##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936690.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	463255
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.164973934442155	31.0	31.0	33.0	28.0	34.0
2	31.190627192367057	31.0	31.0	34.0	28.0	34.0
3	30.979866380287316	31.0	31.0	34.0	28.0	34.0
4	34.93527322964674	35.0	35.0	37.0	32.0	37.0
5	35.159050630862055	35.0	35.0	37.0	33.0	37.0
6	35.11989940745378	36.0	35.0	37.0	32.0	37.0
7	30.955775976514015	35.0	35.0	37.0	0.0	37.0
8	32.95514781275971	35.0	35.0	37.0	17.0	37.0
9	36.18330940842517	39.0	35.0	39.0	32.0	39.0
10	36.08407032843682	37.0	35.0	39.0	32.0	39.0
11	36.32205588714639	37.0	35.0	39.0	32.0	39.0
12	36.39377880433023	38.0	35.0	39.0	32.0	39.0
13	36.252182923012164	38.0	35.0	39.0	32.0	39.0
14	37.38018801739862	39.0	36.0	40.0	32.0	41.0
15	37.48411997711843	39.0	36.0	41.0	32.0	41.0
16	37.51436897604991	39.0	36.0	41.0	33.0	41.0
17	37.335486934841505	39.0	36.0	41.0	32.0	41.0
18	37.22758523923109	39.0	36.0	40.0	32.0	41.0
19	37.031105978348855	39.0	35.0	40.0	31.0	41.0
20	36.96781038520901	39.0	35.0	40.0	31.0	41.0
21	37.08109140754012	39.0	35.0	40.0	32.0	41.0
22	37.17300838630992	39.0	35.0	40.0	32.0	41.0
23	37.22707148330833	39.0	35.0	40.0	32.0	41.0
24	37.11324000820282	39.0	35.0	40.0	32.0	41.0
25	36.88304497522962	39.0	35.0	40.0	31.0	41.0
26	36.762884372537805	39.0	35.0	40.0	31.0	41.0
27	36.805295139825795	39.0	35.0	40.0	31.0	41.0
28	36.723627375851315	39.0	35.0	40.0	31.0	41.0
29	36.67709576798955	39.0	35.0	40.0	31.0	41.0
30	36.49306105708519	39.0	35.0	40.0	30.0	41.0
31	36.42503588736225	38.0	35.0	40.0	30.0	41.0
32	36.28372494630387	38.0	35.0	40.0	30.0	41.0
33	36.131735221422325	38.0	35.0	40.0	30.0	41.0
34	36.03826186441593	38.0	35.0	40.0	30.0	41.0
35	35.99967836288869	39.0	35.0	40.0	29.0	41.0
36	35.86156436519843	38.0	35.0	40.0	29.0	41.0
37	35.834192831162106	38.0	35.0	40.0	29.0	41.0
38	35.60265728378539	38.0	35.0	40.0	27.0	41.0
39	35.64262663112109	38.0	35.0	40.0	28.0	41.0
40	35.54117062956687	38.0	35.0	40.0	27.0	41.0
41	35.39176911204412	38.0	34.0	40.0	26.0	41.0
42	35.43066777476768	38.0	34.0	40.0	27.0	41.0
43	35.39105460275658	38.0	34.0	40.0	27.0	41.0
44	35.22935316402413	38.0	34.0	40.0	26.0	41.0
45	35.12762517404022	38.0	34.0	40.0	26.0	41.0
46	35.03617014387324	38.0	34.0	40.0	26.0	41.0
47	34.91657726306246	38.0	34.0	40.0	25.0	41.0
48	34.8413573517825	37.0	34.0	40.0	25.0	41.0
49	34.72415408360406	37.0	34.0	40.0	24.0	41.0
50	34.55301723672707	37.0	34.0	40.0	24.0	41.0
51	32.75310574089864	35.0	31.0	39.0	20.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	15.0
9	28.0
10	52.0
11	35.0
12	27.0
13	17.0
14	25.0
15	45.0
16	70.0
17	121.0
18	262.0
19	531.0
20	861.0
21	1372.0
22	1971.0
23	2594.0
24	3518.0
25	4884.0
26	6093.0
27	6969.0
28	7866.0
29	8969.0
30	10898.0
31	13844.0
32	18122.0
33	24693.0
34	37533.0
35	40381.0
36	45012.0
37	66176.0
38	89047.0
39	71215.0
40	6.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.65500642194903	18.792241853838597	22.751400416617198	14.801351307595171
2	30.871550226117368	28.300827837799915	23.760347972498945	17.06727396358377
3	30.42946109594068	27.651725291686002	26.736030911700897	15.182782700672416
