##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936691.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	590117
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.14604561468319	31.0	31.0	33.0	28.0	34.0
2	31.18872698125965	31.0	31.0	34.0	28.0	34.0
3	30.99398085464408	31.0	31.0	34.0	28.0	34.0
4	34.97681985097871	35.0	35.0	37.0	32.0	37.0
5	35.189221798389134	35.0	35.0	37.0	33.0	37.0
6	35.162084806911174	36.0	35.0	37.0	32.0	37.0
7	31.043393089844894	35.0	35.0	37.0	0.0	37.0
8	33.08626255471373	36.0	35.0	37.0	17.0	37.0
9	36.34540946964754	39.0	35.0	39.0	32.0	39.0
10	36.0967825024529	37.0	35.0	39.0	32.0	39.0
11	36.38082617514832	38.0	35.0	39.0	32.0	39.0
12	36.42409556071084	38.0	35.0	39.0	32.0	39.0
13	36.3552210832767	38.0	35.0	39.0	32.0	39.0
14	37.31417498563844	39.0	36.0	40.0	32.0	41.0
15	37.50392718732048	39.0	36.0	41.0	32.0	41.0
16	37.45749063321341	39.0	36.0	41.0	32.0	41.0
17	37.378240247273	39.0	36.0	40.0	32.0	41.0
18	37.30818125897068	39.0	36.0	40.0	32.0	41.0
19	37.12834065109122	39.0	36.0	40.0	32.0	41.0
20	37.14984994501091	39.0	35.0	40.0	32.0	41.0
21	37.108639473189214	39.0	35.0	40.0	32.0	41.0
22	37.25059267907889	39.0	35.0	40.0	32.0	41.0
23	37.31951460473093	39.0	35.0	40.0	32.0	41.0
24	37.26063136632227	39.0	35.0	41.0	32.0	41.0
25	36.91593531452237	39.0	35.0	40.0	31.0	41.0
26	36.936597318836775	39.0	35.0	40.0	31.0	41.0
27	36.95450902109243	39.0	35.0	40.0	31.0	41.0
28	36.929949484593735	39.0	35.0	40.0	31.0	41.0
29	36.93045616377769	39.0	35.0	40.0	31.0	41.0
30	36.70067461198372	39.0	35.0	40.0	31.0	41.0
31	36.66876907460724	39.0	35.0	40.0	31.0	41.0
32	36.52802071453627	39.0	35.0	40.0	30.0	41.0
33	36.42678655249722	39.0	35.0	40.0	30.0	41.0
34	36.33130548687802	39.0	35.0	40.0	30.0	41.0
35	36.308191426445944	39.0	35.0	40.0	30.0	41.0
36	36.24637317684459	39.0	35.0	40.0	30.0	41.0
37	36.16020890772508	39.0	35.0	40.0	30.0	41.0
38	35.99946790212788	38.0	35.0	40.0	29.0	41.0
39	35.97424917431628	38.0	35.0	40.0	29.0	41.0
40	35.85636577153344	38.0	35.0	40.0	29.0	41.0
41	35.65416688554981	38.0	35.0	40.0	28.0	41.0
42	35.66709144118878	38.0	35.0	40.0	28.0	41.0
43	35.60790487310143	38.0	35.0	40.0	28.0	41.0
44	35.38139555376307	38.0	34.0	40.0	27.0	41.0
45	35.29024244344765	38.0	34.0	40.0	27.0	41.0
46	35.15970053396191	38.0	34.0	40.0	26.0	41.0
47	35.0737294468724	38.0	34.0	40.0	26.0	41.0
48	34.96766912324166	38.0	34.0	40.0	26.0	41.0
49	34.87741244532864	37.0	34.0	40.0	26.0	41.0
50	34.698598752450785	37.0	34.0	40.0	25.0	41.0
51	33.005832741642756	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	18.0
9	50.0
10	57.0
11	46.0
12	29.0
13	31.0
14	39.0
15	39.0
16	97.0
17	168.0
18	322.0
19	591.0
20	949.0
21	1386.0
22	1944.0
23	2925.0
24	4065.0
25	5482.0
26	6846.0
27	8116.0
28	9244.0
29	11007.0
30	13728.0
31	17665.0
32	22495.0
33	30676.0
34	46055.0
35	50104.0
36	59307.0
37	87579.0
38	116152.0
39	92899.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.7306212157928	19.95417857814637	23.163203229190145	12.151996976870688
