82434432930467	27.658587242316447	15.850438586800417
12	30.21388011224287	20.397048100930203	33.0381802514417	16.35089153538523
13	23.703572770056784	26.64829094765682	32.6515168253828	16.9966194569036
14	17.12421838750304	32.01296979606266	32.76751585320047	18.09529596323383
15	16.80107823858238	22.51817318102477	43.156609735080316	17.524138845312535
16	17.47000596566429	23.428489361232018	40.569279038423296	18.532225634680394
17	18.164343003601495	21.63879007490223	32.53165116330454	27.665215758191742
18	23.57486908681146	21.816655250889326	35.020106498155066	19.588369164144147
19	24.738725999248768	23.581497602686756	31.42027000154665	20.25950639651782
20	23.909609138513886	22.62091517709185	31.521354868644913	21.948120815749352
21	22.687200335844803	26.043438874036102	32.198568240570935	19.07079254954816
22	19.556883713736497	24.94420999138293	32.03396009633443	23.464946198546148
23	18.27758014980446	25.626394750215425	32.18365407985152	23.912371020128592
24	20.114231423584258	22.583353587131842	35.268123467155704	22.034291522128196
25	18.617843964736295	24.369186239201042	33.56017587662122	23.452793919441437
26	17.28661702644778	27.03771625533043	32.2770056784286	23.39866103979319
27	18.626681985903357	26.189266223292606	32.85534368854813	22.328708102255902
28	17.661128173401973	25.503214830199518	37.20585960803376	19.629797388364743
29	18.683024370843366	23.160586844605493	38.07032855343688	20.086060231114253
30	21.31564992598157	24.555337060032258	34.58704346096909	19.54196955301708
31	22.614286661216553	27.2139243023487	31.087739455135992	19.08404958129875
32	23.144015554917257	28.91192911907024	29.494133763450364	18.449921562562142
33	23.912371020128592	25.980467973220794	30.20669922004463	19.90046178660598
34	21.60509511920281	25.741841401710158	31.151262732274244	21.501800746812787
35	18.9188890607393	25.677213371926026	31.69314390507965	23.710753662255023
36	24.57246072604344	25.182836562893566	30.58286749596765	19.66183521509534
37	20.33242007114607	28.51918955345898	31.643430036014937	19.504960339380013
38	20.705826465454386	28.956119224905542	28.067898097615945	22.270156212024126
39	23.558850173446167	25.113237146202966	29.433372367926818	21.89454031242405
40	24.06040787467686	24.125588280783933	30.481230252546453	21.332773591992755
41	19.95238516096246	23.486488875140857	29.82058817030867	26.740537793588015
42	23.33016637574847	23.980865684173313	28.91579575333083	23.773172186747388
43	22.5573918999536	24.38078614198281	29.849311739101616	23.212510218961974
44	20.54121832121788	26.98965951523454	29.474800592147414	22.994321571400164
45	20.205925893192514	27.137696369782805	27.16144855166928	25.494929185355396
46	22.67670518570892	26.90127930356393	28.846748712963166	21.57526679776398
47	20.584303674407302	24.356481583773395	31.68320113126671	23.376013610552597
48	21.592942840098104	22.93742681013721	31.34569919794958	24.12393115181511
49	21.67248503060165	23.03574979562076	32.19193972469564	23.09982544908195
50	19.179610685167592	23.76599129454915	33.39114872180119	23.66324929848207
51	20.146821626637795	24.599527165867563	30.579553238030005	24.674097969464636
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	126.0
1	238.0
2	350.0
3	2741.0
4	5132.0
5	3519.5
6	1907.0
7	1809.5
8	1712.0
9	1620.0
10	1528.0
11	1496.0
12	1464.0
13	1341.0
14	1218.0
15	1123.0
16	1028.0
17	991.5
18	955.0
19	867.0
20	779.0
21	789.5
22	800.0
23	891.5
24	983.0
25	1089.5
26	1167.5
27	1139.0
28	1384.0
29	1629.0
30	1922.5
31	2216.0
32	2488.5
33	2761.0
34	3329.0
35	3897.0
36	4157.0
37	4417.0
38	5356.5
39	6296.0
40	7515.5
41	8735.0
42	9722.5
43	10710.0
44	12404.0
45	14098.0
46	15348.5
47	16599.0
48	16877.5
49	17156.0
50	16111.0
51	15066.0
52	13419.5
53	11773.0
54	10600.5
55	9428.0
56	8529.0
57	7630.0
58	7353.0
59	7076.0
60	6805.0
61	6534.0
62	5690.5
63	4847.0
64	4267.0
65	3687.0
66	3064.0
67	2441.0
68	2019.5
69	1598.0
70	1283.0
71	968.0
72	721.5
73	475.0
74	350.5
75	230.0
76	234.0
77	172.5
78	111.0
79	79.0
80	47.0
81	35.5
82	24.0
83	18.5
84	13.0
85	13.5
86	14.0
87	10.0
88	6.0
89	4.5
90	3.0
91	2.5
92	2.0
93	2.0
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	181036.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.2021476391436