4	28.627645681104358	23.47195389148525	30.51300039934809	17.3874000280623
5	27.38988246214288	28.326083906271926	24.661363611833657	19.62267001975154
6	25.301399876957614	36.801113857378766	24.762387885721687	13.135098379941931
7	65.96993016805	17.519508693915878	13.029109237892737	3.4814519001413906
8	75.92945569934486	9.256025299241239	10.603231481581417	4.2112875198324895
9	70.42665486611045	7.301162426741212	12.030091418333315	10.242091288815015
10	41.19653322684051	25.077117354372863	19.223969520026767	14.502379898759862
11	33.08976697499217	24.161854702053944	24.99660014462877	17.75177817832511
12	31.194266656593022	20.994916406730635	28.494241832252214	19.316575104424132
13	24.853914150953578	23.43655222285782	31.73392623932823	19.975607386860368
14	20.255582778383395	29.173349451166203	27.678060679323487	22.89300709112692
15	18.76331610020399	25.47927167542714	37.076987836073	18.68042438829586
16	21.100689684946737	24.22596626048289	32.91729177235	21.756052282220377
17	21.290002266570248	24.480037992034624	30.65719743985494	23.572762301540187
18	21.736408673408814	26.390217051084175	29.47037808550366	22.402996190003346
19	23.914043021661936	27.340449644364334	27.069756397664353	21.675750936309377
20	26.689836051418762	25.397675146517578	29.79201519681385	18.12047360524981
21	25.32795112842819	27.165168211891938	27.835425413649066	19.671455246030803
22	22.334567354912522	23.982255992919665	28.10244897518645	25.580727676981358
23	23.339845225631674	27.38621277698028	28.67988472871313	20.59405726867492
24	23.803952466783954	24.263310703608166	28.67945300104694	23.253283828560946
25	22.62576766575644	28.515396487895433	26.750277924685108	22.108557921663017
26	21.55184509611337	26.41245102589287	28.66693289872748	23.36877097926628
27	23.743294729684514	26.85713052206668	27.58178540976352	21.817789338485284
28	20.240256446233715	26.454544473346214	31.912445629297036	21.39275345112303
29	24.587322317082386	25.933233316423998	28.244703241195452	21.23474112529816
30	24.951700467345198	25.47495439876526	29.091537058423544	20.481808075465995
31	24.840962320967932	25.575762808820198	27.037160958867148	22.546113911344722
32	26.40619097473314	28.138929962979354	26.57801858587603	18.87686047641148
33	25.216565390551636	26.71660316672243	25.155044198119825	22.911787244606103
34	24.827794627149196	25.938845776084445	27.2176231233338	22.01573647343256
35	24.52321075865344	25.11726802732836	27.129118951765225	23.230402262252973
36	24.550409601623297	28.83012595654661	26.586221411533607	20.03324303029649
37	23.9334707666404	29.05462434296446	27.664677121671648	19.347227768723492
38	23.613776429828064	27.152216381906292	26.77682917615568	22.45717801210996
39	23.936708724136814	26.72718049454404	25.9442423719118	23.391868409407344
40	24.855641061618332	24.226829715815263	30.616615039233253	20.300914183333155
41	20.30760596215907	28.282479411986916	27.383190683316965	24.02672394253705
42	21.718060247595815	26.41590484722237	29.907502347519184	21.95853255766263
43	23.689328771410995	27.019028396887247	26.155357200677816	23.136285631023952
44	23.11556270304692	25.97359985321259	27.630138908376594	23.280698535363893
45	21.956805646997875	24.477447626037495	26.924695901825128	26.6410508251395