2	32.80739243234816	24.489719835219116	24.71238754348714	17.990500188945582
3	33.1603732819085	23.6744577770171	26.75367766053173	16.411491280542673
4	29.73122279141255	25.609836693401476	25.541206235373664	19.11773427981231
5	25.895881664144564	28.946293701079618	24.978436479545582	20.179388155230235
6	26.439502674893284	34.69396746746832	25.14535253178607	13.721177325852333
7	69.62720951946817	17.988805609735017	8.850448300930154	3.5335365698666537
8	80.97394245547916	6.116753118449393	8.61269883768812	4.29660558838332
9	74.2908609648595	7.8694563959350425	10.828530613420728	7.0111520257847175
10	40.25455968901082	27.422866990783184	18.05930010489445	14.26327321531154
11	31.52984916550447	26.38154806589202	23.98964951018188	18.098953258421634
12	28.74667227007526	23.950674188338922	28.33065307388196	18.97200046770386
13	26.455092803630464	25.119086554022342	29.297579971429393	19.1282406709178
14	21.52522296425963	27.948017088136762	29.39552664979995	21.131233297803657
15	20.07567990754376	26.973972957904955	33.38693852236082	19.563408612190464
16	24.340427406768487	26.66640683118771	29.18099292174264	19.81217284030116
17	24.178425634238636	25.8658876121176	27.41100493630924	22.544681817334528
18	25.352769027159024	24.494973030771863	29.43501034540608	20.717247596663036
19	24.684426986512843	27.430831513072828	26.471021848209762	21.413719652204563
20	26.896700145903267	26.61726403408138	27.66891989215698	18.817115927858374
21	25.00131329888819	27.404734993230157	29.035259109634193	18.558692598247468
22	24.75068503364587	23.530418544119218	29.153032364768343	22.56586405746657
23	22.69346587202199	26.96922813611538	28.94663261692173	21.390673374940903
24	23.452976274196473	25.332603534553318	28.917655312421097	22.296764878829116
25	23.37180593001049	27.359998102071287	27.280352879174806	21.987843088743418
26	22.307440727855663	26.448992318472435	27.60181455541867	23.641752398253228
27	22.199326574221722	27.144278168566572	28.40351997993618	22.252875277275525
28	19.70033061240398	28.117475009193093	29.34841734774629	22.833777030656634
29	22.87648042676283	26.756388987268625	28.568402537123994	21.798728048844552
30	23.46280483361774	25.529174722978667	29.356381870035943	21.651638573367656
31	24.482433144613694	27.067513730328052	27.067852646170167	21.382200478888088
32	25.481895963003947	26.527620793842576	27.519119089943178	20.471364153210295
33	24.648501907248903	25.52510773287331	27.67688441444663	22.149505945431162
34	22.41436867604221	26.222257620099064	29.569559934724808	21.79381376913392
35	22.822762265787972	26.259368904810405	27.96411559063711	22.953753238764516
36	23.857641789678997	28.51282033901752	26.30275013260082	21.326787738702663
37	23.587525863515204	28.248127066327527	26.839762284428343	21.32458478572893
38	21.83160288552948	30.063699232525074	26.765709172926726	21.338988709018718
39	23.218615969375563	25.242282462630293	27.870574818214017	23.668526749780128
40	25.461899928319298	24.506157253561582	28.26676743764372	21.765175380475394