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.16485839086938	31.42634614109901
2	7.628575454417359	5.981130824808326
3	2.891362547555305	3.4004286440266025
4	1.5654501902212201	2.454760379151108
5	1.0455121882485559	2.0493161581121986
6	0.7665210652388333	1.8029563180803818
7	0.5368465548823446	1.4731876532844297
8	0.42412286881781036	1.3301221856426346
9	0.30576299845004934	1.078790958704346
>10	4.441313230942652	40.611259638966835
>50	0.20712977314358177	4.909520758302216
>100	0.021135691137100183	1.805165823372147
>500	0.0	0.0
>1k	0.0014090460758066788	1.677014516449767
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3036	1.677014516449767	No Hit
GCTGTCTCTTATACACATCTGACGCGTAAGTCGTCGTATGCCGTCTTCTGC	474	0.2618263770741731	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTAAGTCGTCGTATGCCGTCTTC	380	0.20990300271769152	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTAAGTCGTCGTATGCCG	339	0.18725557347709848	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTAAGTCGTCGTATGC	299	0.16516052055944674	No Hit
CTGTCTCTTATACACATCTGACGCGTAAGTCGTCGTATGCCGTCTTCTGCT	259	0.143065467641795	No Hit
CCTGTCTCTTATACACATCTGACGCGTAAGTCGTCGTATGCCGTCTTCTGC	249	0.13754170441238206	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGTAAGTCGTCGTATGCCGTCTT	230	0.1270465542764975	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	202	0.11158001723414128	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.523763229412934E-4	0.0	0.0	0.18228418657062684	0.0
2	5.523763229412934E-4	0.0	0.0	0.8882211272895999	0.0
3	5.523763229412934E-4	0.0	0.0	1.1235334408625908	0.0
4	5.523763229412934E-4	0.0	0.0	1.4499878477208954	0.0
5	5.523763229412934E-4	0.0	0.0	2.38295145716874	0.0
6	5.523763229412934E-4	0.0	0.0	3.0330983892706422	0.0
7	5.523763229412934E-4	0.0	0.0	3.5352084668242783	0.0
8	5.523763229412934E-4	0.0	0.0	4.393601272675048	0.0
9	5.523763229412934E-4	0.0	0.0	4.700170131907466	0.0
10	5.523763229412934E-4	0.0	0.0	5.620429085927661	0.0
11	5.523763229412934E-4	0.0	0.0	6.705296184184361	0.0
12	5.523763229412934E-4	0.0	0.0	7.879648246757551	0.0
13	5.523763229412934E-4	0.0	0.0	8.610442122008882	0.0
14	5.523763229412934E-4	0.0	0.0	9.010362579818379	0.0
15	5.523763229412934E-4	0.0	0.0	9.319693320665502	0.0
16	5.523763229412934E-4	0.0	0.0	9.956583221016814	0.0
17	5.523763229412934E-4	0.0	0.0	10.695110364789324	0.0
18	5.523763229412934E-4	0.0	0.0	11.686073488146004	0.0
19	5.523763229412934E-4	0.0	0.0	12.092622461830796	0.0
20	5.523763229412934E-4	0.0	0.0	12.547780551934421	0.0
21	5.523763229412934E-4	0.0	0.0	13.060385779623942	0.0
22	5.523763229412934E-4	0.0	0.0	13.598952694491704	0.0
23	5.523763229412934E-4	0.0	0.0	14.104377029982986	0.0
24	5.523763229412934E-4	0.0	0.0	14.516449766897193	0.0
25	5.523763229412934E-4	0.0	0.0	14.841799421109613	0.0
26	5.523763229412934E-4	0.0	0.0	15.174329967520272	0.0
27	5.523763229412934E-4	0.0	0.0	15.498574869086811	0.0
28	5.523763229412934E-4	0.0	0.0	15.847676705185709	0.0
29	5.523763229412934E-4	0.0	0.0	16.19346428334696	0.0
30	5.523763229412934E-4	0.0	0.0	16.618794052011754	0.0
31	5.523763229412934E-4	0.0	0.0	16.98778143573654	0.0
32	5.523763229412934E-4	0.0	0.0	17.30871207936543	0.0
33	5.523763229412934E-4	0.0	0.0	17.692061247486688	0.0
34	5.523763229412934E-4	0.0	0.0	18.07817229722265	0.0
35	5.523763229412934E-4	0.0	0.0	18.527254247773925	0.0
36	5.523763229412934E-4	0.0	0.0	18.912260544864004	0.0
37	5.523763229412934E-4	0.0	0.0	19.2679908968382	0.0
38	5.523763229412934E-4	0.0	0.0	19.63753065688592	0.0
39	5.523763229412934E-4	0.0	0.0	20.01646081442365	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGGGCC	30	2.1553406E-6	45.000004	5
CGGGCAT	35	1.2043529E-7	45.000004	6
GGAACGG	30	2.1553406E-6	45.000004	2
GAACCCC	30	2.1553406E-6	45.000004	18
CTTCGAG	20	7.017528E-4	45.0	30
CGGGTAA	25	3.8777496E-5	45.0	6
AGATCCA	20	7.017528E-4	45.0	30
TCACCGG	20	7.017528E-4	45.0	2
TGCTTAG	25	3.8777496E-5	45.0	1
ACTGATC	20	7.017528E-4	45.0	39
CTATCTC	20	7.017528E-4	45.0	5
TGAAGCG	20	7.017528E-4	45.0	1
TATGGGT	20	7.017528E-4	45.0	4
AGCTTCG	20	7.017528E-4	45.0	28
TAGCGGG	20	7.017528E-4	45.0	3
GACCGAC	20	7.017528E-4	45.0	9
GTGATTC	20	7.017528E-4	45.0	8
CGATTGG	20	7.017528E-4	45.0	2
TGACGTG	20	7.017528E-4	45.0	20
TACGGGC	20	7.017528E-4	45.0	4
>>END_MODULE