46	24.341237547355128	28.063161757563325	25.284994225642464	22.31060646943908
47	22.107910330163733	25.102157559011772	31.29399574748249	21.495936363342004
48	22.200084186894905	25.053588196565606	28.449558018801742	24.296769597737747
49	21.18703521818437	24.86017420211331	31.180667235108096	22.77212334459423
50	20.644353541785843	24.402974603620038	30.012196306569816	24.940475548024306
51	21.169334383870655	24.164013340384884	27.95004910902203	26.71660316672243
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	149.0
1	506.0
2	863.0
3	3170.0
4	5477.0
5	3812.0
6	2147.0
7	2033.5
8	1920.0
9	1833.0
10	1746.0
11	1675.0
12	1604.0
13	1547.5
14	1491.0
15	1564.0
16	1637.0
17	1555.5
18	1474.0
19	1577.0
20	1680.0
21	1711.5
22	1743.0
23	1875.0
24	2007.0
25	2662.5
26	3445.0
27	3572.0
28	4462.5
29	5353.0
30	6411.5
31	7470.0
32	8162.5
33	8855.0
34	10001.5
35	11148.0
36	12512.5
37	13877.0
38	14831.5
39	15786.0
40	17966.0
41	20146.0
42	21648.5
43	23151.0
44	26506.5
45	29862.0
46	41916.5
47	53971.0
48	45446.5
49	36922.0
50	35424.5
51	33927.0
52	31178.5
53	28430.0
54	26565.0
55	24700.0
56	24650.0
57	24600.0
58	23653.5
59	22707.0
60	22422.5
61	22138.0
62	20116.0
63	18094.0
64	15121.0
65	12148.0
66	9505.0
67	6862.0
68	5627.0
69	4392.0
70	3654.0
71	2916.0
72	2490.0
73	2064.0
74	1682.5
75	1090.5
76	880.0
77	635.5
78	391.0
79	272.0
80	153.0
81	114.5
82	76.0
83	62.0
84	48.0
85	39.0
86	30.0
87	21.5
88	13.0
89	10.0
90	7.0
91	5.5
92	4.0
93	4.5
94	5.0
95	2.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	463255.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.237934702521645
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.05522484740136	22.39277053316281
2	8.505960570380914	5.144053606188037
3	3.54983864791862	3.2201936772075292
4	2.0505820489709907	2.4802146439579142
5	1.5388819699764282	2.326630626151756
6	1.080685997612802	1.9606627577847227
7	0.824137244636261	1.7444145732459158
8	0.6569387829229617	1.5891577617262849
9	0.5623623696225013	1.5304208950620484
>10	6.921424203416544	45.941068291180684
>50	0.20960916034422736	3.862809238392436
>100	0.035054091661662595	2.1296610265987304
>500	0.004292337754489298	0.8075190058195085
>1k	0.002861558502992865	1.4091022780359705
>5k	0.002146168877244649	3.4613210854856566
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCG	5672	1.2243796613096458	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGC	5242	1.1315582130791895	No Hit
GAATCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTC	5087	1.0980993189496067	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2807	0.6059297794950945	No Hit
GCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCTGC	1589	0.3430076307864999	TruSeq Adapter, Index 13 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCT	1074	0.2318377567430465	No Hit
GAACTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTCT	1044	0.22536184175022395	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTC	818	0.17657661547096093	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGTAGCTATCGTA	729	0.1573647343255874	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTT	573	0.12368997636291028	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCG	570	0.12304238486362802	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCGGTAGCTATCGTATGC	524	0.11311264854130015	No Hit