41	21.88057622471476	26.292074283574273	27.567414597444234	24.25993489426673
42	23.002387662107683	25.78776751051063	27.656041090156698	23.55380373722499
43	22.983747290791488	26.41916772436652	27.29831541880678	23.29876956603521
44	22.343704722961718	26.003148528173227	27.7817788675805	23.87136788128456
45	22.926978887237613	24.76373329356721	27.14631166361925	25.16297615557593
46	22.772602721155298	26.485256313578493	27.182745116646363	23.55939584861985
47	21.646724293657023	26.966686267299533	29.325032154640517	22.061557284402923
48	21.823129989476662	26.880771101323976	28.73955503739089	22.556543871808472
49	23.184893843085355	24.91285626409678	29.64818841009495	22.254061482722918
50	21.21664009001605	26.59964041029152	29.186585033137497	22.99713446655494
51	21.506074219180267	25.212627326445435	27.84396992460817	25.43732852976613
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	187.0
1	580.5
2	974.0
3	2185.5
4	3397.0
5	2415.0
6	1433.0
7	1400.0
8	1367.0
9	1318.0
10	1269.0
11	1335.5
12	1402.0
13	1348.5
14	1295.0
15	1341.0
16	1387.0
17	1425.0
18	1463.0
19	1569.5
20	1676.0
21	1824.5
22	1973.0
23	2247.5
24	2522.0
25	3204.5
26	4775.0
27	5663.0
28	6567.0
29	7471.0
30	8454.0
31	9437.0
32	10998.5
33	12560.0
34	14715.5
35	16871.0
36	18472.5
37	20074.0
38	21440.0
39	22806.0
40	25251.0
41	27696.0
42	30442.0
43	33188.0
44	35448.5
45	37709.0
46	45116.0
47	52523.0
48	52562.0
49	52601.0
50	52097.5
51	51594.0
52	46701.5
53	41809.0
54	38537.5
55	35266.0
56	33419.5
57	31573.0
58	29361.0
59	27149.0
60	25083.5
61	23018.0
62	20824.5
63	18631.0
64	15904.5
65	13178.0
66	11386.0
67	9594.0
68	8041.0
69	6488.0
70	5369.0
71	4250.0
72	3297.0
73	2344.0
74	1725.5
75	872.0
76	637.0
77	447.0
78	257.0
79	196.5
80	136.0
81	126.5
82	117.0
83	91.0
84	65.0
85	52.0
86	39.0
87	26.0
88	13.0
89	11.0
90	9.0
91	5.5
92	2.0
93	3.0
94	4.0
95	3.0
96	2.0
97	2.5
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	590117.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	32.41709986401658
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.12032196981949	24.027658792487
2	8.881995491702359	5.758570696925207
3	3.7059028880784677	3.604038720275614
4	2.214116055004391	2.8710088506239906
5	1.5103563318430064	2.448068601980225
6	1.0239233894850783	1.9915576062024074
7	0.7629908969082292	1.731376647028675
8	0.5896889608429884	1.5292804745884252
9	0.4615277906033294	1.3465253230206353
>10	6.506666513065544	47.67131270460781
>50	0.1877587785041337	3.705424923239834
>100	0.031120239531055433	1.814040724966717
>500	0.001037341317701848	0.19957978872328233
>1k	0.0025933532942546194	1.301556145330184
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCG	1716	0.29078979253266724	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGC	1701	0.28824792371682223	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1650	0.27960556974294926	No Hit
GAATCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTC	1413	0.23944404245259837	No Hit
GCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCTGC	1261	0.21368643845203578	No Hit
CCTGTCTCTTATACACATCTGACGCCGATTGGATCGTATGCCGTCTTCTGC	636	0.10777523779182771	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.694579210563329E-4	0.0	0.0	0.09726884668633508	0.0
2	1.694579210563329E-4	0.0	0.0	0.6093706841185731	0.0
3	1.694579210563329E-4	0.0	0.0	0.770186251201033	0.0
4	1.694579210563329E-4	0.0	0.0	1.0484361575755317	0.0
5	1.694579210563329E-4	0.0	0.0	1.917077460910294	0.0
6	1.694579210563329E-4	0.0	0.0	2.403082778499857	0.0
7	1.694579210563329E-4	0.0	0.0	2.8116458261666755	0.0
8	1.694579210563329E-4	0.0	0.0	3.4735484658127116	0.0
9	1.694579210563329E-4	0.0	0.0	3.703163948844043	0.0
10	1.694579210563329E-4	0.0	0.0	4.561298861073313	0.0
11	1.694579210563329E-4	0.0	0.0	5.3265708325637116	0.0
12	1.694579210563329E-4	0.0	0.0	6.237746074083614	0.0
13	1.694579210563329E-4	0.0	0.0	6.533450146326915	0.0
14	1.694579210563329E-4	0.0	0.0	6.680031248040643	0.0
15	1.694579210563329E-4	0.0	0.0	6.929642765756621	0.0
16	1.694579210563329E-4	0.0	0.0	7.377350593187453	0.0
17	1.694579210563329E-4	0.0	0.0	7.876404170698353	0.0
18	1.694579210563329E-4	0.0	0.0	8.480691117185236	0.0
19	1.694579210563329E-4	0.0	0.0	8.836891667245649	0.0
20	1.694579210563329E-4	0.0	0.0	9.192922759385004	0.0
21	1.694579210563329E-4	0.0	0.0	9.628768532341892	0.0
22	1.694579210563329E-4	0.0	0.0	10.061055688956596	0.0
23	1.694579210563329E-4	0.0	0.0	10.499782246571442	0.0
24	1.694579210563329E-4	0.0	0.0	10.83818971492094	0.0
25	1.694579210563329E-4	0.0	0.0	11.173546940691422	0.0
26	1.694579210563329E-4	0.0	0.0	11.483824394145568	0.0
27	1.694579210563329E-4	0.0	0.0	11.806472275836825	0.0
28	1.694579210563329E-4	0.0	0.0	12.127595036238576	0.0
29	1.694579210563329E-4	0.0	0.0	12.473967026877721	0.0
30	1.694579210563329E-4	0.0	0.0	12.900662072097568	0.0
31	1.694579210563329E-4	0.0	0.0	13.264488228605513	0.0
32	1.694579210563329E-4	0.0	0.0	13.603912444481349	0.0
33	1.694579210563329E-4	0.0	0.0	13.961468657910212	0.0
34	1.694579210563329E-4	0.0	0.0	14.324955898576045	0.0
35	1.694579210563329E-4	0.0	0.0	14.736738646742934	0.0
36	1.694579210563329E-4	0.0	0.0	15.094803233934966	0.0
37	1.694579210563329E-4	0.0	0.0	15.451681615679602	0.0
38	1.694579210563329E-4	0.0	0.0	15.864820027214941	0.0
39	1.694579210563329E-4	0.0	0.0	16.427589783043025	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAAACGG	25	3.8884897E-5	45.000004	2
CTACGAA	105	0.0	45.000004	11
TCGTACG	50	2.1827873E-11	45.000004	30
CGATTCG	20	7.0304936E-4	45.0	10
TATGCGA	20	7.0304936E-4	45.0	37
TCCCGAT	20	7.0304936E-4	45.0	45
GCGGACT	20	7.0304936E-4	45.0	24
CCAGTCG	20	7.0304936E-4	45.0	28
CGTTTTA	70	0.0	41.785717	1
CGACGGT	60	3.6379788E-12	41.249996	28
TACGAAT	115	0.0	41.086956	12
GCTACGA	115	0.0	41.086956	10
TCGTTCA	50	1.0804797E-9	40.500004	16
GCCGATA	50	1.0804797E-9	40.500004	9
CGGTCTA	50	1.0804797E-9	40.500004	31
CGTTAGG	45	1.9255822E-8	40.0	2
TACGATG	35	6.2431227E-6	38.57143	1
AACGGGA	175	0.0	38.571426	4
CACAACG	275	0.0	38.454544	12
AGGCGAT	100	0.0	38.250004	7
>>END_MODULE