GAATCTATCTCTTATACACATCTGACGCGGTAGCTATCGTATGCCGTCTTC	519	0.11203332937582973	No Hit
GCACAAGAGTGAGGAGAGCTAAAAGGACATGTAAGAAACCAATCAAGATCA	472	0.10188772922040776	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	2.1586383309408425E-4	0.0	0.0	0.07706338841458808	0.0
2	2.1586383309408425E-4	0.0	0.0	0.6624861037657446	0.0
3	2.1586383309408425E-4	0.0	0.0	0.8129431954323213	0.0
4	2.1586383309408425E-4	0.0	0.0	1.2312873039686565	0.0
5	2.1586383309408425E-4	0.0	0.0	2.8811345803067425	0.0
6	2.1586383309408425E-4	0.0	0.0	3.3394134979654835	0.0
7	2.1586383309408425E-4	0.0	0.0	3.863962612384108	0.0
8	2.1586383309408425E-4	0.0	0.0	4.586674725583102	0.0
9	2.1586383309408425E-4	0.0	0.0	4.783326677531813	0.0
10	4.317276661881685E-4	0.0	0.0	6.644504646469008	0.0
11	4.317276661881685E-4	0.0	0.0	7.25561515795836	0.0
12	4.317276661881685E-4	0.0	0.0	9.128449773882634	0.0
13	4.317276661881685E-4	0.0	0.0	9.442531651034528	0.0
14	4.317276661881685E-4	0.0	0.0	9.593204606534199	0.0
15	4.317276661881685E-4	0.0	0.0	9.943983335312085	0.0
16	4.317276661881685E-4	0.0	0.0	10.336639647710225	0.0
17	4.317276661881685E-4	0.0	0.0	10.735771875101186	0.0
18	4.317276661881685E-4	0.0	0.0	11.203117073749878	0.0
19	4.317276661881685E-4	0.0	0.0	11.545045385370909	0.0
20	4.317276661881685E-4	0.0	0.0	11.821998683230618	0.0
21	4.317276661881685E-4	0.0	0.0	12.160689037355237	0.0
22	4.317276661881685E-4	0.0	0.0	12.53564451543966	0.0
23	4.317276661881685E-4	0.0	0.0	12.891172248545617	0.0
24	4.317276661881685E-4	0.0	0.0	13.17935046572622	0.0
25	4.317276661881685E-4	0.0	0.0	13.41615309063043	0.0
26	4.317276661881685E-4	0.0	0.0	13.64129906854756	0.0
27	4.317276661881685E-4	0.0	0.0	13.916525455742518	0.0
28	4.317276661881685E-4	0.0	0.0	14.165848182966185	0.0
29	4.317276661881685E-4	0.0	0.0	14.41452331869057	0.0
30	6.475914992822528E-4	0.0	0.0	14.707234676366149	0.0
31	6.475914992822528E-4	0.0	0.0	14.999946034041727	0.0
32	6.475914992822528E-4	0.0	0.0	15.287044932056858	0.0
33	6.475914992822528E-4	0.0	0.0	15.551693991430206	0.0
34	6.475914992822528E-4	0.0	0.0	15.834907340449645	0.0
35	6.475914992822528E-4	0.0	0.0	16.13646911528208	0.0
36	6.475914992822528E-4	0.0	0.0	16.424647332462683	0.0
37	6.475914992822528E-4	0.0	0.0	16.72772015412678	0.0
38	6.475914992822528E-4	0.0	0.0	17.0370530269506	0.0
39	6.475914992822528E-4	0.0	0.0	17.344874852942763	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATCTCGA	20	7.02892E-4	45.000004	18
ACCGGGT	20	7.02892E-4	45.000004	4
ATCAGCT	20	7.02892E-4	45.000004	16
CGCGATC	20	7.02892E-4	45.000004	34
ATCCGCG	20	7.02892E-4	45.000004	43
CCGCGAT	20	7.02892E-4	45.000004	33
AATCGTC	20	7.02892E-4	45.000004	14
GTAAATC	20	7.02892E-4	45.000004	13
TGCAACG	20	7.02892E-4	45.000004	1
GGCCGAT	35	1.209828E-7	45.0	8
ACGGGTA	25	3.8871876E-5	45.0	5
AACGGCT	70	0.0	45.0	7
GACGGTC	25	3.8871876E-5	45.0	29
CCCGCGA	25	3.8871876E-5	45.0	32
CGTTTTT	1555	0.0	41.816723	1
ACGGCTG	630	0.0	41.785713	8
TACGGCT	555	0.0	41.756756	7
AACACGT	60	3.6379788E-12	41.250004	41
CGTCATA	55	6.002665E-11	40.909092	38
AAACGGC	90	0.0	40.0	6
>>END_MODULE